Study of genetic variability and emerging strains of Okra enation leaf curl virus: increasing risks to okra production in India.

IF 3.3 3区生物学 Q1 PLANT SCIENCES

Physiology and Molecular Biology of Plants Pub Date : 2025-05-01 Epub Date: 2025-04-11 DOI:10.1007/s12298-025-01578-2

Ankit Kumar, Jyoti Singh, Anupma Singh, Dharmendra Pratap

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引用次数: 0

Abstract

Okra enation leaf curl disease (OELCuD), caused by Okra enation leaf curl virus (OELCuV) and transmitted by whiteflies (Bemisia tabaci), significantly threatens okra cultivation in India. This study conducted a comprehensive survey (2020-2022) across seven Indian states, recording disease incidence ranging from 14.03% to 67.57%. Polymerase chain reaction (PCR) using coat protein gene-specific primers confirmed the presence of OELCuV in symptomatic plants, amplifying a ~ 750 bp fragment. Full-genome characterization of five isolates from different geographic regions using rolling circle amplification (RCA) revealed high genetic variability, with nucleotide identities ranging from 92.2% to 96.5% compared to existing OELCuV DNA-A sequences. Two novel strains were identified in Meerut (Uttar Pradesh) and Viluppuram (Tamil Nadu), meeting the International Committee on Taxonomy of Viruses (ICTV) species demarcation criteria. Phylogenetic and recombination analyses demonstrated that these strains form a unique cluster with recombinant features, particularly in the AC1 coding region, which is under purifying selection. The findings underscore the urgent need to monitor the genetic variability and spread of OELCuV to protect okra cultivation from evolving viral threats.

Supplementary information: The online version contains supplementary material available at 10.1007/s12298-025-01578-2.

查看原文本刊更多论文

秋葵发生卷曲叶病毒的遗传变异和新毒株研究：增加印度秋葵生产的风险。

秋葵成卷叶病（OELCuD）由秋葵成卷叶病毒（OELCuV）引起，由白蝇（烟粉虱）传播，严重威胁着印度秋葵的种植。该研究对印度7个邦进行了全面调查（2020-2022年），记录了14.03%至67.57%的疾病发病率。利用外壳蛋白基因特异性引物进行聚合酶链反应（PCR），扩增了约750 bp的片段，证实了OELCuV在有症状植物中的存在。利用滚环扩增技术（RCA）对来自不同地理区域的5株OELCuV进行全基因组鉴定，发现与现有OELCuV DNA-A序列相比，其核苷酸同源性在92.2%至96.5%之间。在Meerut （Uttar Pradesh）和Viluppuram （Tamil Nadu）发现了两种新毒株，符合国际病毒分类学委员会（ICTV）的物种划分标准。系统发育和重组分析表明，这些菌株形成了一个独特的具有重组特征的集群，特别是在AC1编码区，该区域处于纯化选择状态。这些发现强调了迫切需要监测OELCuV的遗传变异和传播，以保护秋葵种植免受不断进化的病毒威胁。补充信息：在线版本包含补充资料，可在10.1007/s12298-025-01578-2获得。

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来源期刊

Physiology and Molecular Biology of Plants PLANT SCIENCES-

CiteScore

7.10

自引率

0.00%

发文量

126

期刊介绍： Founded in 1995, Physiology and Molecular Biology of Plants (PMBP) is a peer reviewed monthly journal co-published by Springer Nature. It contains research and review articles, short communications, commentaries, book reviews etc., in all areas of functional plant biology including, but not limited to plant physiology, biochemistry, molecular genetics, molecular pathology, biophysics, cell and molecular biology, genetics, genomics and bioinformatics. Its integrated and interdisciplinary approach reflects the global growth trajectories in functional plant biology, attracting authors/editors/reviewers from over 98 countries.