Phytochemical Analysis最新文献

{"title":"Aloe vera Enhanced Hydroxycamptothecin-Mediated Antitumor Activity in Colorectal Carcinoma Based on the Targeted Regulation of Nucleotide Metabolism.","authors":"Cai-Yun Wang, Yan Li, Wei Zhang, Zhi-Hong Jiang","doi":"10.1002/pca.70096","DOIUrl":"https://doi.org/10.1002/pca.70096","url":null,"abstract":"<p><strong>Introduction: </strong>Colorectal cancer (CRC) remains one of the leading causes of cancer-related mortality worldwide. Combination therapies, which integrate traditional Chinese medicine (TCM) with conventional chemotherapeutic agents, offer a promising new strategy for the management of CRC.</p><p><strong>Objective: </strong>This study aimed to investigate the potential of Aloe vera (L.) Burm.f. (A. vera) as an adjuvant to hydroxycamptothecin (HCPT) for CRC treatment and to explore the underlying mechanisms, specifically focusing on nucleotide metabolism, as perturbations in intracellular nucleotide pools are closely linked to various human pathologies.</p><p><strong>Methods: </strong>The aqueous extract of A. vera adjuvantly ameliorated the progression of colon carcinogenesis was investigated in Apc^Min/+ mouse model. For the underlying mechanism studies, cytotoxicity assessment, DNA damage, cell cycle, RNA and DNA synthesis analysis, endogenous nucleotides determination, and Western blot were performed in HCT 116 cells.</p><p><strong>Results: </strong>Major constituents of A. vera, including aloin A, aloin B, aloesin, aloenin, and aloe-emodin, were quantified and found to meet Chinese Pharmacopoeia standards. A. vera synergistically enhanced the anticancer activity of HCPT. This effect was mediated by exacerbated DNA damage, imbalanced purine/pyrimidine metabolism, and inhibition of the Wnt/β-catenin signaling pathway.</p><p><strong>Conclusion: </strong>This study provided preliminary evidence that A. vera could be developed as an adjuvant therapy for CRC, contributing to its further clinical application in this field.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149220591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous Screening and Identification of Potential Bioactive Components Against MASLD in Guizhi Fuling Decoction by Integrating a Multitarget Affinity Ultrafiltration Strategy With UPLC-Q-TOF-MS/MS.","authors":"Zhou Fang, Renai Fu, Meng Zhang, Yingqiu Ma, Liping Wu, Yongbing Xu, Shibo Li, Mingquan Guo","doi":"10.1002/pca.70094","DOIUrl":"https://doi.org/10.1002/pca.70094","url":null,"abstract":"<p><strong>Introduction: </strong>As one of the most common chronic liver diseases worldwide, metabolic dysfunction-associated steatotic liver disease (MASLD) imposes an escalating burden on global health systems. However, the efficacy of Guizhi Fuling decoction (GZFLD) against MASLD and its potential pharmacodynamic material basis remain elusive.</p><p><strong>Objectives: </strong>This work aims to explore the potential bioactive constituents of GZFLD against MASLD by integrating a multitarget affinity ultrafiltration strategy with UPLC-Q-TOF-MS/MS.</p><p><strong>Methods: </strong>The efficacy of GZFLD against MASLD was initially evaluated using an in vitro HepG2 model of MASLD. Subsequently, potential active ingredients of GZFLD against MASLD were screened out via affinity ultrafiltration coupled with liquid chromatography-tandem mass spectrometry (AUF-LC-MS/MS) targeting multiple enzymes, including pancreatic lipase (PL), cyclooxygenase-2 (COX-2), proprotein convertase subtilisin/kexin type 9 (PCSK9), and interleukin-6 (IL-6). Finally, molecular docking simulations, enzyme inhibition assays, and cell experiments were performed to validate the activities of the candidate components.</p><p><strong>Results: </strong>The results demonstrated that GZFLD significantly alleviated lipid accumulation and oxidative stress in the HepG2 model of MASLD, and a total of 18 potential bioactive ingredients were identified in GZFLD. Among them, benzoylpaeoniflorin, galloylpaeoniflorin, and ellagic acid are the main active compounds of GZFLD against MASLD. Furthermore, the results of molecular docking revealed that these components could strongly bind their corresponding target by various forces. The result of COX-2 inhibition assay indicated that ellagic acid exhibited a strong COX-2 inhibitory activity. Moreover, the results of cell verifications implied that ellagic acid and benzoylpaeoniflorin exhibited significant therapeutic potential on MASLD by modulating lipid metabolism and inflammatory responses.</p><p><strong>Conclusion: </strong>GZFLD demonstrated noteworthy effects against MASLD by alleviating lipid accumulation and oxidative stress, and the potential bioactive components of GZFLD and their interaction network against MASLD were first explored and constructed by combining AUF-LC-MS/MS with multiple targets, which showcased a powerful new integrating strategy for the simultaneous screening and identification of bioactive compounds in complex Chinese medicines.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149220594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics: A Unique Approach for Chemical Profiling of Plants-Derived Secondary Metabolites and Its Application in Plant Sciences.","authors":"Chandra Prakash, Pebi Rime, Ashish Bahuguna, Shivani Uniyal, Amit Anthwal, Rohit Mahar","doi":"10.1002/pca.70093","DOIUrl":"https://doi.org/10.1002/pca.70093","url":null,"abstract":"<p><strong>Introduction: </strong>In-depth profiling of plant-derived metabolites is essential for unraveling chemically diverse group of bioactive compounds that play essential roles in plant defense, adaptation, ecological interactions, and human health. Comprehensive profiling of these metabolites is important for understanding their biochemical diversity and exploring their pharmaceutical, agricultural, and industrial potentials. Metabolomics is an emerging platform for qualitative and quantitative assessment of metabolites, employing GC-MS, LC-MS, and NMR.</p><p><strong>Objective: </strong>To compare and critically assess the extraction, data acquisition, and data-processing protocols including chemometric analysis and applications of metabolomics.</p><p><strong>Methodology: </strong>Metabolomics studies largely depend on optimized sample collection, extraction procedures, analytical platforms, and data-processing workflows for the chemical profiling of plant-derived metabolites. Following metabolite extraction from different plant parts, analytical data generated by GC-MS, LC-MS, and NMR are further analyzed, employing chemometric statistical approaches for identification of metabolic signatures, classification of plant samples, and to reveal biochemical variations associated with genetic and environmental factors.</p><p><strong>Results: </strong>This review focuses on several applications of metabolomics in plant sciences, including elucidating plant responses to abiotic and biotic stresses, evaluating the effects of nutrient availability on metabolite biosynthesis, investigating genetic and phenotypic diversity among wild-type, cultivated, and transgenic plants, supporting crop improvement and metabolic engineering, authenticating medicinal plants, ensuring quality control, and accelerating the discovery of bioactive natural products.</p><p><strong>Conclusion: </strong>This review provides comprehensive comparison of various extraction and characterization techniques of secondary metabolites, which will provide valuable insights as well as further directions to researchers working in allied field.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149183578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Stability and Pharmacokinetic Evaluation of 12 Compounds in Qingzhuan Dark Tea in Rat Models.","authors":"Xiaolei Miao, Haoyang Quan, Dan Ye, Min Zheng, Yiye Zhao","doi":"10.1002/pca.70092","DOIUrl":"https://doi.org/10.1002/pca.70092","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to systematically evaluate the in vitro stability and pharmacokinetic behavior of representative polyphenols in Qingzhuan dark tea (QDT) and to explore the relationship between physicochemical stability and systemic exposure.</p><p><strong>Methods: </strong>Twelve representative polyphenols, including eight catechins and four theaflavins, were evaluated using a three-factor, three-level L₉ orthogonal design to investigate the effects of pH, temperature, and time on stability. Based on stability and plasma detectability, seven catechins were further investigated in male Sprague-Dawley rats following oral administration of QDT extract at doses of 1, 2, and 3 g/kg. A validated liquid chromatography-mass spectrometry (LC-MS) method was used for quantitative analysis and pharmacokinetic evaluation.</p><p><strong>Results: </strong>The optimal preservation condition for the QDT extract and mixed standards was pH 5, 4°C, and 30 min. The analytical method showed satisfactory linearity (r > 0.9908), precision (< 9.82%), accuracy (90.2%-109.8%), recovery (63.9%-90.8%), and matrix effects (87.64%-108.76%). Seven catechins were detected in rat plasma, whereas theaflavins were not detected under the present experimental conditions. C_max and AUC generally increased with increasing dose, indicating dose-dependent systemic exposure.</p><p><strong>Conclusion: </strong>The integrated stability and pharmacokinetic evaluation establishes a link between physicochemical stability and biological disposition of QDT polyphenols, providing a scientific basis for QDT storage optimization, quality control, formulation development, and functional application.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149113559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction and Application of an Integrated \"Chemical-Biological\" Dual-Core Quality Evaluation System for Salvia miltiorrhiza.","authors":"Xiaoyue Min, Zijia Zhang, Qianyue Liang, Yang Zhou, Huali Long, Jinjun Hou, De-An Guo, Wanying Wu","doi":"10.1002/pca.70091","DOIUrl":"https://doi.org/10.1002/pca.70091","url":null,"abstract":"<p><strong>Background and aim: </strong>Practical implementation of integrated chemical-biological quality control in Traditional Chinese Medicine (TCM) remains challenging. This study used Salvia miltiorrhiza as a model to develop an exploratory integrated quality-evaluation strategy that combines chemical profiling with a standardized in vitro relative-potency assay.</p><p><strong>Methods: </strong>Chemical characterization of 58 batches included UPLC fingerprinting and quantification of 10 constituents. Relative biological potency was evaluated using a parallel-line assay in an H9c2 cardiomyoblast oxygen-glucose deprivation/reoxygenation (OGD/R) model.</p><p><strong>Results: </strong>Across the 58 commercial batches, fingerprint similarity ranged from 0.469 to 0.997 and the total content of the 10 quantified compounds ranged from 1.36% to 8.27%. Total tanshinones showed greater interbatch variation (CV = 55.2%) than total phenolic acids (CV = 46.2%). Four batches did not satisfy the statistical validity criteria of the parallel-line model and were therefore nonreportable; two of these batches showed no conspicuous abnormality in the routine quantitative indicators. Among the 54 reportable batches, relative potency ranged from 57.6 to 276.5 U/mg and correlated moderately with total quantified content (r = 0.719, p < 0.001). Partial least squares regression identified salvianolic acid B, rosmarinic acid, cryptotanshinone, and tanshinone IIA as chemical variables statistically associated with the in vitro response. The median-based four-quadrant analysis flagged chemical-biological discordance in 10 of 54 batches (18.5%).</p><p><strong>Conclusion: </strong>The integration of chemical profiling with a standardized cell-based relative potency assay enabled multidimensional assessment of S. miltiorrhiza quality by simultaneously characterizing batch composition, biological response, and their concordance. This approach provides a practical basis for identifying quality variations that may not be captured by chemical analysis alone and supports the development of more comprehensive quality-evaluation strategies for botanical drugs.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149113609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperspectral Camera Imaging Tandem With HPLC-UV-ELSD Determination and Cell Bioassay for Geographical Discrimination and Quality Consistency Evaluation of Anoectochilus roxburghii.","authors":"Haixia Xu, Qiuya Zhou, Qiluo Ni, Weiyue Hu, Xiangwei Xu, Yi Tao","doi":"10.1002/pca.70086","DOIUrl":"10.1002/pca.70086","url":null,"abstract":"<p><strong>Introduction: </strong>Anoectochilus roxburghii (AR) is a prized medicinal herb valued for its hepatoprotective effects. Its quality varies depending on geographical origin. The primary bioactive constituents include rutin, quercetin-7-O-glucoside, kaempferol-3-O-rutinoside, narcissin, quercetin, and kinsenoside. A method that enables simultaneous determination of both the content and bioactivity of the herb is therefore essential for effective quality control.</p><p><strong>Objective: </strong>To develop a rapid, nondestructive approach for simultaneously predicting the contents of primary bioactive constituents and hepatoprotective effects of AR using hyperspectral camera imaging (HCI) combined with deep learning models.</p><p><strong>Method: </strong>Hyperspectral images of 100 AR batches were acquired using a portable Vis-NIR HCI system (389.81-1048.18 nm). The contents of six active compounds were quantified via HPLC-UV and HPLC-ELSD, while hepatoprotective activity was evaluated using an APAP-induced L02 cell injury model. Quantitative calibration models were constructed using partial least squares regression (PLSR) and the following three deep learning architectures: liquid neural network (LNN), Mamba state space model, and graph convolutional network (GCN). Their predictive performances were systematically compared. Shewhart control charts were employed to visualize batch-to-batch quality variation.</p><p><strong>Result: </strong>The Mamba model demonstrated superior predictive performance across all seven quality attributes, achieving the highest coefficients of determination (R_p ² up to 0.9972) and the lowest prediction errors. It significantly outperformed PLSR, LNN, and GCN models. Furthermore, the integration of Shewhart charts enabled effective visualization of quality consistency across batches.</p><p><strong>Conclusion: </strong>This study presents a novel HCI-Mamba framework designed for the rapid, nondestructive, and multicomponent quality assessment of AR. The proposed strategy offers a high-throughput solution for AR quality control while providing a methodological framework applicable to other complex herbal medicines.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"969-979"},"PeriodicalIF":2.9,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148759977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Binding Ability of Aloe-Emodin to Free l-Cysteine on Hepatic Protein-Induced Hepatocyte Toxicity.","authors":"Lingwen Dong, Yanan Liu, Lingge Shi, Boyan Liu, Shiqi Qiu, Xinhang Zhang, Wuxiyar Otkur, Guoxiang Sun, Xu Wang","doi":"10.1002/pca.70083","DOIUrl":"10.1002/pca.70083","url":null,"abstract":"<p><strong>Introduction: </strong>Aloe-emodin (AE), as an anthraquinone active component in traditional Chinese medicine, exhibits multiple pharmacological activities, yet its mechanism of hepatotoxicity remains unclear. The purpose of this study was to systematically investigate the molecular mechanism of AE-induced hepatotoxicity, with a specific focus on hepatic protein adduction.</p><p><strong>Materials and methods: </strong>AE was incubated with murine hepatic protein solution, followed by enzymatic digestion and liquid chromatography-tandem mass spectrometry analysis to detect l-cysteine covalent adducts. For in vivo study, mice were administered AE, and the formation of hepatic protein adduction was monitored over time and across different doses. Additionally, buthionine sulfoximine was used as a pretreatment to assess the role of glutathione in the adduction process. Cell experiments were also performed to evaluate the relationship between protein adduct formation and cytotoxicity.</p><p><strong>Results: </strong>AE-cysteine covalent adducts A1 and A2 were identified both in vitro and in livers of AE-treated mice. In vivo study confirmed dose-dependent formation of AE-cysteine adducts (A1/A2) in mouse liver, peaking at 30 min. Depleting glutathione elevated A1 and A2 levels by 40.43% and 27.93% in vivo, indicating the protective role of glutathione through competitively conjugating with AE, thereby reducing its covalent modification of hepatic proteins. Cytotoxicity of AE is positively correlated with its concentration, which reveals the critical role of adduct formation in initiating dosage-dependent cytotoxicity.</p><p><strong>Conclusion: </strong>This study demonstrates that hepatotoxicity of AE is directly associated with the formation of covalent adducts targeting l-cysteine residues, providing a critical theoretical foundation for safety evaluation and clinical application of AE-containing herbs.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"957-968"},"PeriodicalIF":2.9,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148437600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Exosome-Based Therapy for Cardiovascular Disease: Traditional Chinese Medicine Offering New Avenues for Exosome Functionalization.","authors":"Yi Chen, Xiaoyu Sun, Yingbo Ouyang, Rong Miao, Atinga Vallada, Rui Liu, Jingwen Guo, Chuwen Wang, Shiqi Zhang, Lin Li, Zhe Gao, Qilong Wang, Han Zhang, Ling Leng","doi":"10.1002/pca.70085","DOIUrl":"10.1002/pca.70085","url":null,"abstract":"<p><strong>Background: </strong>Cardiovascular diseases (CVDs) remain a leading cause of global mortality and impose a substantial health and economic burden worldwide. Exosomes, as promising endogenous nanocarriers, have emerged as a powerful tool for the prevention and treatment of CVDs. In particular, advanced functionalization strategies have largely enhanced exosomal therapeutic efficacy in vivo. Notably, Traditional Chinese Medicine (TCM) and its bioactive components exert profound regulatory effects on exosomes.</p><p><strong>Methods: </strong>In this review, we systematically summarize exosome-based therapeutic strategies for CVDs, along with state-of-art functionalization approaches to optimize exosomal cargo loading and targeted delivery. We further provide a comprehensive overview of TCM-mediated exosomal regulation.</p><p><strong>Results: </strong>We found that TCM and TCM-derived chemicals can optimize exosomal cargo loading, especially the loading of microRNAs (miRNAs) and bioactive chemicals. More importantly, TCM and chemicals can promote exosomal secretion, which provides new avenues for exosomal-scale production. Besides, there are synergistic effects between exosomes and TCM when co-administered.</p><p><strong>Conclusion: </strong>Collectively, exosome-based systems hold great promise for CVD therapy, and TCM provides novel strategies for exosomal functionalization, which substantially enhances exosomal-mediated therapeutic efficacy for CVDs.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"936-956"},"PeriodicalIF":2.9,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148663273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant-Derived Extracellular Vesicles (PDEVs): A Natural Nanoplatform Reshaping the Therapeutic Potential of Medicinal and Edible Plants.","authors":"Hongzhe Shen, Yanzhi Xiong, Mengjia Xu, Mingquan Guo","doi":"10.1002/pca.70084","DOIUrl":"10.1002/pca.70084","url":null,"abstract":"<p><strong>Background: </strong>Medicinal and edible plant-derived compounds have held an important position in human history due to their therapeutic effects. However, their therapeutic potential is limited by their inherent poor stability, low bioavailability, and insufficient targeting. Plant-derived extracellular vesicles (PDEVs) have recently emerged as a promising natural nanoplatform to address these challenges.</p><p><strong>Objectives: </strong>This review systematically summarizes the latest research on PDEVs from medicinal and edible plants, covering their biogenesis mechanisms, preparation methods, analysis methods, and biological applications. We aim to establish a theoretical foundation for the applied research of PDEVs.</p><p><strong>Methods: </strong>Recent literature in the field of PDEVs was comprehensively reviewed, focusing on preparation strategies, analytical techniques, and therapeutic applications in various disease models.</p><p><strong>Results: </strong>This study systematically reviews the main biogenesis pathways of PDEVs and comparatively analyzes the technical characteristics of different preprocessing, separation, and purification methods. Through a series of complementary analytical techniques, we can perform comprehensive and reliable multidimensional characterization of the physical properties and chemical composition of PDEVs. A large number of preclinical studies have shown that PDEVs possess anti-inflammation, tissue repair, and anticancer effects, realized through the regulation of multiple signaling pathways.</p><p><strong>Conclusion: </strong>PDEVs, as a highly promising natural nanodelivery platform, can effectively address many obstacles faced by traditional plant-derived compounds. Although considerable challenges remain, ongoing research underscores the potential of PDEVs to open up new and transformative therapeutic applications for the development and utilization of medicinal and edible plants.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"910-935"},"PeriodicalIF":2.9,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148606488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Diversity and Evolving Strategies for the Quality Evaluation of Agarwood.","authors":"Yanqiao Xie, Shengqi Huang, Yingmin Zhang, Ruonan Tong, Zhuzhen Han, Wenxiang Fan, Zhengtao Wang, Hechen Wang, Linnan Li, Li Yang","doi":"10.1002/pca.70082","DOIUrl":"10.1002/pca.70082","url":null,"abstract":"<p><strong>Background: </strong>Agarwood, a valuable resin-infused wood derived primarily from Aquilaria species (Thymelaeaceae), is widely used in traditional medicine, incense, perfumery, and healthcare products. Its growing scarcity, along with the demand for robust quality control, has driven extensive research, supported by advances in analytical technologies. Recent efforts have focused on elucidating the scientific basis of its traditional applications and promoting sustainable utilization.</p><p><strong>Objectives: </strong>This review aims to consolidate research progress of agarwood, encompassing updates on agarwood resources, newly identified chemical constituents (2020-2026), emerging analytical strategies, and recent pharmacological findings. It further seeks to outline future research directions to address existing challenges in quality evaluation and mechanistic understanding.</p><p><strong>Methods: </strong>A systematic retrieval of recent literatures pertaining to agarwood was conducted. Through data extraction and integrative analysis, this review synthesizes the latest research progress and identifies prevailing trends in the field.</p><p><strong>Results: </strong>Recent phytochemical studies have identified over 200 new chemical constituents (2020-2026), including structurally diverse dimeric compounds. Integrated analytical strategies combining spectroscopic, chromatographic, and sensor-based techniques have greatly improved the authentication, sensory evaluation, and grading of agarwood. Additionally, a broad spectrum of pharmacological activities attributable to agarwood has been extensively corroborated.</p><p><strong>Conclusion: </strong>This review documents recent advancements concerning the chemical compositions, analytical strategies, and pharmacological activities of agarwood. Challenges remain in mechanisms, structure-activity relationships, and compositional variation, hindering standardization. It will be essential for future work to deepen mechanistic insights and to identify key quality markers, thereby advancing the fields of quality control and translational research for agarwood.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"879-909"},"PeriodicalIF":2.9,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148796924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}