Physiology and Molecular Biology of Plants最新文献

{"title":"Daylength extension: a strategy to enhance sucrose metabolism and carbohydrate accumulation for improving spike quality and corm production in Gladiolus (<i>Gladiolus hybridus</i> Hort.).","authors":"Manisha Chumber, Shalini Jhanji","doi":"10.1007/s12298-025-01602-5","DOIUrl":"10.1007/s12298-025-01602-5","url":null,"abstract":"<p><p>Daylength extension (DLE) has significant potential to enhance the growth and development of various crops through increased carbohydrate synthesis and metabolism. This study explored the impact of DLE on spike quality, corm production, sucrose metabolism and carbohydrate accumulation of Gladiolus. Plants were subjected to DLE of 2 and 4 h after 30, 40 and 50 days of sprouting. The 2 h of DLE after 50 days of sprouting markedly increased acid invertase, neutral invertase and sucrose synthase activities in leaves by 1.11, 1.88 and 3.53 times and in corms by 1.44, 1.83 and 1.84 times, respectively, compared to natural daylength (NDL). Carbohydrate accumulation improved, with most pronounced effects of DLE after 30 and 50 days of sprouting. The DLE of 2 h also led to early spike development. The DLE of 2 h after 50 days, enhanced spike length (11.54%), weight (14.25%), diameter (11.86%) and floret size (10.98%) compared to NDL. Corm production was significantly higher under 2 h of DLE after 50 days, as corm (97.59 g) and cormel weight per plant (6.33 g), corm diameter (52.82 mm) and number of corms (2.17) and cormels per plant (29.00) increased compared to NDL. Correlation and Principal Component Analysis revealed that improved spike quality and corm production was result of enhanced sucrose enzyme activity, increased carbohydrate accumulation and earlier spike development. Therefore, 2 h of DLE after 30 or 50 days of sprouting could be effective in enhancing corm production and spike quality. Thus, considering economic benefits, employing 2 h of DLE after 50 days of sprouting could be recommended.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01602-5.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 5","pages":"795-812"},"PeriodicalIF":3.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12185823/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144497674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of genetic variability and emerging strains of <i>Okra enation leaf curl virus</i>: increasing risks to okra production in India.","authors":"Ankit Kumar, Jyoti Singh, Anupma Singh, Dharmendra Pratap","doi":"10.1007/s12298-025-01578-2","DOIUrl":"10.1007/s12298-025-01578-2","url":null,"abstract":"<p><p>Okra enation leaf curl disease (OELCuD), caused by <i>Okra enation leaf curl virus</i> (OELCuV) and transmitted by whiteflies (<i>Bemisia tabaci</i>), significantly threatens okra cultivation in India. This study conducted a comprehensive survey (2020-2022) across seven Indian states, recording disease incidence ranging from 14.03% to 67.57%. Polymerase chain reaction (PCR) using coat protein gene-specific primers confirmed the presence of OELCuV in symptomatic plants, amplifying a ~ 750 bp fragment. Full-genome characterization of five isolates from different geographic regions using rolling circle amplification (RCA) revealed high genetic variability, with nucleotide identities ranging from 92.2% to 96.5% compared to existing OELCuV DNA-A sequences. Two novel strains were identified in Meerut (Uttar Pradesh) and Viluppuram (Tamil Nadu), meeting the International Committee on Taxonomy of Viruses (ICTV) species demarcation criteria. Phylogenetic and recombination analyses demonstrated that these strains form a unique cluster with recombinant features, particularly in the AC1 coding region, which is under purifying selection. The findings underscore the urgent need to monitor the genetic variability and spread of OELCuV to protect okra cultivation from evolving viral threats.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01578-2.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 5","pages":"767-783"},"PeriodicalIF":3.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12185807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144497626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of CRISPR construct activity and gene function in melon via a hairy root transformation system.","authors":"Xiang Li, Chenchen Cao, Pablo Bolaños-Villegas, Ying Liu, Jiyu Wang, Qiong Li, Wenwen Mao, Panqiao Wang, Juan Hou, Lili Li, Jianbin Hu, Yonghua Li","doi":"10.1007/s12298-025-01607-0","DOIUrl":"10.1007/s12298-025-01607-0","url":null,"abstract":"<p><p>Melon (<i>Cucumis melo</i> L.), an important cash fruit crop with high nutritional value, is cultivated worldwide. To promote the application of gene editing technology and accelerate functional analysis of genes in melon, we developed an efficient protocol for inducing the formation of hairy roots. Using melon cotyledon as explants and <i>Agrobacterium rhizogenes</i> (<i>A. rhizogenes</i>) K599 as the engineering bacterium, a large number of hairy roots could be induced within a month and the transformed hairy roots accounted for 68.61% of the total hairy roots. On average, 2.61 positive hairy roots were formed on each explant. By transforming hairy roots with a CRISPR/Cas9 gene editing construct, the availability of target sites can be assessed <i>in planta</i> in a brief time. The gene editing targets are preliminarily divided into three types: full editing, partial editing, and no editing, and the efficacy of target sites was further validated by stable transformation. Then, we found that the efficiency of gene editing was promoted by the number of sgRNA expression cassettes. Finally, we used this system to analyze the function of melon <i>CmRHL1</i> in root hair development and found that melon root hair development was significantly inhibited by the mutation of this gene. In summary, the hairy root editing method established in this study may be used to quickly validate the activity of CRISPR/Cas9 constructs and characterize gene function during root development, serving as a complementary tool for heritable genome editing in melon.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01607-0.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 5","pages":"753-766"},"PeriodicalIF":3.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12185833/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144497627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation and identification of reference genes for qRT-PCR analysis in bermudagrass roots under alkaline salt stress.","authors":"Lisi Tang, Qikun Yu, Wen Li, Zongjiu Sun, Chao Fu, Guozhi Hu, Zhengfa Yu, Shirui Ma, Peiying Li","doi":"10.1007/s12298-025-01603-4","DOIUrl":"10.1007/s12298-025-01603-4","url":null,"abstract":"<p><p>Quantitative real-time PCR (qRT-PCR) is a potent technique for gene expression analysis, but its accuracy relies heavily on the selection of stable reference genes. In bermudagrass (<i>Cynodon dactylon</i>) roots under alkaline salt stress, we sought to identify suitable reference genes. Seven candidates-<i>EF1α</i>, <i>PP2A</i>, <i>TIP41</i>, <i>GAPDH</i>, <i>Actin</i>, <i>β-tubulin</i>, and <i>CACS</i>-were assessed for specificity, amplification efficiency, and expression stability using geNorm, NormFinder, BestKeeper, and RefFinder. All primers exhibited high specificity and efficiency, as evidenced by single, strong bands on agarose gels and single melting peaks in qRT-PCR. Initial ΔCt value analysis identified <i>EF1α</i>, <i>TIP41</i>, and <i>GAPDH</i> as the most stable genes, with further analysis consistently ranking <i>EF1α</i> as the top reference gene across all software. Validation through transcriptome data and qRT-PCR of selected core genes confirmed <i>EF1α</i>'s stability and suitability for gene expression studies in bermudagrass roots under stress. This study offers a thorough evaluation of reference genes for qRT-PCR in bermudagrass and enhances our comprehension of gene expression quantification in this species under alkaline salt stress.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01603-4.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 5","pages":"729-738"},"PeriodicalIF":3.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12185821/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144497678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Empowering rice resilience: elevating starch quality via seed priming amidst bio-molecular challenges during reproductive and maturation phases under drought stress.","authors":"Mahesh Kumar Samota, Monika Awana, Rakesh Pandey, S V Amitha Mithra, Veda Krishnan, Suresh Kumar, Aruna Tyagi, Archana Singh","doi":"10.1007/s12298-025-01609-y","DOIUrl":"10.1007/s12298-025-01609-y","url":null,"abstract":"<p><p>Drought is a major global limiting factor for rice (<i>Oryza sativa</i> L.) production. Drought conditions reduce the quality and yield of rice. In the current research, we explored the effects of diverse seed priming agents and their combinations on multiple facets of grain quality, bio-molecular mechanisms, and enzyme activities. Combinations of the priming agents like MJ (methyl jasmonate) + Zinc sulphate heptahydrate (MJZ), MJ + Iron sulphate heptahydrate (MJI), and MJ + Zinc sulphate heptahydrate + Iron sulphate heptahydrate (MJIZ) were used for the seed priming. High performance liquid chromatography based abscisic acid content analysis showed 3.16 and 2.56-fold higher amounts in MJ-primed samples in N-22 and PS-5, respectively compared to unprimed controls. In unprimed controls, N-22 had lower amylose content (4.4%) than PS-5 (4.8%). The resistant starch content was increased in MJIZ primed samples by 253% in control samples in comparison to their unprimed samples. Enzyme activity assays revealed that pullulanase, activity increased significantly with priming and was found to be highest in MJIZ primed samples. Starch Synthase, and branching enzyme activity increased significantly in both genotypes with priming compared to unprimed controls. Gene expression analyses revealed upregulation of <i>SS-I</i>, <i>SS-IIa</i>, and <i>Pull-2</i> genes in primed samples, indicating enhanced starch biosynthesis. Scanning electron microscopy-based starch granule morphology revealed differences in shape, size, and packing across the treatments and genotypes. The findings unravel the mechanisms triggered by seed priming to enhance the adaptability of plants for better survival under drought. To the best of our knowledge, this is the first comprehensive study to decipher the underlying mechanisms through physio-biochemical and molecular analysis on seed priming to alleviate the deleterious effect of drought stress. Being a cost-effective and user-friendly technique, seed priming can be utilized to improve the yield potential of rice under drought, which might help to achieve Sustainable Development Goal 2 (Zero hunger with improved nutrition) of the United Nations.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01609-y.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 5","pages":"835-849"},"PeriodicalIF":3.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12185800/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144497676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pigments to precision: RUBY aiding genetic transformation and genome editing in wheat and barley.","authors":"Manas Ranjan Prusty, Arava Shatil-Cohen, Rakesh Kumar, Davinder Sharma, Anna Minz-Dub, Smadar Ezrati, Avigail Hihinashvili, Amir Sharon","doi":"10.1007/s12298-025-01591-5","DOIUrl":"10.1007/s12298-025-01591-5","url":null,"abstract":"<p><p>Genetic engineering of wheat is complex due to its large genome size, the presence of numerous genes with high sequence similarities, and a multitude of repetitive elements. In addition, genetic transformation of wheat has been difficult, mainly due to poor regeneration in tissue cultures. Recent advances in plant biotechnology, particularly the use of the regenerative genes GROWTH-REGULATING FACTOR (<i>GRF</i>) and GRF-INTERACTING FACTOR (<i>GIF</i>), have provided new tools for wheat transformation and regeneration. Another transformative tool is the RUBY system that involves genetic engineering of three betalain biosynthesis genes, providing a noninvasive, visually detectable red pigment. In this study, we used the <i>GRF4-GIF1</i> chimera along with the RUBY system to advance transformation and gene editing in wheat and barley. The <i>GRF4-GIF1</i> chimera significantly aided wheat regeneration; however, it had an opposite effect in barley, where it inhibited the regeneration process. Therefore, we generated RUBY transgenic barley lines using constructs that did not include the <i>GRF4-GIF1</i> chimera. Additionally, we used the RUBY cassette for fast assessment of gene editing by knockingout the first betalain biosynthetic gene in RUBY- positive transgenic wheat plants, resulting in a change of leaf color from red to green. The edited RUBY wheat lines lost more than just the red color. They also lost betalain-related traits, such as being less likely to get leaf rust (<i>Puccinia triticina</i>) and salt stress. Importantly, the loss of RUBY did not affect plant viability, making it a useful tool for genome editing and a viable alternative to destructive methods.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01591-5.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 4","pages":"545-554"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116407/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cd²⁺ and Zn²⁺ regulating uptake and accumulation of TDCPP and TMPP in rice (<i>Oryza sativa</i> L.) in transcript and protein level.","authors":"Mengyao Wu, Haiou Wang, Wenxuan Wang, Xiaoyu Ren, Juming Zhang","doi":"10.1007/s12298-025-01589-z","DOIUrl":"10.1007/s12298-025-01589-z","url":null,"abstract":"<p><p>Hydroponic experiments and computational simulations were conducted to investigate the changes in the gene expression, structure, and binding mode of the rice transporter protein OsTIL to OPEs by the presence of Cd²⁺ and Zn²⁺. OPEs and Zn²⁺ were observed to promote seedling growth and OPEs alleviated the suppressive effect of Cd²⁺ on seedlings. Usually, Cd²⁺ and Zn²⁺ inhibited the accumulations of OPEs in plants accompanied by the decrease of RCF (root concentration factor) and TF (transport coefficient). In particular, Zn²⁺ promoted TDCPP accumulation only in roots accompanied by the increase of RCF and the decrease of TF. Furthermore, Cd²⁺ and Zn²⁺ affected the gene expressions of OPEs transporter-OsTIL response in the accumulation of OPEs in both single and compound pollution. After molecular dynamics simulation analysis, RMSD of the protein backbone, binding pocket, and ligand only in TDCPP-OsTIL complex also were significantly affected by two metal ions. Furthermore, two metal ions can press the interaction of OPEs and OsTIL by reducing the stability of protein structure and the numbers of HB (hydrogen bonds) and enlarging the pocket. However, Zn²⁺ extra supports an enlarged entrance for TDCPP, which could facilitate the entry of the coordination complex of Zn²⁺ with TDCPP into the pocket and improve TDCPP capacity of OsTIL.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01589-z.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 4","pages":"555-570"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature stress and its effects on phytochemical dynamics and antioxidant activity in <i>Withania somnifera</i> (L). dunal.","authors":"Ankita Singh, Usha Mina","doi":"10.1007/s12298-025-01594-2","DOIUrl":"10.1007/s12298-025-01594-2","url":null,"abstract":"<p><p>Elevated temperature stress limits the growth, metabolism and productivity of medicinal plants. However, the response of <i>Withania somnifera</i> (L.) Dunal (Ashwagandha) which has diverse therapeutic properties, to elevated temperature stress remains unexplored. This study investigated the effects of elevated temperature stress exposure on the phytochemical content and antioxidant activity of Ashwagandha four varieties namely: Vallabh 01, Vallabh 02, Pratap, and Chetak, across leaf, stem, root, and fruit parts. The selected varieties were exposed to ambient temperature (10.4 ± 2.6 to 31.6 ± 5.9 ℃) and elevated temperature (ET) levels (13.5 ± 3.5 to 34.3 ± 5.6 ℃) in net house and control environment facility chamber, respectively from November 2021 to April 2022. Fruiting stage samples of different parts of all varieties from both treatments were analysed for total phytochemical content, total flavonoid content (TFC) and antioxidant activity. The results revealed the variable response of varieties and their parts to ET stress. ET stress enhanced the cumulative (root + stem + leaf + fruit) phytochemical content of Vallabh 02, Pratap and Chetak by 112.9%, 15.2%, and 84.9%, respectively, and suppressed in the Vallabh 01 (3.6%). TFC and antioxidant activity were significantly (p < 0.05) higher under ET stress in all the varieties in the following order Pratap > Chetak > Vallabh 01 > Vallabh 02. The findings indicate that ET stress exposure significantly alters as well as enhances Ashwagandha varieties' total phytochemical content and antioxidant activity. Among the four varieties, Pratap is the most tolerant to ET stress. This study indicates that climate change associated ET levels up to 2 ℃ may enhance therapeutic potential of Ashwagandha varieties through synthesis of novel and unique phytochemicals. Novel knowledge of ET levels exposure to favour the production of specific phytochemicals can aid in optimizing the cultivation of medicinal plants for higher yields of desired medicinal compounds.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01594-2.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 4","pages":"675-692"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring pharmacological potential of <i>Aegle marmelos</i>: integrating traditional knowledge with modern physiology and molecular biology.","authors":"Manish Thakur, Tejinder Kaur, Ranbir Chander Sobti","doi":"10.1007/s12298-025-01586-2","DOIUrl":"10.1007/s12298-025-01586-2","url":null,"abstract":"<p><p><i>Aegle marmelos</i> is a plant that holds considerable medicinal value. It is indigenous to India, Iran, Myanmar, Pakistan, Bangladesh, and various Southeast Asian countries. The sacred tree, which has its historical roots in the Vedic era dating back to 2000 B.C., holds a significant place in various traditional systems of medicine due to its extensive therapeutic properties that have been recognized over time. The plant exhibits a diverse array of bioactive compounds, namely flavonoids, alkaloids, polyphenols, terpenoids, carotenoids, and coumarins, which are present in various parts of the plant, including leaves, flowers, bark, and fruit. These compounds are responsible for the plant's extensive range of medicinal properties. It has been observed that <i>A. marmelos</i> demonstrates various biological activities including anti-proliferative, anti-pyretic, anti-inflammatory, anti-fungal, anti-diarrhoeal, and antimicrobial properties. Recent scientific investigations have provided confirmation regarding the effectiveness of the substance under investigation against a wide range of disease-causing microorganisms. These microorganisms include bacteria such as <i>Micrococcus luteus</i> and <i>Streptococcus faecalis</i>, as well as fungi like <i>Aspergillus fumigatus</i> and <i>Candida albicans</i>. The phytochemical profile of <i>A. marmelos</i> includes the presence of marmenol, marmin, marmelosin, and several other compounds that are known to contribute to its medicinal properties. The present review aims to comprehensively synthesize the existing body of research on <i>Aegle marmelos,</i> focusing on its botanical characteristics, phytochemical composition, and wide-ranging medicinal applications.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01586-2.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 4","pages":"521-543"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116412/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated transcriptome and metabolome analyses reveal regulation mechanism of biomass and non‑structural carbohydrate allocation in <i>Emmenopterys henryi</i> Oliv. under shade.","authors":"Wenna Liu, Ruili Luo, Hongwei Wang, Yu Jing, Huaqiang Zhao, Weina Zou, Meifang Hou, Lili Song","doi":"10.1007/s12298-025-01588-0","DOIUrl":"10.1007/s12298-025-01588-0","url":null,"abstract":"<p><p>The adaptability of <i>Emmenopterys henryi</i> Oliv. to shade in the forest is a crucial intrinsic driving force for its natural renewal. Elucidating the influence of shade on biomass and non-structural carbohydrate (NSC) accumulation and allocation in leaf, stem and root will help to understand the endangerment mechanism of <i>E. henryi.</i> Results showed that <i>E. henryi</i> invested more biomass in leaf than in stem and root under shade. The biomass was positively correlated with the NSC pool in leaf, stem and root, respectively. The biomass fraction of leaf, stem or root was positively correlated with NSC fraction in leaf, stem or root of <i>E. henryi</i>, respectively. Starch and sucrose metabolism was proved to be the commonly enriched pathway in leaf, stem and root of <i>E. henryi</i> under shade, the key genes that were regulated differentially by shade. The hub genes regulating accumulation and distribution of biomass and NSC in leaf, stem and root of <i>E. henryi</i> under shade mainly correlated with photosynthesis, respiration, monosaccharides transportation, and cell expansion. Further research into these hub genes will be helpful for illumination of the adaptation mechanism of <i>E. henryi</i> to shade.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01588-0.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 4","pages":"571-590"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116963/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144183696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}