Physiology and Molecular Biology of Plants最新文献

{"title":"Molecular underpinnings of <i>EbMYBP1</i>-mediated plant defense against UV-B radiation.","authors":"Chunfan Xiang, Juan Wang, Pinhan Zhou, Mamtimin Mamat, Eparay Abdisattar, Lesong Li, Yan Zhao","doi":"10.1007/s12298-025-01598-y","DOIUrl":"10.1007/s12298-025-01598-y","url":null,"abstract":"<p><p>MYB transcription factors play an important role in the response of plants to abiotic stress<b>.</b> The flavonoids found in <i>Erigeron breviscapus</i> have significant anti-inflammatory and cardiovascular therapeutic effects. It has been discovered that <i>EbMYBP1</i>, a gene cloned from <i>E.breviscapus</i>, positively regulates flavonoid synthesis. However, it is uncertain whether <i>EbMYBP1</i>-OE directly responds to ultraviolet B (UV-B) by increasing flavonoids accumulation. Here, an integrated metabolome-transcriptome analysis revealed an important role for <i>EbMYBP1</i> in transgenic tobacco seeds in response to UV-B. The role of <i>EbMYBP1</i> under UV-B has been examined. The results showed that a higher level of UV-B tolerance was observed in seedlings and leaves of <i>EbMYBP1</i>-OE lines (OE8, OE10, OE15) than in wild-type line (WT), identifying several flavonoid biosynthesis genes and metabolites. Compared with WT, a significant decrease in reactive oxygen species (ROS), an increase in antioxidant enzyme expression, and significant induction of genes involved in flavonoids synthesis, UV-B response, and ROS was observed after UV-B treatment in <i>EbMYBP1</i>-OE lines. Overall, <i>EbMYBP1</i> modulates ROS scavengers and upregulates stress response genes to increase UV-B tolerance.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01598-y.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 4","pages":"609-622"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144182299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological, biochemical, and biophysical changes in chia seeds during accelerated aging: implications for lipid composition and seed quality.","authors":"María Emilia Rodríguez, Ethel Pérez, Martín Moisés Acreche, Aline Schneider-Teixeira, Lorena Deladino, Vanesa Ixtaina","doi":"10.1007/s12298-025-01595-1","DOIUrl":"10.1007/s12298-025-01595-1","url":null,"abstract":"<p><p>Chia, an oilseed native to Mexico and Guatemala, is prized for its nutrition and versatile uses in food and industry. Ex situ conservation of chia seeds is vital, yet their high lipid content complicates long-term storage. This study investigates artificial aging's impact on chia seed quality, emphasizing oxidative stress effects on lipid composition, antioxidants, and physiological properties. Two chia genotypes -one with mixed seed colors (MN) and another exclusively white (WN)- were subjected to accelerated aging to analyze germination, growth, electrical conductivity, and biochemical and biophysical changes over time. Accelerated aging revealed stress tolerance in chia seeds but significantly impacted germination and biochemical composition. Germination decreased from 100 to 0% over 56 days, with reduced radicle and hypocotyl lengths, fewer normal seedlings, and more abnormal or dead seeds. Peroxide values rose significantly, from 1.81 to 6.50 meq.kg^-1 (WN) and 0.85 to 3.22 meq.kg^-1 (MN), while free fatty acids increased from 0.41 to 2.95% oleic (WN) and 0.40 to 3.18% oleic (MN). Tocopherol content decreased markedly, disrupting the antioxidant-prooxidant balance. These biochemical changes resulted in higher saturated fatty acids, reducing membrane fluidity, and increasing electrical conductivity from 129.26 to 399.25 μS.cm^-1.g^-1 (WN) and 177.06 to 500.81 μS.cm^-1.g^-1 (MN). Thermal properties analyzed by DSC highlighted transitions within -90 to 100 °C, while FTIR spectroscopy revealed viability-related changes, particularly in the 1740 cm^-1 region. These findings underscore the impact of oxidative stress on seed quality, posing challenges for conservation and commercialization and emphasizing the need for strategies to mitigate storage-related deterioration.</p><p><strong>Graphic abstract: </strong></p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 4","pages":"623-640"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116978/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analyses reveal <i>TaWRKY41</i> as a potential candidate governing spot blotch resistance in wheat.","authors":"Bhakti R Dayama, Varsha A Mahadik, Deepika Somani, Balkrishna A Shinde, Kirtikumar R Kondhare, Muthukumarasamy Karthikeyan, Narendra Y Kadoo","doi":"10.1007/s12298-025-01583-5","DOIUrl":"10.1007/s12298-025-01583-5","url":null,"abstract":"<p><p>Spot blotch disease caused by <i>Bipolaris sorokiniana</i> poses a significant threat to wheat production. Cultivation of disease-resistant wheat genotypes appears to be the most practical approach to mitigate the impact of this devastating disease. However, the molecular responses of wheat plants during spot blotch disease progression remain poorly understood. This study employed RNA-sequencing to unravel the spatiotemporal molecular events underlying the resistance mechanism in the spot blotch susceptible and resistant wheat genotypes. This study further provides a comprehensive overview of differentially expressed transcripts through functional analysis and transcription factor identification, elucidating the biological mechanisms governing wheat-<i>B. sorokiniana</i> interaction. In the resistant genotype, the expression of one of the key transcription factors, <i>TaWRKY41</i>, was significantly induced upon pathogen inoculation. Computational studies, electrophoretic-mobility shift assay, and yeast one-hybrid assay confirmed the interaction of the recombinant TaWRKY41 protein with W-box elements present in the promoters of plant defense-related genes. Furthermore, co-expression network analyses identified downstream genes positively correlated with <i>TaWRKY41</i>, providing insights into their probable involvement in the defense response. Overall, our investigation suggests that <i>TaWRKY41</i> contributes to spot blotch resistance in wheat. This knowledge can help develop new disease-resistant wheat varieties.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01583-5.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 4","pages":"591-608"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116962/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insight into polyethylene glycol-mediated physiochemical, nutritional, and antioxidative defense modulations in salt-stressed <i>Raphanus sativus</i> L.","authors":"Muhammad Sajid, Shakil Ahmed, Rehana Sardar, Nasim Ahmad Yasin","doi":"10.1007/s12298-025-01585-3","DOIUrl":"10.1007/s12298-025-01585-3","url":null,"abstract":"<p><p>Salinity is one of the most crucial factors that impede various morphological and physiological parameters, eventually reducing crop production. Chemical and physical weathering, in addition to poor irrigation practices, enhances soil salinity. Radish (<i>Raphanus sativus</i> L<i>.</i>), a leafy and root vegetable, is cultivated worldwide because of its nutritional value. However, salinity poses a serious threat to its productivity. Polyethylene glycol (PEG) is mainly used to induce and study osmotic stress in plants. However, our novel research work was designed to observe the stress-mitigating potential of PEG (10%, 20%, 30%, and 40% PEG) in <i>R. sativus</i> subjected to salinity stress (200 mM NaCl). Salt toxicity significantly reduced the seed germination (61.03%), seedling vigor index (54.25%), total soluble protein (69.23%), and biomass accumulation (42.25%) of <i>R. sativus</i> plants. Similarly, stressed plants presented a reduced synthesis of photosynthetic pigments and poor nutrition. However, seed priming with PEG-30% significantly alleviated salt stress by promoting growth attributes, mineral uptake, and the antioxidative defence system of <i>R. sativus</i> under salinity regimes. Plants raised from seeds treated with 30% PEG alleviated NaCl-induced oxidative stress by modulating the activity of antioxidative enzymes such as peroxidase, ascorbate peroxidase, glutathione peroxidase, glutathione S-transferase, ascorbic acid, superoxide dismutase, and catalase. Furthermore, PEG-30% significantly improved photosynthetic pigment biosynthesis, although there was a decrease in electrolyte leakage and lipid peroxidation in plants under saline conditions. Furthermore, 30% PEG improved the shoot length (41.46%), root length (46.57%), and biomass production (53.93%) of salt-stressed plants. This study revealed that 30% PEG is beneficial for reversing salt stress. However, extensive field studies are required to assess the potential of PEG for mitigating salt stress in various geographical regions.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01585-3.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 4","pages":"659-674"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116968/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144183695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon assimilation dynamics in <i>Pontederia crassipes</i> in response to light intensity and CO₂ levels.","authors":"Bianca Jaqueline Santos Rodrigues, Marcos Antonio Bacarin, Junior Borella","doi":"10.1007/s12298-025-01587-1","DOIUrl":"10.1007/s12298-025-01587-1","url":null,"abstract":"<p><p><i>Pontederia crassipes</i> Mart. is an aquatic macrophyte native to South America, tolerant to high intensity of sunlight and has spread to various countries worldwide. This study aimed to investigate the photosynthetic characteristics of <i>P. crassipes</i> leaves under different light intensities and CO₂ concentrations by analyzing net photosynthetic response, Rubisco activity, and related photosynthetic parameters. The plants were acclimated in a greenhouse under natural light conditions at a temperature of 25 ± 5 °C. The light response curve was measured using a portable infrared gas exchange system, coupled with the 6400-40 Leaf Chamber Fluorometer. The response of net photosynthesis to intercellular CO₂ concentration was determined at 1500 µmol m^-2 s^-1 of photosynthetic active radiation (PAR). <i>P. crassipes</i> demonstrated a remarkable capacity for adaptation to varying light intensities and CO₂ concentrations, exhibiting strong photosynthetic efficiency, as indicated by its net CO₂ assimilation rate in response to PAR, sustained electron transport rate up to 1000 µmol photons m^-2 s^-1, a positive correlation between φPSII and φCO₂, and a high net CO₂ assimilation rate in response to <i>C</i> _<i>i</i> .</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 4","pages":"641-645"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116967/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144182093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic analysis of the physiological and molecular responses of foxtail millet (<i>Setaria italica</i>) to cadmium stress.","authors":"Xingqi Chen, Kexin Xu, Shanshan Heng, Yuqing Zhong, Jiajia Chen, Xin Qi","doi":"10.1007/s12298-025-01582-6","DOIUrl":"10.1007/s12298-025-01582-6","url":null,"abstract":"<p><p>Foxtail millet (S<i>etaria italica</i>) is a globally distributed crop rich in essential nutrients, serving as an important source of food and feed. However, its growth and productivity are increasingly threatened by cadmium (Cd) pollution. In this study, to investigate the physiological and molecular responses of foxtail millet to Cd stress, seedlings were treated with 0, 250 or 500 μM Cd²⁺ for 1, 2 or 3 days. Their morphological, physiological, ultrastructural, and molecular responses were systematically analyzed. The results showed obvious morphological changes, including leaf darkening, reduced vitality, and shoot dwarfing, with more severe effects observed at higher Cd concentrations. Notably, Cd stress led to a significant increase in proline content, relative electrical conductivity, and malondialdehyde levels in both shoots and roots, accompanied by dramatic changes in the activities of antioxidant enzymes including peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT). Ultrastructural analysis further revealed severe cellular damage, characterized by distorted chloroplasts in leaves and shrunken root tips. Transcriptomic profiling identified differentially expressed genes in both shoots and roots, which were significantly enriched in pathways related to oxidative stress response, photosynthesis, and metal ion transport. Additionally, a genome-wide analysis identified eight <i>SiNRAMP</i> genes in foxtail millet, among which <i>SiNRAMP5</i> and <i>SiNRAMP8</i> were significantly upregulated in both shoots and roots under Cd exposure. These findings provide new insights into the physiological, ultrastructural, and molecular responses of foxtail millet to Cd stress and underscore the potential roles of SiNRAMP in Cd detoxification and tolerance mechanisms.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 4","pages":"647-658"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116973/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144182055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous sucrose alleviates salt stress in sunflower (<i>Helianthus annuus</i> L.) and canola (<i>Brassica napus</i> L.) by modulating osmotic adjustment and antioxidant defense system.","authors":"Büşra Sevgi, Sema Leblebici","doi":"10.1007/s12298-025-01571-9","DOIUrl":"https://doi.org/10.1007/s12298-025-01571-9","url":null,"abstract":"<p><p>Salinity, a major ecological problem worldwide, adversely affects plant growth and productivity. Osmoprotectants are a possible strategy for plants to cope with and regulate their response to unfavorable environmental conditions, such as salinity. However, the role of sucrose in this process requires more precise elucidation. This study aims to investigate the ameliorative role of sucrose on growth parameters, proline content, antioxidant enzyme activity, and gene expression in sunflower and canola under salt stress. The treatments included a 3% sucrose concentration and two levels of salinity (75 and 150 mM NaCl). Salinity caused a remarkable reduction in stem-root growth, chlorophyll amounts and catalase (CAT) activity, whereas it unchanged ascorbate peroxidase (APX) activity. Furthermore, both plants grown under salt stress had considerably higher total protein, proline, malondialdehyde (MDA) content, and superoxide dismutase (SOD) activity. Exogenous sucrose increased plant growth, chlorophyll amounts and the activities of hydrogen peroxide-detoxifying antioxidant enzymes such as CAT and APX in salt-stressed plants, but dramatically depressed levels of osmoregulators such as protein and proline. Besides that, it balanced antioxidant enzyme levels by regulating SOD activity to the required level, thereby facilitating the effective operation of the antioxidant defense system. Additionally, sucrose had a different effect on gene expressions of antioxidants in sunflower and canola under salinity. These results revealed that sucrose can ameliorate the deleterious effects of salinity in sunflower and canola by modulating osmotic substance accumulation, the activity of antioxidant enzymes, and their gene expression. In conclusion, sucrose can be a potential tool for plants in salt stress alleviation.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01571-9.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 3","pages":"405-418"},"PeriodicalIF":3.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006602/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification, expression analyses of <i>APETALA1</i> gene homologs in <i>Bambusa tulda</i> and heterologous validation of <i>BtMADS14</i> in <i>Arabidopsis thaliana</i>.","authors":"Mridushree Basak, Sukanya Chakraborty, Sutrisha Kundu, Sonali Dey, Malay Das","doi":"10.1007/s12298-025-01569-3","DOIUrl":"10.1007/s12298-025-01569-3","url":null,"abstract":"<p><p>Bamboos belong to the grass family Poaceae, sub-family Bambusoideae and possess many interesting developmental features including a long vegetative period before flowering. Previously, transcriptome based analyses have identified differentially expressed transcripts in flowering and vegetative tissues to predict gene clusters of functional relevance. In contrast, limited studies were conducted to characterize individual genes to decipher their precise role to induce flowering. This was primarily due to the unavailability of sufficient genomic resources, which has lately been overcome by the release of additional bamboo genomes. In this study, the <i>APETALA1</i> gene homologs (<i>MADS14</i>, <i>MADS15</i>, <i>MADS18</i> and <i>MADS20</i>) have been identified from five sequenced bamboo species (<i>Bonia amplexicaulis</i>, <i>Guadua angustifolia</i>, <i>Raddia guianensis</i>, <i>Olyra latifolia</i>, <i>Phyllostachys edulis</i>). In addition, <i>APETALA1</i> homologs from a tropical bamboo (<i>Bambusa tulda</i>) have been PCR amplified, sequenced and included in the analyses to widen spectrum of sampling. Assessment of their phylogenetic and syntenic relationship with related Poaceae neighbours revealed closer relationship between <i>MADS14</i> and <i>MADS15</i> members than <i>MADS18</i> and <i>MADS20</i>. Transcriptional expression patterns of <i>B. tulda BtMADS14</i>, <i>BtMADS15</i>, <i>BtMADS18</i> and <i>BtMADS20</i> in vegetative and floral tissues indicated a possible role of <i>BtMADS14</i> and <i>BtMADS15</i> in flower induction and differentiation, while <i>BtMADS18</i> might be associated with seed development. Total 24 proteins were predicted to interact with the <i>Phyllostachys edulis</i> homolog of BtMADS14 protein and 8 of them were members of the MADS-box family. The <i>p35S::BtMADS14</i> overexpressing Arabidopsis plants flowered 8-10 days earlier than the wild type plants suggesting its possible involvement in the floral induction of <i>B. tulda</i>.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01569-3.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 3","pages":"389-404"},"PeriodicalIF":3.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006657/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"De novo SSR markers development and genetic diversity assessment in <i>Ailanthus excelsa</i> in India.","authors":"Rama Kant, Rajendra K Meena, Rimpee Garg, Vikas, Abhishek Yadav, Maneesh S Bhandari","doi":"10.1007/s12298-025-01566-6","DOIUrl":"10.1007/s12298-025-01566-6","url":null,"abstract":"<p><p><i>Ailanthus excelsa</i> is a fast-growing, multipurpose agroforestry tree species of Indian Arid Regions (IAR). It is widely cultivated as tree outside forests (TOFs) on farm lands, roadside, canal banks, etc., where the genetic stocks were randomly planted. To ensure the availability of quality planting materials (QPM) for industrial profitability, the germplasm must undergo a systematic genetic improvement program. Genetic variability in the base population is crucial for effective selection, but the lack of genomic resources and marker impedes this process. This study aimed to generate genome sequence information and de novo development of simple sequence repeats (SSRs) in <i>A. excelsa</i>. About 96 million raw reads were generated using Illumina platform, assembled into ~ 183,000 contigs with 33% GC content and an N50 value of 641 bp. A total of 7,667 microsatellite repeats were identified, with di-nucleotides being the most abundant. AT rich repeats were more prevalent than GC rich motifs. A total of 3,696 primer pairs were designed, and 150 of these were selected for validation. In PCR, 145 SSRs were positively amplified and 15 showed polymorphic banding pattern. These polymorphic SSRs were used to characterize 213 individuals from northern and central India. SSR analysis revealed high gene diversity (H_e = 0.71; A_r = 9.12) with negligible genetic differentiation in populations. The study presents a comprehensive set of de novo SSR markers and provides baseline knowledge of genetic structure of <i>A. excelsa</i>, essential for conservation and long-term genetic improvement programs.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01566-6.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 3","pages":"357-373"},"PeriodicalIF":3.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The transcriptional regulation of Arabidopsis <i>ECT8</i> by ABA-Responsive Element binding transcription factors in response to ABA and abiotic stresses.","authors":"Jiexuan Zheng, Diandian Wen, Chuang Tang, Sihui Lai, Yujie Yan, Chang Du, Zhonghui Zhang","doi":"10.1007/s12298-025-01565-7","DOIUrl":"10.1007/s12298-025-01565-7","url":null,"abstract":"<p><p><i>N</i> ⁶-methyladenosine modification is a critical epigenetic mark in the plant response to abscisic acid (ABA) and various abiotic stresses including salinity, drought, and cold stresses. Arabidopsis Evolutionarily Conserved C-Terminal Region 8 (ECT8), an m⁶A reader, has been reported to participate in the response to ABA and salinity stress. However, the intricate regulatory mechanisms governing <i>ECT8</i> expression in these stress responses have not been fully elucidated. Our multidisciplinary analyses have revealed that <i>ECT8</i> exhibits a broad expression pattern across tissues, with particularly high levels observed in senescent leaves. Furthermore, <i>ECT8</i> expression is markedly upregulated in response to ABA, salinity, and osmotic stress. Intriguingly, the promoter region of the <i>ECT8</i> gene harbors two ABA Responsive Elements (ABREs). Employing yeast one-hybrid assays, we identified that key ABRE-binding transcription factors within the ABA signaling cascade, namely ABA INSENSITIVE 5 (ABI5) and ABRE BINDING FACTOR1/2/3/4 (ABF1/2/3/4), exhibit a specific binding affinity for the <i>ECT8</i> promoter, with the two ABREs indispensable for their interaction. The Dual-Luciferase Reporter assay and Chromatin immunoprecipitation assay confirmed their interaction <i>in planta</i>. The expression pattern of <i>ECT8</i> in mutants deficient in the core components of the ABA signaling pathway indicated that <i>ECT8</i> is modulated by ABI5/ABF-mediated ABA signaling. Collectively, our findings elucidate the feedback mechanism linking ABA perception to the regulation of <i>ECT8</i> expression, thereby shedding new light on the intricate interplay between ABA signaling and RNA m⁶A modification. This discovery enriches our understanding of the molecular crosstalk that underpins plant stress responses.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01565-7.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 3","pages":"343-355"},"PeriodicalIF":3.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144032000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}