Physiology and Molecular Biology of Plants最新文献

{"title":"Biotechnological interventions for the production of forskolin, an active compound from the medicinal plant, Coleus forskohlii","authors":"Pulukkunadu Thekkeveedu Roshni, Punchappady Devasya Rekha","doi":"10.1007/s12298-024-01426-9","DOIUrl":"https://doi.org/10.1007/s12298-024-01426-9","url":null,"abstract":"<p><i>Coleus forskohlii</i>, an Indian-origin medicinal plant is the sole natural source of the labdane terpenoid forskolin (C₂₂H₃₄O₇), with growing demand<i>.</i> Forskolin emerged as an industrially important bioactive compound, with many therapeutic applications in human health. It has established potential effects in the treatment of various diseases and conditions such as glaucoma, asthma, obesity, allergies, skin conditions and cardiovascular diseases. Moreover, clinical trials against different types of cancers are progressing. The mechanism of action of forskolin mainly involves activating adenylyl cyclase and elevating cAMP, thereby regulating different cellular processes. For the extraction of forskolin, tuberous roots of <i>C. forskohlii</i> are used as they contain the highest concentration of this metabolite. Approximately 2500 tonnes of the plant are cultivated annually to produce a yield of 2000–2200 kg ha⁻¹ of dry tubers. The forskolin content of the root is distributed in the range of 0.01–1%, which cannot meet the increasing commercial demands from industries such as pharmaceuticals, cosmetics, dietary supplements, food and beverages. Hence, various aspects of micropropagation with different culture methods that employ precursors or elicitors to improve the forskolin content have been explored. Different extraction and analytical methods are also introduced to examine the yield and purity of forskolin. This review discusses the significance, clinical importance, mechanism of action and different approaches used for mass production including tissue culture for the lead compound forskolin to meet market needs.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"44 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140127305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of chloroplast biogenesis, development, and signaling by endogenous and exogenous cues","authors":"","doi":"10.1007/s12298-024-01427-8","DOIUrl":"https://doi.org/10.1007/s12298-024-01427-8","url":null,"abstract":"<h3>Abstract</h3> <p>Chloroplasts are one of the defining features in most plants, primarily known for their unique property to carry out photosynthesis. Besides this, chloroplasts are also associated with hormone and metabolite productions. For this, biogenesis and development of chloroplast are required to be synchronized with the seedling growth to corroborate the maximum rate of photosynthesis following the emergence of seedlings. Chloroplast biogenesis and development are dependent on the signaling to and from the chloroplast, which are in turn regulated by several endogenous and exogenous cues. Light and hormones play a crucial role in chloroplast maturation and development. Chloroplast signaling involves a coordinated two-way connection between the chloroplast and nucleus, termed retrograde and anterograde signaling, respectively. Anterograde and retrograde signaling are involved in regulation at the transcriptional level and downstream modifications and are modulated by several metabolic and external cues. The communication between chloroplast and nucleus is essential for plants to develop strategies to cope with various stresses including high light or high heat. In this review, we have summarized several aspects of chloroplast development and its regulation through the interplay of various external and internal factors. We have also discussed the involvement of chloroplasts as sensors of various external environment stress factors including high light and temperature, and communicate via a series of retrograde signals to the nucleus, thus playing an essential role in plants’ abiotic stress response.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"10 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140099546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological response and tolerance of Sesuvium portulacastrum L. to low temperature stress","authors":"Jingtao Ye, Jingyi Yang, Rou Zheng, Jiawen Yu, Xiamin Jiang, Sheng Li, Maowang Jiang","doi":"10.1007/s12298-024-01429-6","DOIUrl":"https://doi.org/10.1007/s12298-024-01429-6","url":null,"abstract":"<p>The plant <i>Sesuvium portulacastrum</i> L., commonly referred to as sea purslane, is a perennial halophytic species with significant potential for development in marine ecological restoration. However, its growth is limited in high-latitude regions with lower temperatures due to its subtropical nature. Furthermore, literature on its cold tolerance is scarce. This study, therefore, focused on sea purslane plants naturally overwintering in Ningbo (29°77’N), investigating their morphological, histological, rooting, and physiological responses to low temperatures (7 °C, 11 °C, 15 °C, and 19 °C). The findings indicated an escalation in cold damage severity with decreasing temperatures. At 7 °C, the plants failed to root and subsequently perished. In contrast, at 11 °C, root systems developed, while at 15 °C and 19 °C, the plants exhibited robust growth, outperforming the 11 °C group in terms of leaf number and root length significantly (<i>P</i> &lt; 0.05). Histological analyses showed a marked reduction in leaf thickness under cold stress (<i>P</i> &lt; 0.05), with disorganized leaf structure observed in the 7 °C group, whereas it remained stable at higher temperatures. No root primordia were evident in the vascular cambium of the 7 and 11 °C groups, in contrast to the 15 and 19 °C groups. Total chlorophyll content decreased with temperature, following the order: 19 °C &gt; 15 °C &gt; 11 °C &gt; 7 °C. Notably, ascorbic acid levels were significantly higher in the 7 and 11 °C groups than in the 15 and 19 °C groups. Additionally, the proline concentration in the 7 °C group was approximately fourfold higher than in the 19 °C group. Activities of antioxidant enzymes—superoxide dismutase, peroxidase, and catalase—were significantly elevated in the 7 and 11 °C groups compared to the 15 and 19 °C groups. Moreover, the malondialdehyde content in the 7 °C group (36.63 ± 1.75 nmol/g) was significantly higher, about 5.5 and 9.6 times, compared to the 15 °C and 19 °C groups, respectively. In summary, 7 °C is a critical threshold for sea purslane stem segments; below this temperature, cellular homeostasis is disrupted, leading to an excessive accumulation of lipid peroxides and subsequent death due to an inability to neutralize excess reactive oxygen species. At 11 °C, although photosynthesis is impaired, self-protective mechanisms such as enhanced antioxidative systems and osmoregulation are activated. However, root development is compromised, resulting in stunted growth. These results contribute to expanding the geographic distribution of sea purslane and provide a theoretical basis for its ecological restoration in high-latitude mariculture.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"26 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140099671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of codon usage patterns in the chloroplast genomes of nine forage legumes","authors":"Mingkun Xiao, Xiang Hu, Yaqi Li, Qian Liu, Shaobin Shen, Tailing Jiang, Linhui Zhang, Yingchun Zhou, Yuexian Li, Xin Luo, Lina Bai, Wei Yan","doi":"10.1007/s12298-024-01421-0","DOIUrl":"https://doi.org/10.1007/s12298-024-01421-0","url":null,"abstract":"<p><i>Leguminosae</i> is one of the three largest families of angiosperms after <i>Compositae</i> and <i>Orchidaceae</i>. It is widely distributed and grows in a variety of environments, including plains, mountains, deserts, forests, grasslands, and even waters where almost all legumes can be found. It is one of the most important sources of starch, protein and oil in the food of mankind and also an important source of high-quality forage material for animals, which has important economic significance. In our study, the codon usage patterns and variation sources of the chloroplast genome of nine important forage legumes were systematically analyzed. Meanwhile, we also constructed a phylogenetic tree based on the whole chloroplast genomes and protein coding sequences of these nine forage legumes. Our results showed that the chloroplast genomes of nine forage legumes end with A/T bases, and seven identical high-frequency (HF) codons were detected among the nine forage legumes. ENC-GC3s mapping, PR2 analysis, and neutral analysis showed that the codon bias of nine forage legumes was influenced by many factors, among which natural selection was the main influencing factor. The codon usage frequency showed that the <i>Nicotiana tabacum</i> and <i>Saccharomyces cerevisiae</i> can be considered as receptors for the exogenous expression of chloroplast genes of these nine forage legumes. The phylogenetic relationships of the chloroplast genomes and protein coding genes were highly similar, and the nine forage legumes were divided into three major clades. Among the clades <i>Melilotus officinalis</i> was more closely related to <i>Medicago sativa</i>, and <i>Galega officinalis</i> was more closely related to <i>Galega orientalis</i>. This study provides a scientific basis for the molecular markers research, species identification and phylogenetic studies of forage legumes.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"12 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140099548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of bHLH transcription factors and expression analysis under drought stress in Pseudoroegneria libanotica at germination","authors":"Xingguan Zhai, Xia Wang, Xunzhe Yang, Qingxiang Huang, Dandan Wu, Yi Wang, Houyang Kang, Lina Sha, Xing Fan, Yonghong Zhou, Haiqin Zhang","doi":"10.1007/s12298-024-01433-w","DOIUrl":"https://doi.org/10.1007/s12298-024-01433-w","url":null,"abstract":"<p>The basic helix-loop-helix (bHLH) transcription factor family is the second largest in plants. bHLH transcription factor is not only universally involved in plant growth and metabolism, including photomorphogenesis, light signal transduction, and secondary metabolism, but also plays an important role in plant response to stress. However, the function of bHLH TFs in <i>Pseudoroegneria</i> species has not been studied yet. <i>Pseudoroegneria</i> (Nevski) Á. Löve is a perennial genus of the <i>Triticeae</i>. <i>Pseudoroegneria</i> species are mostly distributed in arid/semi-arid areas and they show good drought tolerance. In this study, we identified 152 PlbHLH TFs in <i>Pseudoroegneria libanotica</i>, which could be classified into 15 groups. Collinearity analysis indicates that 122 <i>PlbHLH</i> genes share homology with <i>wbHLH</i> genes in wheat, and it has lower homology with <i>AtbHLH</i> genes in <i>Arabidopsis</i>. Based on transcriptome profiling under an experiment with three PEG concentrations (0%, 10%, and 20%), 10 up-regulated genes and 11 down-regulated <i>PlbHLH</i> genes were screened. Among them, <i>PlbHLH6</i>, <i>PlbHLH55</i> and <i>PlbHLH64</i> as candidate genes may be the key genes related to drought tolerance response at germination, and they have been demonstrated to respond to drought, salt, oxidative, heat, and heavy metal stress in yeast. This study lays the foundation for an in-depth study of the biological roles of <i>PlbHLHs</i> in <i>Pse. libanotica</i>, and discovered new drought-tolerance candidate genes to enhance the genetic background of <i>Triticeae</i> crops.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"284 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140099549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The combined application of rutin and silicon alleviates osmotic stress in maize seedlings by triggering accumulation of osmolytes and antioxidants’ defense mechanisms","authors":"","doi":"10.1007/s12298-024-01430-z","DOIUrl":"https://doi.org/10.1007/s12298-024-01430-z","url":null,"abstract":"<h3>Abstract</h3> <p>Silicon (Si) has been shown to improve plant defenses against a variety of stresses. However, how rutin (Rut) affects stress factors is yet to be fully explored. Moreover, their combined role in osmotic stress response remains unclear. The current study was performed to determine how the use of Rut and Si, both separately and in combination, improved the physiological resilience of maize seedlings to two levels of osmotic stress (induced by polyethylene glycol (PEG) 6000). We aimed to enhance osmotic stress tolerance with the simultaneous use of Rut and Si. First, we selected the best water status and the lowest membrane damage enhancing concentration of Rut (60 ppm) and Si (1 mM) to research their tolerance and resistance to osmotic stress (moderate: 10% PEG, severe: 15% PEG). The application of Rut and Si separately and together reduced oxidative stress by decreasing the reactive oxygen species and improved the relative water content, osmoprotectants (proline, total soluble sugar, and glycine-betaine), ascorbate level, and some antioxidant defense-related enzyme activities and their gene expression in maize seedlings under osmotic stress. However, these effects were more promising under moderate stress. As a result, findings from the study indicate the synergistic effect of combined Rut and Si on osmotic stress tolerance in maize seedlings. Overall, the combination of Rut and Si was more effective than independent Rut and Si in reducing osmotic stress in maize seedlings. Here, it was clear that Rut played an active role in alleviating stress. This combined application can be useful for developing drought tolerance in crops for the agriculture sector.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"88 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140099550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agronomic potential of plant-specific Gγ proteins","authors":"Sona Pandey","doi":"10.1007/s12298-024-01428-7","DOIUrl":"https://doi.org/10.1007/s12298-024-01428-7","url":null,"abstract":"<p>The vascular plant-specific type III Gγ proteins have emerged as important targets for biotechnological applications. These proteins are exemplified by Arabidopsis AGG3, rice Grain Size 3 (GS3), Dense and Erect Panicle 1 (DEP1), and GGC2 and regulate plant stature, seed size, weight and quality, nitrogen use efficiency, and multiple stress responses. These Gγ proteins are an integral component of the plant heterotrimeric G-protein complex and differ from the canonical Gγ proteins due to the presence of a long, cysteine-rich C-terminal region. Most cereal genomes encode three or more of these proteins, which have similar N-terminal Gγ domains but varying lengths of the C-terminal domain. The C-terminal domain is hypothesized to give specificity to the protein function. Intriguingly, many accessions of cultivated cereals have natural deletion of this region in one or more proteins, but the mechanistic details of protein function remain perplexing. Distinct, sometimes contrasting, effects of deletion of the C-terminal region have been reported in different crops or under varying environmental conditions. This review summarizes the known roles of type III Gγ proteins, the possible action mechanisms, and a perspective on what is needed to comprehend their full agronomic potential.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"21 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140099670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional regulation of tomato fruit ripening","authors":"Priya Gambhir, Utkarsh Raghuvanshi, Rahul Kumar, Arun Kumar Sharma","doi":"10.1007/s12298-024-01424-x","DOIUrl":"https://doi.org/10.1007/s12298-024-01424-x","url":null,"abstract":"<p>An intrinsic and genetically determined ripening program of tomato fruits often depends upon the appropriate activation of tissue- and stage-specific transcription factors in space and time. The past two decades have yielded considerable progress in detailing these complex transcriptional as well as hormonal regulatory circuits paramount to fleshy fruit ripening. This non-linear ripening process is strongly controlled by the MADS-box and NOR family of proteins, triggering a transcriptional response associated with the progression of fruit ripening. Deepening insights into the connection between MADS-RIN and plant hormones related transcription factors, such as ERFs and ARFs, further conjugates the idea that several signaling units work in parallel to define an output fruit ripening transcriptome. Besides these TFs, the role of other families of transcription factors such as MYB, GLK, WRKY, GRAS and bHLH have also emerged as important ripening regulators. Other regulators such as EIN and EIL proteins also determine the transcriptional landscape of ripening fruits. Despite the abundant knowledge of the complex spectrum of ripening networks in the scientific domain, identifying more ripening effectors would pave the way for a better understanding of fleshy fruit ripening at the molecular level. This review provides an update on the transcriptional regulators of tomato fruit ripening.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"35 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140099673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ALLENE OXIDE SYNTHASE (AOS) induces petal senescence through a novel JA-associated regulatory pathway in Arabidopsis","authors":"Liuqing Wu, Kaiqi Wang, Mengyi Chen, Wenxin Su, Zheng Liu, Xiaoying Guo, Mengqian Ma, Shuangjie Qian, Yuqi Deng, Haihan Wang, Chanjuan Mao, Zaibao Zhang, Xiaofeng Xu","doi":"10.1007/s12298-024-01425-w","DOIUrl":"https://doi.org/10.1007/s12298-024-01425-w","url":null,"abstract":"<p>Flowers are crucial for the reproduction of flowering plants and their senescence has drastic effects on plant-animal interactions as well as pollination. Petal senescence is the final phase of flower development which is regulated by hormones and genes. Among these, jasmonic acid (JA) has emerged as a major contributor to petal senescence, but its molecular mechanisms remain elusive. Here, the role of JA in petal senescence in <i>Arabidopsis</i> was investigated. We showed that petal senescence in <i>aos</i> mutant was significantly delayed, which also affected petal cell size and proliferation. Similar significant delays in petal senescence were observed in <i>dad1</i> and <i>coi1</i> mutants. However, <i>MYB21/24</i> and <i>MYC2/3/4</i>, known downstream regulators of JA in flower development, played no role in petal senescence. This indicated that JA regulates petal senescence by modulating other unknown transcription factors. Transcriptomic analysis revealed that <i>AOS</i> altered the expression of 3681 genes associated, and identified groups of differentially expressed transcription factors, highlighting the potential involvement of AP-2, WRKY and NAC. Furthermore, <i>bHLH13</i>, <i>bHLH17</i> and <i>URH2</i> were identified as potential new regulators of JA-mediated petal senescence. In conclusion, our findings suggest a novel genetic pathway through which JA regulates petal senescence in <i>Arabidopsis</i>. This pathway operates independently of stamen development and leaf senescence, suggesting the evolution of specialized mechanisms for petal senescence.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"298 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140075433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revisiting plant stress memory: mechanisms and contribution to stress adaptation","authors":"Abu Bakar Siddique, Sumaya Parveen, Md. Zahidur Rahman, Jamilur Rahman","doi":"10.1007/s12298-024-01422-z","DOIUrl":"https://doi.org/10.1007/s12298-024-01422-z","url":null,"abstract":"<p>Highly repetitive adverse environmental conditions are encountered by plants multiple times during their lifecycle. These repetitive encounters with stresses provide plants an opportunity to remember and recall the experiences of past stress-associated responses, resulting in better adaptation towards those stresses. In general, this phenomenon is known as plant stress memory. According to our current understanding, epigenetic mechanisms play a major role in plants stress memory through DNA methylation, histone, and chromatin remodeling, and modulating non-coding RNAs. In addition, transcriptional, hormonal, and metabolic-based regulations of stress memory establishment also exist for various biotic and abiotic stresses. Plant memory can also be generated by priming the plants using various stressors that improve plants’ tolerance towards unfavorable conditions. Additionally, the application of priming agents has been demonstrated to successfully establish stress memory. However, the interconnection of all aspects of the underlying mechanisms of plant stress memory is not yet fully understood, which limits their proper utilization to improve the stress adaptations in plants. This review summarizes the recent understanding of plant stress memory and its potential applications in improving plant tolerance towards biotic and abiotic stresses.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"8 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140075361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}