{"title":"Downregulation of a Phi class glutathione <i>S</i>-transferase gene in transgenic torenia yielded pale flower color.","authors":"Misako Akagi, Noriko Nakamura, Yoshikazu Tanaka","doi":"10.5511/plantbiotechnology.24.0409a","DOIUrl":null,"url":null,"abstract":"<p><p>The members of glutathione <i>S</i>-transferase (GST) belonging to the Phi class of the GST family are known to play a role in anthocyanin transport to the vacuole. We isolated a GST orthologue from the torenia petal cDNA library. Transgenic plants transcribing GST double stranded RNA were generated from a torenia cultivar having blue flowers. These plants exhibited a range of flower colors, from blue to almost white. Quantitative RT-PCR confirmed the downregulation of the GST transcript, accompanied by a decrease in anthocyanin levels in the petals of the transgenic plants, whereas flavone levels remained unchanged. These results suggest that GST is involved in anthocyanin transport in torenia petals, and that anthocyanins and flavones are likely transported to the vacuole through different mechanisms.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500598/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.5511/plantbiotechnology.24.0409a","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
The members of glutathione S-transferase (GST) belonging to the Phi class of the GST family are known to play a role in anthocyanin transport to the vacuole. We isolated a GST orthologue from the torenia petal cDNA library. Transgenic plants transcribing GST double stranded RNA were generated from a torenia cultivar having blue flowers. These plants exhibited a range of flower colors, from blue to almost white. Quantitative RT-PCR confirmed the downregulation of the GST transcript, accompanied by a decrease in anthocyanin levels in the petals of the transgenic plants, whereas flavone levels remained unchanged. These results suggest that GST is involved in anthocyanin transport in torenia petals, and that anthocyanins and flavones are likely transported to the vacuole through different mechanisms.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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