Physiology and Molecular Biology of Plants最新文献

{"title":"Temperature stress and its effects on phytochemical dynamics and antioxidant activity in <i>Withania somnifera</i> (L). dunal.","authors":"Ankita Singh, Usha Mina","doi":"10.1007/s12298-025-01594-2","DOIUrl":"10.1007/s12298-025-01594-2","url":null,"abstract":"<p><p>Elevated temperature stress limits the growth, metabolism and productivity of medicinal plants. However, the response of <i>Withania somnifera</i> (L.) Dunal (Ashwagandha) which has diverse therapeutic properties, to elevated temperature stress remains unexplored. This study investigated the effects of elevated temperature stress exposure on the phytochemical content and antioxidant activity of Ashwagandha four varieties namely: Vallabh 01, Vallabh 02, Pratap, and Chetak, across leaf, stem, root, and fruit parts. The selected varieties were exposed to ambient temperature (10.4 ± 2.6 to 31.6 ± 5.9 ℃) and elevated temperature (ET) levels (13.5 ± 3.5 to 34.3 ± 5.6 ℃) in net house and control environment facility chamber, respectively from November 2021 to April 2022. Fruiting stage samples of different parts of all varieties from both treatments were analysed for total phytochemical content, total flavonoid content (TFC) and antioxidant activity. The results revealed the variable response of varieties and their parts to ET stress. ET stress enhanced the cumulative (root + stem + leaf + fruit) phytochemical content of Vallabh 02, Pratap and Chetak by 112.9%, 15.2%, and 84.9%, respectively, and suppressed in the Vallabh 01 (3.6%). TFC and antioxidant activity were significantly (p < 0.05) higher under ET stress in all the varieties in the following order Pratap > Chetak > Vallabh 01 > Vallabh 02. The findings indicate that ET stress exposure significantly alters as well as enhances Ashwagandha varieties' total phytochemical content and antioxidant activity. Among the four varieties, Pratap is the most tolerant to ET stress. This study indicates that climate change associated ET levels up to 2 ℃ may enhance therapeutic potential of Ashwagandha varieties through synthesis of novel and unique phytochemicals. Novel knowledge of ET levels exposure to favour the production of specific phytochemicals can aid in optimizing the cultivation of medicinal plants for higher yields of desired medicinal compounds.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01594-2.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 4","pages":"675-692"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pigments to precision: RUBY aiding genetic transformation and genome editing in wheat and barley.","authors":"Manas Ranjan Prusty, Arava Shatil-Cohen, Rakesh Kumar, Davinder Sharma, Anna Minz-Dub, Smadar Ezrati, Avigail Hihinashvili, Amir Sharon","doi":"10.1007/s12298-025-01591-5","DOIUrl":"10.1007/s12298-025-01591-5","url":null,"abstract":"<p><p>Genetic engineering of wheat is complex due to its large genome size, the presence of numerous genes with high sequence similarities, and a multitude of repetitive elements. In addition, genetic transformation of wheat has been difficult, mainly due to poor regeneration in tissue cultures. Recent advances in plant biotechnology, particularly the use of the regenerative genes GROWTH-REGULATING FACTOR (<i>GRF</i>) and GRF-INTERACTING FACTOR (<i>GIF</i>), have provided new tools for wheat transformation and regeneration. Another transformative tool is the RUBY system that involves genetic engineering of three betalain biosynthesis genes, providing a noninvasive, visually detectable red pigment. In this study, we used the <i>GRF4-GIF1</i> chimera along with the RUBY system to advance transformation and gene editing in wheat and barley. The <i>GRF4-GIF1</i> chimera significantly aided wheat regeneration; however, it had an opposite effect in barley, where it inhibited the regeneration process. Therefore, we generated RUBY transgenic barley lines using constructs that did not include the <i>GRF4-GIF1</i> chimera. Additionally, we used the RUBY cassette for fast assessment of gene editing by knockingout the first betalain biosynthetic gene in RUBY- positive transgenic wheat plants, resulting in a change of leaf color from red to green. The edited RUBY wheat lines lost more than just the red color. They also lost betalain-related traits, such as being less likely to get leaf rust (<i>Puccinia triticina</i>) and salt stress. Importantly, the loss of RUBY did not affect plant viability, making it a useful tool for genome editing and a viable alternative to destructive methods.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01591-5.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 4","pages":"545-554"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116407/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cd²⁺ and Zn²⁺ regulating uptake and accumulation of TDCPP and TMPP in rice (<i>Oryza sativa</i> L.) in transcript and protein level.","authors":"Mengyao Wu, Haiou Wang, Wenxuan Wang, Xiaoyu Ren, Juming Zhang","doi":"10.1007/s12298-025-01589-z","DOIUrl":"10.1007/s12298-025-01589-z","url":null,"abstract":"<p><p>Hydroponic experiments and computational simulations were conducted to investigate the changes in the gene expression, structure, and binding mode of the rice transporter protein OsTIL to OPEs by the presence of Cd²⁺ and Zn²⁺. OPEs and Zn²⁺ were observed to promote seedling growth and OPEs alleviated the suppressive effect of Cd²⁺ on seedlings. Usually, Cd²⁺ and Zn²⁺ inhibited the accumulations of OPEs in plants accompanied by the decrease of RCF (root concentration factor) and TF (transport coefficient). In particular, Zn²⁺ promoted TDCPP accumulation only in roots accompanied by the increase of RCF and the decrease of TF. Furthermore, Cd²⁺ and Zn²⁺ affected the gene expressions of OPEs transporter-OsTIL response in the accumulation of OPEs in both single and compound pollution. After molecular dynamics simulation analysis, RMSD of the protein backbone, binding pocket, and ligand only in TDCPP-OsTIL complex also were significantly affected by two metal ions. Furthermore, two metal ions can press the interaction of OPEs and OsTIL by reducing the stability of protein structure and the numbers of HB (hydrogen bonds) and enlarging the pocket. However, Zn²⁺ extra supports an enlarged entrance for TDCPP, which could facilitate the entry of the coordination complex of Zn²⁺ with TDCPP into the pocket and improve TDCPP capacity of OsTIL.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01589-z.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 4","pages":"555-570"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring pharmacological potential of <i>Aegle marmelos</i>: integrating traditional knowledge with modern physiology and molecular biology.","authors":"Manish Thakur, Tejinder Kaur, Ranbir Chander Sobti","doi":"10.1007/s12298-025-01586-2","DOIUrl":"https://doi.org/10.1007/s12298-025-01586-2","url":null,"abstract":"<p><p><i>Aegle marmelos</i> is a plant that holds considerable medicinal value. It is indigenous to India, Iran, Myanmar, Pakistan, Bangladesh, and various Southeast Asian countries. The sacred tree, which has its historical roots in the Vedic era dating back to 2000 B.C., holds a significant place in various traditional systems of medicine due to its extensive therapeutic properties that have been recognized over time. The plant exhibits a diverse array of bioactive compounds, namely flavonoids, alkaloids, polyphenols, terpenoids, carotenoids, and coumarins, which are present in various parts of the plant, including leaves, flowers, bark, and fruit. These compounds are responsible for the plant's extensive range of medicinal properties. It has been observed that <i>A. marmelos</i> demonstrates various biological activities including anti-proliferative, anti-pyretic, anti-inflammatory, anti-fungal, anti-diarrhoeal, and antimicrobial properties. Recent scientific investigations have provided confirmation regarding the effectiveness of the substance under investigation against a wide range of disease-causing microorganisms. These microorganisms include bacteria such as <i>Micrococcus luteus</i> and <i>Streptococcus faecalis</i>, as well as fungi like <i>Aspergillus fumigatus</i> and <i>Candida albicans</i>. The phytochemical profile of <i>A. marmelos</i> includes the presence of marmenol, marmin, marmelosin, and several other compounds that are known to contribute to its medicinal properties. The present review aims to comprehensively synthesize the existing body of research on <i>Aegle marmelos,</i> focusing on its botanical characteristics, phytochemical composition, and wide-ranging medicinal applications.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01586-2.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 4","pages":"521-543"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116412/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated transcriptome and metabolome analyses reveal regulation mechanism of biomass and non‑structural carbohydrate allocation in <i>Emmenopterys henryi</i> Oliv. under shade.","authors":"Wenna Liu, Ruili Luo, Hongwei Wang, Yu Jing, Huaqiang Zhao, Weina Zou, Meifang Hou, Lili Song","doi":"10.1007/s12298-025-01588-0","DOIUrl":"10.1007/s12298-025-01588-0","url":null,"abstract":"<p><p>The adaptability of <i>Emmenopterys henryi</i> Oliv. to shade in the forest is a crucial intrinsic driving force for its natural renewal. Elucidating the influence of shade on biomass and non-structural carbohydrate (NSC) accumulation and allocation in leaf, stem and root will help to understand the endangerment mechanism of <i>E. henryi.</i> Results showed that <i>E. henryi</i> invested more biomass in leaf than in stem and root under shade. The biomass was positively correlated with the NSC pool in leaf, stem and root, respectively. The biomass fraction of leaf, stem or root was positively correlated with NSC fraction in leaf, stem or root of <i>E. henryi</i>, respectively. Starch and sucrose metabolism was proved to be the commonly enriched pathway in leaf, stem and root of <i>E. henryi</i> under shade, the key genes that were regulated differentially by shade. The hub genes regulating accumulation and distribution of biomass and NSC in leaf, stem and root of <i>E. henryi</i> under shade mainly correlated with photosynthesis, respiration, monosaccharides transportation, and cell expansion. Further research into these hub genes will be helpful for illumination of the adaptation mechanism of <i>E. henryi</i> to shade.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01588-0.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 4","pages":"571-590"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116963/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144183696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological, biochemical, and biophysical changes in chia seeds during accelerated aging: implications for lipid composition and seed quality.","authors":"María Emilia Rodríguez, Ethel Pérez, Martín Moisés Acreche, Aline Schneider-Teixeira, Lorena Deladino, Vanesa Ixtaina","doi":"10.1007/s12298-025-01595-1","DOIUrl":"10.1007/s12298-025-01595-1","url":null,"abstract":"<p><p>Chia, an oilseed native to Mexico and Guatemala, is prized for its nutrition and versatile uses in food and industry. Ex situ conservation of chia seeds is vital, yet their high lipid content complicates long-term storage. This study investigates artificial aging's impact on chia seed quality, emphasizing oxidative stress effects on lipid composition, antioxidants, and physiological properties. Two chia genotypes -one with mixed seed colors (MN) and another exclusively white (WN)- were subjected to accelerated aging to analyze germination, growth, electrical conductivity, and biochemical and biophysical changes over time. Accelerated aging revealed stress tolerance in chia seeds but significantly impacted germination and biochemical composition. Germination decreased from 100 to 0% over 56 days, with reduced radicle and hypocotyl lengths, fewer normal seedlings, and more abnormal or dead seeds. Peroxide values rose significantly, from 1.81 to 6.50 meq.kg^-1 (WN) and 0.85 to 3.22 meq.kg^-1 (MN), while free fatty acids increased from 0.41 to 2.95% oleic (WN) and 0.40 to 3.18% oleic (MN). Tocopherol content decreased markedly, disrupting the antioxidant-prooxidant balance. These biochemical changes resulted in higher saturated fatty acids, reducing membrane fluidity, and increasing electrical conductivity from 129.26 to 399.25 μS.cm^-1.g^-1 (WN) and 177.06 to 500.81 μS.cm^-1.g^-1 (MN). Thermal properties analyzed by DSC highlighted transitions within -90 to 100 °C, while FTIR spectroscopy revealed viability-related changes, particularly in the 1740 cm^-1 region. These findings underscore the impact of oxidative stress on seed quality, posing challenges for conservation and commercialization and emphasizing the need for strategies to mitigate storage-related deterioration.</p><p><strong>Graphic abstract: </strong></p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 4","pages":"623-640"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116978/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular underpinnings of <i>EbMYBP1</i>-mediated plant defense against UV-B radiation.","authors":"Chunfan Xiang, Juan Wang, Pinhan Zhou, Mamtimin Mamat, Eparay Abdisattar, Lesong Li, Yan Zhao","doi":"10.1007/s12298-025-01598-y","DOIUrl":"10.1007/s12298-025-01598-y","url":null,"abstract":"<p><p>MYB transcription factors play an important role in the response of plants to abiotic stress<b>.</b> The flavonoids found in <i>Erigeron breviscapus</i> have significant anti-inflammatory and cardiovascular therapeutic effects. It has been discovered that <i>EbMYBP1</i>, a gene cloned from <i>E.breviscapus</i>, positively regulates flavonoid synthesis. However, it is uncertain whether <i>EbMYBP1</i>-OE directly responds to ultraviolet B (UV-B) by increasing flavonoids accumulation. Here, an integrated metabolome-transcriptome analysis revealed an important role for <i>EbMYBP1</i> in transgenic tobacco seeds in response to UV-B. The role of <i>EbMYBP1</i> under UV-B has been examined. The results showed that a higher level of UV-B tolerance was observed in seedlings and leaves of <i>EbMYBP1</i>-OE lines (OE8, OE10, OE15) than in wild-type line (WT), identifying several flavonoid biosynthesis genes and metabolites. Compared with WT, a significant decrease in reactive oxygen species (ROS), an increase in antioxidant enzyme expression, and significant induction of genes involved in flavonoids synthesis, UV-B response, and ROS was observed after UV-B treatment in <i>EbMYBP1</i>-OE lines. Overall, <i>EbMYBP1</i> modulates ROS scavengers and upregulates stress response genes to increase UV-B tolerance.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01598-y.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 4","pages":"609-622"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144182299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analyses reveal <i>TaWRKY41</i> as a potential candidate governing spot blotch resistance in wheat.","authors":"Bhakti R Dayama, Varsha A Mahadik, Deepika Somani, Balkrishna A Shinde, Kirtikumar R Kondhare, Muthukumarasamy Karthikeyan, Narendra Y Kadoo","doi":"10.1007/s12298-025-01583-5","DOIUrl":"10.1007/s12298-025-01583-5","url":null,"abstract":"<p><p>Spot blotch disease caused by <i>Bipolaris sorokiniana</i> poses a significant threat to wheat production. Cultivation of disease-resistant wheat genotypes appears to be the most practical approach to mitigate the impact of this devastating disease. However, the molecular responses of wheat plants during spot blotch disease progression remain poorly understood. This study employed RNA-sequencing to unravel the spatiotemporal molecular events underlying the resistance mechanism in the spot blotch susceptible and resistant wheat genotypes. This study further provides a comprehensive overview of differentially expressed transcripts through functional analysis and transcription factor identification, elucidating the biological mechanisms governing wheat-<i>B. sorokiniana</i> interaction. In the resistant genotype, the expression of one of the key transcription factors, <i>TaWRKY41</i>, was significantly induced upon pathogen inoculation. Computational studies, electrophoretic-mobility shift assay, and yeast one-hybrid assay confirmed the interaction of the recombinant TaWRKY41 protein with W-box elements present in the promoters of plant defense-related genes. Furthermore, co-expression network analyses identified downstream genes positively correlated with <i>TaWRKY41</i>, providing insights into their probable involvement in the defense response. Overall, our investigation suggests that <i>TaWRKY41</i> contributes to spot blotch resistance in wheat. This knowledge can help develop new disease-resistant wheat varieties.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01583-5.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 4","pages":"591-608"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116962/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insight into polyethylene glycol-mediated physiochemical, nutritional, and antioxidative defense modulations in salt-stressed <i>Raphanus sativus</i> L.","authors":"Muhammad Sajid, Shakil Ahmed, Rehana Sardar, Nasim Ahmad Yasin","doi":"10.1007/s12298-025-01585-3","DOIUrl":"10.1007/s12298-025-01585-3","url":null,"abstract":"<p><p>Salinity is one of the most crucial factors that impede various morphological and physiological parameters, eventually reducing crop production. Chemical and physical weathering, in addition to poor irrigation practices, enhances soil salinity. Radish (<i>Raphanus sativus</i> L<i>.</i>), a leafy and root vegetable, is cultivated worldwide because of its nutritional value. However, salinity poses a serious threat to its productivity. Polyethylene glycol (PEG) is mainly used to induce and study osmotic stress in plants. However, our novel research work was designed to observe the stress-mitigating potential of PEG (10%, 20%, 30%, and 40% PEG) in <i>R. sativus</i> subjected to salinity stress (200 mM NaCl). Salt toxicity significantly reduced the seed germination (61.03%), seedling vigor index (54.25%), total soluble protein (69.23%), and biomass accumulation (42.25%) of <i>R. sativus</i> plants. Similarly, stressed plants presented a reduced synthesis of photosynthetic pigments and poor nutrition. However, seed priming with PEG-30% significantly alleviated salt stress by promoting growth attributes, mineral uptake, and the antioxidative defence system of <i>R. sativus</i> under salinity regimes. Plants raised from seeds treated with 30% PEG alleviated NaCl-induced oxidative stress by modulating the activity of antioxidative enzymes such as peroxidase, ascorbate peroxidase, glutathione peroxidase, glutathione S-transferase, ascorbic acid, superoxide dismutase, and catalase. Furthermore, PEG-30% significantly improved photosynthetic pigment biosynthesis, although there was a decrease in electrolyte leakage and lipid peroxidation in plants under saline conditions. Furthermore, 30% PEG improved the shoot length (41.46%), root length (46.57%), and biomass production (53.93%) of salt-stressed plants. This study revealed that 30% PEG is beneficial for reversing salt stress. However, extensive field studies are required to assess the potential of PEG for mitigating salt stress in various geographical regions.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01585-3.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 4","pages":"659-674"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116968/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144183695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon assimilation dynamics in <i>Pontederia crassipes</i> in response to light intensity and CO₂ levels.","authors":"Bianca Jaqueline Santos Rodrigues, Marcos Antonio Bacarin, Junior Borella","doi":"10.1007/s12298-025-01587-1","DOIUrl":"10.1007/s12298-025-01587-1","url":null,"abstract":"<p><p><i>Pontederia crassipes</i> Mart. is an aquatic macrophyte native to South America, tolerant to high intensity of sunlight and has spread to various countries worldwide. This study aimed to investigate the photosynthetic characteristics of <i>P. crassipes</i> leaves under different light intensities and CO₂ concentrations by analyzing net photosynthetic response, Rubisco activity, and related photosynthetic parameters. The plants were acclimated in a greenhouse under natural light conditions at a temperature of 25 ± 5 °C. The light response curve was measured using a portable infrared gas exchange system, coupled with the 6400-40 Leaf Chamber Fluorometer. The response of net photosynthesis to intercellular CO₂ concentration was determined at 1500 µmol m^-2 s^-1 of photosynthetic active radiation (PAR). <i>P. crassipes</i> demonstrated a remarkable capacity for adaptation to varying light intensities and CO₂ concentrations, exhibiting strong photosynthetic efficiency, as indicated by its net CO₂ assimilation rate in response to PAR, sustained electron transport rate up to 1000 µmol photons m^-2 s^-1, a positive correlation between φPSII and φCO₂, and a high net CO₂ assimilation rate in response to <i>C</i> _<i>i</i> .</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 4","pages":"641-645"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116967/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144182093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}