Planta medica最新文献_第2页

Farnesiferol B and Kamolonol Isolated from Ferula assa-foetida are Potent BACE1 Inhibitors with Neuroprotective Effects. 从阿魏中分离的法尼铁醇B和卡莫洛酚是有效的BACE1抑制剂，具有神经保护作用。

IF 2 4区医学

Planta medica Pub Date : 2025-09-11 DOI: 10.1055/a-2689-8035

Jong Min Oh, Woong-Hee Shin, Bomi Kim, Eonmi Kim, Hyun Ju Son, Hoon Kim

{"title":"Farnesiferol B and Kamolonol Isolated from Ferula assa-foetida are Potent BACE1 Inhibitors with Neuroprotective Effects.","authors":"Jong Min Oh, Woong-Hee Shin, Bomi Kim, Eonmi Kim, Hyun Ju Son, Hoon Kim","doi":"10.1055/a-2689-8035","DOIUrl":"10.1055/a-2689-8035","url":null,"abstract":"Five compounds were isolated from Ferula assa-foetida and their beta-secretase 1 inhibitory activities were evaluated. Farnesiferol B and kamolonol showed potent beta-secretase 1 inhibitory activity with IC50 values of 8.11 and 1.00 µM and competitive inhibition patterns against beta-secretase 1 with Ki values of 6.51 and 0.41 µM, respectively. In silico pharmacokinetics showed that farnesiferol B was predicted to have high gastrointestinal absorption and blood-brain barrier permeability. In cell studies, farnesiferol B and kamolonol were nontoxic to normal Madin-Darby canine kidney and neuroblastoma cells, and both showed protective effects on neuroblastoma cells for Aβ42-induced neurotoxicity. In molecular docking simulations, we found that the efficacy of the compounds may be related to their interaction with the flap region and hydrogen bonding with ARG368. In addition, molecular dynamics simulation of kamolonol showed the ligand maintained its stability in interaction with the loop residues. These results show that farnesiferol B and kamolonol are potent beta-secretase 1 inhibitors with neuroprotective effects, suggesting that they are potential candidates for the treatment of neurodegenerative disorders, such as Alzheimer's disease.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative Analysis and Simultaneous Characterization of Triterpenoids and Phenolics in Inonotus obliquus (Chaga) Using LC-PDA-ELSD and LC-DAD-QToF. 用LC-PDA-ELSD和LC-DAD-QToF定量分析和同时表征桦茸中三萜和酚类物质。

IF 2 4区医学

Planta medica Pub Date : 2025-09-11 DOI: 10.1055/a-2689-8131

Bharathi Avula, Kumar Katragunta, Kiran Kumar Tatapudi, Ikhlas A Khan

{"title":"Quantitative Analysis and Simultaneous Characterization of Triterpenoids and Phenolics in Inonotus obliquus (Chaga) Using LC-PDA-ELSD and LC-DAD-QToF.","authors":"Bharathi Avula, Kumar Katragunta, Kiran Kumar Tatapudi, Ikhlas A Khan","doi":"10.1055/a-2689-8131","DOIUrl":"10.1055/a-2689-8131","url":null,"abstract":"Inonotus obliquus is widely recognized as the Chaga mushroom. Chaga contains various bioactive compounds, including polysaccharides, triterpenoids, polyphenols, and melanin. To address the characterization and quantitative analysis of triterpenoids and phenolics in Chaga, a multi-analytical approach has been developed combining LC-PDA-ELSD and LC-DAD-QToF. These methods were designed to quantify 11 compounds, comprising seven triterpenoids and four fatty acids, using LC-PDA-ELSD, and four phenolics using the LC-DAD-QToF method. Calibration curves for these compounds demonstrated excellent linearity within the tested range. The methods exhibited high precision, with intra- and inter-day relative standard deviations below 3% and recoveries ranged from 91% to 104%. The validated methods were applied to analyze eleven sclerotia samples, one mycelium sample, three grain-based samples, and eighteen dietary supplements. Results revealed that eight of the eighteen supplements (44%) contained ground mycelium, which primarily showed the presence of fatty acids but lacks detectable levels of triterpenoid and phenolic markers characteristic of Chaga. Triterpenoids and hispidin, identified as key bioactive compounds, were detected in eight (44%) of the eighteen supplements; however, these products also contained fatty acids and/or betulin. Two (11%) of the 18 supplements showed the presence of phenolic compounds only; no triterpenoids were detected. Additionally, untargeted metabolomic screening using LC-DAD-QToF tentatively identified 103 compounds from diverse chemical groups, including nine reference compounds. These findings provide valuable insights for the quality assessment of dietary or food supplements marketed as containing Chaga.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factors Influencing Clinical Trials of Herbal Medicinal Products - Using Ginger as Example. 影响中药临床试验的因素——以生姜为例

IF 2 4区医学

Planta medica Pub Date : 2025-09-08 DOI: 10.1055/a-2686-6315

Ingrid Hook, Liselotte Krenn, Barbara Steinhoff, Evelyn Wolfram

引用次数: 0

Cupressuflavone Isolated from Cupressus torulosa Ameliorates Diabetic Nephropathy by Inhibiting Oxidative Stress and Inflammation Through Nrf-2/NF-κB Signalling Axis. 从托鲁沙柏树中提取的柏素黄酮通过Nrf-2/ NF-κB信号轴抑制氧化应激和炎症，改善糖尿病肾病。

IF 2 4区医学

Planta medica Pub Date : 2025-09-04 DOI: 10.1055/a-2686-3928

Kishan Singh, Karan Singh Yadav, Arti Shukla, Vipin Kumar Singh, Narayan Prasad Yadav, Madhav Nilakanth Mugale, Kapil Dev

{"title":"Cupressuflavone Isolated from Cupressus torulosa Ameliorates Diabetic Nephropathy by Inhibiting Oxidative Stress and Inflammation Through Nrf-2/NF-κB Signalling Axis.","authors":"Kishan Singh, Karan Singh Yadav, Arti Shukla, Vipin Kumar Singh, Narayan Prasad Yadav, Madhav Nilakanth Mugale, Kapil Dev","doi":"10.1055/a-2686-3928","DOIUrl":"10.1055/a-2686-3928","url":null,"abstract":"Cupressuflavone (CTM-01), a potential biflavonoid isolated from Cupressus torulosa exhibited antimicrobial, analgesic, cytotoxic and wound-healing properties. The present study aims to evaluate the renoprotective effects through in vitro and in vivo models as well as elucidate the underlying mechanisms of its nephroprotective action under diabetic conditions. The in vitro effects of CTM-01 on cell viability (25, 50, and 100 µM) and intracellular ROS production were evaluated in cultured normal rat proximal epithelial cells (NRK-52E) grown under high glucose conditions (30 mM) while streptozotocin (STZ) induced rats were treated with CTM-01 at 25 mg/kg for four weeks and the effects on different biochemical, histological, and molecular parameters were studied. The oral administration of CTM-01 (25 mg/kg) in diabetic rats restored the fasting blood glucose to normal control levels and markedly ameliorated renal dysfunction as evidenced by a significant decrease in serum creatinine, urea, and albumin. Additionally, CTM-01-treated rats exhibited a significant increase in the levels of renal antioxidants such as SOD, CAT, and GSH as well as decreased MDA content against diabetic control rats and restored kidney damage in CTM-01-treated diabetic rats. Moreover, CTM-01 significantly increased the level of Nrf-2 and downregulated the expression of p-NF-κB. This novel study provides strong evidence to support the potent anti-oxidative and renoprotective properties of CTM-01 in alleviating oxidative damage and inflammation through the suppression of the Nrf-2/NF-κB signalling axis for the effective management of diabetic nephropathy.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144883465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vivo, Ex Vivo, and In Vitro Antihyperglycemic Evaluation of Croton ehrenbergii. 巴豆在体内、离体和体外的抗高血糖作用评价。

IF 2 4区医学

Planta medica Pub Date : 2025-09-04 DOI: 10.1055/a-2689-5059

Mónica Aideé Díaz-Román, Maria Yolanda Rios, Juan-José Acevedo-Fernández, A Berenice Aguilar-Guadarrama

{"title":"In Vivo, Ex Vivo, and In Vitro Antihyperglycemic Evaluation of Croton ehrenbergii.","authors":"Mónica Aideé Díaz-Román, Maria Yolanda Rios, Juan-José Acevedo-Fernández, A Berenice Aguilar-Guadarrama","doi":"10.1055/a-2689-5059","DOIUrl":"https://doi.org/10.1055/a-2689-5059","url":null,"abstract":"Various Croton species have been traditionally used to treat diabetes; however, the antidiabetic potential and safety of many of these species remain poorly understood. This study evaluated the chemical composition, antihyperglycemic activity, insulin-sensitizing effect, and acute oral toxicity of C. ehrenbergii. Dichloromethane, ethyl acetate, n-butanol, and aqueous residue fractions were obtained via liquid-liquid extraction from the hydroalcoholic extract obtained via maceration of the aerial parts. The primary compounds isolated from the fractions using column chromatography and identified by 1D nuclear magnetic resonance spectroscopy were 7,4'-di-O-methylnaringenin, β-sitosterol, tiliroside, rutin, nicotiflorin, isoquercetin, and l-quebrachitol. The in vivo antihyperglycemic activity of these compounds was assessed using oral sucrose and glucose tolerance tests, and the most active fractions were evaluated ex vivo to explore the mechanisms of action. The extract, fractions, and compounds were tested in vitro for their ability to inhibit α-glucosidase and protein tyrosine phosphatase 1B (PTP1B) as well as for their agonistic activity on PPAR-γ. Tiliroside and nicotiflorin moderately inhibited PTP1B and α-glucosidase; whereas, l-quebrachitol acted as a PPAR-γ agonist. Acute oral toxicity studies indicated that the extract was safe at the tested dose. These results provide the first scientific evidence of the antihyperglycemic properties and preliminary safety of C. ehrenbergii.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Therapeutic Potential of Topically Applied Essential Oils in Preventing or Treating Early Borrelia burgdorferi Infection: A Review. 局部应用精油预防或治疗早期伯氏疏螺旋体感染的治疗潜力：综述。

IF 2 4区医学

Planta medica Pub Date : 2025-09-04 DOI: 10.1055/a-2640-4274

Keren Dolan, John Courie, Michael Tims

{"title":"The Therapeutic Potential of Topically Applied Essential Oils in Preventing or Treating Early Borrelia burgdorferi Infection: A Review.","authors":"Keren Dolan, John Courie, Michael Tims","doi":"10.1055/a-2640-4274","DOIUrl":"10.1055/a-2640-4274","url":null,"abstract":"Lyme disease, caused by Borrelia burgdorferi, presents significant diagnostic challenges, often leading to delayed treatment and decreased therapeutic response to conventional antibiotics. This review aims to evaluate the potential of plant essential oils, known for their bacteriostatic, bactericidal, and anti-quorum sensing properties, as prophylactic, adjunct, or complementary treatments during the early stages of B. burgdorferi infection. The authors explore how these essential oils can target adaptive mechanisms and interactions of B. burgdorferi, including complement regulator-acquiring surface proteins (CRASPs), immune modulation, motility, chemotaxis, biofilm formation, efflux-pump mechanisms, and cyst formation. The authors identify current research gaps and propose frameworks to substantiate the clinical efficacy of essential oils for Lyme disease treatment. This review indicates that essential oils have multifaceted therapeutic potential and could provide a viable option for early intervention in Lyme disease. Further research is necessary to confirm their clinical applicability and safety.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polycyclic Polyprenylated Acylphloroglucinols from Hypericum himalaicum. 喜马拉雅金丝桃多环聚丙烯化酰基间苯三酚。

IF 2 4区医学

Planta medica Pub Date : 2025-09-01 Epub Date: 2025-04-28 DOI: 10.1055/a-2596-3029

Guang-Hui Liu, Fan Wu, Xue-Yan Huo, Hong-Bing Sun, Zhuo-Lin Jin, Yu-Cheng Gu, Da-Le Guo, Yan Zhou

引用次数: 0

Separation and Cytotoxicity of Enzymatic Transformed Prosaikogenins from Bupleurum falcatum. 柴胡酶转化原柴胡苷元的分离及细胞毒性研究。

IF 2 4区医学

Planta medica Pub Date : 2025-09-01 Epub Date: 2025-06-10 DOI: 10.1055/a-2592-0968

Hyejin Ko, Kyoung Jin Lee, Kwangho Song, In Jin Ha, Yeong Shik Kim

{"title":"Separation and Cytotoxicity of Enzymatic Transformed Prosaikogenins from Bupleurum falcatum.","authors":"Hyejin Ko, Kyoung Jin Lee, Kwangho Song, In Jin Ha, Yeong Shik Kim","doi":"10.1055/a-2592-0968","DOIUrl":"10.1055/a-2592-0968","url":null,"abstract":"Saikosaponins, bioactive compounds derived from Bupleurum falcatum roots, have limited applications due to their low bioavailability and the absence of efficient large-scale separation methods. To address this, an enzymatic transformation in vitro with cellulase was employed to remove glucose at the C-3 position, producing lipophilic prosaikogenins. These metabolites were separated using countercurrent chromatography (CCC) and preparative HPLC. The optimal CCC solvent system was determined to be dichloromethane/methanol/water (4 : 3 : 2, v/v/v). Prosaikogenin F and prosaikogenin G (PSG G) were isolated from the deglycosylated fraction, and the effect of rotation speed on compound retention was examined. Further enzymatic biotransformation using α-L-rhamnosidase and cellulase resulted in the isolation of prosaikogenins E1 and E3. The efficient separation of these four prosaikogenins was achieved through a combination of enzymatic transformation and CCC. Of these, PSG G demonstrated the strongest anticancer activity against the cancer cell lines MDA-MB-468, HepG2, and HCT116, while exhibiting lower toxicity in normal cells, supporting its potential as an effective anticancer agent. This study presents a highly efficient enzymatic transformation and separation strategy that can aid in the pharmaceutical development of saikosaponin derivatives.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"590-598"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144266953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uncovering Anti-Inflammatory Activity of Ginsenoside Rg1 in a Wound-Inured Zebrafish Model by GC-MS-based Chemical Profiling. 基于gc - ms的化学分析揭示了人参皂苷Rg1在斑马鱼创伤模型中的抗炎活性。

IF 2 4区医学

Planta medica Pub Date : 2025-09-01 Epub Date: 2025-06-16 DOI: 10.1055/a-2606-6705

Su-Jung Hsu, Min He, Luis Francisco Salomé-Abarca, Young Hae Choi, Mei Wang

{"title":"Uncovering Anti-Inflammatory Activity of Ginsenoside Rg1 in a Wound-Inured Zebrafish Model by GC-MS-based Chemical Profiling.","authors":"Su-Jung Hsu, Min He, Luis Francisco Salomé-Abarca, Young Hae Choi, Mei Wang","doi":"10.1055/a-2606-6705","DOIUrl":"10.1055/a-2606-6705","url":null,"abstract":"There is growing evidence highlighting the pivotal role of cellular metabolic adaptation in governing diverse immune responses, as well as the capacity of immune cells to alter metabolic preferences. In both scenarios, the prospect of leveraging bioactive compounds to induce metabolic reprogramming emerges as a novel adjuvant strategy for clinical immunotherapy. Rg1, a major active ginsenoside found in ginseng roots, has the potential to function as a glucocorticoid receptor agonist. Unraveling the intricate relationship between anti-inflammatory functions and the metabolic effects of ginsenosides and glucocorticoids may contribute to the identification of metabolic biomarkers associated with anti-inflammation. This research aims to determine endogenous metabolic response differences evoked by Rg1 and glucocorticoids underlying in vivo anti-inflammatory responses. The metabolic impact, particularly on primary metabolites, was assessed in zebrafish embryos using gas chromatography-mass spectrometry (GC-MS) in conjunction with metabolic pathways analysis via the KEGG pathway database. Our results indicated that Rg1 possesses a similar effect in alleviating inflammation in treating injured zebrafish as beclomethasone. The anti-inflammatory effects of Rg1 are achieved by inhibiting the neutrophils and macrophages toward the amputated edges and upregulating gene expression associated with pro-inflammatory cytokines. The anti-inflammatory effects of Rg1 also include changes in fatty-acid metabolism and downstream aromatic amino acids in the TCA cycle. Therefore, Rg1 may be a promising drug candidate for treating inflammatory responses and a valuable supplement for enhancing immune regulation.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"609-620"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stachybotrins G and H, Two New Phenylspirodrimane Derivatives from the Fungus Stachybotrys chartarum. Stachybotrins G和H，两个新的从Stachybotrys chartarum中提取的苯螺旋体衍生物。

IF 2 4区医学

Planta medica Pub Date : 2025-09-01 Epub Date: 2025-04-22 DOI: 10.1055/a-2592-1627

Yi Wang, Kang Chen, Qiao Xing, Tao Zhang, Yuquan Xu

引用次数: 0