Planta medica最新文献

{"title":"Identification of Major Bioactive Anti-inflammatory Compounds of Derris scandens Stem Using RAW 264.7 Cells and HPLC-UV Analysis.","authors":"Worapol Sae-Foo, Gorawit Yusakul, Natsajee Nualkaew, Waraporn Putalun","doi":"10.1055/a-2192-2281","DOIUrl":"10.1055/a-2192-2281","url":null,"abstract":"<p><p><i>Derris scandens</i> (DS) is widely recognized for its therapeutic properties, specifically its analgesic effects, which significantly alleviate muscle pain. The chemical constituents of DS stem include various isoflavone derivatives. However, there is currently a lack of specified anti-inflammatory chemical markers and analytical methods for quality control. The present study aimed to evaluate the anti-inflammatory activity of DS and its constituents using the RAW 264.7 cell model. The expression of inflammatory genes such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-6 (IL-6), and 5-lipoxygenase (5-LOX) was examined using quantitative RT-PCR. An high-performance liquid chromatography with a UV detection method was developed to quantitatively analyze genistein-7-<i>O</i>-[<i>α</i>-rhamnopyranosyl-(1 → 6)]-<i>β</i>-glucopyranoside, genistein, derrisisoflavone A, lupalbigenin, and 6,8-diprenylgenistein in DS stem. The developed HPLC-UV method demonstrated high sensitivity with limits of detection and quantification ranging from 0.01 to 0.06 µg/mL and 0.03 to 0.18 µg/mL, respectively. The accuracy of the method ranged from 93.3 to 109.6%. Furthermore, the repeatability and reproducibility of the method were suitable, as indicated by the relative standard deviations of ≤ 3.02% and ≤ 6.22%, respectively. The DS extract notably inhibited NO production, exhibiting effects comparable to those of 500 µM diclofenac, and substantially suppressed the expression of iNOS, COX-2, IL-6, and 5-LOX of lipopolysaccharide (LPS)-induced genes. As to the pure isoflavone derivatives, the order of NO production inhibition was found to be genistein > lupalbigenin > derrisisoflavone A > 6,8-diprenylgenistein > genistein-7-<i>O</i>-[<i>α</i>-rhamnopyranosyl-(1 → 6)]-<i>β</i>-glucopyranoside. Genistein, derrisisoflavone A, and 6,8-diprenylgenistein significantly suppressed the upregulation of all LPS-induced genes. Consequently, these compounds are recommended as anti-inflammatory markers for the quantitative chemical analysis of DS.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"126-137"},"PeriodicalIF":2.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41237805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Potential of Berberine to Target Telocytes in Rabbit Heart.","authors":"Min Yang, Tong Yang, Lu Mei, Yingxing Zhang, Chunhua Liang, Xuebing Bai, Zhenwei Zhang, Yonghong Shi, Qiusheng Chen","doi":"10.1055/a-2176-5881","DOIUrl":"10.1055/a-2176-5881","url":null,"abstract":"<p><p>A brand-new class of interstitial cells, called telocytes, has been detected in the heart. Telocytes can connect and transmit signals to almost all cardiomyocytes; this is highly interrelated with the occurrence and development of heart diseases. Modern studies have shown that berberine has a therapeutic effect on cardiovascular health. However, berberine's mechanism of action on the cardiovascular system through cardiac telocytes is unclear. Interestingly, 5 µm of berberine remarkably decreased the concentration of intracellular calcium and membrane depolarization in cultured telocytes, upregulated the expression of CX43 and <i>β</i>-catenin, and downregulated the expressions of TRPV4 and TRPV1. Here, telocytes were identified in the vascular adventitia and intima, endocardium, myocardium, adventitia, and heart valves. Moreover, telocytes were broadly dispersed around cardiac vessels and interacted directly through gap junctions and indirectly through extracellular vesicles. Together, cardiac telocytes interact with berberine and then deliver drug information to the heart. Telocytes may be an essential cellular target for drug therapy of the cardiovascular system.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"84-95"},"PeriodicalIF":2.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10316805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tapirira guianensis is Selectively Cytotoxic, Induces Apoptosis to the Glioblastoma and Decreases Tumor Growth and Angiogenesis in vivo.","authors":"Ana Gabriela Silva Oliveira, Marina Andrade Rocha, Lucas Santos de Azevedo, Aline Thaynara de Moura Coelho, Rafael César Russo Chagas, Hélio Batista Santos, Ralph Gruppi Thomé, Peter Samuel, Evelyn Wolfram, Bonglee Kim, Rui Manuel Reis, Rosy Iara Maciel Azambuja Ribeiro","doi":"10.1055/a-2181-2569","DOIUrl":"10.1055/a-2181-2569","url":null,"abstract":"<p><p>Glioblastoma is the most frequent primary malignant brain tumor without effective treatment, which makes this work extremely relevant. The study of the bioactive compounds from medicinal plants plays an important role in the discovery of new drugs.This research investigated the constituents of <i>Tapirira guianensis</i> and its antitumor potential (<i>in vitro</i> and <i>in vivo)</i> in glioblastoma. The <i>T. guianensis</i> extracts were characterized by mass spectrometry. The ethyl acetate partition (01ID) and its fractions 01ID-F2 and 01ID-F4 from <i>T. guianensis</i> showed potential antitumor treatment evidenced by selective cytotoxicity for GAMG with IC50 14.1 µg/mL, 83.07 µg/mL, 59.27 µg/mL and U251 with IC50 25.92 µg/mL, 37.3 µg/mL and 18.84 µg/mL. Fractions 01ID-F2 and 01ID-F4 were 10 times more selective when compared to TMZ and 01ID for the two evaluated cell lines. <i>T. guianensis</i> also reduced matrix metalloproteinases 2 - 01ID-F2 (21.84%), 01ID-F4 (29.6%) and 9 - 01ID-F4 (73.42%), ID-F4 (53.84%) activities, and induced apoptosis mainly through the extrinsic pathway. Furthermore, all treatments significantly reduced tumor size (01ID p < 0,01, 01ID-F2 p < 0,01 and 01ID-F4 p < 0,0001) and caused blood vessels to shrink <i>in vivo</i>. The present findings highlight that <i>T. guianensis</i> exhibits considerable antitumor potential in preclinical studies of glioblastoma. This ability may be related to the phenolic compounds and sesquiterpene derivatives identified in the extracts. This study deserves further <i>in vivo</i> research, followed by clinical investigation.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"13-24"},"PeriodicalIF":2.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41208921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sargachromenol Attenuates Inflammatory Responses by Regulating NF-κB and Nrf2 Pathways in RAW 264.7 Cells and LPS-treated Mice.","authors":"Eun-Ji Joung, Min-Kyeong Lee, Minsup Lee, Misung Gwon, Taisun Shin, Heeyeon Ryu, Hyeon Hak Jeong, Myeong-Jin Kim, Ji Yun Van, Jae-Il Kim, Jinkyung Choi, Won-Kyo Jung, Hyeung-Rak Kim, Bonggi Lee","doi":"10.1055/a-2180-1338","DOIUrl":"10.1055/a-2180-1338","url":null,"abstract":"<p><p>This study aims to explore the anti-inflammatory mechanisms of sargachromenol in both RAW 264.7 cells and lipopolysaccharide (LPS)-treated mice, as previous reports have suggested that sargachromenol possesses anti-aging, anti-inflammatory, antioxidant, and neuroprotective properties. Although the precise mechanism behind its anti-inflammatory activity remains unclear, pretreatment with sargachromenol effectively reduced the production of nitric oxide, prostaglandin E₂, and interleukin (IL)-1<i>β</i> in LPS-stimulated RAW 264.7 cells by inhibiting cyclooxygenase-2. Moreover, sargachromenol inhibited the activation of nuclear factor-<i>κ</i>B (NF-<i>κ</i>B) by preventing the degradation of the inhibitor of <i>κ</i>B-<i>α</i> (I<i>κ</i>B-<i>α</i>) and inhibiting protein kinase B (Akt) phosphorylation in LPS-stimulated cells. We also found that sargachromenol induced the production of heme oxygenase-1 (HO-1) by activating the nuclear transcription factor erythroid-2-related factor 2 (Nrf2). In LPS-treated mice, oral administration of sargachromenol effectively reduced the levels of IL-1<i>β</i>, IL-6, and tumor necrosis factor-<i>α</i> (TNF-<i>α</i>) in the serum, suggesting its ability to suppress the production of inflammatory mediators by inhibiting the Akt/NF-<i>κ</i>B pathway and upregulating the Nrf2/HO-1 pathway.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"25-37"},"PeriodicalIF":2.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41237769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Morphology of Island and Inland Agastache rugosa and Their Gastroprotective Effects in EtOH/HCl-Induced Gastric Mucosal Gastritis.","authors":"Jun-Ho Song, Hyeon-Hwa Nam, Inkyu Park, Sungyu Yang, Jin Mi Chun, Yun-Soo Seo, Hyun-Yong Kim, Byeong Cheol Moon, Sohi Kang, Changjong Moon, Seong-Il Kang, Ji Hoon Song, Joong-Sun Kim","doi":"10.1055/a-2189-7272","DOIUrl":"10.1055/a-2189-7272","url":null,"abstract":"<p><p><i>Agastache rugosa</i> Kuntze (Lamiaceae; Labiatae), a medicinal and functional herb used to treat gastrointestinal diseases, grows well both on islands and inland areas in South Korea. Thus, we aimed to reveal the morphological and micromorphological differences between <i>A. rugosa</i> grown on island and inland areas and their pharmacological effects on gastritis in an animal model by combining morphological and mass spectrophotometric analyses. Morphological analysis showed that island <i>A. rugosa</i> had slightly smaller plants and leaves than inland plants; however, the density of all types of trichomes on the leaves, petioles, and stems of island <i>A. rugosa</i> was significantly higher than that of inland plants. The essential oil component analysis revealed that pulegone levels were substantially higher in island <i>A. rugosa</i> than in inland <i>A. rugosa</i>. Despite the differences between island and inland <i>A. rugosa</i>, treatment with both island and inland <i>A. rugosa</i> reduced gastric damages by more than 40% compared to the gastritis induction group. In addition, expression of inflammatory protein was reduced by about 30% by treatment of island and inland <i>A. rugosa.</i> The present study demonstrates quantitative differences in morphology and volatile components between island and inland plants; significant differences were not observed between the gastritis-inhibitory effects of island and inland <i>A. rugosa</i>, and the efficacy of island <i>A. rugosa</i> was found to be similar to that of <i>A. rugosa</i> grown in inland areas.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"4-12"},"PeriodicalIF":2.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71413519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Medicinal Cannabis: Extended Stability of Cannabis Extracts Produced Using a New Ethanol-based Extraction Method.","authors":"Camillo Morano, Michele Dei Cas, Eleonora Casagni, Marco Pallavicini, Cristiano Bolchi, Sara Penati, Arturo Cuomo, Marco Cascella, Sabrina Bimonte, Antonia Spensiero, Andrea Ferrari, Annunziata Lombardi, Gabriella Roda","doi":"10.1055/a-2189-8155","DOIUrl":"10.1055/a-2189-8155","url":null,"abstract":"<p><p>Cannabis as a therapeutic agent is increasing in popularity all around the globe, particularly in Western countries, and its potential is now well assessed. On the other hand, each country has its own regulation for the preparation of cannabis macerated oils; in Italy, there are only a few preparation methods allowed. With this work, we aim to perform a stability study of cannabis oils produced with a novel method for the extraction of cannabinoids from cannabis inflorescence. Three different varieties of cannabis were used, with and without the adding of tocopherol acetate as an antioxidant. Cannabinoids were extracted using ethanol at room temperature; then, the solvent was evaporated under reduced pressure and the preparations reconstituted with olive oil. In this work, we assessed the stability of both cannabinoids and terpenes in these formulas over 8 months. Cannabinoid stability was assessed by monitoring the concentrations of THC and CBD, while terpene stability was assessed by monitoring <i>β</i>-Caryophyllene and <i>α</i>-Humulene concentrations. Stability of the extracts was not influenced by the presence of tocopherol acetate, though refrigeration seems to be detrimental for a long storage of products, especially regarding THC concentrations. The improvements offered by this method reside in the flexibility in controlling the concentration of the extract and the ability to produce highly concentrated oils, alongside the possibility to produce standardized oils despite the variability of the starting plant material.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"73-80"},"PeriodicalIF":2.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"107592024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of the Daucus carota SMC-1 Cell Suspension Line for Poliovirus Vaccine Development.","authors":"Mónica Morales-Aguilar, Omayra Citlalli Bolaños-Martínez, Andrea Romero Maldonado, Dania O Govea-Alonso, Christian Carreño-Campos, María Luisa Villarreal, Sergio Rosales-Mendoza, Anabel Ortiz-Caltempa","doi":"10.1055/a-2181-2886","DOIUrl":"10.1055/a-2181-2886","url":null,"abstract":"<p><p>The development of virus-free, oral vaccines against poliovirus capable of inducing mucosal protective immunity is needed to safely combat this pathogen. In the present study, a carrot cell line expressing the poliovirus VP2 antigen was established at the level of callus and cell suspensions, exploring the effects of culture media (MS and B5), supplementation with urea, phytoregulators (2,4-D : KIN), and light conditions (continuous light, photoperiod, and total darkness). The best callus growth was obtained on B5 medium supplemented with 2 mg/L of 2,4-D + 2 mg/L kinetin and 0.0136 g/L of urea and in continuous light conditions. Suspension cultures of the SMC-1 line in 250 mL Erlenmeyer flasks had a maximum growth of 16.07 ± 0.03 g/L DW on day 12 with a growth rate of µ=0.3/d and a doubling time of 2.3 days. In a 2 L airlift bioreactor, the biomass yield achieved was 25.6 ± 0.05 g/L DW at day 10 with a growth rate of µ= 0.58/d and doubling time of 1.38 d. Cell growth was 1.5 times higher in bioreactors than in shake flasks, highlighting that both systems resulted in the accumulation of VP2 throughout the time in culture. The maximum VP2 yield in flasks was 387.8 µg/g DW at day 21, while in the reactor it was 550.2 µg/g DW at day 18. In conclusion, bioreactor-based production of the VP2 protein by the SMC-1 suspension cell line offers a higher productivity when compared to flask cultures, offering a key perspective to produce low-cost vaccines against poliomyelitis.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"63-72"},"PeriodicalIF":2.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49681072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination and Chemotaxonomic Analysis of Lanostane Triterpenoids in the Mycelia of Ganoderma spp. Using Ultra-performance Liquid Chromatography-Tandem Mass Spectrometry (I).","authors":"Yawen Yue, Shuai Zhou, Chilu Cheng, Liming Teng, Jingsong Zhang, Baokai Cui, Wei Han, Yucheng Dai, Na Feng","doi":"10.1055/a-2143-8357","DOIUrl":"10.1055/a-2143-8357","url":null,"abstract":"<p><p>A comprehensive and sensitive method combining ultra-performance liquid chromatography with tandem mass spectrometry was developed for the quantification of characteristic triterpenoids in <i>Ganoderma</i> mycelia. Eight ganoderic acids previously isolated from the mycelia of <i>Ganoderma lingzhi</i> were separated with a binary mobile phase on a reversed-phase C18 column. A triple quadrupole mass spectrometer equipped with an electrospray ionization source was used as the detector in the negative ion mode. Identification and quantitation of target ganoderic acids were accomplished using the dynamic multiple reaction monitoring mode. The developed method was validated in terms of linearity, precision, accuracy, stability, and recovery. The method was first applied to quantify the contents of eight ganoderic acids in the mycelia of <i>G. lingzhi</i> at different times to determine the optimum fermentation conditions. Subsequently, the distribution of triterpenoids and the contents of eight ganoderic acids in sixteen different <i>Ganoderma</i> species were investigated. The results indicated that UV chromatography combined with dynamic multiple reaction monitoring quantification was an effective chemotaxonomy method for <i>Ganoderma</i> species identification. This study also provided a helpful analytical methodology for both scientific and industrial applications in the quality control of <i>Ganoderma</i> triterpenoids.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"1505-1514"},"PeriodicalIF":2.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10054381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Consensus Statement on the Outcome of a Workshop on Paediatric Phytotherapy: Rationalising Optimal Dosing for Use in Children by Real-World Data.","authors":"Andreas Hensel, Rudolf Bauer, Michael Heinrich, Karin Kraft","doi":"10.1055/a-2177-7634","DOIUrl":"10.1055/a-2177-7634","url":null,"abstract":"","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"1442-1443"},"PeriodicalIF":2.1,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41183346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural Characterization and Anticomplement Activity of an Acidic Heteropolysaccharide from Lysimachia christinae Hance.","authors":"Zhou Hong, Li-Shuang Zhou, Zhi-Zhi Zhao, Guo-Qi Yuan, Xiao-Jiang Wang, Yan Lu, Dao-Feng Chen","doi":"10.1055/a-2148-7163","DOIUrl":"10.1055/a-2148-7163","url":null,"abstract":"<p><p>A novel acidic heteropolysaccharide (LCP-90-1) was isolated and purified from a traditional \"heat-clearing\" Chinese medicine, <i>Lysimachia christinae</i> Hance. LCP-90-1 (<i>Mw</i>, 20.65 kDa) was composed of Man, Rha, GlcA, Glc, Gal, and Ara, with relative molar ratios of 1.00: 3.00: 11.62: 1.31: 1.64: 5.24. The backbone consisted of 1,4-<i>α</i>-D-Glc<i>p</i>A, 1,4-<i>α</i>-D-Glc<i>p</i>, 1,4-<i>β</i>-L-Rha<i>p</i>, and 1,3,5-<i>α</i>-L-Ara<i>f</i>, with three branches of <i>β</i>-D-Gal<i>p</i>-(1 → 4)-<i>β</i>-L-Rha<i>p</i>-(1→, <i>α</i>-L-Ara<i>f</i>-(1→ and <i>α</i>-D-Man<i>p</i>-(1→ attached to the C-5 position of 1,3,5-<i>α</i>-L-Ara<i>f</i>. LCP-90-1 exhibited potent anticomplement activity (CH₅₀: 135.01 ± 0.68 µg/mL) <i>in vitro</i>, which was significantly enhanced with increased glucuronic acid (GlcA) content in its degradation production (LCP-90-1-A, CH₅₀: 28.26 ± 0.39 µg/mL). However, both LCP-90-1 and LCP90-1-A were inactivated after reduction or complete acid hydrolysis. These observations indicated the important role of GlcA in LCP-90-1 and associated derivatives with respect to anticomplement activity. Similarly, compared with LCP-90-1, the antioxidant activity of LCP-90-1-A was also enhanced. Thus, polysaccharides with a high content of GlcA might be important and effective substances of <i>L. christinae</i>.</p>","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"1457-1467"},"PeriodicalIF":2.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10431765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}