Phytochemical Analysis最新文献

{"title":"Comparative Metabolic Profiling of Wild and In Vitro Cultivated Cecropia angustifolia Roots Fraction Reveals Conservation of Pentacyclic Triterpene Acid Biosynthesis.","authors":"Luis M Díaz-Sánchez, Martha L Chacón-Patiño, Cristian Blanco-Tirado, Marianny Y Combariza, Guillermo Montoya","doi":"10.1002/pca.70001","DOIUrl":"10.1002/pca.70001","url":null,"abstract":"<p><strong>Introduction: </strong>Cecropia angustifolia roots are a traditional source of pentacyclic triterpene acids (PTAs), bioactive compounds with promising therapeutic potential. In vitro cultivation offers a sustainable alternative to metabolite production while minimizing the impact on wild populations.</p><p><strong>Objective: </strong>To compare the metabolic profiles of wild and in vitro cultivated C. angustifolia roots using ultrahigh-resolution mass spectrometry.</p><p><strong>Methods: </strong>Root fractions from wild and in vitro cultivated materials were analyzed using negative-ion electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI(-)-FT-ICR-MS) at 21 Tesla.</p><p><strong>Results: </strong>Molecular analysis revealed diverse PTA species in both wild and in vitro root fractions. While in vitro cultivation produced a more streamlined metabolic profile, PTA content remained significantly enriched.</p><p><strong>Conclusion: </strong>In vitro cultivation of C. angustifolia roots represents a viable strategy for sustainably producing pharmacologically relevant PTAs, supporting both conservation and the biotechnological use of natural products.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"1829-1839"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Inflammatory Saponins From an Edible Aquatic Plant Nymphoides hydrophylla.","authors":"Ching-Yeu Chen, Tzu-Peng Cheng, Tsong-Long Hwang, Yong-Siang Hou, Limbadri Salendra, Tzi-Yi Ke, Yuan-Bin Cheng","doi":"10.1002/pca.3539","DOIUrl":"10.1002/pca.3539","url":null,"abstract":"<p><strong>Introduction: </strong>The present investigation of Nymphoides hydrophylla is a Taiwan indigenous edible aquatic plant, belongs to the Menyanthaceae family, and has been used as a traditional food with potential medicinal values against fever, insect bites, ulcer, and skin diseases. Interestingly, we found that the plant extract showed a significant anti-inflammatory effect.</p><p><strong>Objectives: </strong>The aim is isolation, structure elucidation, and anti-inflammatory evaluation of secondary metabolites from N. hydrophylla.</p><p><strong>Material and methods: </strong>This study involves the bioactive isolation and structural studies of the pure compounds determined by NMR, UV, IR spectroscopies, and HRMS. The anti-inflammatory activity of the pure compounds was evaluated in vitro. In addition, isolates showed remarkable anti-inflammatory activities to regulate superoxide anion generation and elastase release in fMLF/CB-induced human neutrophils.</p><p><strong>Results: </strong>This study conducted 10 oleanane-type saponins with oligoglycoside linkage, including two new (1 and 2) identified from the butanol extract of N. hydrophylla. As a result, Compounds 1 and 2, representing new triterpene saponins, have not been previously reported. In vitro anti-inflammatory tests revealed that Compounds 1, 5-7, 9, and 10 showed significant anti-inflammatory effects by inhibiting superoxide anion generation with IC₅₀ values of 1.62, 0.92, 0.28, 2.69, 0.29, and 0.15 μM, respectively, whereas compounds 1, 5, 6, 9, and 10 showed potential inhibition on elastase release in human neutrophils, with IC₅₀ values of 0.19, 1.12, 0.70, 0.68, and 0.51 μM, respectively.</p><p><strong>Conclusion: </strong>Our findings suggested that the components of N. hydrophylla have the potential to develop anti-inflammatory agents.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"1710-1718"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12354994/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality Analysis of Curcumae Radix From Different Sources Based on the Three-Dimensional Evaluation System of \"Morphology-Composition-Efficacy\".","authors":"Yv Wang, Zhewen Zhang, Liu Yang, Pei Ye, Yv Ye, Xiaoning Li, Weifeng Du","doi":"10.1002/pca.3541","DOIUrl":"10.1002/pca.3541","url":null,"abstract":"<p><strong>Introduction: </strong>A major limitation in current Traditional Chinese Medicine (TCM) research lies in its overreliance on single-factor analyses of chemical composition or pharmacodynamics, or simplistic combined models. While these approaches partially elucidate TCM characteristics, they fail to comprehensively capture its holistic complexity and inherent quality.</p><p><strong>Objectives: </strong>The three-dimensional comprehensive evaluation method of \"morphology-composition-efficacy (MCE)\" was creatively introduced in this study, and the quality of four different varieties of Curcumae Radix was deeply and meticulously studied.</p><p><strong>Materials and methods: </strong>Curcumae Radix from different sources was collected, and its external morphology (volume and weight, color and smell), internal composition (extract, fingerprint and literature knowledge network diagram) and drug effect (efficacy of zebrafish on improving qi stagnation and blood stasis) were analyzed. The differences in composition and morphology of four kinds of Curcumae Radix were studied by HPLC fingerprint, chromaticity identification model, and BP-ANN classification model. Secondly, through the experiment of zebrafish \"qi stagnation and blood stasis\" model, the improvement effect of four kinds of turmeric on \"qi stagnation and blood stasis\" was explored. Finally, the relationship between the three dimensions of MCE of Curcumae Radix was discussed through Pearson analysis.</p><p><strong>Results: </strong>The pharmacodynamic experiment of zebrafish confirmed that all four kinds of Curcumae Radix showed the activity of treating \"qi stagnation and blood stasis\" and Curcuma phaeocaulis Val. (LSYJ) had the best effect comprehensively. And through Pearson analysis, it can be concluded that the smaller the external volume and weight of Curcumae Radix, the stronger the characteristic odor (W5S, W1W, and W2W), the closer the powder color is to red and yellow, the higher the internal extract (JCW) content of Curcumae Radix, the higher the contents of germacrone (JMT), furanodiene (FNEX), and β-elemene (β-LXX), and the better the efficacy of improving the disease of \"qi stagnation and blood stasis,\" among which JMT is the key drug component.</p><p><strong>Conclusion: </strong>The research demonstrates the value of a three-dimensional evaluation system for TCM quality assessment, providing insights into the relationship between morphology, composition, and efficacy. It offers a new perspective for quality control and evaluation of Curcumae Radix and provides a scientific basis for the clinical treatment of \"qi stagnation and blood stasis\" related diseases.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"1747-1765"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144192065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Advances in Analytical Methods for Aconitine Alkaloid in Natural Products: A Systematic Review.","authors":"Latif Ahmad, Minxia Fan, Juhar Zemede, Andrew J Semotiuk, Guilin Chen, Guangwan Hu","doi":"10.1002/pca.70003","DOIUrl":"10.1002/pca.70003","url":null,"abstract":"<p><strong>Introduction: </strong>Aconitine, the main toxic and active compound in Aconitum species, is a key component of Traditional Chinese Medicine (TCM), valued for its anti-inflammatory, cardiotonic, and analgesic properties. However, its potent neurotoxicity and cardiotoxicity, potentially leading to fatal outcomes, necessitate careful evaluation and management.</p><p><strong>Objectives: </strong>Accurately identifying aconitine in natural products is one of the hot issues of international concern regarding the safety of TCM. This review systematically evaluates the current scientific literature to assess advancements in analytical methodologies for identifying and quantifying aconitine.</p><p><strong>Methods: </strong>This review systematically evaluates literature from 2000 to 2025 regarding analytical methods for aconitine determination, utilizing databases such as PubMed, ScienceDirect, and Google Scholar.</p><p><strong>Results: </strong>From 2237 identified articles, 90 were evaluated. This review highlights the advantages of LC-MS, DESI-MSI, and ambient MS techniques for identifying and quantifying aconitine, emphasizing their rapid analysis, minimal sample preparation, and cost-effectiveness. These advanced methods offer significant potential for reliable applications in various fields. DESI-MSI and DART-MS enable solvent-free, rapid analysis in native conditions, whereas oEESI-MS offers high throughput, precise quantification, and reduced solvent/sample use. The modified methods, H-oEESI-MS, allow direct analysis of Aconitum herbal materials without pretreatment, demonstrating high efficiency.</p><p><strong>Conclusions: </strong>This review identifies research gaps and future directions using advanced analytical methods for aconitine determination, aiming to enhance quality control, regulatory compliance, and product safety. The integration of artificial intelligence into aconitine detection and analysis in TCM presents transformative potential, promising enhanced accuracy, efficiency, and safety while stimulating innovation and guiding future research directions in analytical science.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"1621-1648"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144541890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical Recognition and Spectrum-Effect Relationship of UPLC-MS Chromatograms With Anti-Complementary and Antioxidant Activities of Myricariae Ramulus.","authors":"Mengyao Zhang, Deji, Daofeng Chen, Yan Lu","doi":"10.1002/pca.3527","DOIUrl":"10.1002/pca.3527","url":null,"abstract":"<p><strong>Introduction: </strong>Myricariae Ramulus (MR) is a traditional anti-inflammatory Tibetan medicine derived from the branches and leafy twigs of various Myricaria plants, such as Myricaria wardii Marquand.</p><p><strong>Objective: </strong>This study performed spectrum-effect analyses on 15 batches of MR, sourced from various origins and medicinal parts, to identify quality markers associated with its anti-inflammatory effects.</p><p><strong>Materials and methods: </strong>The anti-inflammatory effects of different extracts and fractions from M. wardii were evaluated in lipopolysaccharide-infected mice. Further analysis was conducted utilizing UPLC-Q-TOF-MS/MS in the negative ion mode to recognize the compound composition of ethyl acetate (EA) fractions from the branches and leafy twigs of M. wardii. Subsequently, anti-complementary and antioxidant activities were conducted via the hemolysis and ABTS radical scavenging methods, and UPLC chromatograms for 15 batches of samples were established. Finally, spectrum-effect analysis was performed by gray relational analysis and partial least squares regression to identify the quality markers.</p><p><strong>Results: </strong>The EA fraction effectively alleviated inflammatory responses by regulating IL-6 and myeloperoxidase. A total of 46 compounds from M. wardii were recognized, which mainly encompass gallic derivatives, phenolic amides, flavonoids, and terpenoids. Activity assays demonstrated that the two activities of leafy twigs were significantly stronger than those of branches. Moreover, an obvious distinction in chemical composition between the two parts was observed. Combining the spectrum-effect analysis results, methyl 3,4-dihydroxy-5-methoxybenzoate, myricarin A, protocatechualdehyde, and N-feruloyl normetanephrine from the branches, and protocatechuic acid from the leafy twigs were considered quality markers.</p><p><strong>Conclusion: </strong>These results provide insights into improving the quality control of MR.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"1502-1516"},"PeriodicalIF":3.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Antibacterial Agents with a Synergistic Effect in Pteris vittata L. by Exploration of Metabolome Data and Multivariate Analysis.","authors":"Kieu-Oanh Nguyen Thi, Ngoc-Lien Nguyen, Khanh-Phuong Dang, Phuong-Nhung Nguyen, Huyen-Thu Nguyen Thi, Hoang-Nam Pham, Sylvie Nazaret, Marie-Geneviève Dijoux-Franca","doi":"10.1002/pca.3530","DOIUrl":"10.1002/pca.3530","url":null,"abstract":"<p><strong>Introduction: </strong>In metalliferous ecosystems, bacteria could develop membrane efflux pumps to extrude heavy metals. Native plants, frequently exposed to such bacteria, may serve as rich chemical reservoirs for discovering potential antibacterial and/or resistance-modifying agents.</p><p><strong>Objectives: </strong>This study aims to investigate the antibacterial properties of Pteris vittata L., a metal hyperaccumulator abundant in metal-rich environments, against Stenotrophomonas maltophilia K279a, an opportunistic nosocomial pathogen linked to severe respiratory tract infections.</p><p><strong>Material and methods: </strong>A novel approach was used, employing the metabolome of P. vittata in a partial least squares regression model to predict the correlation between the whole chemical profile and activity. Marked compounds were then subjected to in vitro test for bactericidal and synergistic properties using broth dilution and time-kill assays.</p><p><strong>Results: </strong>The multivariate analysis demonstrated a strong synergistic effect among a range of flavonoids compared to the individual responses. Notably, nine flavonoids were confirmed to reduce the minimum inhibitory concentration and minimal bactericidal concentration values of active compounds, cyanidin-3,5-O-diglucoside and perlagonin, from 64.0 and 108 μg/mL to 0.25 and 3.38 μg/mL, respectively.</p><p><strong>Conclusion: </strong>These results provide insights into the possibility of combating clinical infections caused by S. maltophilia and highlight the potential of exploring new synergetic interactions among natural products as an alternative to the conventional bio-guided isolation strategy.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"1560-1569"},"PeriodicalIF":3.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality Grading Evaluation of Changii Radix Based on a Biology-Related Chemical Indicator Prediction Model.","authors":"Ke Liu, Chun-Lu Liu, Long Wang, Ping Li, Yan Jiang, Hui-Jun Li","doi":"10.1002/pca.3524","DOIUrl":"10.1002/pca.3524","url":null,"abstract":"<p><strong>Introduction: </strong>The quality of traditional Chinese medicine (TCM) is a prerequisite for clinical efficacy. However, the existing quality evaluation methods are not strongly correlated with efficacy, and they are unable to adequately reflect the quality grade of Changii Radix (CR).</p><p><strong>Objectives: </strong>In this study, a biology-related chemical indicator quality grading prediction model was developed to predict the quality grade of CR.</p><p><strong>Materials and methods: </strong>Firstly, the quality grade of CR was pre-classified based on immunological activity. Subsequently, one-way analysis of variance, gray correlation analysis, and Pearson correlation analysis were employed to identify the chemical indicators associated with immunological activity. Finally, separately using chemical indicators as independent variables and quality grades as dependent variables, the logistic regression model and a multi-index weighted quality comprehensive evaluation index (QCEI) were constructed to predict the quality grade of CR.</p><p><strong>Results: </strong>The results indicated that 27 batches of CR samples could be divided into three grades of I, II, and III. The gray correlation degrees and Pearson correlation coefficients between water-soluble extractives, polysaccharide, amino acid, and immunological activity all exceeded 0.8 and 0.4 (p < 0.05), respectively. Additionally, both the logistic regression model and QCEI could effectively predict the quality grade of CR, with the logistic regression model showing superior performance.</p><p><strong>Conclusion: </strong>This study is the first to establish a chemistry-biology integrated strategy for evaluating the quality grade of CR, providing a novel insight into the assessment of TCM quality grade.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"1490-1501"},"PeriodicalIF":3.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted Isolation of Coumarins From Sideritis Species Based on Antiviral Screening and Untargeted Metabolomics.","authors":"Ekaterina-Michaela Tomou, Olivier Engler, Antonios Chrysargyris, Nikolaos Tzortzakis, Helen Skaltsa, Corinna Urmann","doi":"10.1002/pca.3531","DOIUrl":"10.1002/pca.3531","url":null,"abstract":"<p><strong>Introduction: </strong>The SARS-CoV-2 pandemic has revealed a deficiency in antiviral agents. Plants, traditionally used for respiratory infections, are valuable sources of antiviral compounds. Such a plant is the Sideritis L. taxa (mountain tea), traditionally used against cold and cough.</p><p><strong>Objectives: </strong>Accordingly, this study aimed to investigate the potential protective effects of dichloromethane extracts from Sideritis species against SARS-CoV-2.</p><p><strong>Materials and methods: </strong>Eight Sideritis extracts were tested in an in vitro pretreatment assay to assess the protective effect against SARS-CoV-2. Therefore, infectious virus particles were pre-incubated with the extract, then incubated with Vero E6 cells to finally measure cell viability as a surrogate for virus infection. Untargeted analyses (GC-MS and LC-PDA-HRESIMS) were performed to determine metabolite profiles.</p><p><strong>Results: </strong>Using an orthogonal approach that combines untargeted metabolomics and biological data from a screening assay, we characterized the phytochemical profiles of the different extracts and prioritized samples for targeted isolation. The dichloromethane extract of Sideritis cypria exhibited a notable protective effect. Untargeted analysis revealed coumarins as key compounds, with varying amounts across Sideritis species. Accordingly, fractionation of extract resulted in the isolation of two coumarin derivatives. Structure elucidation was performed using one- and two-dimensional nuclear magnetic resonance experiments. The coumarin, more abundant in S. cypria, demonstrated a slight protective effect in the SARS-CoV-2 pretreatment assay.</p><p><strong>Conclusion: </strong>This study highlights the antiviral effects of Sideritis taxa, although further investigations are necessary to clarify the full potential of the herb. Additionally, the methodology presented herein can serve as a valuable resource for future phytochemical investigations focused on coumarin content within Sideritis genus.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"1570-1579"},"PeriodicalIF":2.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12212021/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and Identification of the Chemical Constituents and the Metabolites of Geum japonicum Thunb. var. chinense F. Bolle.","authors":"Yang Yang, Tong Wu, Leilei Jiang, Lixia Wang, Xiaoxu Chen, Tianxin Zhang, Zhuju Wang, Ye Zhao, KaiYang Liu, Hongwei Wu, Liying Tang","doi":"10.1002/pca.3528","DOIUrl":"10.1002/pca.3528","url":null,"abstract":"<p><strong>Introduction: </strong>Geum japonicum Thunb. var. chinense F. Bolle (GJ) holds significance as a traditional Miao medicine, one of China's ethnic minorities, extensively utilized in the clinical management of cardiovascular ailments. However, little attention has been directed toward the chemical constituents of GJ, as well as the metabolic and distribution of its chemical constituents in vivo.</p><p><strong>Objective: </strong>The objective of this study is to qualitatively analyze the chemical constituents of GJ, as well as their prototype components and metabolites in vivo.</p><p><strong>Methods: </strong>We established a reliable method using ultra-high-performance liquid chromatography coupled with quadrupole Exactive Orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS) to conduct a comprehensive analysis of GJ as well as systematically to screen the prototype and metabolite constituents in rat plasma, urine, feces, brain, heart, liver, spleen, and kidney after gastric perfusion.</p><p><strong>Results: </strong>A total of 94 compounds were identified or tentatively characterized in GJ. Moreover, a total of 75 in vivo components were identified from the plasma, urine, feces, brain, heart, liver, spleen, and kidney of rats gavaged with 500 mg/kg of GJ extract, including 19 prototype components and 56 metabolites. The metabolic pathways, including Phase I reactions and Phase II reactions, for the absorbed constituents, were explored and summarized. Moreover, the metabolic pathways of GJ were proposed, which shed light on their mechanism.</p><p><strong>Conclusion: </strong>This is the first systematic study on the components of GJ and their metabolites in vivo and in vitro. The metabolite distribution and metabolic pathways of GJ in vivo were further proposed.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"1517-1538"},"PeriodicalIF":3.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Extraction Process, Structural Characterization, and Antioxidant and Hypoglycemic Activity Evaluation of Polysaccharides From the Medicinal and Edible Plant: Cistanche deserticola Ma.","authors":"Tao-Tao Xue, Dong-Xuan Zheng, Qiang Hou, Li-Mei Wen, Bao-Juan Wang, Ruo-Yu Geng, Qian-Qian Wang, Wu Dai, Li-Ying Tian, Sheng-Qi He, Jian-Hua Yang, Jun-Ping Hu","doi":"10.1002/pca.3512","DOIUrl":"10.1002/pca.3512","url":null,"abstract":"<p><strong>Introduction: </strong>Cistanche deserticola Ma (CD), an edible and medicinal plant native to Xinjiang, Inner Mongolia, and Gansu in China, is rich in bioactive polysaccharides known for their health-promoting properties. The polysaccharides of C. deserticola (CDPs) have been shown to possess a range of beneficial activities, including immunomodulatory, anti-aging, antioxidant, and anti-osteoporosis effects.</p><p><strong>Objective: </strong>This study seeks to identify the optimal conditions for extracting CDPs using hot water. Additionally, it aims to evaluate their chemical properties, antioxidant activity, hypoglycemic effects, and cytotoxicity. The findings will provide a theoretical foundation for the potential use of CDPs in functional foods and pharmaceuticals.</p><p><strong>Methodology: </strong>The study employed response surface methodology to optimize the hot water extraction conditions for CDPs. The extracted CDPs were characterized using a range of chemical, spectroscopic, and instrumental analyses. Furthermore, their antioxidant activity, hypoglycemic effects, and cytotoxicity were evaluated through relevant assays to assess their potential health benefits.</p><p><strong>Results: </strong>Under optimal conditions, the yield of CDPs was 45.85% ± 1.91%. CDPs were identified as acidic heteropolysaccharides with a wide molecular weight distribution, ranging from 0.3 to 128.2 kDa. They were composed primarily of glucose (51.21%), arabinose (32.86%), galactose (17.88%), and smaller amounts of galacturonic acid (4.66%), rhamnose (1.85%), mannose (1.32%), glucosamine hydrochloride (1.08%), and xylose (0.56%). Antioxidant assays demonstrated that CDPs exhibited significant free radical scavenging activity, metal ion chelation, and reducing power. Additionally, CDPs inhibited α-glucosidase and α-amylase in vitro through a mixed-type mechanism, as well as static fluorescence quenching. Cytotoxicity assays showed that CDPs were nontoxic to L02 and AML12 cells.</p><p><strong>Conclusion: </strong>This study offers a theoretical foundation for the potential use of CDPs in functional foods and pharmaceuticals and provides valuable insights for the development of new antioxidant and hypoglycemic agents from natural sources.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"1333-1350"},"PeriodicalIF":3.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143033404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}