Planta medica最新文献

{"title":"Uncovering Anti-Inflammatory Activity of Ginsenoside Rg1 in a Wound-Inured Zebrafish Model by GC-MS-based Chemical Profiling.","authors":"Su-Jung Hsu, Min He, Luis Francisco Salomé-Abarca, Young Hae Choi, Mei Wang","doi":"10.1055/a-2606-6705","DOIUrl":"https://doi.org/10.1055/a-2606-6705","url":null,"abstract":"<p><p>There is growing evidence highlighting the pivotal role of cellular metabolic adaptation in governing diverse immune responses, as well as the capacity of immune cells to alter metabolic preferences. In both scenarios, the prospect of leveraging bioactive compounds to induce metabolic reprogramming emerges as a novel adjuvant strategy for clinical immunotherapy. Rg1, a major active ginsenoside found in ginseng roots, has the potential to function as a glucocorticoid receptor agonist. Unraveling the intricate relationship between anti-inflammatory functions and the metabolic effects of ginsenosides and glucocorticoids may contribute to the identification of metabolic biomarkers associated with anti-inflammation. This research aims to determine endogenous metabolic response differences evoked by Rg1 and glucocorticoids underlying <i>in vivo</i> anti-inflammatory responses. The metabolic impact, particularly on primary metabolites, was assessed in zebrafish embryos using gas chromatography-mass spectrometry (GC-MS) in conjunction with metabolic pathways analysis via the KEGG pathway database. Our results indicated that Rg1 possesses a similar effect in alleviating inflammation in treating injured zebrafish as beclomethasone. The anti-inflammatory effects of Rg1 are achieved by inhibiting the neutrophils and macrophages toward the amputated edges and upregulating gene expression associated with pro-inflammatory cytokines. The anti-inflammatory effects of Rg1 also include changes in fatty-acid metabolism and downstream aromatic amino acids in the TCA cycle. Therefore, Rg1 may be a promising drug candidate for treating inflammatory responses and a valuable supplement for enhancing immune regulation.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico and in vitro screening of medicinal plants from Brazilian traditional medicine for anti-Helicobacter pylori activity.","authors":"Campili Mendes, Jéssica Raquel Borges Monteiro, Rodrigo de Almeida Romagna, Aliny Rodrigues de Jesus da Conceição, Pedro Henrique Tregnano, Amanda Eiriz Feu, Rita de Cássia Ribeiro Gonçalves, Warley de Souza Borges, Ricardo Machado Kuster, Rodrigo Rezende Kitagawa","doi":"10.1055/a-2635-4268","DOIUrl":"https://doi.org/10.1055/a-2635-4268","url":null,"abstract":"<p><p>Medicinal plants and their phytocompounds are valuable shortcuts for discovering new, safer biologically active compounds or herbal medicines with reduced adverse effects. In this study, medicinal plant species were initially selected from Brazilian traditional medicine using database from <i>in silico</i> and <i>in vitro</i> studies. Virtual screening study was carried out with a phytochemical database previously reported in the literature. The biological activity was evaluated <i>in silico</i> by PASS Online and molecular docking, then validated by <i>in vitro</i> anti-<i>Helicobacter pylori</i> assay. The chemical profile of the species was obtained by ESI(±)FT-ICR MS and LC-MS-DAD analysis. <i>Vernonia condensata</i>, <i>Bauhinia forficata</i>, <i>Jatropha gossypiifolia</i>, and <i>Sonalum paniculatum</i> were selected based on a survey of the literature for use of gastric diseases and anti-<i>Helicobacter pylori</i> potential. After PASS analysis, J<i>atropha gossypiifolia</i> was selected for <i>in vitro</i> study because its compounds showed anti-<i>H. pylori</i> activity potential, inhibiting fumarate reductase enzyme. <i>J. gossypiifolia</i> extracted showed MIC of 64 µg/mL and MBC of 128 µg/mL in the <i>in vitro</i> anti-<i>H. pylori</i> assay. ESI(±)FT-ICR MS and LC-MS-DAD analysis showed compounds as luteolin (1: ), isovitexin (2: ), luteolin-7-O-glucoside (3: ), isoorientin (4: ), and 3-genistein-8-C-glucoside (5: ). Molecular docking analysis showed a potential interaction of compounds in the enzyme active site such as hydrogen bonds with Arg404 and a similar interaction to fumaric acid, except for isoorientin (3: ). <i>J. gossypiifolia</i> showed promising activity and may represent a future alternative to treat <i>H. pylori</i> infections and their deleterious effects, reinforcing the therapeutic potential of this plant.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144294784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Medicinal Chemistry of Cyanidin and its Glycoside Derivatives: Focus on the Antiproliferative and Potential Anticancer Activity.","authors":"Abdur Rauf, Ahmed Olatunde, Rahaf Ajaj, Nabia Hafeez, Mohamed Y Zaky, Rezaul Islam, Md Mozahidul Islam, Giovanni Ribaudo","doi":"10.1055/a-2634-4300","DOIUrl":"https://doi.org/10.1055/a-2634-4300","url":null,"abstract":"<p><p>Cyanidin and its glucosides are anthocyanins belonging to the class of flavonoid phytochemicals. These pigments give fruits and vegetables their typical reddish-purple nuance, and their peculiar chemical features result in a remarkable ability to neutralize reactive oxygen species and other mutagens. Thus, both cyanidin and its glycosides were proposed as candidates for chemoprevention, as anticancer agents and as adjuvant therapies. Indeed, the compounds were investigated through various in vitro and in vivo models of colon, breast, kidney, prostate and liver cancer and glioma. Cyanidin and its derivatives have been found to inhibit key signaling pathways, such as PI3K/Akt, MAPK, and NF-κB, which can reduce cancer cell growth, induce apoptosis, and suppress metastasis. In the first part of the review, the chemical properties of cyanidin and its glycoside analogues will be discussed. Then, an overview of in vitro evidence on activity will be presented, followed by a report on preclinical and clinical data together with comments on the involved mechanisms. Eventually, the aspect of pharmacokinetic properties, bioavailability and formulation will be dissected. Overall, the review indicates that cyanidin and its derivatives could be effective anticancer agents but also calls for a deeper understanding of the molecular mechanisms underlying their bioactivity. Despite promising results, resolving issues like stability, absorption, and targeted distribution is crucial to maximize their therapeutic potential. More research is needed to develop innovative cyanidin-based formulations for efficient cancer treatment.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144286116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Separation and Cytotoxicity of Enzymatic Transformed Prosaikogenins from Bupleurum falcatum.","authors":"Hyejin Ko, Kyoung Jin Lee, Kwangho Song, In Jin Ha, Yeong Shik Kim","doi":"10.1055/a-2592-0968","DOIUrl":"https://doi.org/10.1055/a-2592-0968","url":null,"abstract":"<p><p>Saikosaponins, bioactive compounds derived from <i>Bupleurum falcatum</i> roots, have limited applications due to their low bioavailability and the absence of efficient large-scale separation methods. To address this, an enzymatic transformation <i>in vitro</i> with cellulase was employed to remove glucose at the C-3 position, producing lipophilic prosaikogenins. These metabolites were separated using countercurrent chromatography (CCC) and preparative HPLC. The optimal CCC solvent system was determined to be dichloromethane/methanol/water (4 : 3 : 2, v/v/v). Prosaikogenin F and prosaikogenin G (PSG G) were isolated from the deglycosylated fraction, and the effect of rotation speed on compound retention was examined. Further enzymatic biotransformation using <i>α</i>-L-rhamnosidase and cellulase resulted in the isolation of prosaikogenins E₁ and E₃. The efficient separation of these four prosaikogenins was achieved through a combination of enzymatic transformation and CCC. Of these, PSG G demonstrated the strongest anticancer activity against the cancer cell lines MDA-MB-468, HepG2, and HCT116, while exhibiting lower toxicity in normal cells, supporting its potential as an effective anticancer agent. This study presents a highly efficient enzymatic transformation and separation strategy that can aid in the pharmaceutical development of saikosaponin derivatives.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144266953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compositional Analysis of Glycyrrhiza uralensis, G. inflata, and G. glabra after Honey Processing, and the Cardioprotective Effects in Zebrafish Embryos.","authors":"Jiayi Wang, Wenxin Wang, Binghan Liu, Xiaoyu Fan, Shucen Liu, Guangchao Yang, Jining Liu, Tulin Lu, Lihong Chen","doi":"10.1055/a-2615-7291","DOIUrl":"https://doi.org/10.1055/a-2615-7291","url":null,"abstract":"<p><p>The \"<i>Shang Han Lun</i>\" indicates that honey-processed licorice protects the heart better than raw licorice. Ten major constituents in honey-processed licorice samples were quantified. Protective effects of honey-processed licorices against doxorubicin-induced cardiotoxicity were assessed in zebrafish larvae. Network pharmacology analysis based on the ten target constituents was conducted. Results showed glabridin was lowest in honey-processed <i>Gg</i>, while total content of six components (such as liquiritin) was highest in honey-processed <i>Gu</i>, followed by honey-processed <i>Gi</i>, and lowest in honey-processed <i>Gg</i>. Pharmacological results indicated that honey-processed <i>Gu</i> and <i>Gi</i> significantly improved doxorubicin-induced abnormal pericardial edema and increased venous sinus-arterial bulb distance in larvae. The pericardial area was reduced by 23% and 20%, respectively compared to the model group, and the distances reduced to 81% and 83.3% of the model group, respectively. Although improvements in pericardial edema were rare in the <i>honey-processed Gg</i> group, it reversed venous sinus-arterial bulb distance increase. These results indicate that honey-processed <i>Gu</i> and honey-processed <i>Gi</i> can significantly protect zebrafish embryos against the effects of doxorubicin-induced cardiotoxicity, namely, abnormal heart rate, pericardial edema, and elongation of the venous sinus-arterial bulb distance, whereas honey-processed <i>Gg</i> can only significantly reverse the doxorubicin-induced increase in the venous sinus-arterial bulb distance. Network pharmacology analysis predicted that these constituents have potential for the treatment of metabolic abnormalities and cellular senescence related diseases caused by reactive oxygen species induction, linking to Rap1 pathways. Honey-processed <i>Gu</i> and honey-processed <i>Gi</i> had stronger cardioprotective effects on zebrafish embryos than honey-processed <i>Gg</i> possibly because of differences in composition.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144266951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: A Neutral Glucan Extracted from Dried Ginger (Zingiberis Rhizoma): Preparation, Structure Characterization, and Immunomodulatory Activity.","authors":"Long Sun, Xing Ni, Yulin Liu, Yantao Jiang, Pei-Pei Wang, Jingdong Gao","doi":"10.1055/a-2627-5735","DOIUrl":"10.1055/a-2627-5735","url":null,"abstract":"","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144266952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a carrot cell plant growth regulators-free system to produce a low-cost vac-cine candidate.","authors":"Christian Carreño-Campos, Sahara D Elguea Zarate, Andrea Romero-Maldonado, Maria Luisa Villarreal, Sergio Rosales-Mendoza, Anabel Ortiz Caltempa","doi":"10.1055/a-2626-4703","DOIUrl":"https://doi.org/10.1055/a-2626-4703","url":null,"abstract":"<p><p>Parkinson´s disease is characterized by an abnormal accumulation of alpha synuclein (α-syn) in dif-ferent regions of the central nervous system. At present, pharmacological treatment is effective, but it is a palliative condition. Immunotherapy is considered an alternative to treat Parkinson´s disease, and plants are a convenient alternative platform for biopharmaceutical production. When compared to other systems, plants are particularly attractive because they offer cost-effectiveness, large-scale production, and enhanced safety. Therefore, this study aimed to establish a carrot cell suspension culture for the production of cLTB-Syn, a vaccine candidate against Parkinson´s disease. The con-venience of MS medium optimization was demonstrated. Transgenic callus cultures were maintained and adapted on solid MSU9 medium without phytohormones, followed by growth kinetics in suspen-sion cultures. The maximum biomass yield was 15.8 ± 0.35 g/L DW at 14 days of culture, with a growth rate of µ= 0.1034/d and td=6.7 days. The cLTB-Syn protein production reached a maximum value of 2.62 ± 0.03 µg/g DW, representing a 1.6-fold increase over the initial culture time. Finally, the presence of the transgene was confirmed by PCR, and the integrity of cLTB-Syn protein was deter-mined by dot blot assays. This study presents evidence of a promising system for a toxin free bio-pharmaceutical production, which has the potential to be scaled up for large manufacturing, at a low cost.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antidiabetic Potential of Sophora Species: Mechanisms, Bioactive Constituents, and Therapeutic Prospects.","authors":"Mahdis Mousavi, Mahdi Moridi Farimani, Khosrow Kashfi, Asghar Ghasemi","doi":"10.1055/a-2597-8133","DOIUrl":"10.1055/a-2597-8133","url":null,"abstract":"<p><p>Diabetes is a major global health concern, and achieving optimal glycemic control remains a challenge for many patients. Despite the availability of current antidiabetic medications, about two-thirds of patients worldwide fail to achieve adequate glycemic control, underscoring the need for novel treatments. Herbal medicine has significantly contributed to drug discovery, and <i>Sophora</i>, a genus in the Fabaceae family, has long been used in traditional medicine. Preclinical studies suggest that various chemical constituents of <i>Sophora</i> exhibit antidiabetic properties. This review summarizes <i>in vitro</i> and <i>in vivo</i> evidence on the antidiabetic effects of <i>Sophora,</i> highlighting its active ingredients and mechanisms of action. A literature search was conducted using Web of Science, Scopus, PubMed, and Google Scholar with the keywords '<i>Sophora</i>', 'diabetes', and 'herbal medicine'. Studies indicate that <i>Sophora</i> reduces fasting glucose in type 1 and type 2 diabetes (T2D) by approximately 33% and 37%, respectively. Additionally, it decreases body weight, improves glucose tolerance, reduces insulin resistance, and enhances lipid profiles in T2D. The antidiabetic mechanisms of <i>Sophora</i> involve the activation of phospholipase C-protein kinase C (PLC-PKC), phosphatidylinositol-3-kinase (PI3K)-Akt (PI3K-Akt), and adenosine monophosphate (AMP)-activated protein kinase (AMPK) pathways, leading to enhanced glucose uptake in the skeletal muscle. Furthermore, <i>Sophora</i> activates the PI3K-Akt pathway and inhibits nuclear factor-kappa B (NF<i>κ</i>B), thereby reducing hepatic gluconeogenesis and inflammation. Among its active constituents, flavonoids exhibit the most significant antidiabetic activity. While <i>Sophora</i> holds promise for antidiabetic drug development, further preclinical studies assessing sex differences and long-term safety are required before progressing to human clinical trials.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144042437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polycyclic Polyprenylated Acylphloroglucinols from Hypericum himalaicum.","authors":"Guang-Hui Liu, Fan Wu, Xue-Yan Huo, Hong-Bing Sun, Zhuo-Lin Jin, Yu-Cheng Gu, Da-Le Guo, Yan Zhou","doi":"10.1055/a-2596-3029","DOIUrl":"10.1055/a-2596-3029","url":null,"abstract":"<p><p>Six previously undescribed polycyclic polyprenylated acylphloroglucinols (PPAPs) with a vicinal diol moiety (1: -6: ) were isolated from the whole plant of <i>Hypericum himalaicum</i>. Their structures were established through a comprehensive analysis of HRMS and 1D and 2D NMR data, while the absolute configurations were determined using the Mo₂(OAc)₄-induced circular dichroism (ICD), ECD, and NMR calculations. Compound 1: attenuated the secretion of NO, TNF-<i>α</i>, and IL-6, downregulated the protein expression of COX-2 and iNOS, and inhibited the release of ROS in LPS-induced RAW264.7 macrophages. Further investigation revealed that the anti-inflammatory effects may be attributed to the inhibition of the NF-<i>κ</i>B and NLRP3 signaling pathways.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Hepatoprotective Effects of Naringin: A Systematic Review and Meta-Analysis of Preclinical Evidence.","authors":"Muhammed Fayaz, Gollapalle Lakshminarayanashastry Viswanatha, Hanumanthappa Shylaja, Krishnadas Nandakumar","doi":"10.1055/a-2595-7650","DOIUrl":"https://doi.org/10.1055/a-2595-7650","url":null,"abstract":"<p><p>This study aimed to perform a systematic review and meta-analysis on the hepatoprotective effects of naringin based on the pre-clinical evidence.A detailed literature search was performed using online databases such as Google Scholar, PubMed, Scopus, and EMBASE. Based on the predefined inclusion and exclusion criteria, 20 studies were considered for meta-analysis.The outcomes of the meta-analysis revealed that naringin improved liver function by reducing the elevated levels of ALT, AST, GGT, LDH, ALP, and bilirubin. It improved the enzymatic and non-enzymatic antioxidants, such as SOD, catalase, GSH, GST, GR, and GPx (p < 0.05 for all the parameters), while reducing the LPO/MDA levels (p < 0.05). NAR treatment also alleviated the levels of inflammatory mediators (IL-1<i>β</i>, IL-6, and TNF-<i>α</i>, p < 0.001 for all the parameters; NF-<i>κ</i>B, p = 0.29) in various animal models of liver injury. In addition, NAR significantly reduced the caspase-3 and Bax/Bcl-2 ratio (p < 0.05) compared to the control group. Furthermore, naringin treatment has normalised the liver and body weights compared to the disease control group.This systematic review and meta-analysis demonstrate that naringin significantly improved the liver function in various animal models of liver injury, via potent antioxidant and anti-inflammatory mechanisms.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}