Nitidine Isolated from the Bark of Zanthoxylum myriacanthum and its Effects on NTERA-2 Cancer Stem Cells.

IF 2 4区医学 Q3 CHEMISTRY, MEDICINAL

Planta medica Pub Date : 2025-10-01 Epub Date: 2025-07-23 DOI:10.1055/a-2654-6627

Trieu Ha Phuong, Pham Minh Quan, Pham Cao Bach, Tran Thi Tuyen, Nguyen Phuong Nga, Nguyen Thi Cuc, Do Thi Thao, Nguyen Thi Hong Van

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引用次数: 0

Abstract

Nitidine (NIT) was isolated from the bark of Zanthoxylum myriacanthum and assessed for anti-proliferative effects on NTERA-2 cancer stem cells using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay, spheroid assay, DNA and lysosome staining, flow cytometry, caspase assay, immunoblotting, and molecular docking studies. Moreover, nitidine suppresses stemness properties like in vitro tumorsphere forming, c-myc, Oct4, Nanog proteins of NTERA-2 cancer stem cells after 48-hour treatment. Nitidine selectively induced anti-survival activities by triggering the intrinsic apoptotic process through p53 signaling and lysosome-dependent cell death (LDCD). The mechanism of action of nitidine on cancer stem cells was also investigated using molecular docking studies to provide physical insights. Molecular docking studies revealed that nitidine induces LDCD by effectively inhibiting the MHR1/2 domain of the TRPM2 protein on liposome membrane. These results suggested the potential capacity of nitidine in inhibiting cancer stem cells or tumor-initiating cells for therapeutic cancer application.

查看原文本刊更多论文

多棘花椒皮中尼替丁的分离及其对肿瘤干细胞的作用。

采用3-[4,5-二甲基噻唑-2-基]-2,5二苯基溴化四唑试验、球体试验、DNA和溶酶体染色、流式细胞术、caspase试验、免疫印迹和分子对接研究，对从花椒皮中分离得到的Nitidine （NIT）对NTERA-2癌干细胞的抗增殖作用进行了评估。此外，尼替丁在48小时后抑制NTERA-2肿瘤干细胞的干细胞特性，如体外肿瘤球形成、c-myc、Oct4、Nanog蛋白。尼替丁通过p53信号传导和溶酶体依赖性细胞死亡（LDCD）触发内在凋亡过程，选择性地诱导抗存活活性。通过分子对接研究，研究了尼替丁对肿瘤干细胞的作用机制。分子对接研究表明，尼替丁通过有效抑制脂质体膜上TRPM2蛋白的MHR1/2结构域诱导LDCD。这些结果表明，尼替丁在抑制肿瘤干细胞或肿瘤起始细胞治疗癌症方面具有潜在的能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Planta medica 医学-药学

CiteScore

5.10

自引率

3.70%

发文量

101

审稿时长

1.8 months

期刊介绍： Planta Medica is one of the leading international journals in the field of natural products – including marine organisms, fungi as well as micro-organisms – and medicinal plants. Planta Medica accepts original research papers, reviews, minireviews and perspectives from researchers worldwide. The journal publishes 18 issues per year. The following areas of medicinal plants and natural product research are covered: -Biological and Pharmacological Activities -Natural Product Chemistry & Analytical Studies -Pharmacokinetic Investigations -Formulation and Delivery Systems of Natural Products. The journal explicitly encourages the submission of chemically characterized extracts.