Molecular underpinnings of EbMYBP1-mediated plant defense against UV-B radiation.

MYB transcription factors play an important role in the response of plants to abiotic stress. The flavonoids found in Erigeron breviscapus have significant anti-inflammatory and cardiovascular therapeutic effects. It has been discovered that EbMYBP1, a gene cloned from E.breviscapus, positively regulates flavonoid synthesis. However, it is uncertain whether EbMYBP1-OE directly responds to ultraviolet B (UV-B) by increasing flavonoids accumulation. Here, an integrated metabolome-transcriptome analysis revealed an important role for EbMYBP1 in transgenic tobacco seeds in response to UV-B. The role of EbMYBP1 under UV-B has been examined. The results showed that a higher level of UV-B tolerance was observed in seedlings and leaves of EbMYBP1-OE lines (OE8, OE10, OE15) than in wild-type line (WT), identifying several flavonoid biosynthesis genes and metabolites. Compared with WT, a significant decrease in reactive oxygen species (ROS), an increase in antioxidant enzyme expression, and significant induction of genes involved in flavonoids synthesis, UV-B response, and ROS was observed after UV-B treatment in EbMYBP1-OE lines. Overall, EbMYBP1 modulates ROS scavengers and upregulates stress response genes to increase UV-B tolerance.

Supplementary information: The online version contains supplementary material available at 10.1007/s12298-025-01598-y.