Planta medica最新文献_第8页

New Constituents from Zanthoxylum rhoifolium. Zanthoxylum rhoifolium Lam.的新成分

IF 2.1 4区医学

Planta medica Pub Date : 2025-03-01 Epub Date: 2024-11-25 DOI: 10.1055/a-2486-9139

Mauro Di Stasi, Valentina Parisi, Vanessa Hernandez, Erica Gazzillo, Maria Giovanna Chini, Giuseppe Bifulco, Alessandra Braca, Giuliana Donadio, Nunziatina De Tommasi

引用次数: 0

Plants with Hair Growth Activity for Alopecia: A Scoping Review on Methodological Aspects. 具有毛发生长活性的植物治疗脱发：方法学方面的综述。

IF 2.1 4区医学

Planta medica Pub Date : 2025-03-01 Epub Date: 2024-12-02 DOI: 10.1055/a-2494-9020

Sharize Betoni Galende, Mariana Nascimento de Paula, Mariana Millan Fachi, Daniela Cristina de Medeiros Araújo, Danielly Chierrito, João Carlos Palazzo de Mello

{"title":"Plants with Hair Growth Activity for Alopecia: A Scoping Review on Methodological Aspects.","authors":"Sharize Betoni Galende, Mariana Nascimento de Paula, Mariana Millan Fachi, Daniela Cristina de Medeiros Araújo, Danielly Chierrito, João Carlos Palazzo de Mello","doi":"10.1055/a-2494-9020","DOIUrl":"10.1055/a-2494-9020","url":null,"abstract":"Alopecia is a common dermatological disorder of patchy hair loss with substantial patient burden. Phytotherapeutic compounds are increasingly used as a source of new therapeutic options. This review aimed to synthesize the evidence on plant species in hair growth and the methodological aspects of in vivo experimental models. The systematic scoping review was conducted following the PRISMA checklist, the Joanna Briggs Institute, and in accordance with Cochrane. A systematic search was carried out in the Pubmed, Scopus, Web of Science, and SciELO databases. In vivo experiments that evaluated hair growth activity using natural substances of plant origin were included. Data collection and analysis: a total of 1250 studies were identified, of which 175 were included for qualitative synthesis. Of these, 128 used mice, 37 rats, 10 rabbits, 1 guinea pig, and 1 sheep as animal models. The methodologies mapped were as follows: hair growth analysis, histological analysis, immunohistochemistry, gene expression analysis, Western blot, enzyme-linked immunosorbent assay, and biochemical analysis. Minoxidil and finasteride were the most commonly used positive controls. The studies evaluated plant species (166), algae (11), or isolated substances (31). Overall, 152 plant species and 37 isolated substances were identified. This is the first systematic scoping review on the methodological aspects of in vivo hair growth activity. We created a checklist to be completed by authors to allow data comparison and reproducibility, facilitate data interpretation by readers, and ensure better quality of evidence. This work may become a valuable tool for future research and contribute to significant advances in hair growth studies.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"89-108"},"PeriodicalIF":2.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142771390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive Biotechnological Strategies for Podophyllotoxin Production from Plant and Microbial Sources. 利用植物和微生物资源生产鬼臼毒素的综合生物技术战略。

IF 2.1 4区医学

Planta medica Pub Date : 2025-03-01 Epub Date: 2024-12-17 DOI: 10.1055/a-2504-3069

Anviksha Anviksha, Mondem Sudhakara Reddy

{"title":"Comprehensive Biotechnological Strategies for Podophyllotoxin Production from Plant and Microbial Sources.","authors":"Anviksha Anviksha, Mondem Sudhakara Reddy","doi":"10.1055/a-2504-3069","DOIUrl":"10.1055/a-2504-3069","url":null,"abstract":"Podophyllotoxin is derived from plant sources and exhibits strong anticancer activity. However, limited natural availability and environmental impacts from traditional extraction methods drive the search for alternative production approaches. This review explores diverse strategies for sustainable podophyllotoxin synthesis, including biosynthesis, semi-synthesis, and biotransformation. Biosynthetic methods involve metabolic pathway engineering in plant or microbial cells, enabling increased yields by manipulating precursor availability and gene expression. Semi-synthetic approaches modify podophyllotoxin precursors or intermediates to enhance therapeutic effects, with derivatives like etoposide and teniposide showing clinical efficacy. Biotransformation, utilising organisms such as endophytic fungi or human hepatic enzymes, enables the transformation of substrates like deoxypodophyllotoxin into podophyllotoxin or its derivatives, yielding compounds with reduced environmental impact and improved purity. The anticancer efficacy of podophyllotoxin and its derivatives stems from multiple mechanisms. These compounds disrupt cell mitosis by inhibiting microtubule assembly, impairing nucleoside transport, and blocking topoisomerase II activity, leading to DNA cleavage and cancer cell apoptosis. Podophyllotoxin and its derivatives also exhibit anti-angiogenesis and anti-metastatic effects through signalling pathway modulation. Notably, derivatives like deoxypodophyllotoxin utilise advanced delivery systems, enhancing targeted efficacy and reducing side effects. Given the varied mechanisms and growing therapeutic applications, optimising biotransformation and delivery techniques remains essential for advancing podophyllotoxin-based therapies. This comprehensive review underscores the compound's potential as a robust anticancer agent and the need for continued research to maximise its production and clinical effectiveness.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"66-88"},"PeriodicalIF":2.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracts of Drynariae Rhizoma Promote Bone Formation in OVX Rats through Modulating the Gut Microbiota. 旱莲草提取物通过调节肠道微生物群促进卵巢切除大鼠的骨形成

IF 2.1 4区医学

Planta medica Pub Date : 2025-03-01 Epub Date: 2024-11-05 DOI: 10.1055/a-2462-4844

Qing Lin, Xinchen Ouyang, Qi Pan, Jiajia Huang, Zhifen Zhang, Yumei Yang, Haoyu Wang, Li Yang, Xiaofeng Zhu, Xiaoyun Li, Ronghua Zhang

{"title":"Extracts of Drynariae Rhizoma Promote Bone Formation in OVX Rats through Modulating the Gut Microbiota.","authors":"Qing Lin, Xinchen Ouyang, Qi Pan, Jiajia Huang, Zhifen Zhang, Yumei Yang, Haoyu Wang, Li Yang, Xiaofeng Zhu, Xiaoyun Li, Ronghua Zhang","doi":"10.1055/a-2462-4844","DOIUrl":"10.1055/a-2462-4844","url":null,"abstract":"Drynariae Rhizoma has been commonly used as a preventive and therapeutic agent for bone diseases. However, its pharmacological mechanisms have not been fully elucidated. Here, we aimed to investigate the effects of Drynariae Rhizoma in a bilateral ovariectomized rat model and explore the correlation with gut microbiome. We established an ovariectomized rat model, which we treated with different doses of Drynariae Rhizoma (Drynariae Rhizoma-Low, 0.27 g/kg/day; Drynariae Rhizoma-Middle, 0.81 g/kg/day; Drynariae Rhizoma-High, 2.43 g/kg/day) through intragastric administration for 12 weeks. Results showed that Drynariae Rhizoma alleviated body weight, moderated bone microstructure, and promoted the expression of bone formation-related factors in ovariectomized rats, in which Drynariae Rhizoma-High showed the most significant effects among the three doses. Furthermore, the effects of Drynariae Rhizoma on promoting bone formation were correlated to the changes in microbial richness and the restorations of several genera, among which Ruminiclostridium and Ruminococcaceae_UCG_007 were positively correlated with the bone formation-related factors, and both were enriched in the Drynariae Rhizoma-High group as biomarkers. Moreover, CMP-legionaminate biosynthesis I might be a crucial pathway of Drynariae Rhizoma to regulate gut microbiota. The content of serum short-chain fatty acids in the ovariectomized rats were regulated by Drynariae Rhizoma. Our results demonstrate that Drynariae Rhizoma promotes bone formation in ovariectomized rats, and is related to the regulation of the gut microbiota structure.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"127-141"},"PeriodicalIF":2.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11928296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aloe vera and the Proliferative Phase of Cutaneous Wound Healing: Status Quo Report on Active Principles, Mechanisms, and Applications. 芦荟与皮肤伤口愈合的增殖阶段：活性原理、机制和应用现状报告》。

IF 2.1 4区医学

Planta medica Pub Date : 2025-01-01 Epub Date: 2024-11-20 DOI: 10.1055/a-2446-2146

Zhuang Min Lee, Bey Hing Goh, Kooi Yeong Khaw

{"title":"Aloe vera and the Proliferative Phase of Cutaneous Wound Healing: Status Quo Report on Active Principles, Mechanisms, and Applications.","authors":"Zhuang Min Lee, Bey Hing Goh, Kooi Yeong Khaw","doi":"10.1055/a-2446-2146","DOIUrl":"10.1055/a-2446-2146","url":null,"abstract":"Aloe vera is commonly used as traditional medicine for cutaneous wound healing. Nonetheless, the wound healing mechanisms of Aloe vera remain unclear. This review aims to provide insight into the molecular mechanisms of Aloe vera in promoting cutaneous wound healing, with particular emphasis on the mechanisms that stimulate cell proliferation and migration. Aloe vera has been shown to upregulate growth factors such as keratinocyte growth factor-1 (KGF-1), transforming growth factor-β (TGF-β), cyclin D1, insulin-like growth factor 1 (IGF-1), vascular endothelial growth factor (VEGF), basic fibroblastic growth factor (bFGF), and microfibril-associated glycoprotein 4 (MFAP4), as well as collagen, fibrillin, elastin, α-smooth muscle actin (α-SMA), integrins, and platelet endothelial cell adhesion molecule 1 (PECAM-1, also known as CD31), while downregulating the expression of matrix metalloproteinases (MMPs). In addition, Aloe vera was also found to upregulate PI3K/Akt and MAPK pathways, as well as the TGF-β signalling pathway via Smad proteins. Furthermore, molecular docking studies revealed that certain chemical constituents of Aloe vera bind to some of the forementioned growth factors or signalling molecules. With regards to current applications, although human clinical trials have reported positive results from using Aloe vera in healing open wounds and burns and alleviating some inflammatory skin diseases, the current commercial uses of Aloe vera remain largely focused on cosmetic products. Thus, greater advances are required to promote the use of Aloe vera products in clinical settings.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"4-18"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-Inflammatory Activity of Labdane and Norlabdane Diterpenoids from Leonurus sibiricus Related to Modulation of MAPKs Signaling Pathway. 西比露中的拉布丹和诺拉布丹二萜类化合物的抗炎活性与调节 MAPKs 信号通路有关

IF 2.1 4区医学

Planta medica Pub Date : 2025-01-01 Epub Date: 2024-10-12 DOI: 10.1055/a-2440-5166

Nguyen Minh Trang, Le Ba Vinh, Nguyen Viet Phong, Seo Young Yang

{"title":"Anti-Inflammatory Activity of Labdane and Norlabdane Diterpenoids from Leonurus sibiricus Related to Modulation of MAPKs Signaling Pathway.","authors":"Nguyen Minh Trang, Le Ba Vinh, Nguyen Viet Phong, Seo Young Yang","doi":"10.1055/a-2440-5166","DOIUrl":"10.1055/a-2440-5166","url":null,"abstract":"Leonurus sibiricus, a widely cultivated herbaceous plant in Asian countries, exhibits diverse medicinal applications. Recent studies emphasize its pharmacological properties and efficacy in promoting bone health. In addition to the known compounds and their pharmacological activities, in this study, we isolated and elucidated two new labdane-type diterpenoids, (3R,5R,6S,10S)-3,6-dihydroxy-15-ethoxy-7-oxolabden-8(9),13(14)-dien-15,16-olide (1: ) and (4R,5R,10S)-18-hydroxy-14,15-bisnorlabda-8-en-7,13-dione (2: ), a new natural phenolic compound, and a known compound from L. sibiricus using advanced spectroscopic techniques, including circular dichroism spectroscopy, high-resolution mass spectrometry, and 1- and 2-dimensional NMR. Among these, compound 1: demonstrated potent inhibition of nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) mRNA expression levels, followed by compound 2: . Whereas compounds 3: and 4: did not exhibit effectiveness in RAW264.7 macrophages. Moreover, compound 1: suppressed pro-inflammatory markers induced by lipopolysaccharide (LPS) stimulation. Compound 1: also suppressed iNOS and cyclooxygenase-2 (COX-2) protein levels and downregulated pro-inflammatory cytokines. Additionally, compound 1: showed inhibition of the phosphorylation of p38, JNK, and ERK, key mediators of the MAPK signaling pathway. These findings indicate that a natural-derived product, compound 1,: might be a potential candidate as an anti-inflammation mediator.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"29-39"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Turkish Astragalus Species: Botanical Aspects, Secondary Metabolites, and Biotransformation. 土耳其黄芪品种：植物学方面、次级代谢物和生物转化。

IF 2.1 4区医学

Planta medica Pub Date : 2025-01-01 Epub Date: 2024-11-13 DOI: 10.1055/a-2444-3252

Güner Ekiz Dinçman, Zeki Aytaç, İhsan Çalış

{"title":"Turkish Astragalus Species: Botanical Aspects, Secondary Metabolites, and Biotransformation.","authors":"Güner Ekiz Dinçman, Zeki Aytaç, İhsan Çalış","doi":"10.1055/a-2444-3252","DOIUrl":"10.1055/a-2444-3252","url":null,"abstract":"Astragalus is a widespread genus comprising approximately 3500 species, both annual and perennial, found across Asia, Europe, Africa, and the Americas. In Turkey, it is represented by 63 sections and 485 taxa with a high endemism ratio (51%). In traditional medicine, the roots of various Astragalus species represent very old and well-known drugs used for antiperspirant, diuretic, and tonic purposes, as well as for the treatment of nephritis, diabetes, leukemia, and uterine cancer. The genus Astragalus is the richest source of cycloartane-type compounds, which display a diverse range of bioactivities, such as wound healing, immunomodulatory, antitumor, hepatoprotective, antimutagenic, antiviral, and antiprotozoal activities. Moreover, cycloastragenol, the main sapogenol of many cycloartane-type glycosides found in the Astragalus genus, has gained attention as a potent telomerase activator over the past decade. The preparation of cycloastragenol derivatives could be significant in the near future due to their unique bioactivity. This review covers the botanical aspects of Astragalus L., as well as the phytochemical and biological activity studies conducted on Turkish Astragalus species, with a special focus on cycloartenols. It contains 36 articles reporting the phytochemistry of 29 Astragalus species and 111 new compounds, including 104 triterpene saponins. In addition to the phytochemical studies, this review summarizes the biotransformation studies on Astragalus cycloartanes via endophytic fungi isolated from the tissues of Astragalus species.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"40-61"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Betulinic Acid Acts in Synergism with Imatinib Mesylate, Triggering Apoptosis in MDR Leukemia Cells. 白桦脂酸与甲磺酸伊马替尼协同作用，引发 mdr 白血病细胞凋亡。

IF 2.1 4区医学

Planta medica Pub Date : 2025-01-01 Epub Date: 2024-10-12 DOI: 10.1055/a-2440-4847

Claudia Stutz, Ana Paula Gregório Alves Fontão, Gustavo Werneck de Souza E Silva, Leonardo Noboru Seito, Renata Trentin Perdomo, André Luiz Franco Sampaio

{"title":"Betulinic Acid Acts in Synergism with Imatinib Mesylate, Triggering Apoptosis in MDR Leukemia Cells.","authors":"Claudia Stutz, Ana Paula Gregório Alves Fontão, Gustavo Werneck de Souza E Silva, Leonardo Noboru Seito, Renata Trentin Perdomo, André Luiz Franco Sampaio","doi":"10.1055/a-2440-4847","DOIUrl":"10.1055/a-2440-4847","url":null,"abstract":"Chronic myeloid leukemia (CML) is a myeloproliferative disease, characterized by the presence of the oncogene BCR-ABL. Imatinib mesylate (IMA) is the first-line treatment for CML, and some treatment resistance has been reported. Natural products are rich sources of bioactive compounds with biological effects, opening a possibility to alter cell susceptibility to drugs such as imatinib. Herein, we evaluated the interference of betulinic acid and ursolic acid in glycoprotein P (P-gp) activity and the possible synergistic effect when associated with IMA by the Chou-Talalay method. Ursolic acid presented an IC50 of 14.0 µM and 19.6 µM for K562 and Lucena 1, respectively, whilst betulinic acid presented an IC50 of 8.6 µM and 12.5 µM for these cell lines. Evaluation of the combination of terpenoids and imatinib mesylate revealed that ursolic acid or betulinic acid acts in synergism with IMA, as indicated by the combination indexes (CI<1). Analysis of annexin V labeling demonstrated that a combination of IMA with betulinic acid enhances the inhibition on cell proliferation via the apoptosis pathway, with caspases 3/7 activation after 24 hours of treatment and inhibition of the STAT5/survivin pathway, decreasing cell viability. The combination of natural products and IMA on a multidrug-resistant leukemia cell line is a promising strategy for CML treatment.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"19-28"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Medicinal Plant Microbiomes: Factors Affecting Bacterial and Fungal Community Composition. 药用植物微生物群：影响细菌和真菌群落组成的因素

IF 2.1 4区医学

Planta medica Pub Date : 2024-12-01 Epub Date: 2024-10-24 DOI: 10.1055/a-2420-0270

Daniel Zagal, James G Graham, Jonathan Bisson, Stefan J Green, Guido F Pauli

{"title":"Medicinal Plant Microbiomes: Factors Affecting Bacterial and Fungal Community Composition.","authors":"Daniel Zagal, James G Graham, Jonathan Bisson, Stefan J Green, Guido F Pauli","doi":"10.1055/a-2420-0270","DOIUrl":"10.1055/a-2420-0270","url":null,"abstract":"This exploratory study was designed to identify factors implicating microbial influence on medicinal plant metabolomes. Utilizing a whole-microbiome approach, amplicon sequencing was used to identify the makeup of fungal and bacterial assemblages from endophytic (interior) and epiphytic (external) environments in two different sets of congeneric host-plant pairs, with collection of multiple samples of two medicinal plant species (Actaea racemosa, Rhodiola rosea) and two generic analogs (Actaea rubra, Rhodiola integrifolia). Diversity analysis of microbial assemblages revealed the influence of three primary factors driving variance in microbial community composition: host-plant taxonomy, the compartmentalization of microbial communities within discrete plant parts, and the scale of distance (microhabitat heterogeneity) between sampling locations. These three factors accounted for ~ 60% of variance within and between investigated microbiomes. Across all our collections, bacterial populations were more diverse than fungi (per compartment), and microbial density in epiphytic compartments (aerial parts, rhizosphere) were higher than those of endophytes (leaf and root). These comparative data point to key loci associated with variation between congeneric pairs and plant genera, providing insight into the complex and contrasting relationships found within this multi-kingdom coevolutionary relationship. Although reflective of only a limited set of botanical source materials, these data document the richness of a relatively unexplored component of the plant world and highlight the relevance of a whole-microbiome ecology-driven approach to botanical research and directed natural product investigations.","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"1130-1142"},"PeriodicalIF":2.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11816503/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142505901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Echinacea purpurea Extracts as Immunostimulants: Impact on Macrophage Activation. 紫锥菊提取物作为免疫刺激剂的评估：对巨噬细胞活化的影响

IF 2.1 4区医学

Planta medica Pub Date : 2024-12-01 Epub Date: 2024-10-17 DOI: 10.1055/a-2436-9664

Sara F Vieira, Samuel M Gonçalves, Virgínia M F Gonçalves, Maria E Tiritan, Cristina Cunha, Agostinho Carvalho, Rui L Reis, Helena Ferreira, Nuno M Neves

{"title":"Evaluation of Echinacea purpurea Extracts as Immunostimulants: Impact on Macrophage Activation.","authors":"Sara F Vieira, Samuel M Gonçalves, Virgínia M F Gonçalves, Maria E Tiritan, Cristina Cunha, Agostinho Carvalho, Rui L Reis, Helena Ferreira, Nuno M Neves","doi":"10.1055/a-2436-9664","DOIUrl":"10.1055/a-2436-9664","url":null,"abstract":"Echinacea purpurea has been traditionally used to strengthen the immune system. Therefore, herein, we investigated the potential of E. purpurea aqueous extracts (AEs) obtained from flowers (F), leaves (L), or roots (R) as an immune booster in human primary monocyte-derived macrophages (hMDMs). Additionally, to identify the main class of compounds (phenolic/carboxylic acids vs. alkylamides) responsible for the bioactivity, the three AEs were fractioned by semi-preparative high-performance liquid chromatography (HPLC). The AEs and the isolated phenolic/carboxylic acidic fractions were not cytotoxic for hMDMs for all tested concentrations, as confirmed by the metabolic activity and DNA content assays. Moreover, AE drastically induced the production of the interleukin (IL)-6 and tumor necrosis factor (TNF)-α, with a minimal effect on IL-1β and prostaglandin E2 (PGE2), supporting their potential for macrophage activation. Interestingly, in the presence of the phenolic/carboxylic acidic fractions, this efficacy considerably decreased, suggesting a complementary effect between compounds. AE also triggered the phosphorylation of the extracellular signal-regulated kinase (ERK) 1/2 and p38 signaling pathways and upregulated the cyclooxygenase (COX)-2 expression in hMDMs. Overall, AE-F was demonstrated to be the most powerful immunostimulant extract that can be related to their higher number in identified bioactive compounds compared to AE-L and AE-R. These results highlight the efficiency of E. purpurea AE to enhance the function of a key cell type of the immune system and their potential as immunostimulant formulations for patients with a compromised immune system due to certain diseases (e.g., acquired immunodeficiencies) and treatments (e.g., chemotherapy).","PeriodicalId":20127,"journal":{"name":"Planta medica","volume":" ","pages":"1143-1155"},"PeriodicalIF":2.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0