Physiology and Molecular Biology of Plants最新文献

{"title":"Isolation and evaluation of growth-promoting endophytic bacteria from <i>Zanthoxylum dissitum</i> Hemsl.","authors":"Lingyu Fei, Ziying Hou, Yuan Wang, Jikang Sun, Tingting An, Qiuyun Li","doi":"10.1007/s12298-025-01552-y","DOIUrl":"10.1007/s12298-025-01552-y","url":null,"abstract":"<p><p>The slow growth rate of <i>Zanthoxylum dissitum</i> Hemsl. (Zanthoxylum) is the important factor causing the scarcity of its available wild resource. It has been reported that the plant endophytes can promote the plant growth and the synthesis of secondary metabolitesby by enhancing the efficiency of nutrient absorption by plants and regulating plant hormones. It is important to explore the promoting effects of endophytes on the growth of Zanthoxylum. The application of high-throughput sequencing technology in this study revealed the presence of three phyla, five classes, seven orders, and eleven genera of endophytic bacteria in Zanthoxylum. The most prevalent phyla, classes, orders, and genera were identified respectively as Proteobacteria, Gammaproteobacteria, Burkholderiales, and Pseudomonas. In this study, an endophytic growth-promoting bacterium was isolated and identified as <i>Sphingomonas</i> sp. The results revealed that the bacterium exhibited robust nitrogen fixation, phosphorus solubilization, and effective siderophore production capabilities. The phosphate solubilization index (SI) was found to be (1.266 ± 0.0157). Following a 48-h incubation period in an inorganic phosphorus liquid medium (PKO), the concentration of auxin (IAA) and gibberellin (GA) reached their highest levels, at (138.145 ± 65.111) μg/mL and (805.74 ± 123.86) μg/mL, respectively. Moreover, the study showed that the endophytic bacteria markedly enhanced the germination potential and rate of sorghum seeds, and promoted significantly the growth of the tissue culture seedlings of Zanthoxylum.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 2","pages":"299-310"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11890836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Opportunities and challenges in the application of single-cell transcriptomics in plant tissue research.","authors":"Man Luo, Yunpeng Cao, Jiayi Hong","doi":"10.1007/s12298-025-01558-6","DOIUrl":"10.1007/s12298-025-01558-6","url":null,"abstract":"<p><p>Single-cell transcriptomics overcomes the limitations of conventional transcriptome methods by isolating and sequencing RNA from individual cells, thus capturing unique expression values for each cell. This technology allows unprecedented precision in observing the stochasticity and heterogeneity of gene expression within cells. However, single-cell RNA sequencing (scRNA-seq) experiments often fail to capture all cells and genes comprehensively, and single-modality data is insufficient to explain cell states and systemic changes. To address this, the integration of multi-source scRNA-seq and single-cell multi-modality data has emerged, enabling the construction of comprehensive cell atlases. These integration methods also facilitate the exploration of causal relationships and gene regulatory mechanisms across different modalities. This review summarizes the fundamental principles, applications, and value of these integration methods in revealing biological changes, and analyzes the advantages, disadvantages, and future directions of current approaches.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 2","pages":"199-209"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11890805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive in-silico characterization and expression pattern of calmodulin genes under various abiotic and biotic stresses in Indian mustard (<i>Brassica juncea</i>).","authors":"Prashasti Pandey, Garima Rai, Anchal Garg, Deepak Kumar","doi":"10.1007/s12298-025-01561-x","DOIUrl":"10.1007/s12298-025-01561-x","url":null,"abstract":"<p><p>Calcium (Ca²⁺) as a secondary messenger has a multidimensional role, including the growth and development of plants and the adaptive response to stress conditions. Calmodulin (CaM), a calcium-binding protein, uniquely binds with these Ca²⁺ ions and transmits Ca²⁺ signals. Calmodulin proteins have been well-reported in various plants for playing a role in abiotic and biotic stress signaling; however, a comprehensive analysis of the <i>CaM</i> genes of Indian mustard (<i>Brassica juncea</i>) has not been studied much. This study reports their chromosome placements, phylogenetic relations, the presence of protein motifs and <i>cis</i>-acting elements, and their expression patterns under stress due to salt, heat, cadmium, <i>Xanthomonas campestris,</i> and <i>Alternaria brassicae</i>. We identified 23 <i>BjCaM</i> genes coding for eight BjCaM proteins possessing the signature EF-hand domains. Chromosome locations, intron-exon structure, and <i>in-silico</i> protein characterization pointed toward genetic diversification. Phylogenetic analysis revealed a close relationship with previously characterized CaM proteins from <i>Arabidopsis</i> and rice. <i>Cis</i>-acting elements in the promoter regions suggested the potential role of BjCaM candidates in hormone signaling and various stress-responsive regulatory mechanisms. qRT-PCR analysis showed differential expression patterns, of which <i>BjCaM17</i> and <i>BjCaM19</i> showed higher expression under all stresses. The seven selected <i>BjCaM</i> genes were sensitive to cadmium stress. Interestingly, despite translating to same protein, <i>BjCaM15, BjCaM17</i>, and <i>BjCaM19</i> showed differential expressions under the same stresses. This research represents the first genome-wide analysis of calmodulin genes in Indian mustard, providing a valuable reference for decoding calcium signaling via calmodulin and its potential exploitation to improve crop resistance to stress conditions.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01561-x.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 2","pages":"247-262"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11890825/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variable infection mechanisms of mungbean yellow mosaic India virus in diverse <i>Vigna</i> species: New insights from differential gene expression.","authors":"Kuppuraj Jagadeesan, Nagendran Krishnan, Asmita Sirari, Bharathi Mohindru, Manmohan Dhkal","doi":"10.1007/s12298-025-01547-9","DOIUrl":"10.1007/s12298-025-01547-9","url":null,"abstract":"<p><p>The extent of viral infection significantly shapes disease susceptibility. Yellow mosaic disease induced by the begomovirus pathogen mungbean yellow mosaic India virus (MYMIV), revealed varying infection levels in both compatible and incompatible interactions across three distinct <i>Vigna</i> species such black gram, green gram, and rice bean. Differential gene expression analysis focused on MYMIV coat protein (<i>AV1</i>) and replication protein (<i>AC1</i>) highlighted elevated <i>AV1</i> expression in the susceptible green gram genotype SML1082 compared to the black gram genotype KUG253. Conversely, <i>AC1</i> showed higher expression in black gram than green gram, illustrating complex infection mechanisms among compatible MYMIV-<i>Vigna</i> interactions. A novel infection pathway, termed \"Lack of Efficient Assembly (LEA),\" has been hypothesized in MYMIV-<i>Vigna</i> interactions. Additionally, a whitefly-mediated artificial transmission model for begomoviruses, named Transparent Airflow Stress-free Container (TASC), has been designed and demonstrated for the efficient transmission of MYMIV. This study enhances the understanding of begomovirus infection dynamics in diverse <i>Vigna</i> species, offering insights into disease management strategies.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01547-9.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 1","pages":"153-162"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787106/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>SAL1</i> gene: a promising target for improving abiotic stress tolerance in plants a mini review.","authors":"Hany Elsharawy, Moath Refat","doi":"10.1007/s12298-025-01549-7","DOIUrl":"10.1007/s12298-025-01549-7","url":null,"abstract":"<p><p>Global climate change poses a significant risk to agricultural productivity due to its diverse impacts on agricultural ecosystems, such as increased temperatures and altered precipitation patterns, all of which can adversely affect crop productivity. To overcome these challenges, plants have evolved intricate mechanisms to regulate stress responses and enhance stress tolerance. The <i>SAL1</i> gene, which encodes a phosphatase enzyme, has emerged as a key player in plant stress responses. In this review, we provide an overview of the <i>SAL1</i> gene, its functional significance, and its potential applications for improving stress tolerance in crops. To address the escalating global food demand amidst climate change challenges, it is imperative to pursue innovative strategies aimed at enhancing crop tolerance against abiotic stress.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 1","pages":"1-9"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787127/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geminivirus diseases of legumes in India: current status and approaches for management.","authors":"Sanjeev Kumar, Anurabh Chakravarty, Lingaraj Sahoo","doi":"10.1007/s12298-024-01531-9","DOIUrl":"10.1007/s12298-024-01531-9","url":null,"abstract":"<p><p>India has a large potential for producing a variety of legumes which are proficiently valued for small grower to the highest producers. Plant viruses predominate among the many factors that affect the production of legumes. In tropical and subtropical locations, begomovirus has become a significant productivity barrier for legume production with significant losses. The detection and molecular characterization of various begomoviruses species have been done with regard to phylogenetic analyses, infectivity on host plants, DNA replication, transgenic resistance, promoter analysis, and development of virus-based gene-silencing vectors using several techniques. The molecular detection of begomoviruses involves a variety of techniques, including polymerase chain reaction (PCR), using degenerate primers, reverse transcription PCR (RT-PCR), real time quantitative PCR, rolling-circle amplification PCR (RCA-PCR assay), RCA, and microarray/DNA chip. Begomovirus infections can be prevented by various methods such as by controlling vector populations, use of culture practices, developing virus-free planting materials, developing resistant varieties, following quarantine regulations, and adapting modern methods, including pathogen-derived resistance (PDR), RNA interference (RNAi)-mediated resistance and genome editing approach. This review focuses on current status of geminiviruses infecting various legumes, pathogenesis, genetic flexibility, recombination of begomovirus responsible for the wide host range, modern methods of control, including PDR, RNAi-mediated resistance, small RNA (sRNA)- mediated resistance, Engineered Nucleases, Zinc Finger nucleases, Transcriptional Activator nucleases, CRISPR/Cas9 mediated genome editing and various strategies for management of begomoviruses. The present study entails the view and understanding of different approaches for the begomovirus management which state knowledge about limiting the crop losses.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 1","pages":"41-65"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787143/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial miRNAs and target-mimics as potential tools for crop improvement.","authors":"Tilahun Rabuma, Neeti Sanan-Mishra","doi":"10.1007/s12298-025-01550-0","DOIUrl":"10.1007/s12298-025-01550-0","url":null,"abstract":"<p><p>MicroRNAs (miRNAs) are endogenous, small molecules that negatively regulate gene expression to control the normal development and stress response in plants. They mediate epigenetic changes and regulate gene expression at both transcriptional and post-transcriptional levels. Synthetic biology approaches have been utilized to design efficient artificial miRNAs (amiRNAs) or target-mimics to regulate specific gene expression for understanding the biological function of genes and crop improvement. The amiRNA based gene silencing is an effective technique to \"turn off\" gene expression, while miRNA target-mimics or decoys are used for efficiently down regulating miRNAs and \"turn on\" gene expression. In this context, the development of endogenous target-mimics (eTMs) and short tandem target mimics (STTMs) represent promising biotechnological tools for enhancing crop traits like stress tolerance and disease resistance. Through this review, we present the recent developments in understanding plant miRNA biogenesis, which is utilized for the efficient design and development of amiRNAs. This is important to incorporate the artificially synthesized miRNAs as internal components and utilizing miRNA biogenesis pathways for the programming of synthetic circuits to improve crop tolerance to various abiotic and biotic stress factors. The review also examines the recent developments in the use of miRNA target-mimics or decoys for efficiently down regulating miRNAs for trait improvement. A perspective analysis and challenges on the use of amiRNAs and STTM as potent tools to engineer useful traits in plants have also been presented.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 1","pages":"67-91"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787108/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of biogenic zinc oxide nanoparticles on physiological and biochemical attributes of pea (<i>Pisum sativum</i> L.) under drought stress.","authors":"Aneeza Ishfaq, Irfan Haidri, Usman Shafqat, Imran Khan, Muhammad Iqbal, Faisal Mahmood, Muhammad Umair Hassan","doi":"10.1007/s12298-024-01537-3","DOIUrl":"10.1007/s12298-024-01537-3","url":null,"abstract":"<p><p>Drought is a significant environmental issue affecting crop yield, nutrient content, and human food. This study investigates the potential of zinc oxide nanoparticles (ZnO-NPs) in mitigating the negative effects of drought stress on pea (<i>Pisum sativum</i> L.). ZnO-NPs were applied through seed priming, foliar application, and soil drenching at 0, 50, 100, and 150 ppm concentrations. Our findings showed that these three methods were more effective at different concentrations of ZnO-NPs. Seed priming at 50 ppm, foliar application at 100 ppm, and soil drenching at 150 ppm performed best in mitigating drought stress. Results showed that primed seeds with zinc oxide nanoparticles (50 ppm) have improved the physical growth, physiological, antioxidant, and mineral content by 35%, 45%, 57%, and 13% under drought stress as compared to control. It was observed that foliar application of ZnO-NPs (100 ppm) has enhanced physical growth, physiological, antioxidant, and mineral content by 43%, 54%, 64%, and 15% under drought stress as compared to the control. However, application of ZnO-NPs (150 ppm) in soli drenching improved the physical growth, physiological, antioxidant, and mineral content by 47%, 60%, 64%, and 16% under drought stress as compared to control. Moreover, ZnO-NPs amendments at different concentrations significantly decreased osmotic stress. This study provides innovative evidence of ZnO-NPs to mitigate drought stress in plants through various applications, revealing their potential to boost resilience in agriculture in case of drought stress conditions.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-024-01537-3.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 1","pages":"11-26"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marker assisted pyramiding of major resistance genes of tomato leaf curl and late blight diseases for stabilising tomato production.","authors":"Suresh Reddy Yerasu, H C Prasanna, Nagendran Krishnan, Sudarshan Maurya, Hukum Singh Panwar, Sudheer Kumar Reddy, Jagesh Kumar Tiwari, Nagendra Rai, Tusar Kanti Behera","doi":"10.1007/s12298-025-01548-8","DOIUrl":"10.1007/s12298-025-01548-8","url":null,"abstract":"<p><p>Globally<b>,</b> tomato leaf curl disease (ToLCD) caused by begomoviruses and late blight disease caused by <i>Phytophthora infestans</i> are important limiting factors for tomato production. Development of disease resistant cultivars is an important objective of tomato breeding programmes. Resistance genes such as <i>Ty2</i> and <i>Ty3</i> against ToLCD, and <i>Ph2</i> and <i>Ph3</i> resistant genes against late blight were utilized in this study to develop tomato lines with ToLCD and late blight resistance. Two F₂ populations derived from the crosses viz., VRT4-20-18 × (LA3152 × LA4286) and LA3152 × VRT-78-2 were used as base material. Marker assisted selection was employed throughout the generation advancement programme to select plants with the targeted genes. Gene based molecular markers AW910upF2R3, Ty3-SCAR and Ph3SCAR were used to select genotypes containing the <i>Ty2</i>, <i>Ty3</i> and <i>Ph3</i> genes, respectively. The dTG-63/<i>HinfI</i> CAPS marker linked to <i>Ph2</i> gene was used to select the <i>Ph2</i> gene containing genotypes. Advanced lines with all the four genes and/ or in different combinations with desirable agronomic traits were developed. Further, these selected genotypes were also tested for resistance to both the diseases under natural epiphytotic and artificially inoculated conditions. Lines with the <i>Ty-3</i> alone or in combination with the <i>Ty-2</i> performed well against ToLCD with DSI < 1, whereas lines with both the <i>Ph2</i> and <i>Ph3</i> genes performed well against late blight with DSI < 1. Developed advanced lines in the study can play a greater role in stabilizing tomato production by minimizing the losses caused by these diseases.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-025-01548-8.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 1","pages":"105-118"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787128/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of reverse transcription recombinase polymerase amplification assay for rapid diagnostics of Peanut mottle virus.","authors":"B Parameswari, P Anbazhagan, A Rajashree, G V Chaitra, Kavi Sidharthan, S K Mangrauthia, Faisal Yousuf, K Anitha, Y Prasanthi, B Bhaskar, V Celia Chalam, G P Singh","doi":"10.1007/s12298-024-01545-3","DOIUrl":"10.1007/s12298-024-01545-3","url":null,"abstract":"<p><p>Peanut mottle virus (PeMoV) is a single-stranded RNA virus transmitted through seeds and aphids that affects peanut crops worldwide. Currently, Enzyme Linked Immune-Sorbent assays and Reverse-Transcription Polymerase Chain Reaction techniques are widely employed to detect PeMoV in infected plants. ELISA is labor-intensive and time-consuming, as it involves the preparation of buffers and the production of polyclonal antibodies. Even though RT-PCR bypasses the need for buffer preparation and antibody production, it demands trained professional's manpower, requires expensive equipment like thermal cyclers, and involves complex procedures such as RNA isolation and cDNA conversion. To avoid these constraints, there is a need for a fast, reliable, efficient, and economical method for detecting PeMoV to ensure the production of healthy seeds. This study optimized the Reverse Transcriptase Recombinase Polymerase Amplification (RT-RPA) method by eliminating the steps of RNA extraction, cDNA conversion, and the use of a thermal cycler. The optimized RT-RPA assay successfully detected PeMoV at concentrations as low as 10^-6 and 10^-7 dilutions (1 and 0.1 µg/µl) of both RNA an-6d crude sap templates, demonstrating high sensitivity comparable to the routine RT-PCR assay. The new RT-RPA technique was tested against other viruses that infect peanuts like the Peanut stunt Virus, Tomato spotted wilt virus and Peanut bud necrosis virus, this technique demonstrated great specificity and no cross-reactivity. The developed RT-RPA using a crude leaf sap template is time-saving, less laborious, not very complicated, high specificity, sensitivity, economical and efficient. Therefore, laboratories with limited resources can use the RT-RPA assay for preliminary screening of PeMoV in nurseries, farm and glasshouse conditions, and quarantine stations. The current study reports the development, optimization and validation of Reverse Transcriptase Recombinase Polymerase Amplification (RT-RPA) using crude sap as template for the onsite detection of PeMoV infection in peanut crops under field conditions for the first time.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12298-024-01545-3.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"31 1","pages":"131-142"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}