Phytochemical Analysis最新文献

{"title":"Quality Consistency Monitoring of Alkaloids of Sophora flavescens by Tandem High-Performance Liquid Chromatography Fingerprinting and Linear Quantitative Profiling Method.","authors":"Zhifei Hou, Yongzhen Chang, Jing Zhang, Yan Li, Guoxiang Sun","doi":"10.1002/pca.70027","DOIUrl":"https://doi.org/10.1002/pca.70027","url":null,"abstract":"<p><strong>Introduction: </strong>The drug \"alkaloids of Sophora flavescens\" (ASF) is an extract from the dried root of S. flavescens Ai. It has various pharmacological effects including anti-arrhythmia, anti-inflammatory, anti-allergic, anti-hepatitis, and antimicrobial. As there are many alkaloids with similar structure and properties, the constituent complexity brings a huge challenge in the quality control of ASF.</p><p><strong>Objective: </strong>To develop new tandem high-performance liquid chromatography (HPLC) fingerprinting methods for the quality control of alkaloids of S. flavescens.</p><p><strong>Methods: </strong>ASF samples were tested based on hydrophilic chromatography and ion suppression chromatographic separation mechanisms, separately. Then, the fingerprints of the two separation mechanisms were established and processed by the computer-aided tandem signal method and tandem data method, respectively. The linear quantitative profiling method was used for both qualitative and quantitative evaluation.</p><p><strong>Results: </strong>The results of the tandem signal method and the tandem data method were accurate and consistent. The tandem signal fingerprint can reveal the inherent characteristics of traditional medicinal materials, and it is better than single fingerprints in terms of signal intensity, separation degree, homogeneity, and information abundance. The tandem data method can rapidly realize the comprehensive quality evaluation of the HPLC fingerprints under different separation mechanisms.</p><p><strong>Conclusion: </strong>These tandem methods overcome experimental compatibility problems and the limitation of the low information content of the single separation mechanism fingerprints and can reveal the distribution and characteristics of the complex chemical components of traditional medicinal materials in a novel, realistic, and holistic way.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive Spirocyclic C-Arylglycosides From the Fungal-Fungal Co-Culture of Pestalotiopsis sp. and Nigrospora sp.","authors":"Meifang Wu, Yang Jin, Ying Chen, Ziqian Zeng, Wenbin Deng, Xiangdong Su, Yongbo Xue","doi":"10.1002/pca.70024","DOIUrl":"https://doi.org/10.1002/pca.70024","url":null,"abstract":"<p><strong>Introduction: </strong>In this study, two new papulacandin analogs (1-2), together with 12 known compounds (3-14), were identified from the co-culture of two endophytic fungi, Pestalotiopsis sp. J025 and Nigrospora sp. Wn-2-2, which were isolated from Wikstroemia nutans Champ. ex Benth.</p><p><strong>Objectives: </strong>The aim of this study is to investigate the antifungal activity of papulacandins and other secondary metabolites from the co-culture of two endophytic fungi.</p><p><strong>Material and methods: </strong>Their chemical structures, including relative configurations, were elucidated using extensive spectroscopic analyses, including HRESIMS, 1D and 2D NMR, ECD calculations, and quantum mechanical-NMR calculations, as well as comparison with reported analogs.</p><p><strong>Results: </strong>Compound 3 demonstrated promising in vitro antifungal activity against fluconazole-resistant Candida albicans with a minimum inhibitory concentration in 50% isolates (MIC₅₀) of 0.57 ± 0.01 μM.</p><p><strong>Conclusion: </strong>These findings demonstrate that the papulacandin family is a promising resource for the development of antifungal agents against invasive Candida infections.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144964899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted Submetabolome Profiling of Bufadienolides in Venenum Bufonis Using Scheduled Diagnostic Product Ion-Pair Filtering and Substructure Recognition-Based Strategy.","authors":"Xuelin Sun, Wei Ding, Zhanpeng Shang, Jiantao Qiu, Xueying Tan","doi":"10.1002/pca.70025","DOIUrl":"https://doi.org/10.1002/pca.70025","url":null,"abstract":"<p><strong>Introduction: </strong>Venenum bufonis (VB), a traditional animal-derived medicine, exhibits significant antitumor, immunomodulatory, and analgesic activities that are primarily attributed to bufadienolides.</p><p><strong>Objective: </strong>This study presented a novel targeted submetabolomics strategy for comprehensive profiling of bufadienolides in VB.</p><p><strong>Material and methods: </strong>This targeted submetabolome mainly integrated scheduled diagnostic product ion (DPI) pair-based filtering and substructure recognition-based structural assembly based on the LC-MS platform. Key steps included 1) stepwise multiple ion monitoring (MIM) coupled with a dynamic exclusion function to enhance detection of minor peaks; 2) automated annotation of unconjugated bufadienolides via high-resolution extracted ion chromatograms (EICs) and a Microsoft Excel-based platform; 3) establishment of an extended tail database for conjugated bufadienolides (including dicarboxylic acid and dicarboxylic acid-amino acid conjugates) based on structural similarity and derivativity, enabling untargeted screening via validated or scheduled DPI pairs derived from MS/MS fragmentation patterns; and 4) potential conjugated bufadienolides were characterized via a substructure recognition-based strategy.</p><p><strong>Results: </strong>The strategy identified 162 bufadienolides (39 primary, 39 secondary, and 84 tertiary) with a 96% annotation rate (155 characterized). Notably, 81 potential novel compounds were discovered, including 11 previously undescribed structural subclasses.</p><p><strong>Conclusions: </strong>This targeted submetabolome strategy significantly expanded the diversity of bufadienolides in VB and also advanced the application of mass spectrometry in natural product discovery.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144964934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Lavender Essential Oil and Phenolic Yield: Advances in Extraction, Metabolomics, and Functional Benefits.","authors":"Shaza H Aly, Haroon Khan, Mohamed A Farag","doi":"10.1002/pca.70023","DOIUrl":"https://doi.org/10.1002/pca.70023","url":null,"abstract":"<p><strong>Introduction: </strong>Lavender (Lavandula angustifolia) is valued in the food and pharmaceutical industries for its unique aroma, owing to oxygenated monoterpenoids such as linalool, linalyl acetate, lavandulyl acetate, 1,8-cineole, and terpinene-4-ol, which contribute to the quality of lavender essential oil (LEO). It is rich in flavonoids and phenolics like rosmarinic acid. LEO and phenolic yields depend on agronomic attributes, processing techniques, and extraction methods.</p><p><strong>Objectives: </strong>This review aims to provide a comprehensive overview of the newly developed technologies and their optimization parameters to maximize oil yield, aroma quality, and phenolic content.</p><p><strong>Methods: </strong>Related sufficient evidence has been collected from prominent scientific databases, including PubMed, Web of Science, ScienceDirect, and Google Scholar. Data concerning the phytochemistry, biological impacts, agricultural aspects, and processing approaches of Lavender were collected and assessed.</p><p><strong>Results: </strong>The diverse aspects of LEO production, including agricultural factors, climatic conditions, and processing techniques, are reviewed to identify the optimal parameters. Furthermore, technologies including GC/MS, HPLC/MS, and IR highlight the relevance of metabolomics in lavender quality control and analysis. In addition, lavender's anti-inflammatory, antioxidant, and enzyme-inhibiting properties are highlighted.</p><p><strong>Conclusions: </strong>Metabolomic analyses offer comprehensive insights into the biochemical composition of lavender, allowing researchers to identify metabolites linked to favorable agricultural traits, disease resistance, optimized oil extraction, superior quality attributes, and potential nutraceutical value; these data-driven findings guide targeted breeding, cultivation strategies, and product development. Lavender (L. angustifolia) is widely recognized for its aroma and health-promoting compounds, including monoterpenoids and phenolics like rosmarinic acid. Essential oil yield and quality depend on cultivation methods and extraction techniques. This review evaluates recent technological advances to optimize oil production, aroma, and phenolic content, using metabolomics techniques to comprehensively analyze lavender constituents. This comprehensive profiling reveals lavender's defensive mechanisms by linking its constituents to considerable health benefits. The findings help improve lavender quality and use in health and well-being.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144964936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LC-MS-Based Serum Pharmacochemistry Combined With Network Pharmacology and Pharmacodynamics to Evaluate the Role of Macromolecular Components in TCMs' Decoction: A Case Study on Qi-Huo-Yi-Fei Decoction for COPD Therapy.","authors":"Peng Yang, Hu-Dong Lv, Jie Wu, Fang Long, Jing Zhou, Cheng-Ying Wu, Jin-Di Xu, Shan-Shan Zhou, Qing-Ling Xiao, Song-Lin Li, Hong Shen","doi":"10.1002/pca.70021","DOIUrl":"https://doi.org/10.1002/pca.70021","url":null,"abstract":"<p><strong>Introduction: </strong>The role of macromolecular components in traditional Chinese medicines (TCMs) decoction remains poorly understood, primarily because of their structural complexity and limited systemic bioavailability. In the modernization of TCMs' decoctions, macromolecular components are often removed as \"poor bioavailable impurities\" by ethanol precipitation that might attenuate the efficacy thereof.</p><p><strong>Purpose: </strong>A novel strategy integrating LC-MS-based serum pharmacochemistry, network pharmacology, and pharmacodynamics was developed to reveal the role of macromolecular components in TCMs' decoction, using Qi-Huo-Yi-Fei decoction (QH) treating chronic obstructive pulmonary disease (COPD) as a case study.</p><p><strong>Materials and methods: </strong>QH was separated into macromolecular components (QH-M) and small molecular components (QH-S) by ethanol precipitation. The absorbed components of QH and QH-S in serum were qualitatively and semiquantitatively analyzed by UPLC-QTOF-MS/MS. The differences in targets and pathways of the absorbed components were predicted by network pharmacology analysis. The therapeutic effects on pulmonary function, histopathology, and inflammation of QH, QH-S, and QH-M were comparatively investigated on a COPD rat model.</p><p><strong>Result: </strong>A total of 103 components were identified in QH and QH-S, whereas 86 and 72 were detectable in QH- or QH-S-treated serum, respectively. The highest levels of absorbed components appeared from 15 to 60 min for QH-S and at 15 min for QH. The concentrations of most absorbed components of QH were twofold higher than those of QH-S. Their protein-protein interaction (PPI), herb-component-target networks, and GO/KEGG enrichment pathways were quite different, mainly in inflammatory, immunity, and cell apoptosis processes. QH-M improved pulmonary function, histopathology, and inflammatory infiltration, mainly on FVC (p < 0.05), thickness/external diameter (p < 0.01), Wac/Pbm (p < 0.01), TNF-α (p < 0.01), and IL-17 (p < 0.05). Meanwhile, QH was more effective than QH-S, mainly on FEV0.3/FVC, MAN, GC/CC, TNF-α, and IL-17.</p><p><strong>Conclusion: </strong>QH-M was not only effective independently on COPD but also showed integrative effects with the coexisting QH-S in improving their absorption, thus strengthening the component-target interactions, and consequently contributing to the COPD therapeutic effects of QH. Therefore, it was confirmed that QH-M was essential for QH's COPD therapy and should not be removed during the modern preparation development.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144855912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics-Based Comprehensive Exploration of the Bioactive Compound in Saposhnikovia divaricata (Turcz.) Schischk. in Response to Exogenous Nitrogen.","authors":"Hui Zhi, Sen Shi, Yun Guo, Zhonghua Tang","doi":"10.1002/pca.70015","DOIUrl":"https://doi.org/10.1002/pca.70015","url":null,"abstract":"<p><strong>Background: </strong>Saposhnikovia divaricata (Turcz.) Schischk. (S. divaricata), a traditional Chinese medicine, is renowned for its dried roots rich in phenolic compounds, which exhibit significant medicinal values in anti-inflammatory, analgesic, and other therapeutic fields. Cultivating medicinal plants has been validated as an effective strategy to enhance the supply of phenolic-rich raw materials. However, the efficient fertilization strategy of nitrogen-a key nutrient-and its regulatory mechanisms on plant growth and phenolic metabolism in S. divaricata remain unclear.</p><p><strong>Purpose and study design: </strong>This study aimed to explore nitrogen's effects on S. divaricata growth and phenolic accumulation. Field experiments used four nitrogen treatments: no nitrogen fertilization (CK), low nitrogen fertilization (9 g/m^²), medium nitrogen fertilization (18 g/m^²), and high nitrogen fertilization (27 g/m^²). Growth indices and phenolic metabolites were analyzed via UPLC-MS and multivariate statistics.</p><p><strong>Results: </strong>The results showed that the medium nitrogen treatment (18 g/m^²) significantly promoted the growth of S. divaricata, with plant height and single-plant biomass reaching 26 ± 0.3 cm and 19.04 ± 0.96 g, respectively, and optimal root development (root length 14.7 ± 0.2 cm). Additionally, nitrogen application significantly promoted the synthesis of phenolic compounds and enhanced the tolerance of S. divaricata to nitrogen stress, which is conducive to safeguarding and elevating its medicinal value. For specific bioactive substances, the contents of prim-o-glucosyl-cimifugin, 4'-o-β-glucosyl-5-o-methylvisamminol, cimifugin, and sec-o-glucosylhamaudol in S. divaricata under medium nitrogen concentration were notably higher than those in the no-nitrogen fertilization group.</p><p><strong>Conclusion: </strong>This study confirms that medium nitrogen levels can synergistically enhance the growth and accumulation of medicinal active components in S. divaricata, providing a theoretical basis for the precise application of nitrogen fertilizers and quality regulation in medicinal plant cultivation.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144855913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality Analysis of Raw Material and Products of Goat Bezoar (Houzao), a Mysterious Traditional Medicine.","authors":"Sing-Wan Pun, Hau-Yee Fung, Peili Zhu, Zhu Zhang, Hanting Hu, Yinan Feng, Zhongzhen Zhao, Lifeng Li, Quanbin Han","doi":"10.1002/pca.70022","DOIUrl":"https://doi.org/10.1002/pca.70022","url":null,"abstract":"<p><strong>Background: </strong>Goat bezoar (Houzao in Chinese, also called \"monkey\" bezoar or Caprae Calculus) is a valued traditional Chinese medicine (TCM) used in the treatment of pediatric cough for centuries. However, its undefined chemical composition hinders quality control and mechanistic understanding, limiting its modernization and further development.</p><p><strong>Objective: </strong>This study aims to comprehensively characterize the chemical composition of goat bezoar and develop a robust quality control method.</p><p><strong>Method: </strong>UPLC-qTOF-MS was first utilized to analyze the methanol extract of goat bezoar reference material. Markers specific to the medicinal portion were identified by comparing the chemical profiles with the non-medicinal portion and 50 co-formulated TCMs. Amino acid composition and ash content were also determined. Validated quantitative methods for these markers were subsequently developed using both UPLC-qTOF-MS and UPLC-DAD and applied to assess the quality of 13 batches of raw goat bezoar material and 38 batches of commercial products.</p><p><strong>Results: </strong>Eight characteristic markers, including ellagic acid and seven of its gut microbial metabolites (urolithins), were identified exclusively within the medicinal portion. Ellagic acid (72.72%-89.41%) and total urolithins (2.14%-5.13%) were major components. These, along with amino acids (1.19%-5.91%) and total ash (2.09%), account for over 90% of the medicinal portion's composition. The analysis of commercial materials and products using this method revealed that goat bezoar may be absent in 20 commercial products while all the goat bezoar powder samples were authentic.</p><p><strong>Conclusion: </strong>This study provides the first comprehensive chemical characterization of goat bezoar, revealing its unique composition and establishing it as the only known TCM with urolithins. Validated quantitative methods for ellagic acid and urolithins provide a crucial quality control tool, paving the way for further research, development, and modernization of this valuable TCM.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144855914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Herbal Research, Phytochemistry, Pharmacology, Comprehensive Utilization, and Quality Control of Hemp Seed: A Comprehensive Review.","authors":"Lingyang Kong, Shan Jiang, Lengleng Ma, Junbai Ma, Wei Wu, Chenliang Li, Weichao Ren, Jiao Xu, Wei Ma","doi":"10.1002/pca.70014","DOIUrl":"https://doi.org/10.1002/pca.70014","url":null,"abstract":"<p><strong>Background: </strong>Cannabis sativa L. is an annual herbaceous plant in the genus Cannabis of the family Cannabaceae. Hempseed, a traditional Chinese medicine, is the dried and ripe decorticated fruit of C. sativa L. It has a long history of medicinal use and belongs to the category of \"both medicine and food.\" The earliest record of hempseed as food can be found in \"The Book of Rites\" (Zhou Li), while its medicinal use was first documented in Shennong's Classic of Materia Medica (Shennong Bencao Jing). Hempseed contains rich chemical components, such as fatty acids, cannabinoids, lignin amides, alkaloids, flavonoids, and proteins, among which fatty acids are the most abundant. Hempseed has the effects of moistening the intestines and relieving dryness, invigorating the middle-jiao, and replenishing qi. It is often used for blood deficiency and body fluid deficiency, intestinal dryness, and constipation, and is an important drug for the treatment of functional constipation. Modern medicinal chemistry and pharmacological studies have shown that hempseed semen not only has a significant laxative effect but also possesses pharmacological effects such as anti-inflammation, anti-oxidation, anti-tumor, analgesia, lipid-lowering, and others.</p><p><strong>Objectives: </strong>At present, the quality standards for hemp kernels have not yet established content control for single or whole components. This not only cannot fully guarantee the quality and safety of medicinal materials but also restricts the improvement of quality standards. We summarize the knowledge in this area with the aim of providing new ideas for the scientific application of cannabis and the integration of modern research and traditional medicine.</p><p><strong>Methods: </strong>Relevant literature was retrieved from major scientific databases, including PubMed, Web of Science, ScienceDirect, and Google Scholar. The system collected and analyzed information related to the phytochemistry, pharmacological effects, comprehensive utilization, and quality control of hemp seeds.</p><p><strong>Results: </strong>This article provides a review of the research on traditional Chinese medicine Ma Ren from the aspects of herbal research, phytochemistry, and pharmacology, and emphasizes the latest progress in the comprehensive utilization and quality control of hemp seeds.</p><p><strong>Conclusion: </strong>Hemp seeds contain various bioactive compounds with broad pharmacological potential. They have shown potential in disease management and prevention and are increasingly being applied in the development of functional health products. At the same time, in the future, we need to strengthen interdisciplinary mechanism analysis, accelerate clinical validation, optimize intelligent manufacturing technology, build a standard system for the entire industry chain, and promote the leapfrog development of hemp seeds from functional raw materials to precision and healthy products.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144837300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guided Discovery of Antidiabetic Sesquiterpenoids From Lindera Aggregate by Activity Labeled Molecular Networking.","authors":"Jinqian Yu, Lei Zhao, Galabada Arachchige Sirimal Premakumara, Yingjian Guo, Tao Yue, Sagdullaev Shamansur Shaksaidovich, Xiao Wang","doi":"10.1002/pca.70017","DOIUrl":"https://doi.org/10.1002/pca.70017","url":null,"abstract":"<p><strong>Introduction: </strong>The roots of Lindera aggregata (Sims) Kosterm named \"Wuyao\" in Chinese, showed significant antidiabetic bioactivity detected by the insulin resistance HepG2 cells. However, efficient identification of the new antidiabetic compounds has been lacking.</p><p><strong>Objective: </strong>To develop a guided discovery strategy for new antidiabetic compounds from L. aggregate with activity labeled molecular networking (ALMN).</p><p><strong>Methodology: </strong>The constructed ALMN could clearly visualize the activities and the structure nodes, which can facilitate the annotation of the active candidates.</p><p><strong>Results: </strong>Eight new sesquiterpenoids (1-8) were obtained from the roots of Lindera aggregate according to the ALMN workflow showed antidiabetic activity. As for the new compounds, 1 was characteristic of 15-nor-lindenane type, which was reported for the first time, 2 and 3 of aconitane type, 4 of germarane type, 5-8 of eudesmane type sesquiterpenes.</p><p><strong>Conclusion: </strong>As results indicated, all the eight compounds showed significant antidiabetic effects, which were consistent with the obtained activity annotations by ALMN. The mechanism of the antidiabetic activity for 1-8 were further delved via Akt/GSK3β signal pathway, which can be observed as potential agents against the insulin resistance for the insulin resistance Type II diabetes.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhododendri Daurici Folium: An Overview of Studies on the Study of Herbal Medicine, Chemical Composition, Pharmacological Action, Clinical Application, and Quality Control.","authors":"Chenliang Li, Huixuan Tian, Wei Wu, Lingyang Kong, Jiao Xu, Lihong Zhang","doi":"10.1002/pca.3537","DOIUrl":"10.1002/pca.3537","url":null,"abstract":"<p><p>The Rhododendri Daurici Folium (RDF) represents the desiccated foliage of Rhododendron dauricum L., belonging to the Rhododendron family. RDF abounds in diverse chemical elements, predominantly flavonoids, volatile oils, phenols, coumarin, and lignans. RDF functions as a cough and expectorant agent, primarily employed in the management of acute and chronic bronchitis and pharyngitis. RDF is also known for its pharmacological properties, including antibacterial, cardiotonic, antihypertensive, and anticancer properties. RDF is frequently utilized as a potent ingredient in Yingshanhong tablets, compound RDF syrup, XiaoKeChuan syrup, and various traditional Chinese medicinal concoctions, and it holds significant importance in clinical settings. This article's data were sourced from publications including PubMed, CNCK, SCI-HUB, Web of Science, Scopus, Chinese Pharmacopoeia, Chinese Dictionary of Traditional Chinese Medicine, Chinese Flora, and associated studies. The present document provides a comprehensive examination of the herbal properties, pharmacological effects, chemical makeup, clinical application, quality assurance, and safety assessments of RDF, serving as a guide for advancing and applying traditional Chinese medicine RDF.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"1649-1676"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}