Physiology and Molecular Biology of Plants最新文献

{"title":"Integrating physiological and multi-omics methods to elucidate heat stress tolerance for sustainable rice production","authors":"Shilpy Singh, Afsana Praveen, Namrata Dudha, Pooja Bhadrecha","doi":"10.1007/s12298-024-01480-3","DOIUrl":"https://doi.org/10.1007/s12298-024-01480-3","url":null,"abstract":"<p>Heat stress presents unique challenges compared to other environmental stressors, as predicting crop responses and understanding the mechanisms for heat tolerance are complex tasks. The escalating impact of devastating climate changes heightens the frequency and intensity of heat stresses, posing a noteworthy threat to global agricultural productivity, especially in rice-dependent regions of the developing world. Humidity has been demonstrated to negatively affect rice yields worldwide. Plants have evolved intricate biochemical adaptations, involving intricate interactions among genes, proteins, and metabolites, to counter diverse external signals and ensure their survival. Modern-omics technologies, encompassing transcriptomics, metabolomics, and proteomics, have revolutionized our comprehension of the intricate biochemical and cellular shifts that occur in stressed agricultural plants. Integrating these multi-omics approaches offers a comprehensive view of cellular responses to heat stress and other challenges, surpassing the insights gained from multi-omics analyses. This integration becomes vital in developing heat-tolerant crop varieties, which is crucial in the face of increasingly unpredictable weather patterns. To expedite the development of heat-resistant rice varieties, aiming at sustainability in terms of food production and food security globally, this review consolidates the latest peer-reviewed research highlighting the application of multi-omics strategies.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141549404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved validation of protein interactions using bicistronic BiFC (Bi2FC)","authors":"Prakash Sivakumar, Vijayaraj Vaishnavi, Kothuri Gayatri, Gayathri R. Satheesh, Imran Siddiqi","doi":"10.1007/s12298-024-01477-y","DOIUrl":"https://doi.org/10.1007/s12298-024-01477-y","url":null,"abstract":"<p>Refolding based Bimolecular Fluorescence Complementation (BiFC) has emerged as an important in vivo technique to identify protein interactions. Significant improvements have been made to enhance the detection capacities of BiFC, however less attention has been paid to the detection of expression levels of proteins. Here we demonstrate development and validation of an improved method to identify protein interactions that incorporates an expression control based on bicistronic expression of the protein of interest and a fluorescent protein separated by a self-cleaving peptide. This method gives robust identification of positive interactions and more reliably identifies absence of interactions. We also show an earlier identified non-interacting pair in yeast two-hybrid (Y2H) to be interacting in vivo.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141504391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogen peroxide and salt stress in radish: effects on growth, physiology, and root quality","authors":"Juliane Maciel Henschel, Thiago Jardelino Dias, Vitória Stefany de Moura, Agnne Mayara de Oliveira Silva, Adriano Salviano Lopes, Daniel da Silva Gomes, Damiana Justino Araujo, João Batista Medeiros Silva, Oziel Nunes da Cruz, Diego Silva Batista","doi":"10.1007/s12298-024-01476-z","DOIUrl":"https://doi.org/10.1007/s12298-024-01476-z","url":null,"abstract":"<p>Hydrogen peroxide (H₂O₂) plays a central role in responses to salt stress, a major abiotic stress that impacts crop yield worldwide. Despite the evidence that H₂O₂ mitigates salt stress and improves post-harvest quality on several species, its effects on radish were not investigated so far. Thus, the objective of this study was to evaluate the exogenous application of H₂O₂ on salt stress mitigation of radish growth, physiology, and post-harvest quality. For this, radish plants were grown in pots for 30 days, being watered with non-saline (0.31 dS m⁻¹) or saline water (120 mM NaCl, 12.25 dS m⁻¹). Plants were leaf-sprayed weekly with water (control – 0 µM H₂O₂) or H₂O₂ (150 or 1500 µM) solutions. The experimental design was completely randomized in a 3 × 2 factorial scheme (H₂O₂ treatments × salt stress conditions). The growth, physiology (gas exchanges, photochemical efficiency, relative water content, electrolyte leakage, and the contents of chlorophylls and carotenoids), and post-harvest attributes of globular roots (color, anthocyanins, vitamin C, phenolic compounds, and soluble solids) were determined. Salt stress decreased gas exchanges and increased electrolyte leakage, which resulted in stunted radish growth, and increased the contents of antioxidants, such as anthocyanins, soluble solids, and vitamin C, improving globular root quality. Conversely, H₂O₂ did not mitigate salt stress effects on radish growth, photosynthetic capacity, and oxidative damages. Although H₂O₂ increased vitamin C under non-stressed condition, it was decreased under salt stress. Thus, we conclude that H₂O₂ did not mitigate salt stress on radish growth and quality.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141504389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphate solubilizing bacteria, Pseudomonas aeruginosa, improve the growth and yield of groundnut (Arachis hypogaea L.)","authors":"Monalisha Das Mohapatra, Ranjan Kumar Sahoo, Narendra Tuteja","doi":"10.1007/s12298-024-01478-x","DOIUrl":"https://doi.org/10.1007/s12298-024-01478-x","url":null,"abstract":"<p>For agricultural safety and sustainability, instead of synthetic fertilizers the eco-friendly and inexpensive biological applications include members of plant-growth-promoting rhizobacteria (PGPR) genera, <i>Pseudomonas</i> spp. will be an excellent alternative option to bioinoculants as they do not threaten the soil biota. The effect of phosphate solubilizing bacteria (PSB) <b><i>Pseudomonas aeruginosa</i></b><b> (MK 764942.1)</b> on groundnuts’ growth and yield parameters was studied under field conditions. The strain was combined with a single super phosphate and tested in different combinations for yield improvement. Integration of bacterial strain with P fertilizer gave significantly higher pod yield ranging from 7.36 to 13.18% compared to plots where sole inorganic fertilizers were applied. Similarly, the combined application of PSB and inorganic P fertilizer significantly influenced plant height and number of branches compared to sole. However, a higher influence of phosphorous application (both PSB and P fertilizer) observed both nodule dry weight and number of nodules. Combined with single super phosphate (100% P) topped in providing better yield attributing characters (pod yield, haulm yield, biomass yield, 1000 kernel weight, and shelling percentage) in groundnut. Higher oil content was also recorded with plants treated with <b><i>Pseudomonas aeruginosa</i></b> combined with single super phosphate (SSP) (100% P). Nutrients like nitrogen (N), phosphorous (P), and potassium (K) concentrations were positively influenced in shoot and kernel by combined application. In contrast, Ca, Mg, and S were found to be least influenced by variations of Phosphorous. Plants treated with <b><i>Pseudomonas aeruginosa</i></b> and lower doses of SSP (75% P) recorded higher shoot and kernel P. We found that co-inoculation with PSB and SSP could be an auspicious substitute for utilizing P fertilizer in enhancing yield and protecting nutrient concentrations in groundnut cultivation. Therefore, PSB can be a good substitute for bio-fertilizers to promote agricultural sustainability.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141504390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"De novo transcriptome profiling reveals the patterns of gene expression in plum fruits with bud mutations","authors":"Huiyan Liu, Haitian Fang, Guangdi Zhang, Jianshe Li, Xiangjun Zhang, Yu Li","doi":"10.1007/s12298-024-01472-3","DOIUrl":"https://doi.org/10.1007/s12298-024-01472-3","url":null,"abstract":"<p>Bud mutation is a common technique for plant breeding and can provide a large number of breeding materials. Through traditional breeding methods, we obtained a plum plant with bud mutations (named “By”) from an original plum variety (named “B”). The ripening period of “By” fruit was longer than that of “B” fruit, and its taste was better. In order to understand the characteristics of these plum varieties, we used transcriptome analysis and compared the gene expression patterns in fruits from the two cultivars. Subsequently, we identified the biological processes regulated by the differentially expressed genes (DEGs). Gene ontology (GO) analysis revealed that these DEGs were highly enriched for “single-organism cellular process” and “transferase activity”. KEGG analysis demonstrated that the main pathways affected by the bud mutations were plant hormone signal transduction, starch and sucrose metabolism. The <i>IAA</i>, <i>CKX</i>, <i>ARF</i>, and <i>SnRK2</i> genes were identified as the key regulators of plant hormone signal transduction. Meanwhile, <i>TPP</i>, the beta-glucosidase (EC3.2.1.21) gene, and <i>UGT72E</i> were identified as candidate DEGs affecting secondary metabolite synthesis. The transcriptome sequencing (RNA-seq) data were also validated using RT-qPCR experiments. The transcriptome analysis demonstrated that plant hormones play a significant role in extending the maturity period of plum fruit, with <i>IAA, CKX, ARF</i>, and <i>SnRK2</i> serving as the key regulators of this process. Further, <i>TPP,</i> beta-glucosidase (EC3.2.1.21), and <i>UGT72E</i> appeared to mediate the synthesis of various soluble secondary metabolites, contributing to the aroma of plum fruits. The expression of <i>BAG6</i> was upregulated in “B” as the fruit matured, but it was downregulated in “By”. This indicated that “B” may have stronger resistance, especially fungal resistance.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141504392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The auxin response factor (ARF) gene family in Cyclocarya paliurus: genome-wide identification and their expression profiling under heat and drought stresses","authors":"Ziyong Gao, Yazhu Wu, Muzi Li, Lan Ding, Junyi Li, Ying Liu, Yu Cao, Yangguang Hua, Qiaojun Jia, Dekai Wang","doi":"10.1007/s12298-024-01474-1","DOIUrl":"https://doi.org/10.1007/s12298-024-01474-1","url":null,"abstract":"<p>Auxin response factors (ARFs), as the main components of auxin signaling, play a crucial role in various processes of plant growth and development, as well as in stress response. So far, there have been no reports on the genome-wide identification of the ARF transcription factor family in <i>Cyclocarya paliurus</i>, a deciduous tree plant in the family Juglaceae. In this study, a total of 34 <i>CpARF</i> genes were identified based on whole genome sequence, and they were unevenly distributed on 16 chromosomes, with the highest distribution on chromosome 6. Domain analysis of CpARF proteins displayed that 31 out of 34 CpARF proteins contain a typical B3 domain (DBD domain), except CpARF12/ CpARF14/CpARF31, which all belong to Class VI. And 20 CpARFs (58.8%) contain an auxin_IAA binding domain, and are mainly distributed in classes I, and VI. Phylogenetic analysis showed that CpARF was divided into six classes (I–VI), each containing 4, 4, 1, 8, 4, and 13 members, respectively. Gene duplication analysis showed that there are 14 segmental duplications and zero tandem repeats were identified in the <i>CpARF</i> gene family of the <i>C. paliurus</i> genome. The Ka/Ks ratio of duplicate gene pairs indicates that <i>CpARF</i> genes are subjected to strong purification selection pressure. Synteny analysis showed that <i>C. paliurus</i> shared the highest homology in 74 <i>ARF</i> gene pairs with <i>Juglans regi</i>a, followed by 73, 51, 25, and 11 homologous gene pairs with <i>Populus trichocarpa</i>, <i>Juglans cathayensis</i>, <i>Arabidopsis</i>, and rice, respectively. Promoter analysis revealed that 34 <i>CpARF</i> genes had <i>cis-</i>elements related to hormones, stress, light, and growth and development except for <i>CpARF12</i>. The expression profile analysis showed that almost all <i>CpARF</i> genes were differentially expressed in at least one tissue, and several <i>CpARF</i> genes displayed tissue-specific expression. Furthermore, 24 out of the 34 <i>CpARF</i> genes have significantly response to drought stress (<i>P</i> &lt; 0.05), and most of them (16) being significantly down-regulated under moderate drought treatment. Meanwhile, the majority of <i>CpARF</i> genes (28) have significantly response to drought stress (<i>P</i> &lt; 0.05), and most of them (26) are significantly down-regulated under severe drought treatment. Furthermore, 32 out of the 34 <i>CpARF</i> genes have significantly response to high, middle, and low salt stress under salt treatment (<i>P</i> &lt; 0.05). Additionally, subcellular localization analysis confirmed that CpARF16 and CpARF32 were all localized to nucleus. Thus, our findings expand the understanding of the function of <i>CpARF</i> genes and provide a basis for further functional studies on <i>CpARF</i> genes in <i>C. paliurus</i>.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141504393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated analysis of miRNAs, transcriptome and phytohormones in the flowering time regulatory network of tea oil camellia","authors":"Haixiao Fan, Xiaoling Wang, Huiqi Zhong, Kehui Quan, Ruohan Yu, Shiying Ma, Siqiong Song, Mengfei Lin","doi":"10.1007/s12298-024-01473-2","DOIUrl":"https://doi.org/10.1007/s12298-024-01473-2","url":null,"abstract":"<p><i>Camellia oleifera</i> is a crucial cash crop in the southern region of China. Timely flowering is a crucial characteristic for maximizing crop productivity. Nevertheless, the cold temperature and wet weather throughout the fall and winter seasons in South China impact the timing of flowering and the yield produced by <i>C. oleifera</i>. This study examined the miRNAs, transcriptomes, and phytohormones that are part of the flowering time regulatory networks in distinct varieties of <i>C. oleifera</i> (Sep, Oct, and Nov). This study provides evidence that phytohormones significantly impact the timing of flowering in <i>C. oleifera</i> leaves. There is a positive correlation between the accumulation variations of zeatin (cZ), brassinolide (BL), salicylic acid (SA), 1-amino cyclopropane carboxylic acid (ACC), and jasmonic acid (JA) and flowering time. This means that blooming occurs earlier when the quantity of these substances in leaves increases. Abscisic acid (ABA), trans-zeatin-riboside (tZR), dihydrozeatin (dh-Z), and IP (N6-Isopentenyladenine) exhibit contrasting effects. Furthermore, both miR156 and miR172 play a crucial function in regulating flowering time in <i>C. oleifera</i> leaves by modulating the expression of <i>SOC1</i>, primarily through the miR156-SPL and miR172-AP2 pathways. These findings establish a strong basis for future research endeavors focused on examining the molecular network associated with the flowering period of <i>C. oleifera</i> and controlling flowering time management through external treatments.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141504394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification, and gene expression analysis of NBS-LRR domain containing R genes in Chenopodium quinoa for unveiling the dynamic contribution in plant immunity against Cercospora cf. chenopodii","authors":"Siddra Ijaz, Imran Ul Haq, Zakia Habib, Muti-Ullah, Irfan Afzal, Nasir Ahmad Khan, Abdullah","doi":"10.1007/s12298-024-01475-0","DOIUrl":"https://doi.org/10.1007/s12298-024-01475-0","url":null,"abstract":"<p>The plant R genes encode the NLR proteins comprising nucleotide-binding sites (NBS) and variable-length C-terminal leucine-rich repeat domains. The proteins act as intracellular immune receptors and recognize effector proteins of phytopathogens, which convene virulence. Among stresses, diseases contribute majorly to yield loss in crop plants, and R genes confer disease resistance against phytopathogens. We investigated the NLRome of <i>Chenopodium quinoa</i> for intraspecific diversity, characterization, and contribution to immune response regulation against phytopathogens. One eighty-three NBS proteins were identified and grouped into four distinct classes. Exon–intron organization displayed discrimination in gene structure patterns among NLR proteins. Thirty-eight NBS proteins revealed ontology with defense response, ADP binding, and inter alia cellular components. These proteins had shown functional homology with disease-resistance proteins involved in the plant-pathogen interaction pathway. Likewise, expression analysis demonstrated that NLRs encoding genes showed differential expression patterns. However, most genes displayed high expression levels in plant defense response with varying magnitude compared to ADP binding and cellular components. Twenty-four NBS genes were selected based on Heatmap analysis for quantitative polymerase chain reaction under Cercospora disease stress, and their progressive expression pattern provides insights into their functional role under stress conditions. The protein–protein interaction analysis revealed functional enrichment of NLR proteins in regulating hypersensitive, immune, and stress responses. This study, the first to identify and characterize NBS genes in <i>C. quinoa</i>, reveals their contribution to disease response and divulges their dynamic involvement in inducing plant immunity against phytopathogens.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141504395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon dioxide sensitization delays the postharvest ripening and fatty acids composition of Capsicum fruit by regulating ethylene biosynthesis, malic acid and reactive oxygen species metabolism","authors":"Arijit Ghosh, Mirza Hasanuzzaman, Masayuki Fujita, M. K. Adak","doi":"10.1007/s12298-024-01471-4","DOIUrl":"https://doi.org/10.1007/s12298-024-01471-4","url":null,"abstract":"<p>Present study would be significant in the sustenance of quality characters for postharvest storage of <i>Capsicum</i> fruit with CO₂-sensitization in biocompatible manner<b>.</b> The present experiment describes effects of CO₂ sensitization on delaying postharvest ripening through physiological attributes in <i>Capsicum</i> fruit<b>.</b> The experiment was conducted with acidified bicarbonate-derived CO₂ exposure for 2 h on <i>Capsicum</i> fruit, kept under white light at 25 °C through 7 days postharvest storage. Initially, fruits responded well to CO₂ as recorded sustenance of greenness and integrity of fruit coat resolved through scanning electron micrograph. Loss of water and accumulation of total soluble solids were marginally increased on CO₂-sensitized fruit as compared to non-sensitized (control) fruit. The ethylene metabolism biosynthetic genes like <i>CaACC synthase</i>, <i>CaACC oxidase</i> were downregulated on CO₂-sensitization. Accompanying ethylene metabolism cellular respiration was downregulated on CO₂ induction as compared to control through 7 days of storage. Fruit coat photosynthesis decarboxylating reaction by NADP malic enzyme was upregulated to maintain the reduced carbon accumulation as recorded on 7 days of storage under the same condition. CO₂-sensitization effectively reduced the lipid peroxides as oxidative stress products on ripening throughout the storage. Anti-oxidation reaction essentially downregulates the ROS-induced damages of biomolecules that otherwise are highly required for food preservation during postharvest storage. Thus, the major finding is that CO_2-sensitization maintains a higher ratio of unsaturated to saturated fatty acids in fruit coat during storage. Tissue-specific downregulation of ROS also maintained the nuclear stability under CO₂ exposure. These findings provide basic as well as applied insights for sustaining <i>Capsicum</i> fruit quality with CO₂ exposure under postharvest storage.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141504414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermotolerant plant growth-promoting bacteria enhance growth and nutrient uptake of lettuce under heat stress conditions by altering stomatal movement and chlorophyll fluorescence","authors":"Tsz Hei Chan, Hiran Anjana Ariyawansa, Hyungmin Rho","doi":"10.1007/s12298-024-01470-5","DOIUrl":"https://doi.org/10.1007/s12298-024-01470-5","url":null,"abstract":"<p>This study investigates the effects of selected PGPB on lettuce growth performance under heat-stress conditions. Bacterial plant growth-promoting potentials have been characterized and identified successfully in ongoing studies. Based on in vitro plant growth-promoting potential, the top five bacteria were ranked and identified as <i>Acinetobacter</i> sp. GRB12, <i>Bacillus</i> sp. GFB04, <i>Klebsiella</i> sp. LFB06, <i>Klebsiella</i> sp. GRB10, and <i>Klebsiella</i> sp. GRB04. They were mixed to inoculate on lettuce (<i>Lactuca sativa</i> L.) in temperature-controlled greenhouses. Another in-vivo chamber experiment was conducted by using <i>Bacillus</i> sp. GFB04 and <i>Klebsiella</i> sp. GFB10. Plant physiological traits (chlorophyll fluorescence and transpiration) and nutrient contents were measured at harvest, along with growth, development, and yield component analyses. Uninoculated plants under heat-stress condition showed poor growth performance. In contrast, plants with PGPB inoculation showed improved growth under heat-stress conditions, as the uptake of nutrients was facilitated by the symbionts. Inoculation also improved lettuce photosystem II efficiency and decreased total water use under heat stress. In conclusion, the current study suggests that PGPB inoculation successfully enhances lettuce heat-tolerance. PGPB application could potentially help improve sustainable production of lettuce with less fertilization under increasing temperatures.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141504415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}