Planta medica最新文献

{"title":"Bioactive Metabolites from the Cultured Lichen Mycobionts of Astrothelium straminicolor and Nigrovothelium inspersotropicum.","authors":"Thi-Kim-Dung Le, Thi-Thanh-Van Ho, Thuc-Huy Duong, Ngoc-Hong Nguyen, Thi-Hoai-Thu Nguyen, Thi-Phuong Nguyen, Thi-Phi Giao Vo, Thanyada Rungrotmongkol, Chanat Aonbangkhen, Thi-Minh-Dinh Tran","doi":"10.1055/a-2855-3261","DOIUrl":"10.1055/a-2855-3261","url":null,"abstract":"<p><strong>Abstract: </strong>Cultivated lichen mycobionts represent a valuable resource for obtaining unique natural compounds. Mycobionts of <i>Astrothelium straminicolor</i> and <i>Nigrovothelium inspersotropicum</i>, collected in Vietnam, were isolated, cultivated, and subjected to chemical investigation. Consequently, three new compounds, straminones A - B (A1: and A2: ) and chlorosemivioxanthin (N1: ), along with five known compounds, 3<i>S</i>,4<i>R</i>-(+)-4-hydroxymellein (A3: ), 6-methoxymellein (A4: ), vioxanthin (N2: ), pigmentosin A (N3: ), and semivioxanthin (N4: ), were isolated and structurally elucidated. Straminones A and B represent the first examples of 3-methylhexahydrocyclopenta[<i>c</i>]pyran-1(3<i>H</i>)-one skeletons found in nature. The relative configuration of A1: was confirmed by DFT calculation. Compounds A3: and N4: demonstrated weak alpha-glucosidase inhibitory activity, with IC₅₀ values of 314 and 366 µM, respectively. The other compounds showed no inhibition in the tested assay. Notably, compounds N1: -N3: showed significant inhibitory effects on NO production, with IC₅₀ values ranging from 14 to 25 µM, compared with the positive control L-NMMA (IC₅₀ = 49 µM). Further molecular docking clarified the inhibitory mechanism of the active compounds.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"1060-1068"},"PeriodicalIF":2.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147932470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boldine and Laurolitsine Protect against Periodontitis-Associated Bone Loss via RANKL Downregulation.","authors":"Sebastián Castro-Saavedra, Gonzalo Fuentes-Barros, Carolina Rojas, Elizabeth Pellegrini, Michelle García, Marcela Hernández, Rolando Vernal, Bruce K Cassels","doi":"10.1055/a-2834-4446","DOIUrl":"10.1055/a-2834-4446","url":null,"abstract":"<p><strong>Abstract: </strong>Periodontitis is a chronic inflammatory disease driven by dysbiosis of the subgingival microbiota, leading to immune dysregulation and progressive alveolar bone loss. In recent years, immunotherapeutic approaches, particularly those involving natural products, have gained attention as potential adjuvants to conventional therapy. This study evaluated the effects of four alkaloids, boldine, laurolitsine, laurotetanine, and <i>N</i>-methyl-laurotetanine, on alveolar bone loss and local immune regulation in experimental periodontitis.</p><p><strong>Abstract: </strong>Male and female C57BL/6 mice with ligature-induced periodontitis received daily oral administration of each alkaloid (20 mg/kg) for 10 days. Alveolar bone loss was quantified by micro-computed tomography (micro-CT). Periodontal immune responses were assessed by RT-qPCR analysis of pro-inflammatory cytokines, T-cell-related transcription factors, and bone metabolism mediators (RANKL and OPG). In addition, regulatory T (Treg) cells in cervical lymph nodes were analyzed by flow cytometry.</p><p><strong>Abstract: </strong>Boldine and laurolitsine significantly reduced alveolar bone loss compared with untreated periodontitis, in parallel with decreased RANKL expression. All four alkaloids significantly reduced IL-6 expression, with laurolitsine additionally suppressing IL-17A expression in periodontal tissues, whereas <i>N</i>-methyl-laurotetanine reduced IL-10 expression. No significant changes were observed in CD4⁺, CD4⁺FoxP3⁺, and CD4⁺FoxP3⁺CD25⁺ T-cell frequencies between treated and untreated groups.</p><p><strong>Abstract: </strong>Oral administration of boldine and laurolitsine attenuates periodontitis-associated alveolar bone loss, likely through differential modulation of cytokine networks and osteoclastogenic signaling. These findings support their potential as host-modulating agents in the management of periodontitis.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"1030-1040"},"PeriodicalIF":2.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147469275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kynurenine and Related Metabolites in St. John's Wort (Hypericum perforatum).","authors":"Daniel Gaudet, Ryan Hayward, Pawanjit Kaur Sandhu, Susan J Murch, Lauren A E Erland","doi":"10.1055/a-2809-5487","DOIUrl":"10.1055/a-2809-5487","url":null,"abstract":"<p><strong>Abstract: </strong>Kynurenine (KYN) is a non-protein amino acid and major metabolite of tryptophan associated with human health. The acidic form, kynurenic acid (KYNA), has been previously identified in St. John's wort (SJW) tablets and tea, but other metabolites in the pathway are not known in the species. We developed and validated two liquid chromatography-mass spectrometry-based (UHPLC-Orbitrap MS) methods for the quantification of KYNA, KYN, 3-hydroxykynurenine (3-HK), and 3-hydroxyanthranilic acid (3-HAA). We compared two approaches: (1) derivatisation via 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) and (2) analysis of underivatised extracts. KYN was detected in both methods (78% and 101% recovery in underivatised and derivatised, respectively), and 3-HAA was detected only by the underivatised method (95%), while 3-HK was detected only by the derivatised method (90%). Methods were validated for key parameters, including linearity, accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ). Three of the KYN metabolites were detected in <i>in vitro</i>-grown SJW shoots: KYN (101 ng/g), KYNA (104 ng/g), and 3-HAA (670 ng/g). Significantly more KYN and KYNA was detected in greenhouse-grown plants than in tissue-cultured SJW. KYN, KYNA, and 3-HAA were also quantified in commercial preparations of SJW at 0.07 - 0.42 µg/g (KYN), 2.31 - 6.45 µg/g (KYNA), and 0.86 - 3.54 µg/g (3-HAA); 3-HK was not detected in any of our SJW samples. These data support future studies to understand the role of kynurenine metabolites in plant metabolism and plant-based products.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"969-979"},"PeriodicalIF":2.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147491484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant and Cytoprotective Role of Citral-Rich Essential Oils in In Vitro Animal Cell Models.","authors":"Brenna de Sousa Barbosa, Alexsandra Fernandes Pereira","doi":"10.1055/a-2809-5023","DOIUrl":"10.1055/a-2809-5023","url":null,"abstract":"<p><strong>Abstract: </strong>Citral-rich essential oils (CEO) exhibit remarkable antioxidant potential. In this review, we discuss chemical and biological aspects of citral and CEO, estimating possible mechanisms of action. To this end, a literature search was carried out in the Scielo, PubMed, and Google Scholar databases, focusing on the antioxidant potential of CEO. Analyzed studies demonstrated the protective capacity of citral and CEO in cells stressed by hydrogen peroxide and excess glucose in the medium. Moreover, citral has antimicrobial, anti-inflammatory, and antimutagenic activity that can be exploited in a cell culture. The antioxidant activity of citral is attributed to the presence of an aldehyde group and multiple unsaturations in its structure, which enable it to scavenge free radicals and convert them into auto-oxidizing epoxides that are rapidly eliminated by the cell. Citral epigenetically modulates DNA, increasing the activity of endogenous antioxidant enzymes, while inhibiting enzymes involved in lipid peroxidation. In parallel, CEO and citral contribute to maintaining DNA integrity through kinase-related signaling pathways. Despite growing evidence of their antioxidant potential, studies investigating the bioactivity of citral and CEOs in animal cell cultures remain limited. Furthermore, research exploring the synergistic activity of citral with other compounds is still scarce, despite their significant scientific relevance. This scenario reinforces the need for further investigations to elucidate their mechanisms of action and to establish reference parameters guiding their application in different cellular and reproductive models <i>in vitro</i>.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"840-853"},"PeriodicalIF":2.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147444624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Development of Immunological Quantification Method for Glycyrrhetinic Acid in Serum to Estimate the Potential Risk for Pseudoaldsteronism.","authors":"Shinsuke Hisaka, Yukina Ohno, Kazuko Takeda, Kyonosuke Takahashi, Mao Yano, Hayato Kishi, Sachiko Murase, Mitsuhiko Nose","doi":"10.1055/a-2802-8067","DOIUrl":"10.1055/a-2802-8067","url":null,"abstract":"<p><strong>Abstract: </strong>Glycyrrhizae Radix (GR), the dried root and stolon of <i>Glycyrrhiza uralensis</i> or <i>G. glabra</i>, is most frequently used as a crude drug, compounding Kampo medicines to treat a variety of diseases in Japan. This key crude drug, however, would be coinstantaneously a risk factor for pseudoaldosteronism. This adverse effect is thought to be due to glycyrrhetinic acid (GA), a major metabolite of glycyrrhizin, which is one of the ingredients of GR. Therefore, we thought that if the blood GA concentration could be measured quickly and easily when side effects occur, the causal relationship could be clarified, and side effects could be avoided. To realize this plan, we developed the competitive ELISA using anti-GA monoclonal antibody (mAb6D6), which was labeled 'biotin' to avoid endogenous effects, such as antibodies in serum, and to detect GA sensitively. This ELISA could quantify GA from about 10 pg/mL to 25 ng/mL. The quantitative value of GA in serum based on this ELISA was significantly corelated with that of GA based on high-performance liquid chromatography. Hence, the developed competitive ELISA could be an useful tool for users who would like to quantify the blood GA concentration to clarify the relationship between GA and pseudoaldosteronism derived from the intake of medicines containing GR and to lead to avoiding side effects derived from GA.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"963-968"},"PeriodicalIF":2.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147481374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Update on the Therapeutic Potential of Bixin and Norbixin Apocarotenoids.","authors":"Darío R Gómez-Linton, Adriana Monroy, Silvestre Alavez, Laura J Pérez-Flores","doi":"10.1055/a-2809-7329","DOIUrl":"10.1055/a-2809-7329","url":null,"abstract":"<p><strong>Abstract: </strong>Plants have been a central source of natural resources for humanity with all kinds of uses and applications: food, cosmetics, natural remedies and medicines, dyes, textiles, construction materials, ornamental uses, and the extraction of various compounds, including natural pigments. Within pigments, there are four major groups of interest: anthocyanins, chlorophylls, betalains, and carotenoids. Carotenoids are characterized by being formed by relatively long hydrocarbon chains (25 - 50 C), which can end in linear or cyclic structures. In the carotenoid group, bixin and norbixin are two compounds of undeniable relevance as food and cosmetic pigments. However, in recent years, these compounds have been studied to understand other possible effects, mainly in the field of health, yielding promising results. This review features results from studies published in recent years with a focus on possible applications in the field of public health, mainly related to metabolic problems such as sugar and fat metabolism. Additionally, various other health applications are analyzed, and the results are summarized. The main conclusions reached by the authors are also mentioned for a better understanding of the importance of each study. Finally, general conclusions and perspectives are provided in order to continue exploring the possible applications of bixin and norbixin as potential bioactive and functional compounds.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"945-962"},"PeriodicalIF":2.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147469225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Borassus aethiopum in Immunometabolic Pharmacology: Linking Phytochemistry, Oxidative Stress and Cytokine Signalling.","authors":"Deborah Bonifacio, Sofia Rapisarda, Roberto Genova, Guido Attilio Condorelli","doi":"10.1055/a-2859-4064","DOIUrl":"10.1055/a-2859-4064","url":null,"abstract":"<p><strong>Abstract: </strong><i>Borassus aethiopum</i>, a widely used West African palm, is a staple food source and a traditional medicinal plant with emerging relevance to immunometabolic pharmacology. This narrative review integrates ethnobotanical, phytochemical and pharmacological evidence, with particular emphasis on oxidative stress, cytokine signalling and low-grade inflammation. Available non-clinical and limited human data indicate that preparations derived from inflorescences, fruit pulp and kernels may exert antioxidant, anti-inflammatory and cardiometabolic effects, although the evidence base remains heterogeneous. Major constituents, including polyphenols, terpenoids, sterols, saponins and lipid fractions, are discussed in relation to redox-sensitive and inflammation-related pathways, particularly nuclear factor-kappa B (NF-<i>κ</i>B) and nuclear factor erythroid 2-related factor 2 (Nrf2), as well as cytokine networks implicated in metabolic and cardiovascular disorders. In rodent models, <i>B. aethiopum</i> preparations have been associated with reductions in oedema, nociception, pyrexia, hyperglycaemia and dyslipidaemia, often alongside lower C-Reactive protein and oxidative stress markers. Preliminary human studies using <i>B. aethiopum</i>-enriched bread in individuals at cardiometabolic risk have reported improvements in blood pressure (BP) and lipid profile, suggesting a possible effect on low-grade inflammatory status. Overall, the available evidence supports a multi-target framework in which <i>B. aethiopum</i> may influence cytokine-related and redox-regulated processes, while highlighting substantial limitations, including variable extraction methods, incomplete phytochemical characterisation, sparse cytokine measurements and small clinical samples. Future work should prioritise standardised chemo-fingerprinting, multiplex cytokine profiling, metabolomic integration and pragmatic dietary or extract-based interventions in at-risk populations. <i>B. aethiopum</i> may therefore serve as a useful model for a structured pharmacological evaluation of plant-derived foods within an immunometabolic context.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"1008-1029"},"PeriodicalIF":2.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147778465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of High-Throughput Sequencing in the Authentication and Quality Control of Herbal Material and Products.","authors":"Kwun-Tin Chan, Johnson Jor-Shing Chan, Pang-Chui Shaw","doi":"10.1055/a-2904-2063","DOIUrl":"10.1055/a-2904-2063","url":null,"abstract":"<p><strong>Abstract: </strong>Herbal material and products (HMPs) have gained worldwide popularity in the past decades. Yet the efficacy and safety of HMPs are frequently compromised by economically motivated adulteration, species substitution, and mislabelling. Conventional authentication methods, relying on morphology and chemical profiling, often fall short when dealing with complex multi-ingredient mixtures or trace contaminants. DNA-based methods have been widely adopted by various pharmacopeias, and the rapid development of high-throughput sequencing technology has shown promising identifying power in related fields.</p><p><strong>Abstract: </strong>This review examines the transformative application of high-throughput sequencing (HTS) in the authentication and quality control of herbal material and product by exploring the advanced HTS techniques, including short- and long-read amplicon metabarcoding, shotgun metabarcoding, genome skimming, superbarcoding, and target capture. These approaches offer unprecedented resolution, enabling simultaneous, multi-kingdom detection of prescribed species, adulterants, and contaminants, even in highly processed preparations. That said, HTS has challenges such as DNA degradation, extraction biases, and limitations in quantitative accuracy; integrating these genomic tools with orthogonal chemical analyses provides a rigorous, complementary framework. The transition towards multi-omics integration, portable sequencing applications, and artificial intelligence place HTS as a cornerstone for the future of authentication and quality control.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148397411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginkgo biloba Leaf Extracts in Global Dementia and Mild Cognitive Impairment Guidelines. A Review.","authors":"Katrin Pfuhlmann, Anna Katharina Koch, Jost Langhorst","doi":"10.1055/a-2895-5345","DOIUrl":"https://doi.org/10.1055/a-2895-5345","url":null,"abstract":"<p><strong>Abstract: </strong>Age-related diseases like dementia are increasing globally, with limited treatments. Extracts of <i>Ginkgo biloba</i> are often used alongside synthetic drugs. The aim of this article was to identify clinical guidelines from countries around the world to assess recommendations on the use of <i>Ginkgo biloba</i> in dementia, mild cognitive impairment, and prevention. PubMed was systematically searched for guidelines on dementia and mild cognitive impairment on the 25th of April 2023. A search update was conducted on the 3rd of March 2026. Additional guidelines were identified via citation searching and organisations. Seventy-seven guideline documents were included: 47 on dementia (subtype not further defined), 25 on Alzheimer's disease, 19 on vascular dementia, 18 on mild cognitive impairment, and seven on prevention, with some guidelines including multiple mentions. The guidelines come from four continents and 28 countries, plus seven international guidelines. Regarding <i>Ginkgo biloba</i>, 52 give positive, 18 negative, and 26 neutral recommendations (double mentions possible); 27 do not mention it. <i>Ginkgo biloba</i> is referred to in guidelines worldwide, usually without reference to specific preparations. Preparation standardisation would be beneficial.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148631172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Potential of Limonium brasiliense Extracts in Helicobacter pylori-Associated Gastric Pathologies.","authors":"Augusto Santos Borges, Ana Carolina Guidi, Mariana Nascimento de Paula, Eloisa Lorenzi da Silva, Gabriel Jorge Fagundes, Marco Aurélio Valadão Fagundes, Rodrigo Rezende Kitagawa, Rita de Cássia Ribeiro Gonçalves, Daniela Cristina de Medeiros Araújo, João Carlos Palazzo de Mello","doi":"10.1055/a-2897-9285","DOIUrl":"10.1055/a-2897-9285","url":null,"abstract":"<p><strong>Abstract: </strong><i>Helicobacter pylori</i> infection is related to several gastric pathologies, including gastritis, peptic ulcer, and gastric cancer. Substances capable of eradicating <i>H. pylori</i> have been subject to the attention of researchers. This study evaluated a fully characterized ethyl acetate fraction, obtained from the rhizomes of <i>Limonium brasiliense</i>, and its microparticulate pharmaceutical form against <i>H. pylori</i>, as well as their potential immunomodulatory and antitumor activities associated with <i>H. pylori</i> infection. Ultra-high-performance liquid chromatography analysis of ethyl acetate fraction indicated the presence of samaragenins A and B along with gallic acid, epigallocatechin, and epigallocatechin-3-<i>O</i>-gallate. Antioxidant activity showed half-maximum inhibitory concentration values of 5.7 (95% confidence interval: 5.21 - 6.83), 31.9 (95% confidence interval: 19.57 - 51.99), and 33.1 (95% confidence interval: 24.4 - 44.73) µg/mL for HOCl, O₂ ^•-, and NO, respectively. Ethyl acetate fraction exerted minimal inhibitory concentration values of 1,024 and 128 µg/mL against the <i>H. pylori</i> (ATCC 43 504, clinical isolate <i>H. pylori</i> strain). <i>In vivo</i> treatments in mice with ethyl acetate fraction and microparticulate ethyl acetate fraction (100 mg/kg, in single daily doses for 20 consecutive days) improved gastric pathology in infected animals, reducing urease positivity (14.3% vs. 54.5% in untreated controls), inflammation (tumor necrosis factor alpha, interleukin 1<i>β</i>, and interleukin 6 reduced, p < 0.05), and morphological damage compared with the untreated control group. <i>H. pylori</i> urease was inhibited with half-maximum inhibitory concentration of 521.6 µg/mL (95% confidence interval: 419.2 - 649.0). In addition, ethyl acetate fraction showed antiproliferative activity against gastric adenocarcinoma cells (half cytotoxic concentration of 21.7, 95% confidence interval: 18.1 - 26.04). In <i>Escherichia coli</i> lipopolysaccharide-stimulated macrophages, ethyl acetate fraction reduced the tumor necrosis factor alpha and interleukin 6 production by 16% and 35%, respectively. These results indicate the potential of <i>L. brasiliense</i> as a source of compounds capable of attenuating gastric diseases related to <i>H. pylori</i> infection.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148278489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}