Physiology and Molecular Biology of Plants最新文献

{"title":"Genome-wide characterization of <i>auxin response factor</i> (<i>ARF</i>) genes in bermudagrass and ectopically functional analysis of <i>CdARF6-B2</i> gene in <i>Arabidopsis</i>.","authors":"Zhuoting Chen, Bing Zhang","doi":"10.1007/s12298-024-01538-2","DOIUrl":"10.1007/s12298-024-01538-2","url":null,"abstract":"<p><p>Auxin response factors (ARFs) are important transcription factors that regulate the expression of auxin response genes, thus play crucial roles in plant growth and development. However, the functions of <i>ARF</i> genes in bermudagrass (<i>Cynodon dactylon</i> L.), a turfgrass species of great economic value, remain poorly understood. In this study, a total of 86 <i>CdARF</i> genes were identified from the <i>C. dactylon</i> genome and were categorized into five groups according to their phylogenetic relationships. The five groups of <i>CdARF</i> genes exhibited specific gene structure and protein domain characteristics, and showed distinct gene expression patterns in different organs, wild accessions and under different stress treatments. Among the 86 <i>CdARF</i> genes, the <i>CdARF6-B2</i> gene encoded an N-terminally truncated group V ARF protein with high sequence similarity to AtARF2 and OsARF24. The <i>CdARF6-B2</i> gene was highly expressed in the aboveground vegetative organs (leaf, shoot and stolon) and weakly expressed in the root. The CdARF6-B2 protein was localized in the nucleus but showed no transactivation activity, although its middle region had a strong transactivation activity. Ectopic expression of <i>CdARF6-B2</i> inhibited the vegetative growth of transgenic <i>Arabidopsis</i> plants possibly through down-regulating the expression of auxin transport-related <i>PIN3</i> gene and impeding the polar transport of auxin. These results not only established solid foundations to characterize the regulatory mechanism of auxin signaling in the growth and development of bermudagrass but also provided new insights into the function of <i>ARF</i> genes in plants.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-024-01538-2.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"30 12","pages":"1969-1981"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11685371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diploid inbred-based hybrids: fast-forward breeding approach in potatoes.","authors":"Salej Sood, Vikas Mangal, Ajay Kumar Thakur, Tanuja Buckseth, Babita Chaudhary, Vinod Kumar, Brajesh Singh","doi":"10.1007/s12298-024-01544-4","DOIUrl":"10.1007/s12298-024-01544-4","url":null,"abstract":"<p><p>Following the identification of the self-compatibility gene (<i>Sli</i>) in diploid potatoes two decades ago, the breeding of inbred based diploid hybrid potatoes made its way. Tetraploid potatoes have a long history of cultivation through domestication and selection. Tetrasomic inheritance, heterozygosity and clonal propagation complicate genetic studies, resulting in a low genetic gain in potato breeding. Diploid hybrid TPS potato breeding, similar to the developments in hybrid maize, was pursued as an alternative to the genetic improvement of potatoes. However, several challenges, like self-incompatibility and high inbreeding depression associated with diploid potatoes, must be overcome to develop inbred lines in potatoes. Moreover, the inbred lines must retain good fertility and vigour for hybrid breeding. Good progress has been made by creating di-haploids of popular varieties, mapping self-incompatibility inhibitor gene, understanding the genetic basis of inbreeding depression, and identifying genomic regions for deleterious alleles and fertility. Further, the genome sequencing of diploid inbred lines has revealed the genetics of key traits associated with potato breeding. This article discussed these insights and summarized the progress of diploid hybrid TPS potato breeding. Recent advances in genetic and genomic research and genome editing technology have shown promise for this technology's success and far-reaching implications.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"30 12","pages":"1955-1968"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11685360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142914815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA methylation in wheat: current understanding and future potential for enhancing biotic and abiotic stress tolerance.","authors":"Uzma Afreen, Kunal Mukhopadhyay, Manish Kumar","doi":"10.1007/s12298-024-01539-1","DOIUrl":"10.1007/s12298-024-01539-1","url":null,"abstract":"<p><p>DNA methylation is a paramount epigenetic mark that helps balance gene expression post-transcriptionally. Its effect on specific genes determines the plant's holistic development and acclimatization during adversities. <i>Triticum aestivum</i> L., an allohexaploid, is a dominant cereal crop with a large genome size. Changing environmental conditions exert a profound impact on its overall yield. Here, bibliometric science mapping was employed for a nuanced understanding of the prevailing research trends in the DNA methylation study of wheat. The detailed data obtained was used to delve deep into its fundamentals, patterns and mechanism of action, to accumulate evidence of the role of DNA methylation in the regulation of gene expressions across its entire genome. This review encapsulates the methylation/demethylation players in wheat during different stages of development. It also uncloaks the differential methylation dynamics while encountering biotic and abiotic constraints, focusing on the critical function it plays in fostering immunity. The study significantly contributes to broadening our knowledge of the regulatory mechanism and plasticity of DNA methylation in wheat. It also uncovers its potential role in improving breeding programs to produce more resilient wheat varieties, stimulating further research and development in the field.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-024-01539-1.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"30 12","pages":"1921-1933"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11685376/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142914821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous 24-Epibrassinolide alleviates salt stress in Okra <i>(Abelmoschus esculentus</i> L<i>.)</i> by increasing the expression of <i>SOS</i> pathway genes (<i>SOS1-3</i>) and <i>NHX1,4</i>.","authors":"Kazhal Yousefi, Rashid Jamei, Reza Darvishzadeh","doi":"10.1007/s12298-024-01515-9","DOIUrl":"10.1007/s12298-024-01515-9","url":null,"abstract":"<p><p>Given the rising population and food demand, it is imperative to devise solutions to enhance plant resilience against abiotic stresses. Salinity stress impacts plant growth but also hampers plant performance and productivity. Plant hormones have emerged as a viable remedy to mitigate the detrimental effects of salinity stress on plants. This study delved into the molecular investigation of the impact of 24-Epibrassinolide (EBL) on Okra plants (<i>Abelmoschus esculentus</i> L.) under two levels of salinity stress (75 and 150 mM), scrutinizing morphological, biochemical, and physiological parameters. Salinity stress led to a decline in growth, pigment and protein content, with EBL application ameliorating these indicators, albeit insignificantly impacting protein levels. Salinity triggered an upsurge in soluble sugars, proline, antioxidant enzymes (CAT, SOD, GP, and APX), and sodium levels, while reducing potassium and micronutrient concentrations (copper, iron, zinc). It downregulated the expression of <i>NHX1</i>, <i>NHX4, SOS1, SOS2,</i> and <i>SOS3</i> genes. EBL treatment bolstered potassium and micronutrient uptake, upregulated gene expression and enzymatic antioxidants, and elevated soluble sugar and proline levels. Analysis of the outcomes across these parameters suggests that EBL holds promise as an effective agent in mitigating salinity stress in Okra plants.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"30 12","pages":"2051-2063"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11685374/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification, evolution and expression analysis unveil the role of <i>Dendrocalamus farinosus NRT</i> genes in nitrogen utilization and nitrogen allocation.","authors":"Boya Wang, Siyuan Ren, Sen Chen, Suwei Hao, Gang Xu, Shanglian Hu, Ying Cao","doi":"10.1007/s12298-024-01541-7","DOIUrl":"10.1007/s12298-024-01541-7","url":null,"abstract":"<p><p>The rapid growth of Bamboo made the uptake and allocation of nitrogen much important. Nitrate is the main form that plant utilized nitrogen by nitrate transporters (NRTs) as well as ammonium salt. In this study, we identified 155 <i>DfNRT</i> genes which mapped to 32 chromosomes out of 35 chromosomes in <i>Dendrocalamus farinosus</i>. Collinearity analysis showed most <i>NRT</i> genes in <i>D. farinosus</i> paired with <i>NRT</i> genes in <i>D. farinosus</i> and <i>P. edulis</i>, which another two sequenced woody bamboo species, and the divergence was similar to the woody bamboo whole-genome duplication event. Through the ¹⁵N-nitrate trace analysis, we found that the nitrogen absorbed by roots in <i>D. farinosus</i> was preferentially distributed to above-ground parts, especially transported to leaves. <i>DfNPF2.13</i> and <i>DfNPF6.9</i> exhibited higher expression in leaf, and upregulated with extra N supply, suggesting they might be participating in N allocation between leaves in <i>D. farinosus</i>. This study provides a foundation for understanding the mechanism of nitrate transport and distribution in bamboo, and provide valuable information for improving bamboo nitrate absorption and promoting efficient nitrogen utilization.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-024-01541-7.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"30 12","pages":"1983-1999"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11685372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of alanine aminotransferase in crop resilience to climate change: a critical review.","authors":"Nisha Agrawal, Rati S Chunletia, Anand M Badigannavar, Suvendu Mondal","doi":"10.1007/s12298-024-01540-8","DOIUrl":"10.1007/s12298-024-01540-8","url":null,"abstract":"<p><p>Alanine aminotransferase (AlaAT) is a crucial enzyme present in various isoforms. It is playing vital role in both humans and plants. This concise review focuses on the role of AlaAT in plants, particularly on preharvest sprouting, hypoxia, nitrogen use efficiency, abiotic and biotic stress tolerance. The molecular genetics of AlaAT, including gene identification and the impact on plant yield and its physiology, is discussed. Notably, the major dormancy gene <i>Qsd1/SD1</i> governing AlaAT synthesis has been characterized and cloned in various crops. This review emphasizes the current understanding of AlaAT and its influence on plants, covering mechanisms regulating preharvest sprouting, hypoxia, drought tolerance, salt tolerance, biotic resistance and nitrogen use efficiency. Identifying a protein with multidimensional roles in crop plants is very important. Modern biotechnological approaches can alter such candidate gene/protein for superior varieties development. Overall, the review gives an understanding of the uncovered area of AlaAT and the challenge of climatic change triggers in plants. In the future, the potential of genome editing in AlaAT through enhancer insertion and rapid stabilization through speed breeding will impart resilience to crop plants against climate change.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"30 12","pages":"1935-1953"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11685375/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Survey of <i>Echinochloa</i> weed species in rice fields using a chloroplast DNA marker and spikelet characteristics identifies accessions with possible paternal inheritance and heteroplasmy.","authors":"Kausalya Sakthivel, Balasundari Dharbaranyam, Kalaimani Raju, Gayatri Venkataraman","doi":"10.1007/s12298-024-01525-7","DOIUrl":"10.1007/s12298-024-01525-7","url":null,"abstract":"<p><p>Hexaploid <i>Echinochloa. crus-galli</i> var. <i>crus-galli</i> and tetraploid <i>E. crus-galli</i> var. <i>oryzicola</i> are major weeds in rice fields. Supplementing molecular marker data with morphological and morphometric characterization is considered a reliable method for species identification. In the present study, <i>Echinochloa</i> weed accessions were collected from rice fields in Tamil Nadu, India [as plants (12) or seeds (10)]. Species level identification was carried out using the distinguishing chloroplastic DNA marker, <i>trn</i>T-L. Eight accessions were identified as <i>E. crus-galli</i> consistently across T₀ and T₁ generations and twelve others over a single generation (T₀ or T₁). Spikelet length is an important feature used to distinguish <i>E. crus-galli</i> and <i>E. oryzicola</i>. Accession P1, identified as <i>E. oryzicola,</i> using a chloroplast DNA marker (<i>trn</i>T-L insertion), has a spikelet length more consistent with <i>E. crus-galli</i> (≤ 4 mm) than <i>E. oryzicola</i>. Thus, 'inconsistent' accession P1 may have inherited DNA paternally from <i>E. oryzicola</i>, instead of the unknown maternal donor usually reported in literature for <i>E. crus-galli</i>. We also report, for the first time, the occurrence of heteroplasmic variation in <i>Echinochloa</i> (accession D4) over two successive generations (T₀ and T₁). We also suggest a caveat in the use of morphometric spikelet characters and chloroplastic DNA marker data alone to classify <i>Echinochloa</i> weed species conclusively. Occurrence of paternal plastid inheritance and heteroplasmy may have implications on weed fitness, including range expansion and selective advantage(s) in a rapidly changing environment (herbicide or stress tolerance).</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-024-01525-7.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"30 12","pages":"2017-2025"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11685369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of aluminium toxicity on GI tagged Kachai lemon seedlings.","authors":"Linthoingambi Ningombam, Budhindra Nath Hazarika, Siddhartha Singh, Lobsang Wangchu, Nangsol Dolma Bhutia, Punabati Heisnam, Shubranil Das, Tabalique Yumkhaibam, K H Anush Sheikh","doi":"10.1007/s12298-024-01536-4","DOIUrl":"10.1007/s12298-024-01536-4","url":null,"abstract":"<p><p>An experiment was performed to understand the effects of aluminium toxicity (AlCl₃·6H₂O) on Kachai lemon growth and development. The toxic effects of aluminium were assessed for 45 days in sand media. With untreated pots serving as the control, seedlings of 1 month old were exposed to three concentrations of AlCl₃·6H₂O: 300 μM, 600 μM and 900 μM. The nutrient Hoagland solution was also given to seedlings along with the Aluminium (Al) treatment. The outcome demonstrated that the chlorophyll content and carotenoids declined with the increase of the concentration levels of AlCl₃·6H₂O and interval of treatment. The contents of O₂ ^·- (Super oxide anion), H₂O₂ (Hydrogen peroxide) and OH (Hydroxyl radical) in seedlings increased with the higher concentration levels of aluminium and longer exposure to Al. Additionally, the activity of the enzymes catalase, superoxide dismutase, ascorbate peroxidase, peroxidase and glutathione reductase were increased in seedlings. Different non-enzymatic antioxidants' actions like tocopherol and Vitamin C played important defence mechanisms for the maintenance of tolerance in aluminium toxicity by increasing their content with an increase in the concentration of treatment levels in Kachai Lemon.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"30 12","pages":"2065-2075"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11685366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142914914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in soluble sugars and the expression of sugar transporter protein genes in strawberry crowns responding to <i>Colletotrichum fructicola</i> infection.","authors":"Si-Yu Chen, Xue Li, Ke Duan, Zi-Yi Li, Yun Bai, Xin-Yi Wang, Jing Yang, Xiao-Hua Zou, Mei-Ling Xu, Ying Wang, Qing-Hua Gao","doi":"10.1007/s12298-024-01523-9","DOIUrl":"10.1007/s12298-024-01523-9","url":null,"abstract":"<p><p>Strawberry (<i>Fragaria</i> × <i>ananassa</i>) production has been greatly hampered by anthracnose crown rot caused by <i>Colletotrichum fructicola</i>. Crown, the modified stem of strawberry, is a sink organ involved in sugar allocation. Some Sugar Transport Proteins (STPs) are involved in competition for sugars between pathogen and host. However, the chemical nature and involvement of strawberry <i>STP</i>s (<i>FaSTP</i>s) in crown rot development is largely elusive. To reveal how strawberry alters soluble sugars and upregulates <i>STP</i>s in responses to <i>C. fructicola</i>, high performance liquid chromatograph and <i>FaSTP</i> expression analysis were performed in the crowns of three strawberry varieties, following a genome-wide identification of <i>FaSTP</i>s. Both <i>C. fructicola</i> and mock treatment/control changed glucose, fructose and sucrose accumulation in strawberry crowns. With increasing infection duration, the hexose/sucrose ratio increased in all varieties; no such trend was clearly visible in mock-treated plants. A total of 56 <i>FaSTP</i> loci scattered across four subgenomes were identified in octoploid strawberry, and most of the protein products of these genes had a preferential location on plasma membrane. Putative fungal elicitor responsive cis-elements were identified in the promoters of more than half <i>FaSTP</i>s. At least eight members were upregulated in strawberry crowns during <i>C. fructicola</i> invasion. Of them, <i>FaSTP8</i> expression was markedly enhanced in three varieties at all time points except for 3 dpi in 'Jiuxiang'. RNAseq data retrieval further validated the expression responses of <i>FaSTP</i>s to <i>Colletotrichum</i> spp. In summary, this work identified several <i>FaSTP</i> candidate genes responsive to <i>Colletotrichum fructicola</i> invasion, demonstrated changes in soluble sugar levels in strawberry crowns as a result of infection, and laid the groundwork for future efforts to engineer strawberry resistance to <i>Colletotrichum</i> spp.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-024-01523-9.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"30 11","pages":"1777-1793"},"PeriodicalIF":3.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11646252/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142838759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arabidopsis ubiquitin ligase PUB41 positively regulates ABA-mediated seed dormancy and drought response.","authors":"Avinash Sharma, Shalev Goldfarb, Dina Raveh, Dudy Bar-Zvi","doi":"10.1007/s12298-024-01526-6","DOIUrl":"10.1007/s12298-024-01526-6","url":null,"abstract":"<p><p>Seed germination is a tightly regulated, non-reversible developmental process, and it is crucial to prevent premature germination under conditions that may not allow the plant's life cycle to be completed. The plant hormone ABA is the key regulator of seed dormancy and inhibition of germination. ABA is also involved in the plant response to drought. Here we report on the involvement of <i>Arabidopsis thaliana PUB41</i>, encoding a U-BOX E3 ubiquitin ligase, in regulating ABA signaling, seed dormancy, germination, and drought resilience. <i>AtPUB41</i> is expressed in most vegetative and reproductive tissues. AtPUB41 protein is localized in the cytosol and nucleus. <i>pub41</i> T-DNA insertion mutants display reduced seed dormancy, and their germination is less inhibited by exogenous ABA than seeds of wild-type plants. <i>pub41</i> mutant plants are also hypersensitive to drought. ABA induces <i>AtPUB41</i> promoter activity and steady-state mRNA levels in the roots. Our data suggest that <i>AtPUB41</i> is a positive regulator of ABA signaling.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-024-01526-6.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"30 11","pages":"1819-1827"},"PeriodicalIF":3.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11646239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142838732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}