Planta medica最新文献

{"title":"Farnesiferol B and Kamolonol Isolated from Ferula assa-foetida are Potent BACE1 Inhibitors with Neuroprotective Effects.","authors":"Jong Min Oh, Woong-Hee Shin, Bomi Kim, Eonmi Kim, Hyun Ju Son, Hoon Kim","doi":"10.1055/a-2689-8035","DOIUrl":"10.1055/a-2689-8035","url":null,"abstract":"<p><p>Five compounds were isolated from <i>Ferula assa-foetida</i> and their beta-secretase 1 inhibitory activities were evaluated. Farnesiferol B and kamolonol showed potent beta-secretase 1 inhibitory activity with IC₅₀ values of 8.11 and 1.00 µM and competitive inhibition patterns against beta-secretase 1 with K_i values of 6.51 and 0.41 µM, respectively. <i>In silico</i> pharmacokinetics showed that farnesiferol B was predicted to have high gastrointestinal absorption and blood-brain barrier permeability. In cell studies, farnesiferol B and kamolonol were nontoxic to normal Madin-Darby canine kidney and neuroblastoma cells, and both showed protective effects on neuroblastoma cells for Aβ₄₂-induced neurotoxicity. In molecular docking simulations, we found that the efficacy of the compounds may be related to their interaction with the flap region and hydrogen bonding with ARG368. In addition, molecular dynamics simulation of kamolonol showed the ligand maintained its stability in interaction with the loop residues. These results show that farnesiferol B and kamolonol are potent beta-secretase 1 inhibitors with neuroprotective effects, suggesting that they are potential candidates for the treatment of neurodegenerative disorders, such as Alzheimer's disease.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"933-942"},"PeriodicalIF":2.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vivo, Ex Vivo, and In Vitro Antihyperglycemic Evaluation of Croton ehrenbergii.","authors":"Mónica Aideé Díaz-Román, Maria Yolanda Rios, Juan-José Acevedo-Fernández, A Berenice Aguilar-Guadarrama","doi":"10.1055/a-2689-5059","DOIUrl":"10.1055/a-2689-5059","url":null,"abstract":"<p><p>Various <i>Croton</i> species have been traditionally used to treat diabetes; however, the antidiabetic potential and safety of many of these species remain poorly understood. This study evaluated the chemical composition, antihyperglycemic activity, insulin-sensitizing effect, and acute oral toxicity of <i>C. ehrenbergii</i>. Dichloromethane, ethyl acetate, <i>n</i>-butanol, and aqueous residue fractions were obtained via liquid-liquid extraction from the hydroalcoholic extract obtained via maceration of the aerial parts. The primary compounds isolated from the fractions using column chromatography and identified by 1D nuclear magnetic resonance spectroscopy were 7,4'-di-<i>O</i>-methylnaringenin, <i>β</i>-sitosterol, tiliroside, rutin, nicotiflorin, isoquercetin, and l-quebrachitol. The <i>in vivo</i> antihyperglycemic activity of these compounds was assessed using oral sucrose and glucose tolerance tests, and the most active fractions were evaluated <i>ex vivo</i> to explore the mechanisms of action. The extract, fractions, and compounds were tested <i>in vitro</i> for their ability to inhibit <i>α</i>-glucosidase and protein tyrosine phosphatase 1B (PTP1B) as well as for their agonistic activity on PPAR-<i>γ</i>. Tiliroside and nicotiflorin moderately inhibited PTP1B and <i>α</i>-glucosidase; whereas, l-quebrachitol acted as a PPAR-<i>γ</i> agonist. Acute oral toxicity studies indicated that the extract was safe at the tested dose. These results provide the first scientific evidence of the antihyperglycemic properties and preliminary safety of <i>C. ehrenbergii</i>.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"923-932"},"PeriodicalIF":2.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Olive Polyphenol Hydroxytyrosol Enhances Autophagy and Heme Oxygenase-1 Expression in Aortic Endothelial Cells and Reduces Arterial Stiffness ex vivo.","authors":"Stef Lauwers, Melissa Van Praet, Bieke Steenput, Anne-Sophie Weyns, Cédric H G Neutel, Emmy Tuenter, Nina Hermans, Lynn Roth","doi":"10.1055/a-2662-9672","DOIUrl":"10.1055/a-2662-9672","url":null,"abstract":"<p><p>Age-related arterial stiffening is a hallmark of vascular ageing and a key driver of cardiovascular disease. Oxidative stress, impaired autophagy, and extracellular matrix remodelling play an important role in the progression of aortic stiffening. Hydroxytyrosol (HT), a phenolic compound in olives, has demonstrated antioxidant properties and the ability to modulate autophagy, positioning it as a potential therapeutic for vascular ageing. In this study, we investigated the effects of HT on autophagy flux and antioxidant protein expression in human aortic endothelial cells (HAoECs). In parallel, we examined the impact of HT on arterial stiffness <i>ex vivo</i> using isolated aortic segments from wild-type (WT) and <i>Fbn1C1039G+/-</i> mice, a model of elastin fragmentation.HT treatment (50 and 100 µM; 18 h) enhanced autophagy flux in HAoECs, evidenced by increased LC3-II and p62 turnover, and reduced mTOR activity. Additionally, HT upregulated heme oxygenase-1 (HO-1), a key antioxidant enzyme. <i>Ex vivo</i> treatment of aortic segments from WT and <i>Fbn1C1039G+/-</i> mice with HT (50 µM; 18 h) restored IP₃-mediated contractions and reduced aortic stiffness in <i>Fbn1C1039G+/-</i> aortas, as demonstrated by a decreased Peterson's modulus. Although HT did not significantly affect collagen or elastin content or elastic fibre breaks in the aortic wall, it notably increased HO-1 protein levels in <i>Fbn1C1039G+/-</i> aortas.These findings demonstrate the potential of HT to mitigate oxidative stress, enhance autophagy, and reduce arterial stiffness, making it a promising nutraceutical for addressing age-related vascular dysfunction. Further long-term studies are needed to elucidate the molecular mechanisms and evaluate its sustained benefits <i>in vivo</i>.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"899-912"},"PeriodicalIF":2.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Therapeutic Potential of Topically Applied Essential Oils in Preventing or Treating Early Borrelia burgdorferi Infection: A Review.","authors":"Keren Dolan, John Courie, Michael Tims","doi":"10.1055/a-2640-4274","DOIUrl":"10.1055/a-2640-4274","url":null,"abstract":"<p><p>Lyme disease, caused by <i>Borrelia burgdorferi</i>, presents significant diagnostic challenges, often leading to delayed treatment and decreased therapeutic response to conventional antibiotics. This review aims to evaluate the potential of plant essential oils, known for their bacteriostatic, bactericidal, and anti-quorum sensing properties, as prophylactic, adjunct, or complementary treatments during the early stages of <i>B. burgdorferi</i> infection. The authors explore how these essential oils can target adaptive mechanisms and interactions of <i>B. burgdorferi</i>, including complement regulator-acquiring surface proteins (CRASPs), immune modulation, motility, chemotaxis, biofilm formation, efflux-pump mechanisms, and cyst formation. The authors identify current research gaps and propose frameworks to substantiate the clinical efficacy of essential oils for Lyme disease treatment. This review indicates that essential oils have multifaceted therapeutic potential and could provide a viable option for early intervention in Lyme disease. Further research is necessary to confirm their clinical applicability and safety.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"866-879"},"PeriodicalIF":2.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reverse Transcription-qPCR-Based Investigation of Antiviral miRNAs in Medicinal Plants.","authors":"Ilham Eroz Poyraz, Emel Sozen, Merve Saglam, Ismail Poyraz","doi":"10.1055/a-2660-1649","DOIUrl":"10.1055/a-2660-1649","url":null,"abstract":"<p><p>MicroRNAs (miRNAs) are endogenous regulators of gene expression that enable high adaptation to the living conditions of organisms. While plant antiviral miRNAs are effective in combating their viral pathogens, some plant miRNAs exhibit cross-kingdom interactions when targeting animal viruses. Current studies have reported that the miRNA contents of medicinal plants used in treating viral diseases are directly effective in combating the disease. Along with this study, the presence and expression levels of five plant miRNAs (miRNA765, miRNA954, miRNA1086, miRNA1328, and miRNA2911), known for their antiviral effects against human diseases, were analyzed in eight medicinal plants using the reverse transcription-quantitative real-time PCR (RT-qPCR) method. One of the test group medicinal plants was used as dry material for comparison purposes. <i>Triticum aestivum</i> L. fruits that contain high starch were preferred as the calibrator plant sample, and miRNA161 was used as the endogenous miRNA control. cDNAs were synthesized using stem-loop primers and amplified by RT-qPCR with SYBR green. Expression levels of antiviral miRNAs were analyzed using the fold change (Fc) and the relative quantification (RQ) data. At the end of this study, antiviral miRNAs were found in some medicinal plants and detected at high levels in preserved dried plant samples, such as <i>Viscum album</i> leaves.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"891-898"},"PeriodicalIF":2.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimycobacterial Activities of Cryptolepis sanguinolenta, Lantana camara, Zanthoxylum leprieurii Modeled as a Function of Their Fingerprints for Active Compounds Identification.","authors":"Naasson Tuyiringire, Deusdedit Tusubira, Samira Boarbi, Claude Mambo Muvunyi, Debby Mangelings, Yvan Vander Heyden","doi":"10.1055/a-2660-2042","DOIUrl":"10.1055/a-2660-2042","url":null,"abstract":"<p><p>There is a pressing need to discover novel anti-tuberculosis agents to combat emerging drug-resistant strains. <i>Cryptolepis sanguinolenta, Lantana camara</i>, and <i>Zanthoxylum leprieurii</i> have been identified as potential sources of anti-tuberculosis (TB) drug candidates. Previous studies have examined the metabolites and metabolic pathways in mycobacterial strains affected by methanolic extracts of these plants, but the specific active compounds responsible for the antimycobacterial activity, the effect on affected metabolites and metabolic pathways of mycobacterial cell cultures, remain unclear. Untargeted metabolic fingerprinting may help identify the active compounds. The objective of this study was to model the antimycobacterial activity of methanolic extracts of <i>C. sanguinolenta, L. camara</i>, and <i>Z. leprieurii</i> as a function of their UHPLC-MS fingerprints and determine whether specific peaks (compounds) in the fingerprints contributed significantly to the activity. In this study, fingerprints of 18 methanolic extracts from <i>C. sanguinolenta</i> roots, <i>L. camara</i> leaves, and <i>Z. leprieurii</i> stem barks were obtained with ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS). The minimal inhibitory concentrations (MICs) of these extracts against a pan-sensitive <i>M. tuberculosis</i> strain were determined using a resazurin-based microdilution assay. Fingerprints were processed and analyzed using regions of interest-multivariate curve resolution (ROIMCR). Partial least squares (PLS) regression was employed to model the MICs. Potential active compounds, including cryptolepine (from <i>C. sanguinolenta</i>), verbascoside (from <i>L. camara</i>), and isofagaridine (from <i>Z. leprieurii</i>), were identified as antimycobacterial compounds. These compounds likely influence mycobacterial metabolic processes, including cell wall synthesis, protein production, nucleotide metabolism, and energy generation. Further investigations are required to validate our findings.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"745-757"},"PeriodicalIF":2.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Systematic Review of Neuroprotective Effects of Mangosteen and its Xanthones Against Oxidative Stress and Inflammation.","authors":"Hin Yee Thew, Yong Chiang Tan, Yong Sze Ong, Bey Hing Goh, Kooi Yeong Khaw","doi":"10.1055/a-2665-2226","DOIUrl":"10.1055/a-2665-2226","url":null,"abstract":"<p><p>Mangosteen has garnered increasing attention for its medicinal properties against oxidative stress and inflammation-two major causative and progressive agents of neurodegenerative diseases. This systematic review explores the antioxidative and anti-inflammatory effects of mangosteen crude extracts and their purified bioactive compounds, highlighting their neuroprotective potential against neurodegenerative conditions.The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) strategy was used to identify studies published in English up to July 2024 across five databases (Cochrane, PubMed, Scopus, Web of Science, and Google Scholar). The Population, Intervention, Comparison, and Outcome (PICO) framework guided the search strategy, and duplicate records were removed using Covidence software. Of the 149 studies screened, 40 met the predefined inclusion criteria and were included in the review. The quality of the included studies was assessed using criteria adapted from the Cochrane Handbook, focusing on risk of bias and methodological rigor.Mangosteen extract and xanthones consistently reduced oxidative markers in various models. Anti-inflammatory effects were evident as mangosteen extract reduced pro-inflammatory cytokines and modulated the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-<i>κ</i>B) and cyclooxygenase-2 (COX-2) pathways in neuroinflammation models. Xanthones further suppressed inflammatory mediators and enhanced cellular resilience.The <i>in vitro</i> and <i>in vivo</i> results suggested the neuroprotective capabilities of mangosteen extracts and its purified bioactives. Despite that, gaps remain in understanding the potential synergistic effects of these bioactives, their druggability properties, and clinical applicability. Further research, especially clinical trials, will be necessary to further impel mangosteen and its derivatives into therapeutic applications.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"786-801"},"PeriodicalIF":2.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144964966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Local and Systemic Effects of Topical Betulinic Acid in a Psoriasis-like Inflammation Model in Mice.","authors":"Flávia S Fernandes, Gustavo S Silva, Rafael V Soares, Fabiana Ourique, Tiziane L D R Nakagawa, Daniel F Martins, Ariane Zamoner, Maicon R Kviecinski","doi":"10.1055/a-2644-8732","DOIUrl":"10.1055/a-2644-8732","url":null,"abstract":"<p><p>Psoriasis patients often discontinue oral and injectable treatments due to concerns about both safety and efficacy. Issues such as adverse side effects, limited long-term effectiveness, and fear of potential complications contribute to non-adherence, impacting treatment outcomes and patients' quality of life. Betulinic acid (BA) forms supramolecular aggregates through self-assembly in a hydroalcoholic vehicle, which was hypothesized to have antipsoriatic activity when applied topically. To test this, imiquimod was applied to induce psoriasis-like skin inflammation in mice (except in the untreated group) every 24 hours for 5 days. Two hours after each imiquimod application, the groups received either 100 µL of vehicle (10% glycerol aqueous solution), 0.05 mg/mL clobetasol (Clo), or 0.5 mg/mL BA. At the end of the study, the Psoriasis Area and Severity Index (PASI) was evaluated (n = 12/group), and complete skin clearance time (CSC) was determined in six mice per group. The remaining six mice per group were used to assess acanthosis, lymphocyte and granulocyte infiltration, and Akt and ERK phosphorylation in skin samples, as well as TNF<i>α</i>, IL-17A, IFN<i>γ</i>, and TGF<i>β</i> levels in serum. To assess treatment safety, we evaluated food and water intake, ambulation pattern, body weight gain, organ weights, and blood parameters. BA significantly reduced CSC time by up to 40% compared to the control and was 10% faster than Clo. Both BA and Clo reduced PASI and acanthosis to approximately one-third of control values, normalized immune cell infiltration and TNF<i>α</i> levels, decreased IL-17A by more than 30%, and reduced p-Akt and p-ERK2. BA uniquely normalized IFN<i>γ</i> levels without causing intolerable toxicity. Using an animal model of psoriatic skin inflammation, our findings support BA as a strong candidate for clinical translation, warranting further studies on its safety, pharmacokinetics, and optimal dosage in humans, potentially leading to randomized controlled trials in psoriasis patients.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"771-780"},"PeriodicalIF":2.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico and In vitro Screening of Medicinal Plants from Brazilian Traditional Medicine for Anti-Helicobacter pylori Activity.","authors":"Campili Mendes, Jéssica Raquel Borges Monteiro, Rodrigo de Almeida Romagna, Aliny Rodrigues de Jesus da Conceição, Pedro Henrique Tregnago, Amanda Eiriz Feu, Rita de Cássia Ribeiro Gonçalves, Warley de Souza Borges, Ricardo Machado Kuster, Rodrigo Rezende Kitagawa","doi":"10.1055/a-2635-4268","DOIUrl":"10.1055/a-2635-4268","url":null,"abstract":"<p><p>Medicinal plants and their phytocompounds are valuable shortcuts for discovering new, safer biologically active compounds or herbal medicines with reduced adverse effects. In this study, medicinal plant species were initially selected from Brazilian traditional medicine using a database of <i>in silico</i> and <i>in vitro</i> studies. A virtual screening study was carried out with a phytochemical database previously reported in the literature. The biological activity was evaluated <i>in silico</i> by PASS Online and molecular docking, then validated by <i>in vitro</i> anti-<i>Helicobacter pylori</i> assay. The chemical profile of the species was obtained by ESI(±)FT-ICR MS and LC-MS-DAD analysis. <i>Vernonia condensata, Bauhinia forficata, Jatropha gossypiifolia</i>, and <i>Sonalum paniculatum</i> were selected based on a survey of the literature for use of gastric diseases and anti-<i>Helicobacter pylori</i> potential. After PASS analysis, <i>Jatropha gossypiifolia</i> was selected for <i>in vitro</i> study because its compounds showed anti-<i>H. pylori</i> activity potential, inhibiting fumarate reductase enzyme. <i>J. gossypiifolia</i> extracted showed MIC of 64 µg/mL and MBC of 128 µg/mL in the <i>in vitro</i> anti-<i>H. pylori</i> assay. ESI(±)FT-ICR MS and LC-MS-DAD analysis showed compounds such as luteolin (1: ), isovitexin (2: ), luteolin-7-O-glucoside (3: ), isoorientin (4: ), and 3-genistein-8-C-glucoside (5: ). Molecular docking analysis showed a potential interaction of compounds in the enzyme active site such as hydrogen bonds with Arg404 and a similar interaction to fumaric acid, except for isoorientin (4: ). <i>J. gossypiifolia</i> showed promising activity and may represent a future alternative to treat <i>H. pylori</i> infections and their deleterious effects, reinforcing the therapeutic potential of this plant.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"837-849"},"PeriodicalIF":2.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144294784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory and Antioxidant Properties of Almond-Skin Acetonic Extract in Zebrafish (Danio rerio) Copper-Sulphate-induced Inflammation.","authors":"Rúben Barbosa, Ana Martins, Ivo Vaz Oliveira, Maria Inês Dias, Sandra M Monteiro, Carlos Venâncio, Luís Félix","doi":"10.1055/a-2654-9426","DOIUrl":"10.1055/a-2654-9426","url":null,"abstract":"<p><p>This study aimed to assess the properties of an acetone-based almond-skin (<i>Prunus dulcis</i>) extract and examine its effects on chemically induced inflammation by evaluating associated oxidative changes in an <i>in vivo</i> model. The phytochemical composition was analysed by LC-MS/MS while the antioxidant capacity was evaluated using DPPH, ABTS, and FRAP assays. The neutrophil migration was assessed using a CuSO₄-induced inflammation model in a transgenic zebrafish line (Tg(mpx : GFP)ⁱ¹¹⁴). Three days post-fertilisation zebrafish larvae were treated with 5 and 25 µg/mL extract for 1 h and then exposed to 10 µM CuSO₄ for 40 min. Following exposure, various biochemical markers were assessed, along with neurobehavioral effects. The polyphenolic profile revealed a high flavonoid content and good antioxidant properties confirmed by the DPPH, ABTS, and FRAP assays. The extract reduced neutrophil migration in a concentration-dependent manner, indicating anti-inflammatory activity. Additionally, it decreased reactive oxygen species (ROS) production and cell death without affecting mitochondrial membrane potential. The extract also mitigated oxidative damage, as shown by reduced malondialdehyde and protein carbonylation levels, alongside decreased antioxidant response markers. Neurobehavioral effects induced by CuSO₄ were alleviated, as evidenced by improved touch-evoked responses. These findings suggest that almond-skin extract holds significant antioxidant and anti-inflammatory properties, possibly through modulation of ROS and calcium signalling pathways.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"850-861"},"PeriodicalIF":2.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145207418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}