New constituents from Zanthoxylum rhoifolium Lam.

Abstract

The phytochemical study of Zanthoxylum rhoifolium apolar and medium polarity stem bark and leaves extracts afforded twenty-nine compounds, including three new sesquiterpenes (1: -3: ) and one new α-ionone glycoside (4: ). All compounds were characterized by means of 1D and 2D NMR and HRESIMS data. Furthermore, a precise structural analysis was performed, employing a combined density functional theory (DFT)/NMR approach to elucidate the compounds configuration. The crude extracts were then tested against a panel of Gram-positive and Gram-negative bacteria, by broth dilution methods to determine their minimal inhibitory concentration (MIC); in these experimental conditions no interesting antimicrobial activity was observed.