Planta medica最新文献_第9页

Impact of Phylogenetically Diverse Bacterial Endophytes of Bergenia pacumbis on Bergenin Production in the Plant Cell Suspension Cultures. 荚蒾的系统发育多样性细菌内生菌对植物细胞悬浮培养液中荚蒾苷产量的影响

IF 2.1 4区医学

Planta medica Pub Date : 2024-06-01 Epub Date: 2023-09-06 DOI: 10.1055/a-2162-4018

Christoph Wawrosch, Martina Oberhofer, Stefan Steinbrecher, Sergey B Zotchev

{"title":"Impact of Phylogenetically Diverse Bacterial Endophytes of Bergenia pacumbis on Bergenin Production in the Plant Cell Suspension Cultures.","authors":"Christoph Wawrosch, Martina Oberhofer, Stefan Steinbrecher, Sergey B Zotchev","doi":"10.1055/a-2162-4018","DOIUrl":"10.1055/a-2162-4018","url":null,"abstract":"Plant in vitro cultures are potential sources for secondary metabolites. However, low productivity is often a major drawback for industrial application. Elicitation is an important strategy to improve product formation in vitro. In this context, endophytes are of special interest as biotic elicitors due to their possible interaction with the metabolism of the host plant. A total of 128 bacterial endophytes were isolated from the medicinal plant Bergenia pacumbis and taxonomically classified using 16S rRNA gene sequencing. Five strains belonging to different genera were grown in lysogeny broth and tryptic soy broth medium and cells as well as spent media were used as elicitors in cell suspension cultures of B. pacumbis. Production of the main bioactive compound bergenin was enhanced 3-fold (964 µg/g) after treatment with cells of Moraxella sp. or spent tryptic soy broth medium of Micrococcus sp. These results indicate that elicitation of plant cell suspension cultures with endophytic bacteria is a promising strategy for enhancing the production of desired plant metabolites.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"651-657"},"PeriodicalIF":2.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10167459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Metabolomic Approach to Investigate the Effect of Phytonutrients on Proteostasis and Metabolic Pathways in Drosophila melanogaster. 用代谢组学方法研究植物营养素对黑腹果蝇蛋白稳态和代谢途径的影响

IF 2.7 4区医学

Planta medica Pub Date : 2024-06-01 Epub Date: 2024-06-06 DOI: 10.1055/a-2192-3167

Eleni V Mikropoulou, Zoi Evangelakou, Ioannis P Trougakos, Maria Halabalaki

{"title":"A Metabolomic Approach to Investigate the Effect of Phytonutrients on Proteostasis and Metabolic Pathways in Drosophila melanogaster.","authors":"Eleni V Mikropoulou, Zoi Evangelakou, Ioannis P Trougakos, Maria Halabalaki","doi":"10.1055/a-2192-3167","DOIUrl":"https://doi.org/10.1055/a-2192-3167","url":null,"abstract":"The use of Drosophila melanogaster as a biological platform to study the effect of diet and food bioactives on the metabolome remains a highly unexplored subject. Aiming to establish alternative solutions for the investigation of nutritional interventions with bioactive natural products by employing LC-MS-based metabolomics approaches, we assessed the effect of a phytonutrient-rich extract from the endemic Mediterranean plant Cichorium spinosum (stamnagkàthi) on a Drosophila population. The extract's modulating effect on the proteostasis network and metabolism of young D. melanogaster flies was evaluated. Furthermore, an untargeted metabolomics approach, employing a C18 UPLC-ESI-Orbitrap-HRMS/MS platform, permitted the detection of several biomarkers in the metabolic profile of Drosophila's tissues; while targeted amino acid quantification in Drosophila tissue was simultaneously performed by employing aTRAQ labeling and an ion-pairing UPLC-ESI-SWATH-HRMS/MS platform. The detected metabolites belong to different chemical classes, and statistical analysis with chemometrics tools was utilized to reveal patterns and trends, as well as to uncover potential class-distinguishing features and possible biomarkers. Our findings suggest that Drosophila can serve as a valuable in vivo model for investigating the role of bioactive phytoconstituents, like those found in C. spinosum's decoction, on diverse metabolic processes. Additionally, the fruit fly represents a highly effective platform to investigate the molecular mechanisms underlying sex differences in diverse aspects of nutrition and physiology in higher metazoans.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":"90 7-08","pages":"512-522"},"PeriodicalIF":2.7,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141284462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxidation Study of Oleocanthal and Oleacein Induced by Oxone. 氧化酮诱导的油菜醛和油菜素氧化研究

IF 2.7 4区医学

Planta medica Pub Date : 2024-06-01 Epub Date: 2024-06-06 DOI: 10.1055/a-2235-0009

Lemonia Antoniadi, Apostolis Angelis, Ioannis K Kostakis, Leandros A Skaltsounis

引用次数: 0

Special Issue Celebrating the Austrian Pharmacognosy. 庆祝奥地利药物学特刊》。

IF 2.1 4区医学

Planta medica Pub Date : 2024-06-01 Epub Date: 2024-06-06 DOI: 10.1055/a-2285-6455

引用次数: 0

iso-Guttiferone J and Structure Revision of Guttiferone J from Garcinia gummi-gutta: A Combined Experimental and Integrated QM/NMR Approach. 通过实验和 QM/NMR 综合方法对来自藤黄属植物 gummi-gutta 的 iso-Guttiferone J 和 Guttiferone J 的结构进行修正：实验和 QM/NMR 综合方法。

IF 2.7 4区医学

Planta medica Pub Date : 2024-06-01 Epub Date: 2024-06-06 DOI: 10.1055/a-2232-4755

Pankaj Pandey, Mantasha Idrisi, Zulfiqar Ali, Islam Husain, William M Neal, Shabana I Khan, Daneel Ferreira, Amar G Chittiboyina, Ikhlas A Khan

{"title":"iso-Guttiferone J and Structure Revision of Guttiferone J from Garcinia gummi-gutta: A Combined Experimental and Integrated QM/NMR Approach.","authors":"Pankaj Pandey, Mantasha Idrisi, Zulfiqar Ali, Islam Husain, William M Neal, Shabana I Khan, Daneel Ferreira, Amar G Chittiboyina, Ikhlas A Khan","doi":"10.1055/a-2232-4755","DOIUrl":"https://doi.org/10.1055/a-2232-4755","url":null,"abstract":"Many polyprenylated acylphloroglucinols with fascinating chemical structures and intriguing biological activities have been identified as key to phytochemicals isolated from Garcinia, Hypericum, and related genera. In the present work, two chiral, tautomeric, highly-oxygenated polyprenylated acylphloroglucinols tethered with acyl and prenyl moieties on a bicyclo[3.3.1]nonanetrione core were isolated from the 95% ethanolic extract of Garcinia gummi-gutta fruit. The structures of both compounds were elucidated based on the NMR and MS data with ambiguity in the exact position of the enol and keto functions at C-1 and C-3 of the core structure. The structures of both polyprenylated acylphloroglucinols were established as a structurally revised guttiferone J and the new iso-guttiferone J with the aid of gauge-independent atomic orbital NMR calculations, CP3 probability analyses, specific rotation calculations, and electronic circular dichroism calculations in combination with the experimental data. The structures of both compounds resemble hyperforin, a potent activator of the human pregnane X receptor. As expected, both compounds showed strong pregnane X receptor activation at 10 µM [7.1-fold (guttiferone J) and 5.0-fold (iso-guttiferone J)], explained by a molecular docking study, necessitating further in-depth investigation to substantiate the herb-drug interaction potential of G. gummi-gutta upon co-administration with pharmaceutical drugs.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":"90 7-08","pages":"631-640"},"PeriodicalIF":2.7,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141284466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

VOLKSMED Database: A Source for Forgotten Wound Healing Plants in Austrian Folk Medicine. VOLKSMED 数据库：奥地利民间医药中被遗忘的伤口愈合植物资料库。

IF 2.7 4区医学

Planta medica Pub Date : 2024-06-01 Epub Date: 2024-06-06 DOI: 10.1055/a-2225-7545

Elisabeth Eichenauer, Johannes Saukel, Sabine Glasl

{"title":"VOLKSMED Database: A Source for Forgotten Wound Healing Plants in Austrian Folk Medicine.","authors":"Elisabeth Eichenauer, Johannes Saukel, Sabine Glasl","doi":"10.1055/a-2225-7545","DOIUrl":"10.1055/a-2225-7545","url":null,"abstract":"The global increase in the incidence of wounds is concerning and fuels the search for new treatment options. The use of traditional medicinal plants in wound healing represents an appreciated available therapeutic possibility. This work introduces the VOLKSMED database, which contains plants and other materials used in Austrian folk medicine, either as monographs or mixtures. This work focuses on the monographs of the database. Concerning wound healing, Hypericum sp., Arnica montana, Calendula officinalis, Plantago sp., and Malva sp. are the most commonly used plants. The focus of this paper is set on selected lesser-known plants (Abies alba, Anthyllis vulneraria, Brassica sp., Gentiana sp., Larix decidua, Picea abies, Sambucus sp., Sanicula europaea) and their status quo in literature concerning wound healing. A systematic search using the databases SciFinder, SCOPUS, and PubMed yielded substantial evidence for the wound healing potential of Brassica sp., Gentiana sp., the Pinaceae A. abies, L. decidua, and P. abies, as well as Sambucus nigra. In vivo and clinical studies substantiate their use in Austrian folk medicine. According to the literature, especially A. vulneraria, Sambucus racemosa, and S. europaea would be worth investigating in-depth since data concerning their wound healing effects - even though scarce - are convincing. In conclusion, the VOLKSMED database contains promising opportunities for further treatment options in the field of wound healing. Future research should consider the listed plants to support their traditional use in Austrian folk medicine and possibly promote the implementation of old knowledge in modern medicine.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":"90 7-08","pages":"498-511"},"PeriodicalIF":2.7,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141284474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Withanolide Profile and Acetylcholinesterase Inhibitory Activity of Two Argentinean Jaborosa Species. 两种阿根廷茉莉品种的 Withanolide 特征和乙酰胆碱酯酶抑制活性。

IF 2.1 4区医学

Planta medica Pub Date : 2024-06-01 Epub Date: 2024-06-06 DOI: 10.1055/a-2255-7096

Natalia P Alza, Eva M Pferschy-Wenzig, Olaf Kunert, Ana P Murray

引用次数: 0

Increased Glycolytic Activity Is Part of Impeded M1(LPS) Macrophage Polarization in the Presence of Urolithin A. 在尿石素 A 的作用下，糖酵解活性增加是 M1（LPS）巨噬细胞极化受阻的部分原因。

IF 2.1 4区医学

Planta medica Pub Date : 2024-06-01 Epub Date: 2024-06-06 DOI: 10.1055/a-2240-7462

Sheyda Bahiraii, Barbara Braunböck-Müller, Elke H Heiss

{"title":"Increased Glycolytic Activity Is Part of Impeded M1(LPS) Macrophage Polarization in the Presence of Urolithin A.","authors":"Sheyda Bahiraii, Barbara Braunböck-Müller, Elke H Heiss","doi":"10.1055/a-2240-7462","DOIUrl":"10.1055/a-2240-7462","url":null,"abstract":"Urolithin A is a gut metabolite of ellagitannins and reported to confer health benefits, e.g., by increased clearance of damaged mitochondria by macroautophagy or curbed inflammation. One targeted cell type are macrophages, which are plastic and able to adopt pro- or anti-inflammatory polarization states, usually assigned as M1 and M2 macrophages, respectively. This flexibility is tightly coupled to characteristic shifts in metabolism, such as increased glycolysis in M1 macrophages, and protein expression upon appropriate stimulation. This study aimed at investigating whether the anti-inflammatory properties of U: rolithin A may be driven by metabolic alterations in cultivated murine M1(lipopolysaccharide) macrophages. Expression and extracellular flux analyses showed that urolithin A led to reduced il1β, il6, and nos2 expression and boosted glycolytic activity in M1(lipopolysaccharide) macrophages. The pro-glycolytic feature of UROLITHIN A: occurred in order to causally contribute to its anti-inflammatory potential, based on experiments in cells with impeded glycolysis. Mdivi, an inhibitor of mitochondrial fission, blunted increased glycolytic activity and reduced M1 marker expression in M1(lipopolysaccharide/UROLITHIN A: ), indicating that segregation of mitochondria was a prerequisite for both actions of UROLITHIN A: . Overall, we uncovered a so far unappreciated metabolic facet within the anti-inflammatory activity of UROLITHIN A: and call for caution about the simplified notion of increased aerobic glycolysis as an inevitably proinflammatory feature in macrophages upon exposure to natural products.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":"90 7-08","pages":"546-553"},"PeriodicalIF":2.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141284465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant-Derived Peptides: (Neglected) Natural Products for Drug Discovery. 植物肽：用于药物发现的（被忽视的）天然产品。

IF 2.7 4区医学

Planta medica Pub Date : 2024-06-01 Epub Date: 2024-06-06 DOI: 10.1055/a-2219-9724

Christian W Gruber

引用次数: 0

Statistical Experimental Designs for cLTB-Syn Vaccine Production Using Daucus carota Cell Suspension Cultures 利用菊苣细胞悬浮培养液生产 cLTB-Syn 疫苗的统计实验设计

IF 2.7 4区医学

Planta medica Pub Date : 2024-05-02 DOI: 10.1055/a-2307-0400

{"title":"Statistical Experimental Designs for cLTB-Syn Vaccine Production Using Daucus carota Cell Suspension Cultures","authors":"","doi":"10.1055/a-2307-0400","DOIUrl":"https://doi.org/10.1055/a-2307-0400","url":null,"abstract":"The carrot-made LTB-Syn antigen (cLTB-Syn) is a vaccine candidate against synucleinopathies based on carrot cells expressing the target antigen LTB and syn epitopes. Therefore, the development of an efficient production process is required with media culture optimization to increase the production yields as the main goal. In this study, the effect of two nitrogen sources (urea and glutamate) on callus cultures producing cLTB-Syn was studied, observing that the addition of 17 mM urea to MS medium favored the biomass yield. To optimize the MS media composition, the influence of seven medium components on biomass and cLTB-Syn production was first evaluated by a Plackett–Burman design (PBD). Then, three factors were further analyzed using a central composite design (CCD) and response surface methodology (RSM). The results showed a 1.2-fold improvement in biomass, and a 4.5-fold improvement in cLTB-Syn production was achieved at the shake-flask scale. At the bioreactor scale, there was a 1.5-fold increase in biomass and a 2.8-fold increase in cLTB-Syn yield compared with the standard MS medium. Moreover, the cLTB-Syn vaccine induced humoral responses in BALB/c mice subjected to either oral or subcutaneous immunization. Therefore, cLTB-Syn is a promising vaccine candidate that will aid in developing immunotherapeutic strategies to combat PD and other neurodegenerative diseases without the need for cold storage, making it a financially viable option for massive immunization.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":"12 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140841757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0