Evaluation of chitosan-saponin nanoparticles for the management of root-knot nematode, Meloidogyne incognita on tomato.

IF 3.6 3区生物学 Q1 PLANT SCIENCES

Physiology and Molecular Biology of Plants Pub Date : 2026-08-01 Epub Date: 2026-06-29 DOI:10.1007/s12298-026-01779-3

Lochan Sharma, Prakash Banakar, Anil Kumar, Ajay Pal, Sarita Sharma

{"title":"Evaluation of chitosan-saponin nanoparticles for the management of root-knot nematode, Meloidogyne incognita on tomato.","authors":"Lochan Sharma, Prakash Banakar, Anil Kumar, Ajay Pal, Sarita Sharma","doi":"10.1007/s12298-026-01779-3","DOIUrl":null,"url":null,"abstract":"The southern root-knot nematode (RKN), Meloidogyne incognita is the major nematode problem in vegetable crops and causes huge amounts of losses in tropical and subtropical regions of the world. The study was carried out to synthesize, characterize, and test the bio-efficacy of chitosan-saponin nanoparticles (CS-SP NPs) against M. incognita on mortality, egg hatching, host finding, and management under growth chamber and screen house conditions on tomato. The NPs were synthesized by the ionic gelation method and characterized by DLS, FTIR, FE-SEM, TEM, XRD and, BET. The NPs exhibited a size of 331 nm, 0.14 PDI, and 36.6 (+ ve) zeta potential. FTIR, FE-SEM, TEM, BET, and XRD confirmed functional groups, spherical shape, large surface area as compared to bulk material, and crystalline structure, respectively. The NPs showed 64.3% mortality with a 930 ppm LC50 value and 45.7% egg hatching inhibition after 24 h. The synthesized NPs also reduced host finding and root parasitism of nematodes in tomato seedlings in the pluronic gel assay. The efficacy of NPs was further validated in a management experiment. Foliar spray of double dose (1860 ppm) of NPs in both the growth chamber and screen house increased plant growth parameters (71.8% and 61.7% fresh shoot weight, respectively) and reduced nematode parameters (39.0% and 39.0% galls, respectively).Supplementary information: The online version contains supplementary material available at 10.1007/s12298-026-01779-3.","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"32 8","pages":"1817-1833"},"PeriodicalIF":3.6000,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13437872/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physiology and Molecular Biology of Plants","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s12298-026-01779-3","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/6/29 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

The southern root-knot nematode (RKN), Meloidogyne incognita is the major nematode problem in vegetable crops and causes huge amounts of losses in tropical and subtropical regions of the world. The study was carried out to synthesize, characterize, and test the bio-efficacy of chitosan-saponin nanoparticles (CS-SP NPs) against M. incognita on mortality, egg hatching, host finding, and management under growth chamber and screen house conditions on tomato. The NPs were synthesized by the ionic gelation method and characterized by DLS, FTIR, FE-SEM, TEM, XRD and, BET. The NPs exhibited a size of 331 nm, 0.14 PDI, and 36.6 (+ ve) zeta potential. FTIR, FE-SEM, TEM, BET, and XRD confirmed functional groups, spherical shape, large surface area as compared to bulk material, and crystalline structure, respectively. The NPs showed 64.3% mortality with a 930 ppm LC₅₀ value and 45.7% egg hatching inhibition after 24 h. The synthesized NPs also reduced host finding and root parasitism of nematodes in tomato seedlings in the pluronic gel assay. The efficacy of NPs was further validated in a management experiment. Foliar spray of double dose (1860 ppm) of NPs in both the growth chamber and screen house increased plant growth parameters (71.8% and 61.7% fresh shoot weight, respectively) and reduced nematode parameters (39.0% and 39.0% galls, respectively).

Supplementary information: The online version contains supplementary material available at 10.1007/s12298-026-01779-3.

查看原文本刊更多论文

壳聚糖-皂苷纳米颗粒防治番茄根结线虫的效果评价。

南根结线虫（Meloidogyne incognita）是危害蔬菜作物的主要线虫，在世界热带和亚热带地区造成了巨大的损失。研究了壳聚糖-皂苷纳米颗粒（CS-SP NPs）的合成、表征，并测试了CS-SP NPs在生长室和筛选室条件下对番茄黑斑夜蛾（M. incognita）的死亡率、卵孵化、寄主发现和管理的生物功效。采用离子凝胶法制备了NPs，并用DLS、FTIR、FE-SEM、TEM、XRD和BET等手段对其进行了表征。NPs的尺寸为331 nm， PDI为0.14，zeta电位为36.6 （+ ve）。FTIR, FE-SEM， TEM， BET和XRD分别证实了其官能团，球形，比表面积大，晶体结构。合成的NPs在24 h后的死亡率为64.3%，LC50值为930 ppm，卵孵化抑制率为45.7%。pluronic gel实验表明，合成的NPs还能降低番茄幼苗的寄主发现率和线虫的根寄生率。在管理实验中进一步验证了NPs的有效性。在生长室和筛房叶面喷施双倍剂量（1860 ppm）的NPs使植株生长参数提高（鲜梢重分别为71.8%和61.7%），线虫参数降低（虫瘿分别为39.0%和39.0%）。补充资料：在线版本包含补充资料，下载地址：10.1007/s12298-026-01779-3。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Physiology and Molecular Biology of Plants PLANT SCIENCES-

CiteScore

7.10

自引率

0.00%

发文量

126

期刊介绍： Founded in 1995, Physiology and Molecular Biology of Plants (PMBP) is a peer reviewed monthly journal co-published by Springer Nature. It contains research and review articles, short communications, commentaries, book reviews etc., in all areas of functional plant biology including, but not limited to plant physiology, biochemistry, molecular genetics, molecular pathology, biophysics, cell and molecular biology, genetics, genomics and bioinformatics. Its integrated and interdisciplinary approach reflects the global growth trajectories in functional plant biology, attracting authors/editors/reviewers from over 98 countries.