Transcription factor ZdWRKY1 positively promotes the biosynthesis of isoquinoline alkaloids in Zanthoxylum dissitum Hemsl.

IF 3.6 3区生物学 Q1 PLANT SCIENCES

Physiology and Molecular Biology of Plants Pub Date : 2026-08-01 Epub Date: 2026-06-20 DOI:10.1007/s12298-026-01780-w

Congyu Zhang, Yuan Wang, Tingting An, Mingxiu Chen, Wenyan Liu, Fangying Zhou, Xinling Chen, Jikang Sun, Jia Liu

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引用次数: 0

Abstract

Zanthoxylum dissitum Hemsl. (Zanthoxylum) is a traditional Chinese medicine plant usually used to treat gynecological diseases, and isoquinoline alkaloids (IQA) are its most valuable components. In this study, the WRKY transcription factor ZdWRKY1 was identified from Zanthoxylum, and its role in regulating IQA biosynthesis was investigated. Transcriptomic analysis revealed that the expression of ZdWRKY1 was positively correlated with the transcript levels of multiple key IQA biosynthetic genes, including ZdNCS, Zd4'OMT, Zd6OMT, ZdBBE, ZdDBOX, and ZdRNMT. Overexpression of ZdWRKY1 in transgenic hairy roots increased total alkaloid yield by 1.45-fold (15.07 mg/g) and magnoflorine by 1.37-fold (289.68 µg/g) compared to controls. Dual-luciferase reporter and yeast one-hybrid assays further confirmed that ZdWRKY1 activates the transcription of Zd4'OMT by binding to the W-box element in its promoter, thereby regulating the accumulation of IQA.

Supplementary information: The online version contains supplementary material available at 10.1007/s12298-026-01780-w.

查看原文本刊更多论文

转录因子ZdWRKY1正促进花椒中异喹啉生物碱的生物合成。

花椒。花椒（Zanthoxylum）是一种常用的治疗妇科疾病的中药植物，异喹啉生物碱（IQA）是其最有价值的成分。本研究从花椒中鉴定出WRKY转录因子ZdWRKY1，并对其在调控IQA生物合成中的作用进行了研究。转录组学分析显示，ZdWRKY1的表达与多个IQA关键生物合成基因zdnc、zd4’omt、Zd6OMT、ZdBBE、ZdDBOX和ZdRNMT的转录水平呈正相关。ZdWRKY1在转基因毛状根中的过表达使总生物碱产量比对照提高1.45倍（15.07 mg/g）， magnnoflorine产量提高1.37倍（289.68µg/g）。双荧光素酶报告基因和酵母单杂交实验进一步证实，ZdWRKY1通过结合启动子中的W-box元件激活Zd4'OMT的转录，从而调控IQA的积累。补充信息：在线版本包含补充资料，提供地址：10.1007/s12298-026-01780-w。

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来源期刊

Physiology and Molecular Biology of Plants PLANT SCIENCES-

CiteScore

7.10

自引率

0.00%

发文量

126

期刊介绍： Founded in 1995, Physiology and Molecular Biology of Plants (PMBP) is a peer reviewed monthly journal co-published by Springer Nature. It contains research and review articles, short communications, commentaries, book reviews etc., in all areas of functional plant biology including, but not limited to plant physiology, biochemistry, molecular genetics, molecular pathology, biophysics, cell and molecular biology, genetics, genomics and bioinformatics. Its integrated and interdisciplinary approach reflects the global growth trajectories in functional plant biology, attracting authors/editors/reviewers from over 98 countries.