Distinct immune pathways activated by harpin proteins in Sorghum bicolor.

IF 3.3 3区生物学 Q1 PLANT SCIENCES

Physiology and Molecular Biology of Plants Pub Date : 2025-05-01 Epub Date: 2025-06-03 DOI:10.1007/s12298-025-01601-6

Jaimini Patoliya, Khushali Thaker, Khushbu Rabadiya, Jalpa Patel, Tithi Trivedi, Saumya Patel, Nayan Jain, Prasant Kumar, Rushikesh Joshi

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引用次数: 0

Abstract

This study investigates how two harpin proteins, HrpZ and Hpa1, modulate plant immunity as potential alternatives to pesticides. Despite the established importance of SA in immunity, its regulatory complexity and gene expression dynamics are partially elucidated. We examined the effect of HrpZ and Hpa1 on the SA signalling pathway and the expression of seven marker genes (NPR1, EDS1, CAD1, NSL1, AED3, AED4, WRKY8) identified through existing microarray dataset analysis and analysed by qRT-PCR and HPLC. Our findings reveal distinct modes of action: HrpZ strongly activates SA-mediated immunity by upregulating SA levels and SA-responsive genes. Conversely, Hpa1 does not significantly impact SA levels but upregulates AED3 and AED4, suggesting an alternative, SA-independent defence mechanism. This research provides valuable insights into harpin-induced plant immunity, paving the way for targeted biostimulant development in sustainable agriculture.

Supplementary information: The online version contains supplementary material available at 10.1007/s12298-025-01601-6.

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高粱双色蛋白激活的不同免疫途径。

本研究探讨了两种harpin蛋白HrpZ和Hpa1作为杀虫剂的潜在替代品如何调节植物免疫。尽管SA在免疫中的重要性已经确立，但其调控复杂性和基因表达动力学尚不完全清楚。我们检测了HrpZ和Hpa1对SA信号通路和7个标记基因（NPR1、EDS1、CAD1、NSL1、AED3、AED4、WRKY8）表达的影响，这些基因通过现有的微阵列数据集分析，并通过qRT-PCR和HPLC分析。我们的发现揭示了不同的作用模式：HrpZ通过上调SA水平和SA应答基因强烈激活SA介导的免疫。相反，Hpa1不显著影响SA水平，但上调AED3和AED4，提示另一种不依赖于SA的防御机制。该研究为harpin诱导的植物免疫提供了有价值的见解，为可持续农业中靶向生物刺激素的开发铺平了道路。补充信息：在线版本包含补充资料，可在10.1007/s12298-025-01601-6获得。

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来源期刊

Physiology and Molecular Biology of Plants PLANT SCIENCES-

CiteScore

7.10

自引率

0.00%

发文量

126

期刊介绍： Founded in 1995, Physiology and Molecular Biology of Plants (PMBP) is a peer reviewed monthly journal co-published by Springer Nature. It contains research and review articles, short communications, commentaries, book reviews etc., in all areas of functional plant biology including, but not limited to plant physiology, biochemistry, molecular genetics, molecular pathology, biophysics, cell and molecular biology, genetics, genomics and bioinformatics. Its integrated and interdisciplinary approach reflects the global growth trajectories in functional plant biology, attracting authors/editors/reviewers from over 98 countries.