Phytochemical Analysis最新文献

{"title":"Advances in Extraction and Quantification of Minor Phytocannabinoids.","authors":"Nikita Gandlevskiy, Alessandro Barge, Giancarlo Cravotto","doi":"10.1002/pca.70040","DOIUrl":"10.1002/pca.70040","url":null,"abstract":"<p><p>Ever since the chemical structures of major phytocannabinoids, such as Δ⁹-tetrahydrocannabinol (THC) and cannabidiol (CBD), were elucidated, the majority of research has focused primarily on these compounds, often overlooking the other ~160 minor cannabinoids identified in Cannabis sativa to date. However, in recent years, these previously understudied cannabinoids have garnered increasing scientific attention due to advancements in highly sensitive analytical techniques that enable their detection in plant matrices. Moreover, early-stage clinical trials have demonstrated that several minor cannabinoids exhibit promising therapeutic potential. This review aims to provide a comprehensive summary of recent developments in the extraction, analysis, and potential applications of selected minor phyt cannabinoids, with the goal of facilitating future research in this field. A thorough analysis of the latest data has been conducted to offer a detailed overview of current extraction and quantification methodologies for minor cannabinoids.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"156-181"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145649106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Insights Into Curcumin Behavior After Its Biosynthesis in Engineered Escherichia coli Through Spectroscopic and Spatial Analysis.","authors":"Hassan Sher, Hui Wang, Hayat Ullah, T T Trinh Phan, Jesse B Brown, M Tuan Trinh, Yi Rao, Jixun Zhan","doi":"10.1002/pca.70041","DOIUrl":"10.1002/pca.70041","url":null,"abstract":"<p><strong>Introduction: </strong>Curcumin is a naturally occurring compound with anti-inflammatory, cholesterol-lowering, antidiabetic, and antioxidant properties. Curcumin can be biosynthesized in microbial hosts despite being found natively in Curcuma longa roots.</p><p><strong>Objectives: </strong>It is unclear whether curcumin is transported out of microbial cells, binds to the inner or outer cell membrane, or accumulates inside. This study aims to gain a better understanding of curcumin's behavior after its formation in the host, which could lead to the development of new approaches to improve curcumin production.</p><p><strong>Material and methods: </strong>Advanced imaging techniques, including SEM, TEM, SHS, and TPF, were utilized to understand the behavior of curcumin within and outside of engineered Escherichia coli cells.</p><p><strong>Results: </strong>Curcumin was biosynthesized from ferulic acid in engineered E. coli BL21(DE3) by coexpressing 4-coumarate: CoA ligase and curcuminoid synthase. Second harmonic scattering (SHS) spectroscopy experiments utilized curcumin as a probe to investigate the surface binding of curcumin onto living E. coli cells with experimentally determined adsorption free energy, ΔG, values of -14.0 kcal/mol. By employing second harmonic and two-photon fluorescence imaging methods, the spatial distribution of curcumin aggregates was determined. Electron microscopy images revealed the presence of curcumin aggregates within the cells, at the surface, and in the media.</p><p><strong>Conclusion: </strong>These experiments demonstrate that curcumin biosynthesis from ferulic acid leads to significant product aggregation within the cells, which could ultimately halt production by inducing cell death. Understanding the localization, transport, and removal of curcumin is crucial in developing more efficient biosynthetic pathways to enhance its production in microbial systems.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"321-329"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145649096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sesquiterpenoids and Polyacetylenes From Notopterygium Incisum With Anti-Rheumatoid Arthritis Activity.","authors":"Fan Wu, Wen-Jian Gu, Lu Chen, Bing Xia, Yu Wang, Li-Jun Huang, Ke Wang, Yu-Cheng Gu, Yan Zhou","doi":"10.1002/pca.70037","DOIUrl":"10.1002/pca.70037","url":null,"abstract":"<p><strong>Introduction: </strong>Notopterygium incisum is a traditional Chinese and Tibetan medicinal herb widely used for treating colds, rheumatism, and musculoskeletal disorders.</p><p><strong>Objectives: </strong>The aims of the study were to isolate sesquiterpenoids and polyacetylenes from N. incisum rhizomes and to evaluate their potential anti-rheumatoid arthritis activity.</p><p><strong>Methods: </strong>Compounds were isolated using chromatographic techniques. Structures were elucidated by 1D/2D NMR, UV, IR, and HRESIMS, and absolute configurations were assigned using TD-DFT ECD calculations. Their anti-RA activity was assessed by measuring pro-inflammatory cytokine inhibition in TNF-α-induced MH7A cells. Some compounds were evaluated by molecular docking and molecular dynamics simulations to further explore possible molecular targets related to RA.</p><p><strong>Results: </strong>Twenty-one compounds, including seven new ones and one newly identified natural product, were obtained. Several compounds showed significant inhibition of pro-inflammatory cytokines in TNF-α-induced MH7A cells. Molecular docking also revealed varying degrees of binding affinity to RA-relevant proteins, and dynamics simulations confirmed the stability of most selected complexes.</p><p><strong>Conclusions: </strong>A series of sesquiterpenoids and polyacetylenes was isolated. Several compounds exhibited notable anti-inflammatory activity, suggesting that these structural types may underlie the anti-RA effects of N. incisum.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"273-286"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145471735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discrimination of Three Panax Plants Based on Small Molecular Weight Saccharide Fractions Using HPAEC-PAD Coupled With Multivariate Statistical Models.","authors":"Yue Wang, Xue-Qing Liu, Jun Liang, Hai-Xue Kuang, Yong-Gang Xia","doi":"10.1002/pca.70035","DOIUrl":"10.1002/pca.70035","url":null,"abstract":"<p><strong>Introduction: </strong>Accurate discrimination of Panax ginseng, P. quinquefolius, and P. notoginseng is crucial in traditional Chinese medicine (TCM). Distinct from conventional small molecule compounds (e.g., saponins, volatile oils), proteins, or polysaccharides, this study pioneers small molecular weight saccharide fractions (SMS) as a novel class of biomarkers for species differentiation.</p><p><strong>Objectives: </strong>This study aimed to develop an SMS-based method for distinguishing the three Panax plants.</p><p><strong>Methods: </strong>A simple and reproducible workflow was developed for obtaining natural SMS from the three Panax species through systematic optimization of ultrasonic extraction protocols and purification procedures. The chemical structures of three SMS were characterized by integrating non-targeted HILIC-ESI^--QTOF-MS^E with targeted HILIC-ESI^--QTOF-MS² analysis. SMS-based fingerprinting of the three Panax species was constructed using HPAEC-PAD, followed by multivariate statistical models (PCA, PLS-DA, and LDA models) for species discrimination and cluster analysis.</p><p><strong>Results: </strong>The optimal extraction conditions were identified as 60°C, a solid-liquid ratio of 1:30, 50 min duration, and 50% ethanol concentration. SMS with degrees of polymerization (DPs) 2 to 13 were identified, revealing preliminary structural differences among the three Panax species. The established HPAEC-PAD fingerprinting combined with multivariate models (PCA, PLS-DA, and LDA) enabled distinct species separation and achieved high classification accuracy, with successful prediction of external validation samples.</p><p><strong>Conclusions: </strong>This study presents the first integration of HPAEC-PAD fingerprinting with multivariate statistical models for successfully discriminating three Panax species based on SMS, validating SMS as a reliable class of biomarkers for species identification and offering a distinct alternative to traditional markers.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"228-240"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145459507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dereplicative Combination of HPLC/DAD/MS and 2D NMR to Identify Lichexanthone Isomers in Lichen Extracts.","authors":"Solenn Ferron, Marylène Chollet-Krugler, Hermann Pinson, Rania Marzoug, Philippe Uriac, Françoise Lohézic-Le Dévéhat","doi":"10.1002/pca.70043","DOIUrl":"10.1002/pca.70043","url":null,"abstract":"<p><strong>Introduction: </strong>Lichexanthones are the major xanthones found in lichens. They present a high degree of isomerism, which makes their identification tedious. Xanthones are known to occur in lichens according to chemosyndromes, and these compounds act as chemotaxonomic markers. Many lichens that produce xanthones are crustose lichens from Lecanora or Pertusaria genera, which often leads to small amounts of extracts being analyzed.</p><p><strong>Objective: </strong>We aimed to set up a method able to identify the right isomers of lichexanthones contained in the extract of a xanthone-producing lichen. This method has to produce reliable results even without all the possible isomers at hand, as they are numerous and difficult to obtain.</p><p><strong>Material and methods: </strong>The 16 norlichexanthones were obtained by a strategy combining isolation and synthesis. All of them were characterized by a full set of NMR experiments, highlighting key features, and an HPLC/DAD/MS method was developed. To exemplify the method, selected lichens were submitted to acetone microextraction and the extracts were analyzed by HPLC/DAD/MS and NMR.</p><p><strong>Results: </strong>All norlichexanthones were well separated by HPLC/DAD/MS, which enables their identification in the lichen extracts, provided that the retention time of all the isomers is known; ¹³C NMR is very informative about the position of chlorine in norlichexanthones but lacks sensitivity. 2D NMR provides a high level of structural information even on complex extracts.</p><p><strong>Conclusion: </strong>In addition to HPLC/DAD/MS, NMR can be used directly on a lichen extract to confirm the positions of the chlorine atoms on the lichexanthone scaffold, thanks to the HSQC experiment. Furthermore, the NOESY experiment gives the position of methylations for a comprehensive overview of the substitution patterns involved in the extract of a xanthone-producing lichen, without requiring the entire series of the 64 derivatives.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"330-343"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12961365/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145708956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coaxial Oil-Polymer-Salt Three-Liquid-Phase Centrifugal Platform for the Extraction of the Active Ingredients From the Natural Products.","authors":"Aoli Ye, Yue Xiong, Ranran Ma, Xinyi Yang, Peng-Bo Duan, Anqi Wu, E-Hu Liu, Dong Liu, Deli Xiao","doi":"10.1002/pca.70026","DOIUrl":"10.1002/pca.70026","url":null,"abstract":"<p><strong>Introduction: </strong>Platycladus orientalis (L.) Franco is a unique tree species in China with a long cultivation history and diverse medicinal values. Its dried branches and leaves, called Platycladi Cacumen (PC), contain bioactive compounds. However, conventional extraction methods such as leaching or steam distillation are often associated with high consumption of reagents, time, and energy.</p><p><strong>Objectives: </strong>This study aims to develop an efficient and environmentally friendly approach for extracting flavonoids from PC by integrating the \"oil-polymer-salt\" three-liquid-phase system (OPS-TLPS) with coaxial liquid centrifugal technology.</p><p><strong>Material and methods: </strong>The OPs-TLPS was constructed using di(2-ethylhexyl) phosphate (D2EHPA), polyethylene glycol (PEG), and sodium citrate. During the study, a stable OPs-TLPS was screened by varying the phase-forming substances of the salts and oil-phase reagents, and the effects of three-phase mass fraction and reaction conditions on the extraction of active components from PC were investigated.</p><p><strong>Results: </strong>The D2EHPA-PEG-sodium citrate system demonstrated high stability and efficiency for flavonoid extraction. Key factors, such as D2EHPA concentration, mass fractions of PEG and sodium citrate, pH, and centrifugal time, significantly influenced yield. After 2 h of extraction, over 50% of flavonoids were successfully partitioned into the oil and PEG phases. The integration of OPS-TLPS with coaxial centrifugation notably reduced organic solvent usage and improved extraction efficiency, offering a more automated and greener alternative to conventional techniques for isolating natural products.</p><p><strong>Conclusion: </strong>This method provides a novel, sustainable, and efficient strategy for extracting flavonoids from Platycladi Cacumen, with potential applications in natural product extraction.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"52-71"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperspectral Imaging for Detection and Classification of Plant Primary and Secondary Metabolites: A Review.","authors":"Muskan Raghav, Akhilesh Dubey, Jyotsna Singh","doi":"10.1002/pca.70029","DOIUrl":"10.1002/pca.70029","url":null,"abstract":"<p><strong>Background: </strong>Hyperspectral imaging (HSI) is a nondestructive technique that simultaneously captures spectral and spatial information across multiple wavelengths. It has gained importance in plant science for detecting primary metabolites, vital for growth, and secondary metabolites, essential for plant defense and human health. Conventional methods such as chromatography and mass spectrometry, though accurate, are destructive, time-consuming, and require laborious sample preparation.</p><p><strong>Objectives: </strong>This review examines the potential of HSI as a rapid and noninvasive tool for metabolite detection and classification, emphasizing its role in precision agriculture, plant phenotyping, and medicinal plant research.</p><p><strong>Methods: </strong>This review summarizes principles of HSI, hardware components, image acquisition strategies, and processing techniques. Special focus is given to the integration of machine learning for extracting and classifying biochemical information from high-dimensional spectral data.</p><p><strong>Results: </strong>Studies show that HSI enables accurate, real-time assessment of plant metabolic profiles. Machine learning approaches enhance predictive performance, while advances in imaging sensors, illumination systems, and computational tools are improving applicability. HSI is increasingly adopted for monitoring plant quality, stress responses, and bioactive compound content.</p><p><strong>Conclusion: </strong>This review highlights HSI as a transformative tool in plant metabolomics, providing scalable, rapid, and sustainable alternatives to traditional methods, with strong potential to advance agricultural productivity and medicinal plant applications.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"4-27"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145233118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive Spirocyclic C-Arylglycosides From the Fungal-Fungal Co-Culture of Pestalotiopsis sp. and Nigrospora sp.","authors":"Meifang Wu, Yang Jin, Ying Chen, Ziqian Zeng, Wenbin Deng, Xiangdong Su, Yongbo Xue","doi":"10.1002/pca.70024","DOIUrl":"10.1002/pca.70024","url":null,"abstract":"<p><strong>Introduction: </strong>In this study, two new papulacandin analogs (1-2), together with 12 known compounds (3-14), were identified from the co-culture of two endophytic fungi, Pestalotiopsis sp. J025 and Nigrospora sp. Wn-2-2, which were isolated from Wikstroemia nutans Champ. ex Benth.</p><p><strong>Objectives: </strong>The aim of this study is to investigate the antifungal activity of papulacandins and other secondary metabolites from the co-culture of two endophytic fungi.</p><p><strong>Material and methods: </strong>Their chemical structures, including relative configurations, were elucidated using extensive spectroscopic analyses, including HRESIMS, 1D and 2D NMR, ECD calculations, and quantum mechanical-NMR calculations, as well as comparison with reported analogs.</p><p><strong>Results: </strong>Compound 3 demonstrated promising in vitro antifungal activity against fluconazole-resistant Candida albicans with a minimum inhibitory concentration in 50% isolates (MIC₅₀) of 0.57 ± 0.01 μM.</p><p><strong>Conclusion: </strong>These findings demonstrate that the papulacandin family is a promising resource for the development of antifungal agents against invasive Candida infections.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"28-39"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144964899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polysaccharides as the Key Differentiator in Raw and Processed Pinellia ternata Tubers: Immunostimulatory Effects and Mechanism of Action.","authors":"Trung Huy Ngo, Sunil Mishra, Yun-Seo Kil, Zhiying Chen, Hyukjae Choi, Jae-Hoon Chang, Joo-Won Nam","doi":"10.1002/pca.70032","DOIUrl":"10.1002/pca.70032","url":null,"abstract":"<p><strong>Introduction: </strong>Raw tubers of Pinellia ternata (PTRs) and processed ones (PTPs) have varied therapeutic applications in traditional medicine. PTRs are used to treat cancer, whereas PTPs are used to treat coughing and phlegm. The underlying reason for this difference is still unknown.</p><p><strong>Objectives: </strong>This study aimed to investigate whether polysaccharides are the key differentiators in the traditional uses of PTRs and PTPs and to explore the possible mechanism for the immunomodulatory effects of polysaccharides from PTRs.</p><p><strong>Materials and methods: </strong>PTRs and PTPs were used to produce the total polysaccharides PTR.PS and PTP.PS, respectively, which were physiochemically characterized using various techniques, such as gel permeation chromatography, nuclear magnetic resonance spectroscopy and high-performance liquid chromatography. Their immunomodulatory activity was evaluated by flow cytometry analysis in bone marrow-derived macrophages (BMDMs) and bone marrow-derived dendritic cells (BMDCs) for the analyses of activation markers and concentrations of cytokines secreted. The mechanistic study was elucidated by Western blot analysis.</p><p><strong>Results: </strong>Physicochemical characterization revealed that PTR.PS had lower molecular weights and greater abundance of monosaccharide components, including mannose and galacturonic acid, than PTP.PS. The bioactivity analysis result showed that PTR.PS has robust immunostimulatory effects on BMDMs and BMDCs by the upregulation of NF-κB and mitogen-activated protein kinase (MAPK) pathways leading to the increased expression of activation markers and cytokine secretion. The proinflammatory M1 macrophages induce an inflammatory condition, and the activated dendritic cells can activate T cells for a cell-mediated adaptive immune response.</p><p><strong>Conclusions: </strong>Polysaccharides are the key difference between PTRs and PTPs, resulting in different immunostimulatory effects, which are regulated through NF-κB and MAPK pathways.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"127-139"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145150467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality Consistency Monitoring of Alkaloids of Sophora flavescens by Tandem High-Performance Liquid Chromatography Fingerprinting and Linear Quantitative Profiling Method.","authors":"Zhifei Hou, Yongzhen Chang, Jing Zhang, Yan Li, Guoxiang Sun","doi":"10.1002/pca.70027","DOIUrl":"10.1002/pca.70027","url":null,"abstract":"<p><strong>Introduction: </strong>The drug \"alkaloids of Sophora flavescens\" (ASF) is an extract from the dried root of S. flavescens Ai. It has various pharmacological effects including anti-arrhythmia, anti-inflammatory, anti-allergic, anti-hepatitis, and antimicrobial. As there are many alkaloids with similar structure and properties, the constituent complexity brings a huge challenge in the quality control of ASF.</p><p><strong>Objective: </strong>To develop new tandem high-performance liquid chromatography (HPLC) fingerprinting methods for the quality control of alkaloids of S. flavescens.</p><p><strong>Methods: </strong>ASF samples were tested based on hydrophilic chromatography and ion suppression chromatographic separation mechanisms, separately. Then, the fingerprints of the two separation mechanisms were established and processed by the computer-aided tandem signal method and tandem data method, respectively. The linear quantitative profiling method was used for both qualitative and quantitative evaluation.</p><p><strong>Results: </strong>The results of the tandem signal method and the tandem data method were accurate and consistent. The tandem signal fingerprint can reveal the inherent characteristics of traditional medicinal materials, and it is better than single fingerprints in terms of signal intensity, separation degree, homogeneity, and information abundance. The tandem data method can rapidly realize the comprehensive quality evaluation of the HPLC fingerprints under different separation mechanisms.</p><p><strong>Conclusion: </strong>These tandem methods overcome experimental compatibility problems and the limitation of the low information content of the single separation mechanism fingerprints and can reveal the distribution and characteristics of the complex chemical components of traditional medicinal materials in a novel, realistic, and holistic way.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"72-84"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}