Physiology and Molecular Biology of Plants最新文献

{"title":"Constitutive expression of <i>CEN-like protein 2</i>, a <i>TFL1</i> ortholog of pigeon pea (<i>Cajanus cajan</i> [L.] Millspaugh) delays flowering in transgenic tobacco plants.","authors":"Sougata Bhattacharjee, Krishnayan Paul, K Venkat Raman, Jyotsana Tilgam, Priyanka Kumari, Mahi Baaniya, Rohini Sreevathsa, Anjali Anand, G Rama Prashat, Debasis Pattanayak","doi":"10.1007/s12298-025-01572-8","DOIUrl":"10.1007/s12298-025-01572-8","url":null,"abstract":"<p><p><i>CEN-like protein 2</i> of pigeon pea, a candidate anti-florigen gene and a close homolog of <i>AtTFL1</i> (<i>Arabidopsis Terminal Flower1</i>) of the PEBP family has been characterized through constitutive expression in tobacco. <i>In-silico</i> analysis helped to demonstrate the absence of a nuclear binding domain and the conserveness of substrate binding sites of this protein across angiosperms. Transgenic tobacco lines with 2-eightfold higher expressions of <i>CEN-like protein 2</i> showed delayed flowering (26-32 days) along with significant morphological changes, including vegetative vigour, number and size of flowers, fruit setting, etc. Together, these findings showed that <i>CEN-like protein 2</i> not only delays floral transition through repression but also regulates a variety of developmental traits. Expression profiling of upstream and downstream interacting pathway genes explained that their expression modulation led to a prolonged vegetative phase of over-expressed lines. Floral inducer genes like <i>APETALA1</i> and <i>LEAFY</i> were drastically down-regulated in transgenic lines, reconfirming the role of the <i>CEN-like 2</i> gene in floral regulation. In conclusion, precisely controlling <i>CcCEN-like 2</i> gene expression may prove useful for refining pigeon pea breeding.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01572-8.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 3","pages":"419-433"},"PeriodicalIF":3.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of cadmium (Cd) on photosynthetic characteristics and chlorophyll fluorescence parameters in the ornamental Plant <i>Salvia splendens</i> Ker-Gawl.","authors":"Lian Jia, Guangchao Yu, Zhen Zhao, LinLin Lü","doi":"10.1007/s12298-025-01584-4","DOIUrl":"10.1007/s12298-025-01584-4","url":null,"abstract":"<p><p><i>Salvia splendens</i> Ker-Gawl. (scarlet sage), widely used in urban landscaping, it is frequently exposed to cadmium (Cd) contamination resulting from industrial and vehicular emissions. However, its tolerance and adaptability to Cd stress remain poorly understood. A soil experiment was conducted to investigate the effects of Cd on the growth and the photosynthetic performance of <i>S. splendens</i> by measuring photosynthetic pigments, gas exchange and chlorophyll fluorescence parameters. Four weeks-seedlings were treated with 0 (CK), 0.5, 2.5, 5, 10, 25 and 50 mg·kg^-1 Cd for 60 days. Results showed significant reductions in root length and biomass of leaves, stems, and roots, with shoot and root biomass notably decreasing by up to 46.3% and 28.5% at higher Cd levels, respectively. The translocation factor remained low (TF < 1.0), and the bioaccumulation factors (BCF < 1.0) decreased when Cd higher than 5 mg·kg^-1, indicating limited Cd uptake. Cd stress (> 5 mg·kg^-1) caused a decrease in Chl a and Chl b content, but increased the Chl a/b ratio, thereby disrupting photosynthesis and causing significant declines in photosynthetic parameters. Cd exposure (> 2.5 mg·kg^-1) significantly decreased net photosynthetic rate (Pn) by 18.94-52.91%, stomatal conductance (Gs) by 35.77-58.53%, and transpiration rate (Tr) by 24.63-48.83%, accompanied by only a slight reduction in inter-cellular CO₂ concentration (Ci) of just 7.0%, indicating non-stomatal factors in Pn decline. Cd concentrations (> 5 mg·kg^-1) caused a reduction in initial fluorescence (Fo) by 7.44-31.58% and maximal fluorescence (Fm) measurements by about 20%, indicating damage to photosystem II (PSII). At 50 mg·kg^-1, further decreases were observed in photochemical quenching (qP) by 40.31%, the quantum yield of photochemical energy dissipation (ΦPSII) by 44.77%, and the electron transport rate (ETR) by 25.11%, while non-photochemical quenching increased by 42.66%, signifying significant PSII inhibition and enhanced photoinhibition. Decrease in ΦPSII, along with the increase in the quantum yield of regulated non-photochemical energy loss in PSII (ΦNPQ) and the quantum yield of non-regulated energy loss in PSII (ΦNO) as Cd levels rise, indicates enhanced non-photochemical energy dissipation and greater photoinhibition. <i>S. splendens</i> shows high sensitivity to Cd stress, with reduced growth and disrupted photosynthesis, highlighting its potential as a bioindicator for Cd contamination in urban areas.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 3","pages":"507-519"},"PeriodicalIF":3.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional identification of <i>JrMYBs</i> gene in Walnut (<i>Juglans regia</i>) demonstrates it enhances drought stress tolerance.","authors":"Yanglong Gao, Fange Gong, Xiaolan Ma, Zhongxing Zhang, Yanxiu Wang","doi":"10.1007/s12298-025-01568-4","DOIUrl":"10.1007/s12298-025-01568-4","url":null,"abstract":"<p><p>Walnut is a popular nut tree species and often undergoes drought stress. The MYB transcription factor plays a crucial role in a series of plant biological processes and is extensively involved in plant metabolism, growth, and development, as well as biological and abiotic stress. However, there is little information on the response mechanisms of Walnuts to drought stress, resulting in a lack of basic understanding of their drought resistance. In order to explore more functional genes that can respond to stress and enrich the theoretical basis of walnut stress resistance, two MYB transcription factors (<i>JrMYB48</i> and <i>JrMYB62</i>) were identified and their functions were preliminarily investigated. Bioinformatics analysis showed that <i>JrMYB48</i> and <i>JrMYB62</i> are both unstable hydrophobic proteins, and their promoter contain various stress and hormone response elements. qRT-PCR analysis showed that <i>JrMYB48</i> and <i>JrMYB62</i> genes could be significantly induced by drought stress. Compared to Wild type (WT), overexpression of <i>JrMYB48</i> and <i>JrMYB62</i> not only increased germination rate of <i>Arabidopsis thaliana</i> treated with mannitol, the activity of antioxidant enzymes and the content of osmoregulatory substances under drought stress, but also decreased the water loss rate of <i>Arabidopsis</i>, and also induced the up-regulated expression of drought and ABA-related genes, thus improving the drought tolerance of <i>Arabidopsis</i>, which will provide a theoretical basis for further digging drought response functional genes in walnut.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01568-4.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 3","pages":"375-387"},"PeriodicalIF":3.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006606/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144040597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics analysis of non-pungent (<i>Capsicum annuum</i>) and fiery hot ghost chili (<i>C. chinense</i>) provides insights into proteins involved in fruit development and metabolites biosynthesis.","authors":"John Momo, Khushbu Islam, Souparna Biswas, Abdul Rawoof, Ilyas Ahmad, Vishesh, Nirala Ramchiary","doi":"10.1007/s12298-025-01581-7","DOIUrl":"10.1007/s12298-025-01581-7","url":null,"abstract":"<p><p>Global omics offer extensive insights into the diversity of essential biomolecules across various plant developmental stages. Despite advancements in high-throughput technologies, the integrated analysis of global omics such as proteomics, transcriptomics, and metabolomics, is yet to be fully explored in fruits of <i>Capsicum</i> species. In this study, we used an integrated omics approach to identify proteins involved in fruit development, and metabolite biosynthesis in the placenta and pericarp tissues of two contrasting genotypes belonging to ghost chili (<i>Capsicum chinense</i>) and <i>C. annuum</i>. The mass spectrometry analysis identified a total of 4,473 and 2,012 proteins from the pericarp and placenta tissues of <i>Capsicum</i> fruits. We observed expression of developmental stage-specific proteins, such as kinases, transferases, ion transporters, F-box proteins, and transcription factors that were enriched in the biosynthesis of primary and secondary metabolites. The abundance of these proteins corresponded with RNAseq data. Key proteins related to capsaicinoids biosynthesis, such as Acyltransferase 3, 3-oxoacyl-[acyl-carrier protein], 4-coumaroyl co-A ligase, and 3-ketoacyl-coA synthase 3, were identified in placenta of highly pungent ghost chili, along with J-domain proteins and transcription factors such as MYB101, MYB 14-like, bHLH112, NAC, and Cyt p450 CYP82D47, suggesting their role in capsaicinoids and secondary metabolites biosynthesis. Further, we observed a correlation of the expression of genes and proteins with the abundance of primary and secondary metabolites, such as carbohydrates, alcohols, fatty acids, phenolics, glycerides, polyamines, and amino acids. Our findings provide a novel multiomics resources for future functional studies, with potential applications in breeding programs.</p><p><strong>Graphical abstract: </strong></p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01581-7.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 3","pages":"453-475"},"PeriodicalIF":3.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide association analysis in identification of superior haplotypes for vegetative stage drought stress tolerance in rice.","authors":"Gyanisha Nayak, C Parameswaran, Nairita Vaidya, Madhuchhanda Parida, S Sabarinathan, Prabharani Chaudhari, Pallavi Sinha, Vikas Kumar Singh, Sanghamitra Samantaray, Jawahar Lal Katara","doi":"10.1007/s12298-025-01573-7","DOIUrl":"10.1007/s12298-025-01573-7","url":null,"abstract":"<p><p>Water availability is the most critical factor limiting rice yield in rainfed agro-ecosystems. Drought stress during the vegetative stage inhibits key growth processes, such as leaf formation and tillering, significantly impacting yield. This study aimed to investigate the genetic basis of vegetative stage drought tolerance and identify QTLs and genes associated with it through GWAS. A total of 19 major QTLs were identified for six traits: leaf rolling, relative water content, plant height, leaf area, tiller number, and leaf number, with phenotypic variances ranging from 10.55 to 80.05%. Additionally, haplotypes for six candidate genes were identified: <i>OsCYP72A32</i> for leaf rolling, <i>OsNCX5.2</i> for relative water content, <i>OsSPX2</i> for plant height, <i>OsSTA104</i> for tiller number, <i>OsRING313</i> for leaf number and <i>Os3BGlu6</i> for leaf area.  Besides, genotypes such as NCS 901 A, H 15-23-DA, LOHAMBITRO and MEJANES 2 were found to be superior donors. These tolerant genotypes and superior haplotypes can be used in haplotype-based breeding programs to enhance drought tolerance in rice at vegetative stage.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01573-7.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 3","pages":"435-452"},"PeriodicalIF":3.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006632/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144046764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and biochemical response of mixed lupine and barley cultures under changing environmental conditions during spring.","authors":"Marko Kolaksazov, Ivanina Vasileva, Ina Stoycheva","doi":"10.1007/s12298-025-01577-3","DOIUrl":"10.1007/s12298-025-01577-3","url":null,"abstract":"<p><p>Mixed cultivation of grass-legume forage crops, such as lupine (<i>Lupinus albus</i> L.) and barley (<i>Hordeum vulgare</i> L.), offers significant advantages in terms of nitrogen utilization, stress resistance and a balanced diet for ruminants. This study explored the symbiotic effects of these crops on photosynthesis and stress tolerance via measuring key physiological and biochemical parameters. Measurements were performed on the photosynthetic activity, chlorophyll and carotenoid content, glycolate oxidase activity, antioxidant capacity, and total phenolic content. The varying temperatures during May, allowed the effects of mixed cultivation on the response to chilling to be analyzed. Notably, barley monoculture was the most affected by the decreased temperatures. In general, mixed culture showed mitigation of the effects from chilling, as compared with both lupine and barley monocultures alone. These results suggest an adaptive synergy between lupine and barley, highlighting the potential advantages of mixed cultivation for improving stress tolerance and overall crop performance.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 3","pages":"493-505"},"PeriodicalIF":3.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144049116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolite profiling reveals differential accumulation of secondary metabolites related to flavour and colour across four heirloom chilli landraces.","authors":"Aheibam Loyanganba Meitei, Kalenahalli Yogendra, Hemalatha Sanivarapu, Ngasepam Tombisana Meetei, Mayank Rai, Wricha Tyagi","doi":"10.1007/s12298-025-01576-4","DOIUrl":"10.1007/s12298-025-01576-4","url":null,"abstract":"<p><p>Chillies from Northeast India exhibit wide variability in fruit morphology, pungency, bearing habit and crop duration. An untargeted metabolite profiling using LC-HRMS of four 'heirloom' pungent landraces viz. Naga chilli (AL-1), Dalle khursani (AL-2), Sohmynken khnai (AL-3), and J-41(B) was performed and compared with Kashi anmol (KA). While AL-2, J-41(B) and KA belong to <i>C. annuum</i> species, AL-1 is categorised as <i>C. chinense</i> and AL-3 is <i>C. frutescens</i>. A total of 6990 consistent peaks of monoisotopic masses were detected, out of which 2702 metabolites were identified using accurate mass error < 10 ppm. A higher number of differentially accumulated metabolites were seen in J-41(B) versus AL-3 (1376), followed by J-41(B) versus AL-2 (1365), J-41(B) versus AL-1 (1257), KA versus AL-2 (649), AL-3 versus KA (616), KA versus AL-1 (594) and J-41(B) versus KA (413). Variation among species was higher than variation within species. Pathway analysis identified fatty acid, carotenoid, flavonoid and capsaicinoid as key pathways. We identified eight major categories of metabolites, including fatty acids, sterol lipids, and flavonoids, which together account for over 70% of the significantly expressed metabolites across the genotypes. This study explores untargeted metabolites in various chilli species, offering insights into the biochemical and molecular mechanisms which may play a role in governing important fruit traits. Identification of key metabolites and underlying alleles for twenty-one genes across three pathways (flavonoid, capsaicinoid and carotenoid) suggests that the metabolites and associated alleles identified in this study can be used as biomarkers for further characterization of these heirloom chilli and could provide distinct parameter(s) in distinguishing improved cultivars from landraces. This will contribute towards breeding programs in aiding selection of fruits of the desirable traits.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01576-4.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 3","pages":"477-491"},"PeriodicalIF":3.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144023931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative translational reprogramming of <i>Glycine max</i> during mechanical wounding.","authors":"Manisha Yadav, Megha Kumari, Indrakant Kumar Singh, Archana Singh","doi":"10.1007/s12298-025-01562-w","DOIUrl":"10.1007/s12298-025-01562-w","url":null,"abstract":"<p><p><i>Glycine max</i> (soybean) is a highly protein-rich legume that also contains oils and vitamins. Unfortunately, soybean faces many biotic and abiotic stresses including heat, drought, pests, wounds, infections, and salinity, which limits the crop productivity. Among these, mechanical wounding (MW) causes significant harm to plants, creates a passage for invading pathogens, and disrupts plant metabolism. Thus, exploring soybean responses at the molecular and biochemical levels during mechanical damage is essential. Additionally, MW resembles insect bites, which offers important insights into the immune systems identical to MW and pest attacks. In this investigation, we executed a comparative proteome evaluation of the PUSA9712 soybean variety following MW. Based on specifications of log2FC ≥ 1 and <i>p</i>-value ≤ 0.05, the study disclosed 786 differentially abundant proteins (DAPs) upon MW, among which 294 were elevated and 492 were down-regulated. The function annotation and pathway analysis of DAPs displayed their role in ROS signaling, flavonoid biosynthesis, ABA synthesis, JA-synthesis, defense against pathogens, fatty acid synthesis, brassinosteroid (BR) signaling, carbohydrate metabolism, proteolysis, calcium signaling, and protein kinase pathway. Lipoxygenase, V-type ATPases, Annexin, NsLTP, and ATP-dependent Clp protease proteolytic subunit DAPs can be promising candidates to strengthen soybean crop's resilience to mechanical damage and pest/pathogen attacks and need further functional characterisation.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01562-w.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 2","pages":"263-282"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11890806/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification and expression analysis of <i>CYP450</i>s under various stress treatment in <i>Dendrobium huoshanense</i>.","authors":"Guohui Li, Xingen Zhang, Yuyue Li, Xilu Zhang, Muhammad Aamir Manzoor, Chuanbo Sun, Cheng Song, Min Zhang","doi":"10.1007/s12298-025-01555-9","DOIUrl":"10.1007/s12298-025-01555-9","url":null,"abstract":"<p><p>The cytochrome P450 monooxygenases (CYP450) are the largest enzyme family in plant metabolism, playing a key role in the biosynthesis of both primary and secondary metabolites. However, the CYP450 has not yet been systematically characterized in Dendrobium species. In this study, 193 <i>DhCYP450</i> genes were identified in the genome of <i>Dendrobium huoshanense</i> through bioinformatics, and divided into 2 groups and 10 clans. Chromosome localization results revealed that <i>DhCYP450</i> genes are distributed across 19 chromosomes. We identified eight common conserved motifs within the <i>DhCYP450</i> family of <i>D. huoshanense</i>. Furthermore, prediction analysis of <i>cis</i>-acting elements in the promoter region indicated the presence of elements responsive to low temperature, drought, and hormones responsive elements in most <i>DhCYP450</i> genomes. Quantitative real-time PCR (qRT-PCR) analysis demonstrated the experiments expression patterns of <i>DhCYP450</i> genes in response to cold, drought treatment, and hormones, suggesting their involvement in abiotic stress responses and their role in Dendrobium growth. Overall, these results provide valuable insights into the functional dynamics of the <i>DhCYP450</i> genes and highlight potential candidates for further study of their biological roles.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01555-9.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 2","pages":"311-328"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11890713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roles of methyl-CpG-binding-domain (MBD) protiens in regulation of biological processes in plants.","authors":"Sanskriti Ravi, Amrapali Sharma, Arun Kumar Sharma","doi":"10.1007/s12298-025-01556-8","DOIUrl":"10.1007/s12298-025-01556-8","url":null,"abstract":"<p><p>DNA methylation is a key epigenetic mark found in both eukaryotic as well as prokaryotic genomes. It is essential for regulating expression of genes and preservation of genomic integrity. Both plants and animals possess specific proteins that mediate biological effects of DNA methylation. Many families of such methylated DNA binding proteins have been discovered in plants; the most prominent of them being the methyl-CpG- binding domain (MBD) proteins. Some of the MBD proteins have the ability to recognize methylated CpGs in vitro and in vivo. In this review, we have described MBD proteins and their functions in a wide range of cellular processes in various plant species. We have also outlined their mechanism of action and interacting protein partners.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 2","pages":"163-171"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11890390/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}