Phytochemical Analysis最新文献

{"title":"Rapid Identification of Gentiana rigescens Franch. Using Proofman Probe Ladder-Shape Melting Temperature Isothermal Amplification (Proofman-LMTIA) Technology.","authors":"Xiaodong Zhang, Zongding Li, Gaofeng Liu, Xinhui Liu, Chunmei Song, Fugang Xiao, Deguo Wang","doi":"10.1002/pca.3542","DOIUrl":"https://doi.org/10.1002/pca.3542","url":null,"abstract":"<p><strong>Introduction: </strong>Gentiana rigescens Franch. (Jianlongdan) and other Gentiana species (Longdan), including G. manshurica, G. scabra, and G. triflora, are valuable medicinal plants used in traditional Chinese medicine. However, their similar appearances and potential for market misidentification necessitate a rapid and accurate identification method.</p><p><strong>Objective: </strong>This study aimed to develop a rapid identification method using Proofman-LMTIA technology to accurately identify Jianlongdan and prevent misidentification in the market.</p><p><strong>Methods: </strong>Specific primers and Proofman probes were designed based on the differential region of Internal Transcribed Spacer 2 (ITS2) sequence between Jianlongdan and Longdan. The method's specificity, sensitivity, detection limit, and stability were evaluated, and its applicability to commercial gentian products was tested.</p><p><strong>Results: </strong>The developed Proofman-LMTIA method demonstrated high specificity and sensitivity for Jianlongdan at an optimal reaction temperature of 56°C, with a detection limit of 10 pg/μL and the ability to identify 0.1% Jianlongdan in mixtures. The method showed good stability and was successfully applied to market samples.</p><p><strong>Conclusion: </strong>The Proofman ladder-shape melting temperature isothermal amplification (Proofman-LMTIA) method offers a rapid, specific, and cost-effective tool for the authentication of Jianlongdan, crucial for ensuring the quality of herbal medicine and protecting consumers from misidentification and potential adulteration.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Integrated Strategy for Comprehensive Characterization of Traditional Chinese Medicine (TCM) Formulas: A Case Study of Gegen-Qinlian Decoction.","authors":"Zhitian Peng, Chongsheng Peng, Ying Peng, Xiaobo Li","doi":"10.1002/pca.3547","DOIUrl":"https://doi.org/10.1002/pca.3547","url":null,"abstract":"<p><strong>Objective: </strong>An integrated strategy was utilized in this study to comprehensively characterize the chemical composition of Gegen-Qinlian Decoction (GQD).</p><p><strong>Methods: </strong>The major components of GQD (total flavonoids, total saponins, total alkaloids, carbohydrates, and proteins) were determined firstly using colorimetric methods, while free amino acids and trace elements were analyzed by automatic amino acid analyzer and inductively coupled plasma-mass spectrometry (ICP-MS), respectively. After that, the small-molecule components of GQD were characterized using ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS/MS). Furthermore, a quantitative method was established for 26 representative compounds determination simultaneously using ultra-high performance liquid chromatography coupled with triple-quadrupole linear ion-trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) in multiple reaction monitoring (MRM) mode.</p><p><strong>Results: </strong>The accurate content and proportion of the major constituents in GQD were determined for the first time. A total of 230 compounds were characterized from GQD, of which four undescribed alkaloids were identified initially by their tandem mass data analyses based on mass spectral fragmentation pathways of isoquinoline alkaloids. A quantitative method was established to determine 26 representative compounds, and validated through linearity, precision, repeatability, stability, and recovery. The total content of the 26 compounds accounted for 27.81%, of which baicalin being the most abundant was up to 107.26 ± 1 mg/g.</p><p><strong>Conclusions: </strong>The present study achieved a comprehensive characterization of constituents in GQD, laying the foundation for elucidating the active components, mechanism, and the quality control of GQD, offering a valuable analytical framework for research on other traditional Chinese medicine (TCM) formulas.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144209156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the Impact of Extraction Processes on the Quality of Traditional Chinese Medicine Granules.","authors":"Cong Zhang, Chungang Zhang, Meng Liu, Ti Wang","doi":"10.1002/pca.3484","DOIUrl":"10.1002/pca.3484","url":null,"abstract":"<p><strong>Objective: </strong>This review aims to summarize and elucidate the extraction processes of traditional Chinese medicine granules, examining their connection to quality control, with the aim of establishing a foundation for superior extraction methodologies.</p><p><strong>Methodology: </strong>This article systematically collects and analyzes relevant literature from the past two decades through databases such as Science Direct, PubMed, Web of Science, Google Scholar, and CNKI, focusing on the extraction processes of traditional Chinese medicine granules and the factors affecting extraction efficiency.</p><p><strong>Results: </strong>This article provides a comprehensive account of the extraction and granulation processes of traditional Chinese medicine granules. Beginning with the physicochemical properties and chemical components of herbal slices, it explores ways to improve extraction efficiency while also examining the relationship between component structure and biological activity. Rooted in the fundamental theories of traditional Chinese medicine and harmonized with contemporary extraction technologies, it follows the principle that \"quality originates from design,\" ensuring stringent control over the extraction process.</p><p><strong>Conclusion: </strong>This article analyzes extraction technologies, factors influencing extraction efficiency, and quality control measures, establishing a theoretical basis for achieving high-quality and high-yield extraction processes.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"903-911"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142771305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coumarins in the Flavedo of Citrus limon Varieties-Ethanol and Natural Deep Eutectic Solvent Extractions With HPLC-DAD Quantification.","authors":"Fabian Alperth, Bettina Pogrilz, Astrid Schrammel, Franz Bucar","doi":"10.1002/pca.3499","DOIUrl":"10.1002/pca.3499","url":null,"abstract":"<p><strong>Introduction: </strong>Peels are an abundant but still underutilized waste product in the Citrus fruit industry. They contain coumarins with antiadipogenic potential that could be promising targets in new valorization strategies for Citrus peels.</p><p><strong>Objectives: </strong>In this study, these coumarins, that is, citropten, bergamottin, and 5-geranyloxy-7-methoxycoumarin (5G7MC), were investigated in Citrus limon peels of different commercial varieties by HPLC-DAD after extraction with ethanol and choline chloride-based natural deep eutectic solvents (NADES) as alternative extraction agents in green natural product extraction.</p><p><strong>Results: </strong>Target coumarins were almost exclusively found in the flavedo (exocarp) peel layer and in whole peel samples, but not in the albedo (mesocarp) layer. Flavedo samples from varieties \"Verdelli\" (ES) and \"Lisbon\" (ZA) showed particularly high concentrations in ethanolic extracts. When applying NADES extraction, best results for citropten were achieved with malonic acid, 1,2-propanediol, and citric acid as hydrogen bond donors (HBDs), with higher yields than the reference solvent ethanol. For bergamottin and 5G7MC, promising results were obtained for 1,2-propanediol as HBD, though lower yields than with ethanol. The possible chlorination of coumarin epoxides byakangelicol and oxypeucedanin was recognized for acidic NADES in LC-MS analysis.</p><p><strong>Conclusion: </strong>Overall, C. limon peels proved to be a relevant starting material for valorization through coumarin extraction with ethanol and NADES.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"1141-1152"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12129719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of the Major Bioactive Component of Silybum marianum in Nutricosmetics by a HPLC Method With Amperometric Detection and UAE Pretreatment.","authors":"Lucía Abad-Gil, M Jesús Gismera, M Teresa Sevilla, Jesús R Procopio","doi":"10.1002/pca.3478","DOIUrl":"10.1002/pca.3478","url":null,"abstract":"<p><strong>Introduction: </strong>Nutricosmetics derived from Silybum marianum, known for their anti-inflammatory and hepatoprotective properties, necessitate accurate quantification of silybin, a key bioactive component.</p><p><strong>Objectives: </strong>This study aims to develop a novel high-performance liquid chromatography (HPLC) method with amperometric detection (HPLC-ECD) for the precise determination of silybin. An ultrasound-assisted extraction (UAE) procedure is also established for solid sample preparation prior to chromatographic analysis.</p><p><strong>Materials and methods: </strong>Chromatographic separation of silybin was performed on a C18 column and using methanol-0.035 M potassium phosphate (pH 4.0) at 1.0 mL min^-1 flow rate as mobile phase in gradient mode. The electrochemical detection (ECD) of silybin was carried out on a glassy carbon electrode (GCE) at +1.10 V versus Ag/AgCl. The UAE procedure for silybin extraction from solid samples was performed by 15 min sonication in an ultrasonic bath (80 kHz and 100% power) at room temperature.</p><p><strong>Results: </strong>Under the optimal chromatographic conditions, silybin diastereoisomers (silybin A and silybin B) can be separated from other S. marianum flavonolignans in less than 20 min, with a detection limit (LOD) of 0.060 mg L^-1 and a reproducibility (RSD) of 5%. This method was successfully applied to analyze silymarin-containing products with recoveries close to 100%.</p><p><strong>Conclusions: </strong>This work presents the first HPLC method for silybin determination using an amperometric detector with a GCE. The LOD is competitive in comparison with previously published HPLC-DAD methods. This HPLC-ECD method allows silybin diastereoisomers identification without interferences of other flavonolignans present in silymarin extracts.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"934-942"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12129713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HPLC Fingerprint Combined With Chemometrics and Multicomponent Content Determination for Quality Evaluation and Control of Huangma Tincture.","authors":"Jing Qiu, Jie Li, Si-Yang Shang, Peng Zhou, Jing Leng","doi":"10.1002/pca.3487","DOIUrl":"10.1002/pca.3487","url":null,"abstract":"<p><strong>Introduction: </strong>Huangma Tincture (HMT) is a Chinese patent medicine with a history of clinical use for more than 60 years, widely used for treating dermal chronic ulcer such as diabetic foot ulcer. However, the overall quality evaluation and control method of HMT has not yet been researched.</p><p><strong>Objective: </strong>The aim of this study is to establish a comprehensive quality evaluation and control method for HMT based on high-performance liquid chromatography (HPLC) fingerprint, chemometrics, and multicomponent content determination.</p><p><strong>Methods: </strong>Establishing chemical fingerprint of HMT and carrying out similarity analysis comprehensively reflect the consistency of the formulation in terms of chemical composition. Chemometrics analyses, including hierarchical cluster analysis (HCA), principal component analysis (PCA), and orthogonal partial least squares-discriminant analysis (OPLS-DA), were performed to identify components crucial to quality differences. The content of five potential bioactive ingredients (brucine, strychnine, jatrorrhizine, coptisine, and berberine hydrochloride) was determined as a supplementary quality control measure.</p><p><strong>Results: </strong>HPLC fingerprint of HMT with similarity index > 0.990 was established, in which five common compounds (brucine, strychnine, jatrorrhizine, coptisine, and berberine hydrochloride) were identified. HCA, PCA, and OPLS-DA results, validated through 200 permutation tests, were basically consistent. The contents of brucine, strychnine, jatrorrhizine, coptisine, and berberine hydrochloride in 20 batches of HMT samples were 0.2088-0.5556, 0.2599-0.9868, 0.1358-0.2092, 0.2634-0.6843, and 1.8301-2.7826 mg/mL, respectively.</p><p><strong>Conclusion: </strong>HPLC fingerprint combined with chemometrics and multicomponent content determination considering both effect and toxicity provides a robust method for the comprehensive quality evaluation and control of HMT.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"1002-1016"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Vegetable Waste Byproducts of Selected Cultivars of Foeniculum vulgare Mill. by an Integrated LC-(HR)MS and ¹H-NMR-Based Metabolomics Approach.","authors":"Maria Assunta Crescenzi, Antonietta Cerulli, Milena Masullo, Paola Montoro, Sonia Piacente","doi":"10.1002/pca.3488","DOIUrl":"10.1002/pca.3488","url":null,"abstract":"<p><strong>Introduction: </strong>The metabolome of plants is influenced by various factors, especially environmental, as the season in which they are grown. So, distinct varieties of the identical plant might show an increase or decrease in metabolites. The diversity of content of primary and secondary metabolites can also determine the variation in their biological properties. Due to the current occurrence of various fennel varieties, the crop can now be grown for the entire year.</p><p><strong>Objective: </strong>This work used an integrated approach of LC/MS and NMR analysis to characterize the metabolome of fennel waste of different varieties by multivariate statistical analysis.</p><p><strong>Methods: </strong>The extracts were investigated by NMR and LC/MS analysis to focus attention on the primary and secondary metabolites. Both LC-HRMS and NMR data were analyzed by principal component analysis (PCA).</p><p><strong>Results: </strong>The ¹H-NMR analysis led to the identification of 15 primary metabolites, such as amino acids, carbohydrates, and organic acid derivatives. The secondary metabolites identified by LC/MS analysis mainly belong to the phenolic, lipid, and fatty acid compounds classes.</p><p><strong>Conclusion: </strong>This integrated approach guarantees a precise and complete overview of the variations in the metabolic expression of the fennel varieties grown in different seasons.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"1017-1030"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12129718/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality Markers of Fufang Shenqi Oral Liquid Based on Integrated Fingerprint Analysis, Chemical Pattern Recognition, and Quantification.","authors":"Yingqi Zhang, Yangling Li, Yanwei Cheng, Huiling Nan, Yuqiang Wu, Hongtao Chen, Xuejian Li, Yudong Luo, Anqiang Tan, Qing Chen","doi":"10.1002/pca.3489","DOIUrl":"10.1002/pca.3489","url":null,"abstract":"<p><strong>Introduction: </strong>Fufang Shenqi Oral Liquid (FFSQOL) is an important Chinese medicine compound preparation with a wide range of clinical applications, which is mainly used to regulate immune function, improve cardiovascular function, and have anti-inflammatory and antibacterial effects. At present, it is of great importance to establish the quality evaluation method of FFSQOL and to investigate its quality markers (Q-markers).</p><p><strong>Objectives: </strong>The aim of this study is to establish a quality evaluation method for FFSQOL and screen its Q-markers to provide a scientific basis for its quality control.</p><p><strong>Methods: </strong>Fourteen batches of FFSQOL were subjected to high-performance liquid chromatography (HPLC) fingerprint and similarity analysis. The components of FFSQOL were identified, and their content was determined. This was combined with cluster analysis (CA) and principal component analysis (PCA) to determine the Q-markers of FFSQOL.</p><p><strong>Results: </strong>In this study, an HPLC fingerprint was established for 14 batches of FFSQOL, identifying 12 common peaks and six major components. Four components were identified as stable and reproducible: gallic acid (504.94 ~ 1219.04 μg/mL), caffeic acid (452.15 ~ 783.01 μg/mL), 7-O-glucoside (1097.72 ~ 2440.41 μg/mL), and formononetin (176.2 ~ 177.51 μg/mL). Quality evaluation of the 14 batches was conducted using chemical pattern recognition analysis. CA results indicated two distinct groups, and PCA revealed that principal components 1 and 2 were the main factors influencing batch differences. A combination of HPLC fingerprint, content determination results, and chemical pattern recognition analysis was employed to identify Q-markers for FFSQOL. The markers identified were gallic acid, caffeic acid, calycosin 7-O-glucoside, and formononetin.</p><p><strong>Conclusion: </strong>In this study, a quality evaluation method for FFSQOL was established through the implementation of fingerprint, content determination, and chemical pattern recognition analysis, resulting in the identification of four Q-Markers of FFSQOL, which laid the foundation for the formulation of FFSQOL quality standards.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"1031-1040"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating UPLC-MS/MS Bioinformatics and In Vivo Experiments Validation to Elucidate the Mechanism of Wenzi Jiedu Decoction in Suppressing Colorectal Cancer.","authors":"Tianqing Sang, Aoshengxiong Liang, Hongli Zhou, Wenli Qiu, Lijing Shi, Hongguang Zhou","doi":"10.1002/pca.3495","DOIUrl":"10.1002/pca.3495","url":null,"abstract":"<p><strong>Objectives: </strong>We used ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS), bioinformatics, and in vivo experiments to study the anti-colorectal cancer (CRC) effects of Wenzi Jiedu Decoction (WJD).</p><p><strong>Methods: </strong>Detected the main components of WJD by UPLC-MS/MS. Obtained WJD targets and CRC targets through the open source database. Analyzed the WJD-CRC targets from a macro perspective by PPI, GO, and KEGG analyses. Validated bioinformatics findings by molecular docking and animal experiments.</p><p><strong>Results: </strong>This study obtained 91 active compounds and 240 targets of WJD. Intersection with CRC genes (GSE32323 388 DEGs, GSE 215510 1253 DEGs), 36 WJD-CRC common targets were obtained. PPI and enrichment analyses indicated WJD exerted anti-CRC effects mainly through the chemokine signaling pathway and apoptosis. Quercetin, Luteolin, Kaempferol, Formononetin, Stigmasterol, and Hederagenin were the main compounds of WJD. CXCL8, BCL-2, BAX, BCL2L1, CASP3, AKT1, and TP53 were the core targets of WJD-CRC. Bulk molecular docking showed that core WJD compounds had good docking activity with WJD-CRC targets. Animal experiments had shown the tumor inhibition rate of the WJD group was 36.53%. WJD could regulate the ratio of CD4+, CD8+, and CD4+/CD8+, reduce the expression of CXCL8, and BCL-2, and increase the expression of BAX.</p><p><strong>Conclusions: </strong>This study indicated that the potential mechanism of WJD in the prevention and treatment of CRC had the characteristics of the multi-target, multi-path, and multi-system mechanisms, which were mainly related to the regulation of chemokines and the promotion of apoptosis.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"1105-1117"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality Evaluation of Wenyujin Rhizoma Concisum From Different Districts in China Based on HPLC, Heracles NEO Ultrafast Gas-Phase Electronic Nose, and FT-NIR.","authors":"Sichen Wang, Kewei Zhang, Xiuqi Gan, Tianfei Ma, Tulin Lu, Chunqin Mao","doi":"10.1002/pca.3506","DOIUrl":"10.1002/pca.3506","url":null,"abstract":"<p><strong>Introduction: </strong>Wenyujin Rhizoma Concisum, named as Pian Jianghuang (PJH) in China, is the decoction piece from the dried rhizome of Curcuma wenyujin Y. H. Chenet C. Ling, has been used to relieve pain for many years in China. However, the qualities of PJH in different districts differ greatly due to their growing environments, which would affect their clinical applications.</p><p><strong>Objective: </strong>To evaluate the qualities of PJH from different districts in China.</p><p><strong>Methods: </strong>HPLC, Heracles NEO ultrafast gas-phase electronic nose, and Fourier transform near-infrared (FT-NIR) spectroscopy were applied to estimate the qualities of PJH from different districts in China.</p><p><strong>Results: </strong>By HPLC, the average contents of neocurdione, curdione, germacrone, and furanodiene were 0.203%, 0.151%, 0.022%, and 0.022% in Fujian (FJ); 0.447%, 0.786%, 0.298%, and 0.276% in Zhejiang (ZJ); 0.082%, 0.259%, 0.038%, and 0.019% in Yunnan (YN); 0.041%, 0.022%, 0.260%, and 0.024% in Guangxi (GX); and 0.026%, 0.091%, 0.260%, and 0.016% in Anhui (AH), respectively. By Heracles NEO ultrafast gas-phase electronic nose, the unique odor components in PJH from FJ, YN, and GX were 1,2,4-thiadiazole,5-ethoxy-3-(trichloromethyl), 5,6,7,8-tetrahydroquinoxaline, and 1,3,5-trimethylbenzene, respectively, while ZJ and AH both contained two unique odor components, respectively: pentyl pentanoate and dill ether (ZJ), myrcene, and 2-pentadecanone (AH). Moreover, PJH from above five districts could be discerned quickly by FT-NIR.</p><p><strong>Conclusion: </strong>The application of multidimensional analytical techniques in quality assessment of PJH from different districts in China would provide a new idea for quality control and geographical origin traceability of traditional Chinese materia medica.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"1231-1244"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}