Planta medica最新文献_第9页

Discovery and Characterization of Panaxatriol as a Novel Thrombin Inhibitor from Panax notoginseng Using a Combination of Computational and Experimental Approaches. 利用计算和实验相结合的方法从三七中发现新型凝血酶抑制剂三七三醇并确定其特性。

IF 2.1 4区医学

Planta medica Pub Date : 2024-08-01 Epub Date: 2024-06-05 DOI: 10.1055/a-2339-2720

Xing Wang, Yuqing Ma, Chunfang Zuo, Zixi Zhao, Ruonan Ma, Lele Wang, Yuzhen Fang, Yuxin Zhang, Xia Wu

{"title":"Discovery and Characterization of Panaxatriol as a Novel Thrombin Inhibitor from Panax notoginseng Using a Combination of Computational and Experimental Approaches.","authors":"Xing Wang, Yuqing Ma, Chunfang Zuo, Zixi Zhao, Ruonan Ma, Lele Wang, Yuzhen Fang, Yuxin Zhang, Xia Wu","doi":"10.1055/a-2339-2720","DOIUrl":"10.1055/a-2339-2720","url":null,"abstract":"Thrombin is a crucial enzyme in the coagulation cascade, and inhibitors of thrombin have been extensively studied as potential antithrombotic agents. The objective of this study was to identify natural inhibitors of thrombin from Panax notoginseng and evaluate their biological activity in vitro and binding characteristics. A combined approach involving molecular docking, thrombin inhibition assays, surface plasmon resonance, and molecular dynamics simulation was utilized to identify natural thrombin inhibitors. The results demonstrated that panaxatriol directly inhibits thrombin, with an IC50 of 10.3 µM. Binding studies using surface plasmon resonance revealed that panaxatriol interacts with thrombin, with a KD value of 7.8 µM. Molecular dynamics analysis indicated that the thrombin-panaxatriol system reached equilibrium rapidly with minimal fluctuations, and the calculated binding free energy was - 23.8 kcal/mol. The interaction between panaxatriol and thrombin involves the amino acid residues Glu146, Glu192, Gly216, Gly219, Tyr60A, and Trp60D. This interaction provides a mechanistic basis for further optimizing panaxatriol as a thrombin inhibitor. Our study has shown that panaxatriol serves as a direct thrombin inhibitor, laying the groundwork for further research and development of novel thrombin inhibitors.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"801-809"},"PeriodicalIF":2.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141262410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cannabigerol and Cannabicyclol Block SARS-CoV-2 Cell Fusion. 大麻萜酚和大麻双环酚能阻止 SARS-CoV-2 细胞融合

IF 2.1 4区医学

Planta medica Pub Date : 2024-08-01 Epub Date: 2024-06-17 DOI: 10.1055/a-2320-8822

Nica Classen, Thanet Pitakbut, Michael Schöfbänker, Joachim Kühn, Eike R Hrincius, Stephan Ludwig, Andreas Hensel, Oliver Kayser

{"title":"Cannabigerol and Cannabicyclol Block SARS-CoV-2 Cell Fusion.","authors":"Nica Classen, Thanet Pitakbut, Michael Schöfbänker, Joachim Kühn, Eike R Hrincius, Stephan Ludwig, Andreas Hensel, Oliver Kayser","doi":"10.1055/a-2320-8822","DOIUrl":"10.1055/a-2320-8822","url":null,"abstract":"The search for new active substances against SARS-CoV-2 is still a central challenge after the COVID-19 pandemic. Antiviral agents to complement vaccination are an important pillar in the clinical situation. Selected cannabinoids such as cannabigerol, cannabicyclol, cannabichromene, and cannabicitran from Cannabis sativa and synthetic homologues of cannabigerol and cannabicyclol were evaluated for effects on the cell viability of Vero cells (CC50 of cannabigerol and cannabicyclol 40 resp. 38 µM) and reduced virus entry of vesicular stomatitis pseudotyped viruses with surface-expressed SARS-CoV-2 spike protein at 20 µM. In addition to a reduction of pseudotyped virus entry, a titer reduction assay on Vero cells after preincubation of Wuhan SARS-CoV-2 significantly confirmed antiviral activity. Investigations on the molecular targets addressed by cannabigerol and cannabicyclol indicated that both compounds are inhibitors of SARS-CoV-2 spike protein-mediated membrane fusion, as could be shown by a virus-free reporter fusion inhibition assay (EC50 for cannabigerol 5.5 µM and for cannabicyclol 10.8 µM) and by monitoring syncytia formation in Vero reporter cells. Selectivity indices were calculated as 7.4 for cannabigerol and 3.5 for cannabicyclol. Systematic semisynthetic alterations of cannabigerol and cannabicyclol indicated that the side chains of both compounds do not contribute to the observed anti-membrane fusion activity.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"717-725"},"PeriodicalIF":2.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141420385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evidence of Urtica dioica Agglutinin's Antiproliferative and Anti-migratory Potentials on the Hyaluronic Acid-Overexpressing Prostate Cancer Cells. 荨麻凝集素对透明质酸过度表达的前列腺癌细胞具有抗增殖和抗迁移潜能的证据

IF 2.1 4区医学

Planta medica Pub Date : 2024-08-01 Epub Date: 2024-06-28 DOI: 10.1055/a-2324-2250

Mohammadkazem Heydari, Abasalt Hosseinzadeh Colagar, Davood Sabour, Hamid Reza Khorasani

{"title":"Evidence of Urtica dioica Agglutinin's Antiproliferative and Anti-migratory Potentials on the Hyaluronic Acid-Overexpressing Prostate Cancer Cells.","authors":"Mohammadkazem Heydari, Abasalt Hosseinzadeh Colagar, Davood Sabour, Hamid Reza Khorasani","doi":"10.1055/a-2324-2250","DOIUrl":"10.1055/a-2324-2250","url":null,"abstract":"Hyaluronic acid is composed of repeating sugar units, glucuronic acid and N-acetylglucosamine, which are often associated with increased tumor progression. Urtica dioica agglutinin is a potential component that exhibits a high affinity for binding to N-acetylglucosamine. This study aimed to investigate U. dioica Agglutinin's potential to inhibit the proliferation and migration of prostate cancer cells with high expression of hyaluronic acid through molecular docking and in vitro studies. The expression of hyaluronan synthase genes in prostate tissue and cell lines was checked by an in silico study, and the interaction between hyaluronic acid with both CD44 transmembrane glycoprotein and U. dioica agglutinin was analyzed through molecular docking. U. dioica Agglutinin's effect on cell viability (neutral red uptake assay), migration (scratch wound healing assays), and both CD44 and Nanog expression (quantitative real-time polymerase chain reaction) were assessed in vitro. The results showed that in prostate cancer cell lines, the PC3 cell line has the highest expression of hyaluronan synthase genes. U. dioica agglutinin exhibits an interaction of six specific residues on CD44 compared to hyaluronic acid's singular residue. While U. dioica agglutinin alone effectively reduced cell viability and wound closer (≥ 150 µg/mL), combining it with hyaluronic acid significantly shifted the effective concentration to a higher dose (≥ 350 µg/mL). These results, together with low Nanog and high CD44 gene expression, suggest that U. dioica agglutinin may impair the CD44-HA pathway in PC3 cells. This possibility is supported by U. dioica Agglutinin's ability to compete with hyaluronic acid for binding to CD44. Based on this, U. dioica agglutinin as a plant lectin shows promise in inhibiting cancer proliferation and migration by targeting its dependence on hyaluronic acid.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"774-784"},"PeriodicalIF":2.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141470128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Approaches for the Analysis and Isolation of Benzylisoquinoline Alkaloids in Chelidonium majus. 分析和分离大叶诃子中苄基异喹啉生物碱的新方法。

IF 2.7 4区医学

Planta medica Pub Date : 2024-06-01 Epub Date: 2024-06-06 DOI: 10.1055/a-2204-5686

Michael Zwerger, Lynda Boeck, Julia Manzl, Stefan Schwaiger, Markus Ganzera

{"title":"Novel Approaches for the Analysis and Isolation of Benzylisoquinoline Alkaloids in Chelidonium majus.","authors":"Michael Zwerger, Lynda Boeck, Julia Manzl, Stefan Schwaiger, Markus Ganzera","doi":"10.1055/a-2204-5686","DOIUrl":"10.1055/a-2204-5686","url":null,"abstract":"Benzylisoquinoline alkaloids are the major bioactive components in Chelidonium majus, a plant that has a long usage history for the treatment of gastrointestinal ailments in European and Asian phytomedicine. This study reports on the development and application of a supercritical fluid chromatography technique for the simultaneous qualitative and quantitative determination of seven benzylisoquinoline alkaloids in under six minutes using a Viridis BEH 2-EP column and a modifier comprising methanol with 30% acetonitrile and 20 mM ammonium formate. The method was fully validated according to ICH guidelines showing, e.g., excellent linearity (≥ 0.9997) and maximum deviations for intraday and inter-day precision of 2.99 and 2.76%, respectively. The new supercritical fluid chromatography assay was not only employed for the analysis of several C. majus samples but was also used for the subsequent development of a fast centrifugal partition chromatography technique, whereby five benzylisoquinoline alkaloids could be isolated within approximately 2.5 h, with only two of them, protopine and chelidonine, requiring an additional purification step. To achieve this, a solvent system composed of chloroform/methanol/0.3 M hydrochloric acid was used in descending mode. By injecting 500 mg of crude extract, stylopine (1.93 mg), sanguinarine (0.57 mg), chelidonine (1.29 mg), protopine (1.95 mg), and coptisine (7.13 mg) could be obtained. The purity of compounds was confirmed by supercritical fluid chromatography and MS.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":"90 7-08","pages":"523-533"},"PeriodicalIF":2.7,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141284467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variability of Chalcone Synthase in Chamomile Accessions (Matricaria chamomilla). 洋甘菊品种（Matricaria chamomilla）中查尔酮合成酶的变异性。

IF 2.7 4区医学

Planta medica Pub Date : 2024-06-01 Epub Date: 2024-06-06 DOI: 10.1055/a-2214-0940

Melanie Novak, Dijana Jovanovic, Johannes Novak

{"title":"Variability of Chalcone Synthase in Chamomile Accessions (Matricaria chamomilla).","authors":"Melanie Novak, Dijana Jovanovic, Johannes Novak","doi":"10.1055/a-2214-0940","DOIUrl":"https://doi.org/10.1055/a-2214-0940","url":null,"abstract":"Chamomile (Matricaria chamomilla) is an important medicinal plant whose beneficial activities partly rely on certain flavonoids. The first dedicated step in flavonoid biosynthesis is chalcone synthase (CHS, EC 2.3.1.74). The genomic DNA of CHS was studied in six chamomile specimens from different genotypes to describe interspecimen, as well as interspecific, variability. One specimen of M. discoidea was included as an outgroup. The two exons of CHS of M. chamomilla (McCHS) and M. discoidea (MdCHS) were 188 bp and 1,011 bp long, separated by an intron of variable length between 192 and 199 bp in McCHS and 201 bp in MdCHS, respectively. The two exons with 5.3 and 6.2 mutations per 100 bp, respectively, were more conserved than the intron with 11.5 mutations per 100 bp. In total, 96 SNPs were detected in both species, of which 12 SNPs were only present in MdCHS and 80 SNPs only in McCHS. Overall, 70 haplotypes (multilocus genotypes, MLGs) were detected. The samples could be classified into two groups, a 'compact' group of a low number and diversity of haplotypes and a 'variable' group of a high number and diversity of haplotypes. Of the 74 SNPs in McCHS, only six SNPs were non-synonymous. However, the amino acid changes did not affect critical areas of the enzyme. The combination of the six SNPs resulted in nine translated amino acid MLGs. The CHS network located MdCHS, due to the crossing barrier, quite distant from chamomile. MdCHS docked to McCHS at a position from where McCHS divergently evolved into two directions.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":"90 7-08","pages":"534-545"},"PeriodicalIF":2.7,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141284473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flower Power: An Overview on Chemistry and Biological Impact of Selected Essential Oils from Blossoms. 花的力量精选花朵精油的化学和生物影响概述。

IF 2.7 4区医学

Planta medica Pub Date : 2024-06-01 Epub Date: 2024-06-06 DOI: 10.1055/a-2215-2791

Sinah Prosche, Iris Stappen

{"title":"Flower Power: An Overview on Chemistry and Biological Impact of Selected Essential Oils from Blossoms.","authors":"Sinah Prosche, Iris Stappen","doi":"10.1055/a-2215-2791","DOIUrl":"10.1055/a-2215-2791","url":null,"abstract":"Natural raw materials such as essential oils have received more and more attention in recent decades, whether in the food industry, as flavorings and preservatives, or as insecticides and insect repellents. They are, furthermore, very popular as fragrances in perfumes, cosmetics, and household products. In addition, aromatherapy is widely used to complement conventional medicine. This review summarizes investigations on the chemical composition and the most important biological impacts of essential oils and volatile compounds extracted from selected aromatic blossoms, including Lavandula angustifolia, Matricaria recutita, Rosa x damascena, Jasminum grandiflorum, Citrus x aurantium, Cananga odorata, and Michelia alba. The literature was collected from PubMed, Google Scholar, and Science Direct. Blossom essential oils discussed in this work are used in a wide variety of clinical issues. The application is consistently described as safe in studies and meta-analyses, although there are notes that using essential oils can also have side effects, especially dermatologically. However, it can be considered as confirmed that essential oils have positive influences on humans and can improve quality of life in patients with psychiatric disorders, critically ill patients, and patients in other exceptional situations. Although the positive effect of essential oils from blossoms has repeatedly been reported, evidence-based clinical investigations are still underrepresented, and the need for research is demanded.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":"90 7-08","pages":"595-626"},"PeriodicalIF":2.7,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141284464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Light-activated Effect of Natural Anthraquinone Parietin against Candida auris and Other Fungal Priority Pathogens. 天然蒽醌 Parietin 对白色念珠菌和其他真菌重点病原体的光激活效应

IF 2.7 4区医学

Planta medica Pub Date : 2024-06-01 Epub Date: 2024-06-06 DOI: 10.1055/a-2249-9110

Johannes Fiala, Thomas Roach, Andreas Holzinger, Yurii Husiev, Lisa Delueg, Fabian Hammerle, Eva Sanchez Armengol, Harald Schöbel, Sylvestre Bonnet, Flavia Laffleur, Ilse Kranner, Michaela Lackner, Bianka Siewert

{"title":"The Light-activated Effect of Natural Anthraquinone Parietin against Candida auris and Other Fungal Priority Pathogens.","authors":"Johannes Fiala, Thomas Roach, Andreas Holzinger, Yurii Husiev, Lisa Delueg, Fabian Hammerle, Eva Sanchez Armengol, Harald Schöbel, Sylvestre Bonnet, Flavia Laffleur, Ilse Kranner, Michaela Lackner, Bianka Siewert","doi":"10.1055/a-2249-9110","DOIUrl":"10.1055/a-2249-9110","url":null,"abstract":"Antimicrobial photodynamic therapy (aPDT) is an evolving treatment strategy against human pathogenic microbes such as the Candida species, including the emerging pathogen C. auris. Using a modified EUCAST protocol, the light-enhanced antifungal activity of the natural compound parietin was explored. The photoactivity was evaluated against three separate strains of five yeasts, and its molecular mode of action was analysed via several techniques, i.e., cellular uptake, reactive electrophilic species (RES), and singlet oxygen yield. Under experimental conditions (λ = 428 nm, H = 30 J/cm2, PI = 30 min), microbial growth was inhibited by more than 90% at parietin concentrations as low as c = 0.156 mg/L (0.55 µM) for C. tropicalis and Cryptococcus neoformans, c = 0.313 mg/L (1.10 µM) for C. auris, c = 0.625 mg/L (2.20 µM) for C. glabrata, and c = 1.250 mg/L (4.40 µM) for C. albicans. Mode-of-action analysis demonstrated fungicidal activity. Parietin targets the cell membrane and induces cell death via ROS-mediated lipid peroxidation after light irradiation. In summary, parietin exhibits light-enhanced fungicidal activity against all Candida species tested (including C. auris) and Cryptococcus neoformans, covering three of the four critical threats on the WHO's most recent fungal priority list.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":"90 7-08","pages":"588-594"},"PeriodicalIF":2.7,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141284472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of 3Rs in Caenorhabditis elegans Research for the Identification of Health-Promoting Natural Products. 在草履虫研究中应用 3Rs 鉴定促进健康的天然产品。

IF 2.1 4区医学

Planta medica Pub Date : 2024-06-01 Epub Date: 2024-06-06 DOI: 10.1055/a-2254-0131

Martina Redl, Anusha Shayegan, Judith M Rollinger

{"title":"Application of 3Rs in Caenorhabditis elegans Research for the Identification of Health-Promoting Natural Products.","authors":"Martina Redl, Anusha Shayegan, Judith M Rollinger","doi":"10.1055/a-2254-0131","DOIUrl":"10.1055/a-2254-0131","url":null,"abstract":"The average age of the population is increasing worldwide, which has a profound impact on our society. This leads to an increasing demand for medicines and requires the development of new strategies to promote health during the additional years. In the search for resources and therapeutics for improved health during an extended life span, attention has to be paid to environmental exposure and ecosystem burdens that inevitably emerge with the extended consumption of medicines and drug development, even in the preclinical stage. The hereby introduced sustainable strategy for drug discovery is built on 3Rs, \"R: obustness, R: eliability, and saving R: esources\", inspired by both the 3Rs used in animal experiments and environmental protection, and centers on the usefulness and the variety of the small model organism Caenorhabditis elegans for detecting health-promoting natural products. A workflow encompassing a multilevel screening approach is presented to maximize the amount of information on health-promoting samples, while considering the 3Rs. A detailed, methodology- and praxis-oriented compilation and discussion of proposed C. elegans health span assays and more disease-specific assays are presented to offer guidance for scientists intending to work with C. elegans, thus facilitating the initial steps towards the integration of C. elegans assays in their laboratories.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":"90 7-08","pages":"576-587"},"PeriodicalIF":2.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141284463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural Characterization, and Antioxidative and Anti-inflammatory Activities of Phylloxanthobilins in Tropaeolum majus, a Plant with Relevance in Phytomedicine. 一种在植物药领域具有重要意义的植物--大叶女贞（Tropaeolum majus）中叶黄素的结构特征、抗氧化和抗炎活性。

IF 2.7 4区医学

Planta medica Pub Date : 2024-06-01 Epub Date: 2024-06-06 DOI: 10.1055/a-2225-8314

Patricia Frei, Christian Nadegger, Angelika M Vollmar, Thomas Müller, Simone Moser

{"title":"Structural Characterization, and Antioxidative and Anti-inflammatory Activities of Phylloxanthobilins in Tropaeolum majus, a Plant with Relevance in Phytomedicine.","authors":"Patricia Frei, Christian Nadegger, Angelika M Vollmar, Thomas Müller, Simone Moser","doi":"10.1055/a-2225-8314","DOIUrl":"10.1055/a-2225-8314","url":null,"abstract":"Tropaeolum majus (garden nasturtium) is a plant with relevance in phytomedicine, appreciated not only for its pharmaceutical activities, but also for its beautiful leaves and flowers. Here, we investigated the phytochemical composition of senescent nasturtium leaves. Indeed, we identified yellow chlorophyll catabolites, also termed phylloxanthobilins, which we show to contribute to the bright yellow color of the leaves in the autumn season. Moreover, we isolated and characterized the phylloxanthobilins from T. majus, and report the identification of a pyro-phylloxanthobilin, so far only accessible by chemical synthesis. We show that the phylloxanthobilins contribute to bioactivities of T. majus by displaying strong anti-oxidative effects in vitro and in cellulo, and anti-inflammatory effects as assessed by COX-1 and COX-2 enzyme inhibition, similar to other bioactive ingredients of T. majus, isoquercitrin, and chlorogenic acid. Hence, phylloxanthobilins could play a role in the efficacy of T. majus in the treatment of urinary tract infections, an established indication of T. majus. With the results shown in this study, we aid in the completion of the phytochemical profile of T. majus by identifying additional bioactive natural products as relevant components of this medicinal plant.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":"90 7-08","pages":"641-650"},"PeriodicalIF":2.7,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156495/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141284471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Phylogenetically Diverse Bacterial Endophytes of Bergenia pacumbis on Bergenin Production in the Plant Cell Suspension Cultures. 荚蒾的系统发育多样性细菌内生菌对植物细胞悬浮培养液中荚蒾苷产量的影响

IF 2.1 4区医学

Planta medica Pub Date : 2024-06-01 Epub Date: 2023-09-06 DOI: 10.1055/a-2162-4018

Christoph Wawrosch, Martina Oberhofer, Stefan Steinbrecher, Sergey B Zotchev

{"title":"Impact of Phylogenetically Diverse Bacterial Endophytes of Bergenia pacumbis on Bergenin Production in the Plant Cell Suspension Cultures.","authors":"Christoph Wawrosch, Martina Oberhofer, Stefan Steinbrecher, Sergey B Zotchev","doi":"10.1055/a-2162-4018","DOIUrl":"10.1055/a-2162-4018","url":null,"abstract":"Plant in vitro cultures are potential sources for secondary metabolites. However, low productivity is often a major drawback for industrial application. Elicitation is an important strategy to improve product formation in vitro. In this context, endophytes are of special interest as biotic elicitors due to their possible interaction with the metabolism of the host plant. A total of 128 bacterial endophytes were isolated from the medicinal plant Bergenia pacumbis and taxonomically classified using 16S rRNA gene sequencing. Five strains belonging to different genera were grown in lysogeny broth and tryptic soy broth medium and cells as well as spent media were used as elicitors in cell suspension cultures of B. pacumbis. Production of the main bioactive compound bergenin was enhanced 3-fold (964 µg/g) after treatment with cells of Moraxella sp. or spent tryptic soy broth medium of Micrococcus sp. These results indicate that elicitation of plant cell suspension cultures with endophytic bacteria is a promising strategy for enhancing the production of desired plant metabolites.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"651-657"},"PeriodicalIF":2.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10167459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0