Physiology and Molecular Biology of Plants最新文献

{"title":"Breeding rice for yield improvement through CRISPR/Cas9 genome editing method: current technologies and examples","authors":"Balakrishnan Rengasamy, Mrinalini Manna, Nargis Begum Thajuddin, Muthukrishnan Sathiyabama, Alok Krishna Sinha","doi":"10.1007/s12298-024-01423-y","DOIUrl":"https://doi.org/10.1007/s12298-024-01423-y","url":null,"abstract":"<p>The impending climate change is threatening the rice productivity of the Asian subcontinent as instances of crop failures due to adverse abiotic and biotic stress factors are becoming common occurrences. CRISPR-Cas9 mediated genome editing offers a potential solution for improving rice yield as well as its stress adaptation. This technology allows modification of plant’s genetic elements and is not dependent on foreign DNA/gene insertion for incorporating a particular trait. In this review, we have discussed various CRISPR-Cas9 mediated genome editing tools for gene knockout, gene knock-in, simultaneously disrupting multiple genes by multiplexing, base editing and prime editing the genes. The review here also presents how these genome editing technologies have been employed to improve rice productivity by directly targeting the yield related genes or by indirectly manipulating various abiotic and biotic stress responsive genes. Lately, many countries treat genome-edited crops as non-GMOs because of the absence of foreign DNA in the final product. Thus, genome edited rice plants with improved yield attributes and stress resilience are expected to be accepted by the public and solve food crisis of a major portion of the globe.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"5 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140075436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the potential of Sargassum tenerrimum extract: metabolic profiling and pathway analysis of groundnut (Arachis hypogaea) in response to Sargassum extract and Sclerotium rolfsii","authors":"","doi":"10.1007/s12298-024-01418-9","DOIUrl":"https://doi.org/10.1007/s12298-024-01418-9","url":null,"abstract":"<h3>Key message</h3> <p>The differential metabolite profiling and pathway analysis of groundnut in response to <em>Sargassum</em> extract and <em>S. rolfsii</em> help in understanding the groundnut- <em>S. rolfsii</em> interactions and the potential role of the <em>Sargassum</em> extract towards these interactions.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"88 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139987908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drought stress in Lens culinaris: effects, tolerance mechanism, and its smart reprogramming by using modern biotechnological approaches","authors":"Sakshi Saini, Priyanka Sharma, Jyoti Sharma, Pooja Pooja, Asha Sharma","doi":"10.1007/s12298-024-01417-w","DOIUrl":"https://doi.org/10.1007/s12298-024-01417-w","url":null,"abstract":"<p>Among legumes, lentil serves as an imperative source of dietary proteins and are considered an important pillar of global food and nutritional security. The crop is majorly cultivated in arid and semi-arid regions and exposed to different abiotic stresses. Drought stress is a polygenic stress that poses a major threat to the crop productivity of lentils. It negatively influenced the seed emergence, water relations traits, photosynthetic machinery, metabolites, seed development, quality, and yield in lentil. Plants develop several complex physiological and molecular protective mechanisms for tolerance against drought stress. These complicated networks are enabled to enhance the cellular potential to survive under extreme water-scarce conditions. As a result, proper drought stress-mitigating novel and modern approaches are required to improve lentil productivity. The currently existing biotechnological techniques such as transcriptomics, genomics, proteomics, metabolomics, CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/cas9), and detection of QTLs (quantitative trait loci), proteins, and genes responsible for drought tolerance have gained appreciation among plant breeders for developing climate-resilient lentil varieties. In this review, we critically elaborate the impact of drought on lentil, mechanisms employed by plants to tolerate drought, and the contribution of omics approaches in lentils for dealing with drought, providing deep insights to enhance lentil productivity and improve resistance against abiotic stresses. We hope this updated review will directly help the lentil breeders to develop resistance against drought stress.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\u0000","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"40 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139987730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of two Erwinia sp. on the response of diverse Pisum sativum genotypes under salt stress","authors":"Houda Ilahi, Elisa Zampieri, Cristiana Sbrana, Francesca Brescia, Luca Giovannini, Roghayyeh Mahmoudi, Gholamreza Gohari, Mustapha Missbah El Idrissi, Mohamed Najib Alfeddy, Martino Schillaci, Lahcen Ouahmane, Alice Calvo, Fabiano Sillo, Vasileios Fotopoulos, Raffaella Balestrini, Bacem Mnasri","doi":"10.1007/s12298-024-01419-8","DOIUrl":"https://doi.org/10.1007/s12298-024-01419-8","url":null,"abstract":"<p>Currently, salinization is impacting more than 50% of arable land, posing a significant challenge to agriculture globally. Salt causes osmotic and ionic stress, determining cell dehydration, ion homeostasis, and metabolic process alteration, thus negatively influencing plant development. A promising sustainable approach to improve plant tolerance to salinity is the use of plant growth-promoting bacteria (PGPB). This work aimed to characterize two bacterial strains, that have been isolated from pea root nodules, initially called PG1 and PG2, and assess their impact on growth, physiological, biochemical, and molecular parameters in three pea genotypes (Merveille de Kelvedon, Lincoln, Meraviglia d’Italia) under salinity. Bacterial strains were molecularly identified, and characterized by in vitro assays to evaluate the plant growth promoting abilities. Both strains were identified as <i>Erwinia</i> sp., demonstrating in vitro biosynthesis of IAA, ACC deaminase activity, as well as the capacity to grow in presence of NaCl and PEG. Considering the inoculation of plants, pea biometric parameters were unaffected by the presence of the bacteria, independently by the considered genotype. Conversely, the three pea genotypes differed in the regulation of antioxidant genes coding for catalase (<i>PsCAT</i>) and superoxide dismutase (<i>PsSOD</i>). The highest proline levels (212.88 μmol g⁻¹) were detected in salt-stressed Lincoln plants inoculated with PG1, along with the up-regulation of <i>PsSOD</i> and <i>PsCAT</i>. Conversely, PG2 inoculation resulted in the lowest proline levels that were observed in Lincoln and Meraviglia d’Italia (35.39 and 23.67 μmol g⁻¹, respectively). Overall, this study highlights the potential of these two strains as beneficial plant growth-promoting bacteria in saline environments, showing that their inoculation modulates responses in pea plants, affecting antioxidant gene expression and proline accumulation.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"30 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139969120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro micropropagation and conservation of endangered medicinal plant Nepeta asterotricha Rech.f. (Lamiaceae): genetic fidelity, phytochemical and biological assessment","authors":"Mahdieh Zamani, Ali Sonboli, Mostafa Goldansaz, Mohammad Hossein Mirjalili","doi":"10.1007/s12298-024-01416-x","DOIUrl":"https://doi.org/10.1007/s12298-024-01416-x","url":null,"abstract":"<p>An efficient in vitro protocol was introduced for the conservation of <i>Nepeta asterotricha</i>, a vulnerable and endangered medicinal species found in the central of Iran for the first time. Growth, phytochemical, and biological traits of in vitro regenerated plant (RP) and acclimated plant (AP) were compared to the mother plant (MP). In addition, the genetic stability of AP was assessed by using inter-simple sequence repeats (ISSR) markers. The highest number of lateral branches (4.25) was obtained from the medium with 3 mg/mL kinetin (KIN), while the highest length of lateral branches (13.25 cm) was achieved on the medium culture fortified with 3 mg/mL thidiazuron (TDZ) and 6-benzylaminopurine (BAP). The highest number of leaves (20.25) and main branch length (12.25 cm) were obtained from the medium containing 3 mg/mL TDZ. The highest number of roots (46.25) and root length (2.25 cm) was measured from the medium fortified with 1 mg/mL indole-3-butyric acid (IBA) and 0.6 mg/mL indole-3-acetic acid (IAA), respectively. RP was successfully acclimated (85%) in vivo. Molecular analysis showed that the AP was true to the type of the MP. <i>cis</i>-Sabinene hydrate (26.8–57.7), 1,8-cineole (6.2–24.1), 4aα,7β,7aα-nepetalactone (4.1–12.3), and terpinene-4-ol (3.2–15.0) were the major essential oils compounds. The studied samples contained rosmarinic acid (2.55–5.97 mg/g DW), cichoric acid (1.68–12.7 mg/g DW), chlorogenic acid (1.91–64.21 mg/g DW), rutin (0.59–1.09 mg/g DW), apigenin (0.52–0.72 mg/g DW), betulinic acid (0.17–2.20 mg g DW), oleanolic acid (0.84–5.37 mg/g DW) and ursolic acid (3.46–15.70 mg/g DW). Acclimated plant exhibited the highest antioxidant activity (IC₅₀ = 196.4 μg/mL), while the methanolic extract of MP displayed the highest antibacterial activity (MIC = 8 mg/mL) against <i>Staphylococcus aureus</i>.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"135 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139952968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"VvATG6 contributes to copper stress tolerance by enhancing the antioxidant ability in transgenic grape calli","authors":"Jiaxin Xia, Zicheng Wang, Siyu Liu, Xiang Fang, Abdul Hakeem, Jinggui Fang, Lingfei Shangguan","doi":"10.1007/s12298-024-01415-y","DOIUrl":"https://doi.org/10.1007/s12298-024-01415-y","url":null,"abstract":"<p>Autophagy, a conserved degradation and reuse process, plays a crucial role in plant cellular homeostasis during abiotic stress. Although numerous autophagy–related genes (<i>ATGs</i>) that regulate abiotic stress have been identified, few functional studies have shown how they confer tolerance to copper (Cu) stress. Here, we cloned a novel <i>Vitis vinifera</i> <i>ATG6</i> gene (<i>VvATG6</i>) which was induced by 0.5 and 10 mM Cu stress based on transcriptomic data, and transgenic <i>Arabidopsis thaliana</i>, tobacco (<i>Nicotiana tabacum</i>), and grape calli were successfully obtained through <i>Agrobacterium</i>-mediated genetic transformation. The overexpression of <i>VvATG6</i> enhanced the tolerance of transgenic lines to Cu. After Cu treatment, the lines that overexpressed <i>VvATG6</i> grew better and increased their production of biomass compared with the wild-type. These changes were accompanied by higher activities of antioxidant enzymes and a lower accumulation of deleterious malondialdehyde and hydrogen peroxide in the transgenic plants. The activities of superoxide dismutase, peroxidase, and catalase were enhanced owing to the elevation of corresponding antioxidant gene expression in the <i>VvATG6</i> overexpression plants under Cu stress, thereby promoting the clearance of reactive oxygen species (ROS). Simultaneously, there was a decrease in the levels of expression of <i>RbohB</i> and <i>RbohC</i> that are involved in ROS synthesis in transgenic plants under Cu stress. Thus, the accelerated removal of ROS and the inhibition of its synthesis led to a balanced ROS homeostasis environment, which alleviated the damage from Cu. This could benefit from the upregulation of other <i>ATGs</i> that are necessary for the production of autophagosomes under Cu stress. To our knowledge, this study is the first to demonstrate the protective role of <i>VvATG6</i> in the Cu tolerance of plants.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"149 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139903453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of nitric oxide mediated defense signaling and its microRNA mediated regulation during Phytophthora capsici infection in black pepper","authors":"Srinivasan Asha, Divya Kattupalli, Mallika Vijayanathan, E. V. Soniya","doi":"10.1007/s12298-024-01414-z","DOIUrl":"https://doi.org/10.1007/s12298-024-01414-z","url":null,"abstract":"<p>Nitric oxide plays a significant role in the defense signaling during pathogen interaction in plants. Quick wilt disease is a devastating disease of black pepper, and leads to sudden mortality of pepper vines in plantations. In this study, the role of nitric oxide was studied during <i>Phytophthora capsici</i> infection in black pepper variety Panniyur-1. Nitric oxide was detected from the different histological sections of <i>P. capsici</i> infected leaves. Furthermore, the genome-wide transcriptome analysis characterized typical domain architect and structural features of nitrate reductase (<i>NR</i>) and nitric oxide associated 1 (<i>NOA1</i>) gene that are involved in nitric oxide biosynthesis in black pepper. Despite the upregulation of nitrate reductase (<i>Pn1_NR</i>), a reduced expression of <i>Pn1_NOA1</i> was detected in the <i>P. capsici</i> infected black pepper leaf. Subsequent sRNAome-assisted in silico analysis revealed possible microRNA mediated regulation of <i>Pn1_NOA</i> mRNAs. Furthermore, sRNA/miRNA mediated cleavage on <i>Pn1_NOA1</i> mRNA was validated through modified 5' RLM RACE experiments. Several hormone-responsive cis-regulatory elements involved in stress response was detected from the promoter regions of <i>Pn_NOA1, Pn_NR1</i> and <i>Pn_NR2</i> genes. Our results revealed the role of nitric oxide during stress response of <i>P. capsici</i> infection in black pepper, and key genes involved in nitric oxide biosynthesis and their post-transcriptional regulatory mechanisms.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"62 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139756753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional characterization of GhNAC2 promoter conferring hormone- and stress-induced expression: a potential tool to improve growth and stress tolerance in cotton","authors":"Ram Naresh, Richa Srivastava, Samatha Gunapati, Aniruddha P. Sane, Vidhu A. Sane","doi":"10.1007/s12298-024-01411-2","DOIUrl":"https://doi.org/10.1007/s12298-024-01411-2","url":null,"abstract":"<p>The GhNAC2 transcription factor identified from <i>G. herbaceum</i> improves root growth and drought tolerance through transcriptional reprogramming of phytohormone signaling. The promoter of such a versatile gene could serve as an important genetic engineering tool for biotechnological application. In this study, we identified and characterized the promoter of <i>GhNAC2</i> to understand its regulatory mechanism. <i>GhNAC2</i> transcription factor increased in root tissues in response to GA, ethylene, auxin, ABA, mannitol, and NaCl. In silico analysis revealed an overrepresentation of <i>cis</i>-regulatory elements associated with hormone signaling, stress responses and root-, pollen-, and seed-specific promoter activity. To validate their role in <i>GhNAC2</i> function/regulation, an 870-bp upstream regulatory sequence was fused with the GUS reporter gene (<i>uidA</i>) and expressed in <i>Arabidopsis</i> and cotton hairy roots for <i>in planta</i> characterization. Histochemical GUS staining indicated localized expression in root tips, root elongation zone, root primordia, and reproductive tissues under optimal growth conditions. Mannitol, NaCl, auxin, GA, and ABA, induced the promoter-driven GUS expression in all tissues while ethylene suppressed the promoter activity. The results show that the 870 nt fragment of the <i>GhNAC2</i> promoter drives root-preferential expression and responds to phytohormonal and stress signals. In corroboration with promoter regulation, GA and ethylene pathways differentially regulated root growth in <i>GhNAC2</i>-expressing <i>Arabidopsis</i>. The findings suggest that differential promoter activity governs the expression of <i>GhNAC2</i> in root growth and stress-related functions independently through specific promoter elements. This multifarious promoter can be utilized to develop yield and climate resilience in cotton by expanding the options to control gene regulation.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"62 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139756548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-QTL and ortho analysis unravels the genetic architecture and key candidate genes for cold tolerance at seedling stage in rice","authors":"Anita Kumari, Priya Sharma, Mamta Rani, Vijay Laxmi, Sahil, Chandan Sahi, Vanisree Satturu, Surekha Katiyar-Agarwal, Manu Agarwal","doi":"10.1007/s12298-024-01412-1","DOIUrl":"https://doi.org/10.1007/s12298-024-01412-1","url":null,"abstract":"<p>Rice, a critical cereal crop, grapples with productivity challenges due to its inherent sensitivity to low temperatures, primarily during the seedling and booting stages. Recognizing the polygenic complexity of cold stress signaling in rice, a meta-analysis was undertaken, focusing on 20 physiological traits integral to cold tolerance. This initiative allowed the consolidation of genetic data from 242 QTLs into 58 meta-QTLs, thereby significantly constricting the genetic and physical intervals, with 84% of meta-QTLs (MQTLs) being reduced to less than 2 Mb. The list of 10,505 genes within these MQTLs, was further refined utilizing expression datasets to pinpoint 46 pivotal genes exhibiting noteworthy differential regulation during cold stress. The study underscored the presence of several TFs such as WRKY, NAC, CBF/DREB, MYB, and bHLH, known for their roles in cold stress response. Further, ortho-analysis involving maize, barley, and <i>Arabidopsis</i> identified OsWRKY71, among others, as a prospective candidate for enhancing cold tolerance in diverse crop plants. In conclusion, our study delineates the intricate genetic architecture underpinning cold tolerance in rice and propounds significant candidate genes, offering crucial insights for further research and breeding strategies focused on fortifying crops against cold stress, thereby bolstering global food resilience.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"36 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139677395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guest Editorial: Special Issue: Cellular signals and molecular physiology of plant responses.","authors":"Neeti Sanan-Mishra, Renu Deswal","doi":"10.1007/s12298-024-01437-6","DOIUrl":"https://doi.org/10.1007/s12298-024-01437-6","url":null,"abstract":"","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"30 2","pages":"287-288"},"PeriodicalIF":3.5,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11016015/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140849837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}