Genome-wide association analysis in identification of superior haplotypes for vegetative stage drought stress tolerance in rice.

IF 3.3 3区生物学 Q1 PLANT SCIENCES

Physiology and Molecular Biology of Plants Pub Date : 2025-03-01 Epub Date: 2025-03-23 DOI:10.1007/s12298-025-01573-7

Gyanisha Nayak, C Parameswaran, Nairita Vaidya, Madhuchhanda Parida, S Sabarinathan, Prabharani Chaudhari, Pallavi Sinha, Vikas Kumar Singh, Sanghamitra Samantaray, Jawahar Lal Katara

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引用次数: 0

Abstract

Water availability is the most critical factor limiting rice yield in rainfed agro-ecosystems. Drought stress during the vegetative stage inhibits key growth processes, such as leaf formation and tillering, significantly impacting yield. This study aimed to investigate the genetic basis of vegetative stage drought tolerance and identify QTLs and genes associated with it through GWAS. A total of 19 major QTLs were identified for six traits: leaf rolling, relative water content, plant height, leaf area, tiller number, and leaf number, with phenotypic variances ranging from 10.55 to 80.05%. Additionally, haplotypes for six candidate genes were identified: OsCYP72A32 for leaf rolling, OsNCX5.2 for relative water content, OsSPX2 for plant height, OsSTA104 for tiller number, OsRING313 for leaf number and Os3BGlu6 for leaf area. Besides, genotypes such as NCS 901 A, H 15-23-DA, LOHAMBITRO and MEJANES 2 were found to be superior donors. These tolerant genotypes and superior haplotypes can be used in haplotype-based breeding programs to enhance drought tolerance in rice at vegetative stage.

Supplementary information: The online version contains supplementary material available at 10.1007/s12298-025-01573-7.

查看原文本刊更多论文

水稻营养期抗旱性优势单倍型的全基因组关联分析。

在雨养农业生态系统中，水分有效性是限制水稻产量的最关键因素。营养阶段的干旱胁迫抑制了关键的生长过程，如叶片形成和分蘖，显著影响产量。本研究旨在探讨植物营养期抗旱性的遗传基础，并通过GWAS鉴定与之相关的qtl和基因。在叶片卷曲、相对含水量、株高、叶面积、分蘖数和叶数6个性状上共鉴定出19个主要qtl，表型变异范围为10.55 ~ 80.05%。此外，还鉴定了6个候选基因的单倍型：叶片滚动基因OsCYP72A32、相对水分基因ossncx5.2、株高基因OsSPX2、分蘖数基因OsSTA104、叶数基因OsRING313和叶面积基因Os3BGlu6。此外，NCS 901 A、h15 -23- da、LOHAMBITRO和MEJANES 2等基因型是较好的供体。这些耐旱性基因型和优势单倍型可用于单倍型育种计划，以提高水稻营养期的耐旱性。补充信息：在线版本包含补充资料，可在10.1007/s12298-025-01573-7获得。

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来源期刊

Physiology and Molecular Biology of Plants PLANT SCIENCES-

CiteScore

7.10

自引率

0.00%

发文量

126

期刊介绍： Founded in 1995, Physiology and Molecular Biology of Plants (PMBP) is a peer reviewed monthly journal co-published by Springer Nature. It contains research and review articles, short communications, commentaries, book reviews etc., in all areas of functional plant biology including, but not limited to plant physiology, biochemistry, molecular genetics, molecular pathology, biophysics, cell and molecular biology, genetics, genomics and bioinformatics. Its integrated and interdisciplinary approach reflects the global growth trajectories in functional plant biology, attracting authors/editors/reviewers from over 98 countries.