Planta medica最新文献

{"title":"Ginsenoside Rd Ameliorates Glucose Metabolism by Regulating GLP-1/MAPK/NF-κB Signaling Pathway in db/db Mice.","authors":"Wenbin Wu, Yujin Guo, Qingsong Xia, Hongzhan Wang, Hui Dong, Fuer Lu, Chenghong Zheng, Fen Yuan","doi":"10.1055/a-2898-9988","DOIUrl":"10.1055/a-2898-9988","url":null,"abstract":"<p><strong>Abstract: </strong>Ginsenoside Rd shows positive effects on T2DM, but its mechanism remains unclear. In this study, we explored its mechanism focusing on inflammation levels in the pancreas and intestine, and provided evidence that this is related to modulation of the GLP-1/MAPK/NF-<i>κ</i>B signaling pathway. We recorded and compared changes in metabolic indicators of T2DM, and tested levels of GLP-1 and GLP-1R through immunohistochemistry, Western blotting, and PCR after the mice were euthanized. Additionally, we detected the effects of ginsenoside Rd on inflammatory related molecules such as AMPK, Sirt1, MAPK, and NF-<i>κ</i>B by ELISA, transcriptomics, Western blot, and PCR, while also conducting in vitro experiments to investigate the GLP-1 signaling pathway. After treatment with ginsenoside Rd, blood glucose decreased and insulin resistance weakened in db/db mice, and the results showed an increase in the expression of GLP-1 and GLP-1R. Transcriptome analysis revealed differences in inflammatory response-related signaling pathways after treatment, and Western blot results showed increased AMPK phosphorylation and decreased MAPK phosphorylation. Consistent experimental results were also obtained in vitro. In conclusion, our data suggest that ginsenoside Rd may ameliorate T2DM in db/db mice, potentially through the inhibition of inflammatory responses in the intestine and pancreas, an effect that might be mediated by the promotion of GLP-1 secretion.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148284091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Serratane Triterpenoid and Musashi2 inhibitors from Huperzia serrata.","authors":"Tamara Balsiger, Malik Rakhmanov, Alessandro Prescimone, Annalena Kaiser, Kim-Dung Huynh, Andreas Papassotiropoulos, Robin Teufel, Attila Stetak, Eliane Garo","doi":"10.1055/a-2900-3049","DOIUrl":"https://doi.org/10.1055/a-2900-3049","url":null,"abstract":"<p><strong>Abstract: </strong>Forgetting and memory formation are 2 distinct processes, both essential for the survival of humans and other animals. The protein Musashi2 (MSI2) was recently discovered as a key regulator of forgetting in the model organism <i>Caenorhabditis elegans</i>, making it an attractive therapeutic target for drug discovery and development.</p><p><strong>Abstract: </strong>Here, we report a fluorescence polarization assay-based screening campaign aimed at identifying MSI2 inhibitors from 2,844 plant extracts. The dichloromethane extract of the aerial parts of <i>Huperzia serrata</i>, a plant traditionally used in Chinese medicine for the treatment of memory dysfunction, was identified as a hit and subsequently subjected to HPLC-based activity profiling. Scale-up isolation identified fatty acids, namely linoleic acid (11: ) and palmitoleic acid (12: ), both eluting within the main active window. As other fractions also seem to display activity, although weaker, their minor constituents were also investigated, revealing 1 new serratane triterpenoid (1: ) and 7 known serratene triterpenoids 2: -8: , their biosynthetic precursor <i>α</i>-onocerin (9: ), as well as an abietane diterpene (10: ). The absolute configuration of compound 1: was established by X-ray crystallography. All isolated compounds were evaluated for their activity, revealing that linoleic acid (11: ) and palmitoleic acid (12: ) inhibited MSI2 with IC₅₀ values of 14.6 and 23.1 µM, respectively, while compound 10: only showed moderate activity (IC₅₀ of 34.4 µM).</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148397443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leveraging Biochemometrics to Prioritize Biologically Relevant Compounds from Natural Product Mixtures: A Journey from Conception to Successful Outcomes.","authors":"Prateek Singh Bora, Upendra Sharma","doi":"10.1055/a-2893-0341","DOIUrl":"https://doi.org/10.1055/a-2893-0341","url":null,"abstract":"<p><strong>Abstract: </strong>Biochemometrics has evolved as a powerful tool in natural product research that enables the simultaneous identification of reliable putative bioactive compounds, while offering insights into the chemical composition of complex natural product extracts. The approach has steadily evolved from its initial phase with the advent of sophisticated and highly sensitive MS- and NMR-based analytical techniques, and has greatly impacted natural product drug discovery pipelines by complementing bioassay-guided fractionation. It has proven effective in identifying active compounds within natural product mixtures and investigating synergistic effects among constituents. Integrating biochemometrics with dereplication and compound annotation approaches via GNPS-based molecular networking has accelerated the discovery of novel chemical scaffolds with biological potential. This review offers a chronological assessment of biochemometrics in natural product research, critically examining its evolution from the inception of the concept, development in the field, to its present applications in natural product research. It outlines its diverse applications across plant, microbial, and marine natural product sources, highlighting key case studies that demonstrate its utility across different natural product sources for the identification of bioactive compounds. By evaluating its strengths and limitations, this review underscores the critical importance of rigorous data acquisition and preprocessing as critical steps inherent to the success of biochemometric modeling. Model validation is considered equally important to ensure the robustness and accuracy of the derived projections. As omics continue to advance with sophisticated analytical and computational techniques, the review also identifies emerging opportunities for integrating biochemometrics with GNPS-based molecular networking, and employing dereplication via NP databases and artificial intelligence. These integrated approaches enhance predictive modeling and pattern recognition, enable dereplication with global natural product databases, and streamline prioritization of bioactive natural products. Together, the biochemometrics and its advancements will accelerate the discovery of novel bioactive natural products and support the development of next-generation natural product-based therapeutic drugs.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148397314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemicals to Pharmacology: Potential Cytotoxic and Tumor Inhibitory Activity of Elaeocarpus ganitrus Extract in Preclinical Models.","authors":"Prashant Kumar Pandey, Milind Sagar, Shiva Sharma, Manisha Rastogi","doi":"10.1055/a-2899-3112","DOIUrl":"https://doi.org/10.1055/a-2899-3112","url":null,"abstract":"<p><strong>Abstract: </strong><i>Elaeocarpus ganitrus</i>, popularly known as Rudraksha, is of immense holistic importance in traditional Indian culture with spiritual and medicinal benefits. The present study aims to characterize and explore the cytotoxic and tumor inhibitory potential of hydroalcoholic extract of Rudraksha beads in preclinical models. Hydroalcoholic extract was prepared from powdered Rudraksha beads and was characterized through qualitative and quantitative phytochemical analysis, FT-IR, and LC-MS/MS analysis. <i>In vitro</i> cytotoxic activity of Rudraksha bead extract was evaluated in PC3, U87MG, A549, HCT116, FaDu, MCF-7, and LLC1 cell lines by MTT assay. Apoptotic activity was assessed by Western blot analysis of Bax, Caspase-3, and Cleaved Caspase-3 proteins in the HCT116 cell line. <i>In vivo</i> antitumor efficacy was evaluated in the LLC1 allograft and HCT116 xenograft mouse model. Hydroalcoholic extract of Rudraksha bead showed abundance of polyphenolic compounds and their subtypes like phenols, flavonoids, and anthocyanins in different analytical techniques. Treatment with hydroalcoholic extract elicited a dose-dependent decrease in cell viability in all the tested cell lines, with the best response in HCT116 (CC50 = 47.22, 95% confidence interval (CI): 42.75 - 49.06 µg/mL), with upregulated expression of pro-apoptotic markers, Caspase-3 (1.53-fold), Cleaved Caspase-3 (1.47-fold), and Bax (6.4-fold). Treatment with 500 mg/kg of Rudraksha extract in the LLC1 tumor allograft model (QD × 9 days) and HCT116 xenograft model (QD × 21 days) demonstrated significantly reduced tumor volume in comparison to the vehicle control. Hydroalcoholic extract of Rudraksha demonstrated a promising tumor inhibition response in preclinical settings, therefore making it a potential therapeutic candidate for cancer treatment.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148376543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and Safety of Ivy Leaf Dry Extract EA 575 in Cough Management: An Updated Review of Clinical Evidence.","authors":"Inga Trompetter, Simon Braun","doi":"10.1055/a-2745-1130","DOIUrl":"10.1055/a-2745-1130","url":null,"abstract":"<p><strong>Abstract: </strong>EA 575, the ivy leaf dry extract of the herbal medicine Prospan, has been widely used for the management of cough associated with various respiratory conditions. Over the past decade, new evidence has emerged from interventional and non-interventional studies offering a more comprehensive understanding of its efficacy, safety, and tolerability. This review provides an updated synthesis of clinical evidence of EA 575 in pediatric and adult patients with respiratory disease. A total of 27 publications were included, covering 13 interventional trials, 13 non-interventional studies, and 1 meta-analysis. These studies collectively enrolled 102 239 patients, of whom 84 022 received EA 575 treatment. The findings consistently demonstrate that EA 575 improves cough symptoms and lung function, as shown by improvements in the Bronchitis Severity Score, spirometry, and body plethysmography, depending on the parameters that have been assessed. Moreover, the tolerability of EA 575 has been increasingly confirmed through large real-world evidence studies that report a low number of non-serious adverse events. These results reinforce the well-established role of EA 575 in the effective and safe treatment of cough in clinical practice.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"774-789"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13349513/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146041257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication and Evaluation of 3D-printed Dittany-infused Patches for Anti-inflammatory Dermatological Applications.","authors":"Charikleia Paloukopoulou, Konstantina Chachlioutaki, Aliki Papadimitriou-Tsantarliotou, Savvas Koltsakidis, Dimitrios Tzetzis, Ioannis S Vizirianakis, Efstratios Vakirlis, Dimitrios G Fatouros, Anastasia Karioti","doi":"10.1055/a-2809-7446","DOIUrl":"10.1055/a-2809-7446","url":null,"abstract":"<p><strong>Abstract: </strong>This study aimed to validate the dermatological potential of <i>Origanum dictamnus</i> (dittany) infusion, traditionally recognized for its anti-inflammatory properties. To this end, 3D-printed hydrogel patches incorporating dittany were developed and evaluated for biocompatibility, wound-healing, anti-inflammatory activity, and effects on skin barrier. Hydrogel patches were fabricated via extrusion-based 3D printing using aloe vera gel, hydroxypropyl methylcellulose (HPMC), glycerol, and combinations of dittany infusion and panthenol. <i>In vitro</i> assays with HaCaT keratinocytes included cytotoxicity testing, scratch-wound healing, and qPCR-based analysis of inflammatory biomarkers. <i>In vivo</i> transepidermal water loss (TEWL) measurements were performed to assess skin barrier integrity and hydration after patch application. The traditional use of dittany infusion in skin ailments was confirmed, along with its relatively low cytotoxicity. Three 3D-printed gel types were evaluated <i>in vitro</i>, all showing superior performance to the raw plant extract. The optimal formulation contained 2% HPMC, 2% glycerol, 5% panthenol, and 0.06% dittany infusion (w/w% relative to aloe vera gel). At 3.25 mg/mL, this formulation achieved ~ 70% wound closure and significantly reduced IL-6 and TNF-<i>α</i> levels, indicating strong anti-inflammatory activity (p < 0.05). Both crude extract and patches show a trend toward reducing pro-inflammatory interleukins while simultaneously upregulating the anti-inflammatory cytokine IL-4. TEWL measurements indicated that none of the patches impaired the skin barrier function, confirming their compatibility with various skin types. This study provides the first scientific evidence that the incorporation of dittany infusion into 3D-printed hydrogels holds promise for safe, effective, plant-based topical therapies.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"824-834"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147309104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hormonal Modulation with Withania somnifera: Systematic Review and Meta-Analysis of Randomized-controlled Trials.","authors":"Michal Fornalik, Anna Malkiewicz, Dominika Adamczak, Filip Nawrocki, Aleksandra Zielinska, Samim Ali Mondal","doi":"10.1055/a-2802-8363","DOIUrl":"10.1055/a-2802-8363","url":null,"abstract":"<p><strong>Abstract: </strong>Hormonal imbalances, particularly involving the hypothalamic-pituitary-adrenal and gonadal axes, contribute to chronic conditions such as stress-related disorders, infertility, and metabolic dysregulation. Ashwagandha (<i>Withania somnifera L</i>.) has been proposed as a potential oral modulator of endocrine function due to its adaptogenic properties. This review aims to systematically evaluate the effects of oral ashwagandha supplementation on circulating hormone levels in adults. Our literature search included MEDLINE, Embase, and ClinicalTrials.gov. The protocol was registered in PROSPERO (CRD42024611576). We included randomized, placebo-controlled trials in adults assessing oral ashwagandha versus placebo that reported changes in hormone levels. Two reviewers independently screened studies, extracted summary data, and assessed risk of bias using the Cochrane tool. Meta-analyses were conducted using a random-effects model to calculate mean differences (MD) or standardized mean differences (SMD), with heterogeneity and publication bias assessed using I² and Egger's test, respectively. Twenty-three trials were included, encompassing 1706 patients. Ashwagandha significantly reduced cortisol (SMD =- 1.18, p < 0.04) and increased serotonin (MD = 31.75 ng/ml, p < 0.01). Subgroup analyses suggested possible dose-response effects based on withanolide content. No effect was found on TSH or T3, but T4 levels increased modestly (MD = 0.61 µg/dL, p = 0.02). Ashwagandha increased testosterone in men (MD = 57.43 ng/dl) but not in women (MD = 5.09 ng/dl), with a significant gender difference. This treatment did not influence E2 levels in either gender. Ashwagandha supplementation appears to modulate key hormonal pathways, particularly cortisol and sex hormones. However, heterogeneity and limited data on some endpoints warrant further standardized trials.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"790-805"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147309165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dioscorea bulbifera: Phytotherapeutic Potential and Toxicological Risks, A Critical Review.","authors":"Emi Mathew, Philip Litto Thomas, Linu Mathew","doi":"10.1055/a-2779-6570","DOIUrl":"10.1055/a-2779-6570","url":null,"abstract":"<p><strong>Abstract: </strong>Dioscorea bulbifera (air potato), a climbing perennial of the Dioscoreaceae family, is widely distributed in tropical regions and valued in Ayurveda, Traditional Chinese Medicine, and African ethnomedicine for managing inflammatory, infectious, metabolic, and neoplastic disorders. This systematic review critically evaluates its phytochemistry, pharmacology, and toxicology. Primary metabolites contribute to its nutritional value, whereas secondary metabolites-predominantly steroidal saponins, flavonoids, and diterpenoids from bulbils and tubers-underlie its wide range of reported bioactivities. African accessions are rich in clerodane diterpenoids, while Asian ones are rich in saponins and flavonoids, underscoring the need for comparative metabolomic and chemotaxonomic studies.</p><p><strong>Abstract: </strong>Preclinical studies confirm antiproliferative, antioxidant, anti-inflammatory, antimicrobial, antidiabetic, and neuroprotective effects. However, furanoid diterpenoids, particularly diosbulbin B and 8-epidiosbulbin E acetate, have been shown to induce hepatotoxicity, with additional risks of nephrotoxicity, cardiotoxicity, gastrointestinal irritation, and thyroid dysfunction. Traditional processing methods, such as boiling and roasting, as well as co-administration with protective herbs, mitigate toxicity. Despite promising activity, clinical translation remains hindered by phytochemical variability, non-standardised preparations, and the complete absence of human trials. Future research should therefore focus on developing detoxified, standardised extracts supported by pharmacokinetic studies and well-designed randomised controlled trials to establish D. bulbifera as a safe and effective phytotherapeutic agent.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"760-773"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145857541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesembryanthemum tortuosum and Zembrin: Mixed Evidence from In vivo Animal and Clinical Studies on their Antidepressant and Anxiolytic Effects.","authors":"Mika de Jong, Suzanne van Niekerk, Josias Hamman, Frank van der Kooy","doi":"10.1055/a-2809-5144","DOIUrl":"10.1055/a-2809-5144","url":null,"abstract":"<p><strong>Abstract: </strong><i>Mesembryanthemum tortuosum</i> is a succulent plant native to southern Africa and was traditionally used to enhance mood. Recent research into <i>M. tortuosum</i>'s mood-enhancing effects has culminated in the development of a standardised extract, Zembrin, containing consistent levels of mesembrine alkaloids considered to be the main bioactive compounds. This review aims to critically investigate published <i>in vivo</i> animal and clinical studies using <i>M. tortuosum</i>, Zembrin, and mesembrine alkaloids, to evaluate the study designs, formulations, and dosages used, and to assess the results in terms of safety and therapeutic efficacy as an antidepressant and anxiolytic. Four databases, namely PubMed, Scopus, Web of Science, and ScienceDirect, were searched using terms relating to 'clinical trials', '<i>in vivo</i>', '<i>Mesembryanthemum</i>', '<i>M. tortuosum</i>', 'pre-clinical', 'Sceletium', '<i>S. tortuosum</i>', and 'Zembrin'. The search was conducted to identify original research articles published before July 2025. Although published pre-clinical animal and clinical studies on <i>M. tortuosum-</i>derived products offer valuable insights, such as a favourable safety profile and potential efficacy as an anxiolytic and antidepressant, several limitations have been identified, including small sample sizes and studies conducted in clinically irrelevant populations. A limited number of reported outcomes suggest that Zembrin may have positive effects on anxiety, cognitive function, stress resilience, and mood. However, not all studies showed consistent benefits, and some outcomes were limited to specific measures such as EEG changes or reaction time under cognitive stress. Large-scale clinical trials in relevant populations should be conducted to determine the potential of <i>M. tortuosum</i>-derived products as anxiolytics and antidepressants.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"806-823"},"PeriodicalIF":2.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147344867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Study and Antibacterial Activity of Cedrus atlantica Aerial Part Apolar Extract.","authors":"Feryal Benayache, Valentina Parisi, Hamza Fadel, Giuliana Donadio, Michele D'Angiolo, Alessandra Braca, Nunziatina De Tommasi","doi":"10.1055/a-2896-2809","DOIUrl":"10.1055/a-2896-2809","url":null,"abstract":"<p><strong>Abstract: </strong>Phytochemical study of <i>Cedrus atlantica</i> aerial parts apolar extract led to the isolation of a new abietane diterpene (1: ), an abietane diterpene reported for the first time as a genuine natural product (2: ), 2 new megastigmane glycosides (3: -4: ), and 9 known compounds (5: -13: ), including 7 abietanes. Their structures were elucidated by 1D and 2D NMR spectroscopy and HRMS experiments. The crude extract was then evaluated through a broad screening against both Gram-positive and Gram-negative bacterial strains, with <i>Staphylococcus epidermidis</i> being the only affected microorganism. In a next step, a selection of pure compounds was also investigated, and the diterpene 15-hydroxydehydroabietic acid (15-HDAA) (11: ) was shown to exhibit detectable antibacterial activity against <i>S. epidermidis</i>. Growth kinetics analysis revealed a clear modulation of bacterial growth at sub-minimum inhibitory concentration (MIC), indicating a measurable physiological impact on the bacterium. This effect, when combined, allows a significant reduction in the effective concentration of ampicillin.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148258828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}