Phytochemical Analysis最新文献

{"title":"Identification of Schisandra chinensis Seeds and Exclusion of Common Adulterants by Allele-Specific PCR Based on ITS2 Sequences.","authors":"Hui Tian, Yidi Yang, Chi Ma, Xiuna Zhang, Jing Fang, Fanna Qu, Lihong Yang","doi":"10.1002/pca.70031","DOIUrl":"10.1002/pca.70031","url":null,"abstract":"<p><strong>Background: </strong>In terms of medicinal material market and seed sources, Schisandra sphenanthera Rehd.et Wils. is often misused as Schisandra chinensis (Turcz.) Baill. Although the Chinese Pharmacopoeia makes a distinction at the medicinal material level, there is no reliable identification method for seeds. The existing approach still relies on traditional morphology, which requires rich experience.</p><p><strong>Objectives: </strong>This study aims to fill this gap and enable quality control at the source level.</p><p><strong>Material and methods: </strong>A neighbor-joining (NJ) tree based on the ITS2 gene was constructed to analyze species genetic relationships and find SNP site for S. chinensis. Primers targeting the SNP site were used in an allele-specific PCR system, whose sensitivity was tested with different amounts of DNA and further validated by commercial samples.</p><p><strong>Results: </strong>The NJ tree showed that S. chinensis clustered into a single strand, clearly separated from other related species. When the primers were applied to the allele-specific PCR, a diagnostic 273 bp band was amplified specifically in S. chinensis seeds, with no cross-reactivity observed. This assay system exhibited high sensitivity, detecting as little as 0.5 ng of genomic DNA, and was validated by commercial samples, suggesting its accuracy, reproducibility, and practical applicability.</p><p><strong>Conclusion: </strong>This method is an ideal tool for large-scale seed authentication of S. chinensis due to its high efficiency, precision, and operational simplicity. Our research contributes to quality control throughout the supply chain by ensuring the purity of the seeds at the earliest stages of cultivation, and also promotes the standardized production of this valuable medicinal resource.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"117-126"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145150447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Tianma Anshen Dingxuan Formula Regulates Gut Microbiota and Metabolites in a Rat Model of Vertigo With Liver Yang Hyperactivity.","authors":"Xinghu Fan, Huiying Li, Jianxuan Liu, Yanling Lin, Li Sun, Bo Liu, Wei Zhao, Yingxia Yu, Hongyan Zhang, Moyan Wang, Xin Teng, Zhen Yang","doi":"10.1002/pca.70030","DOIUrl":"10.1002/pca.70030","url":null,"abstract":"<p><strong>Introduction: </strong>Vertigo is a prevalent clinical disorder that significantly compromises the quality of life of a patient. Among the syndrome patterns recognized in traditional Chinese medicine (TCM), vertigo due to liver-yang hyperactivity (LYH) is the most common subtype. This study explored the underlying therapeutic mechanisms of Tianma Anshen Dingxuan formula (TADF) on vertigo induced by LYH through the analysis of gut microbiota and their metabolites.</p><p><strong>Methods: </strong>A total of 30 Sprague-Dawley rats were assigned to control, LYH vertigo model, and TADF groups. The LYH vertigo model was established through intragastric administration of aconite decoction combined with unilateral labyrinthectomy. Behavioral changes and colonic histopathology were conducted. The concentrations of 5-hydroxytryptamine (5-HT), dopamine (DA), and norepinephrine (NE) in the rat hypothalamus were quantified using the enzyme-linked immunosorbent assay (ELISA). The gut microbiota and metabolites were analyzed using 16S rRNA gene sequencing and ultrahigh-performance liquid chromatography-mass spectrometry (UHPLC-MS). Spearman correlation analysis was performed to explore the relationships among the microbiota, metabolites, and behavioral indices.</p><p><strong>Results: </strong>Treatment with TADF significantly improved behavioral indices (such as reduced head tilt, normalized reflexes) and colonic inflammation (lower histopathological scores, p < 0.01), whereas it significantly reduced the concentrations of 5-HT, DA, and NE in the rat hypothalamus (p < 0.05). Additionally, TADF modulated gut microbiota by upregulating both p_Patescibacteria and o_Oscillospirales while downregulating g_Bifidobacterium. Metabolomics identified 16 biomarkers (for instance, N-acetylneuraminate) enriched in pathways like tryptophan metabolism. Spearman analysis revealed correlations between microbiota (g_Ruminococcus), metabolites (chlorphenoxamine), and behavioral scores.</p><p><strong>Conclusion: </strong>Collectively, this study is the first to demonstrate the therapeutic effect of TADF on vertigo-afflicted rats. TADF significantly improved the levels of microbiota-mediated metabolites by restoring the impaired gut microbiota.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"100-116"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HR-LC/MS Guided Separation and Identification of Acyl-Migrated Sucrose Esters From Physalis Calyx seu Fructus.","authors":"Chuanyang Zhang, Lujia Yang, Lishi Jiang, Yuting Mu, Xianglian Mao, Qian Liu, Fang Deng, Dale Guo","doi":"10.1002/pca.70028","DOIUrl":"10.1002/pca.70028","url":null,"abstract":"<p><strong>Introduction: </strong>Physalis Calyx seu Fructus is a commonly used traditional Chinese medicine with recognized effects of clearing heat, detoxifying, soothing the throat, and resolving phlegm. Previous studies have identified it as a rich source of sucrose fatty acid esters (SEs).</p><p><strong>Objectives: </strong>The aim of this study was to isolate structurally novel SEs with biological activity.</p><p><strong>Material and methods: </strong>HR-LC/MS guided isolation was conducted, followed by unequivocal structural characterization of all compounds through comprehensive spectroscopic analysis. All compounds were evaluated for anti-inflammatory activity using an LPS-stimulated RAW 264.7 macrophage model, and their antibacterial activity was assessed against three clinically relevant bacterial strains. Simultaneously, isotope labeling experiments were conducted to elucidate the role of H₂O in the acyl migration process of the SEs.</p><p><strong>Results: </strong>Ten new SEs, designated Phyalkfranose A-J (1-10), were isolated; six of them were unstable and underwent intramolecular acyl migration. Experimental results demonstrated that these 6 SEs underwent slow intramolecular acyl migration in aqueous solution, a process that was significantly accelerated under conditions of elevated temperature and high water content. Isotope labeling studies confirmed that H₂O facilitates, but does not participate in the migration. Compounds 8 and 10 inhibited the production of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α). All compounds exhibited negligible antimicrobial activity.</p><p><strong>Conclusion: </strong>This study represents the first report on the acyl migration phenomenon of naturally occurring SEs from the plant source. These findings not only enrich the structural diversity of SEs but also offer novel strategies and methodologies for the isolation of such components.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"85-99"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145070537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted Submetabolome Profiling of Bufadienolides in Venenum Bufonis Using Scheduled Diagnostic Product Ion-Pair Filtering and Substructure Recognition-Based Strategy.","authors":"Xuelin Sun, Wei Ding, Zhanpeng Shang, Jiantao Qiu, Xueying Tan","doi":"10.1002/pca.70025","DOIUrl":"10.1002/pca.70025","url":null,"abstract":"<p><strong>Introduction: </strong>Venenum bufonis (VB), a traditional animal-derived medicine, exhibits significant antitumor, immunomodulatory, and analgesic activities that are primarily attributed to bufadienolides.</p><p><strong>Objective: </strong>This study presented a novel targeted submetabolomics strategy for comprehensive profiling of bufadienolides in VB.</p><p><strong>Material and methods: </strong>This targeted submetabolome mainly integrated scheduled diagnostic product ion (DPI) pair-based filtering and substructure recognition-based structural assembly based on the LC-MS platform. Key steps included 1) stepwise multiple ion monitoring (MIM) coupled with a dynamic exclusion function to enhance detection of minor peaks; 2) automated annotation of unconjugated bufadienolides via high-resolution extracted ion chromatograms (EICs) and a Microsoft Excel-based platform; 3) establishment of an extended tail database for conjugated bufadienolides (including dicarboxylic acid and dicarboxylic acid-amino acid conjugates) based on structural similarity and derivativity, enabling untargeted screening via validated or scheduled DPI pairs derived from MS/MS fragmentation patterns; and 4) potential conjugated bufadienolides were characterized via a substructure recognition-based strategy.</p><p><strong>Results: </strong>The strategy identified 162 bufadienolides (39 primary, 39 secondary, and 84 tertiary) with a 96% annotation rate (155 characterized). Notably, 81 potential novel compounds were discovered, including 11 previously undescribed structural subclasses.</p><p><strong>Conclusions: </strong>This targeted submetabolome strategy significantly expanded the diversity of bufadienolides in VB and also advanced the application of mass spectrometry in natural product discovery.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"40-51"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144964934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Treatment Conditions on the Chemical Ingredient Contents and Activities of Eleutherococcus senticosus Leaves.","authors":"Shuang Jiang, Zhiwei Zhang, Ying Zhang, Xu Jia, Xiaoqiang Chen","doi":"10.1002/pca.70033","DOIUrl":"10.1002/pca.70033","url":null,"abstract":"<p><strong>Background: </strong>Eleutherococcus senticosus leaves (ESL), recognized for their rich phytochemical profile and dual medicinal-nutritional value, hold significant potential as sustainable functional ingredients. However, the impact of post-harvest processing conditions on the retention of their bioactive components and corresponding biological activities remains to be systematically elucidated.</p><p><strong>Purpose and study design: </strong>This study aimed to systematically evaluate the effects of different post-harvest processing methods on the quality of ESL. An integrated approach combining HPLC fingerprinting, chemometric analysis, and multi-activity evaluation (including antioxidant, antimicrobial, enzyme-inhibitory, and cellular activity assays) was employed to clarify how treatment conditions influence ingredient retention and bioactivity in ESL.</p><p><strong>Results: </strong>The findings identified nine chemical markers in ESL, establishing a foundation for quality assessment. Moreover, the combination of vacuum freeze-drying with heated extraction (VD2) most effectively preserved key chemical markers and enhanced antioxidant capacity. This treatment also markedly promoted glutathione (GSH) biosynthesis in astrocytes under oxidative stress, showing a 77% increase compared to the control. In contrast, the VD1 treatment exhibited the strongest inhibitory activity against α-amylase and pancreatic lipase (IC₅₀ values of 0.83 ± 0.056 mg/mL and 1.61 ± 0.057 mg/mL, respectively), while the HD4 treatment showed remarkable potency in inhibiting α-glucosidase (IC₅₀ value of 0.14 ± 0.038 mg/mL).</p><p><strong>Conclusions: </strong>This study demonstrates that specific drying and extraction protocols can selectively enhance desired bioactive properties in ESL. These findings provide a theoretical basis for optimizing industrial-scale ESL production processes, facilitating the targeted development of ESL-based nutraceuticals, neuroprotective adjuvants, and antioxidants.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"140-149"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145452675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolome classification of olive by-products from different oil presses providing insights into its potential health benefits and valorization as analyzed via multiplex MS-based techniques coupled to chemometrics.","authors":"Nesrin M Fayek, Mostafa H Baky, Zhenhao Li, Ibrahim Khalifa, Esra Capanoglu, Mohamed A Farag","doi":"10.1002/pca.3385","DOIUrl":"10.1002/pca.3385","url":null,"abstract":"<p><strong>Introduction: </strong>The Olive (Olea europaea L.) is one of the most popular edible oil-producing fruits, consumed worldwide for its myriad nutritional and health benefits. Olive oil production generates huge quantities of by-products from the fruit, which are considered environmental hazards. Recently, more and more efforts have been made to valorize olive by-products as a source of low-cost, value-added food applications.</p><p><strong>Objective: </strong>The main objective of this study was to globally assess the metabolome of olive fruit by-products, including olive mill wastewater, olive pomace, and olive seeds from fruits from two areas, Siwa and Anshas, Egypt.</p><p><strong>Methods: </strong>Gas chromatography-mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography with mass spectrometry (UPLC-MS) were used for profiling primary and secondary metabolites in olive by-products. Also, multivariate data analyses were used to assess variations between olive by-product samples.</p><p><strong>Results: </strong>A total of 103 primary metabolites and 105 secondary metabolites were identified by GC-MS and UPLC-MS, respectively. Fatty acids amounted to a major class in the olive by-products at 53-91%, with oleic acid dominating, especially in the pomace of Siwa. Mill wastewater was discriminated from other by-products by the presence of phenolics mainly tyrosol, hydroxyl tyrosol, and α-tocopherol as analyzed by UPLC-MS indicating their potential antioxidant activity. Pomace and seeds were rich in fatty acids/esters and hydroxy fatty acids and not readily distinguishable from each other.</p><p><strong>Conclusion: </strong>The current work discusses the metabolome profile of olive waste products for valorization purposes. Pomace and seeds were enriched in fatty acids/esters, though not readily distinguishable from each other.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"2280-2300"},"PeriodicalIF":2.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141071755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of lipid metabolism after treatment of colon cancer mice with American ginseng vesicles.","authors":"Ling Weng, Min Zhao, Yumeng Zhang, Ruixiang Xu, Jiayi Zhang, Yingjie Wang, Yanxue Xu, Chunjie Zhao, Miao Wang","doi":"10.1002/pca.3367","DOIUrl":"10.1002/pca.3367","url":null,"abstract":"<p><strong>Introduction: </strong>Lipid molecules are present in tumours and play an important role in the anti-inflammatory response as well as in antiviral protection. Changes in the type and location of lipids in the intestine following exposure to environmental stressors play an important role in several disorders, including ulcerative colitis (UC), inflammatory bowel disease (IBD), and colorectal cancer.</p><p><strong>Objectives: </strong>The aim of this work is to provide a new theoretical basis for tumour initiation and development by accurately measuring the spatial distribution of lipids and metabolites in intestinal tissue. Spatial metabolomics allows the detection of samples with minimal sample volume by label-free imaging of complex samples in their original state. The distribution of lipid molecules in tumours has not been reported, although the distribution of lipid molecules in intestinal tissue has been reported in the literature.</p><p><strong>Methods: </strong>The range of lipid profiles in colon cancer mouse tumour tissue was compiled using a spatial metabolomics: lipid extraction method. The changes in lipid distribution in two regions after oral administration of American Ginseng (Panax quinquefolius L.) vesicles were also compared. Tumour tissue samples were extracted with 80% methanol-20% formic acid in water.</p><p><strong>Results: </strong>The resulting spatial metabolic profile allowed the identification of seven lipid classes in mouse tumours. The distribution of fibre tissue cells was 23.2% higher than tumour tissue cells, with the exception of the fatty acid (FA) species.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"2223-2227"},"PeriodicalIF":2.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141071753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced Methodologies for the Quality Control of Herbal Supplements and Regulatory Considerations.","authors":"Michael P Lindenmaier, Matthew W Bernart, Josef A Brinckmann","doi":"10.1002/pca.70000","DOIUrl":"10.1002/pca.70000","url":null,"abstract":"<p><strong>Introduction: </strong>Herbal supplements and OTC herbal drugs enjoy wide popularity with consumers but their quality has been questioned by genomic methods of testing. Due to complex regulatory environments in Europe and North America, the quality assurance of herbal preparations depends on protocols, which can significantly differ between the respective national and supranational drug control agencies. Modern methods of analysis combine genetic testing (DNA barcoding) with advanced chromatographic techniques as well as traditional microscopic and macroscopic tests to detect adulterants and undesirable constituents of herbs, including alkylphenols, aristolochic acids, and pyrrolizidine alkaloids.</p><p><strong>Objective: </strong>This review will give an account of current trends in herbal drug analysis and explain the shortcomings of existing methodologies. The article will also discuss regulatory protocols, compendial methods and differentiate between dietary supplement testing regimens and the requirements for approved herbal drugs. The purpose of this review is to document current trends in genetic testing and reveal future developments in drug analysis to reduce the possibility of adulterations and assure the authenticity of herbal products.</p><p><strong>Results: </strong>Chemometric methods and orthogonal approaches aid in the deconvolution of chromatographic and spectral data while expanding databases for nucleotide sequences and mineable spectra support method development in herbal analysis.</p><p><strong>Conclusion: </strong>Genetic testing of herbal products has further increased the capabilities to detect minute adulterations, but such assays are only meaningful in combination with chromatographic and spectroscopic analysis. Despite the advancement of genomic testing, chemometrics, UHPLC and mass spectrometry, cost-effective quality control techniques such as HPTLC in conjunction with microscopic and macroscopic examination remain important particularly in regulated environments.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"2417-2433"},"PeriodicalIF":2.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Pectic Polysaccharide From Stems and Leaves of Panacis Quinquefolii Radix: Preparation, Structural Characteristic, and Inhibition of Cardiac Hypertrophy.","authors":"Minghui Zhang, Yu Xu, Lei Zhang, Peipei Wang","doi":"10.1002/pca.3535","DOIUrl":"10.1002/pca.3535","url":null,"abstract":"<p><strong>Introduction: </strong>Panacis Quinquefolii Radix, a valued herb in traditional Chinese medicine, is frequently prescribed for its immunomodulatory effects and its potential to improve cardiovascular function. Recently, there is an increasing amount of research on ginseng polysaccharides, due to their variety of pharmacological activities, including anti-inflammatory, hepatoprotective, cardiovascular protective, and the improvement of intestinal function.</p><p><strong>Objective: </strong>This study investigates the efficacy of Panacis Quinquefolii Radix polysaccharides in alleviating cardiovascular diseases and to achieve the high-value utilization of ginseng by-products.</p><p><strong>Methodology: </strong>A pectic polysaccharide named XYSJY2-2 was extracted with hot water from stems and leaves of Panacis Quinquefolii Radix and further isolated by a DEAE Sepharose Fast Flow column and a Sephacry S-100 column. The structural characteristics of XYSJY2-2 was determined by monosaccharide composition, IR, NMR, and methylation analysis. The cardioprotective activity of this polysaccharide was evaluated in vitro and in vivo. With a relative molecular weight of 5.1 kDa, XYSJY2-2 is a type I rhamnogalacturonan featuring a core backbone made up of alternately linked rhamnose and galacturonic acid.</p><p><strong>Results: </strong>XYSJY2-2 effectively alleviated myocardial hypertrophy and remodeling induced by Ang II and TAC in vivo and in vitro, partly due to suppressing Nppa and Nppb expression.</p><p><strong>Conclusion: </strong>A pectic polysaccharide from stems and leaves of Panacis Quinquefolii Radix has the potential to alleviate cardiovascular disease.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"2434-2444"},"PeriodicalIF":2.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the quality difference between raw and ginger juice processed Magnoliae officinalis cortex by UPLC-Q-TOF-MS/MS and GC-MS coupled with color measurement.","authors":"Yufang Qi, Kewei Zhang, Yingtong Ren, Xingchen Fan, Jing Wang, Tulin Lu, Chunqin Mao","doi":"10.1002/pca.3424","DOIUrl":"10.1002/pca.3424","url":null,"abstract":"<p><strong>Introduction: </strong>Magnoliae officinalis cortex (MOC) has been used for thousands of years as a traditional Chinese herb. In Chinese Pharmacopoeia (2020 edition), it has two types of decoction pieces, raw Magnoliae officinalis cortex (RMOC) and ginger juice processed Magnoliae officinalis cortex (GMOC). The quality difference between RMOC and GMOC has not been explored systemically.</p><p><strong>Objective: </strong>This study aimed to discover the quality difference between RMOC and GMOC, and clarify the effect of ginger juice during processing comprehensively.</p><p><strong>Methods: </strong>Ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS) and gas chromatography-mass spectrometry (GC-MS) were applied to study the non-volatile and volatile components of RMOC and GMOC; electronic eye was applied for color measurement. Meanwhile, water processed Magnoliae officinalis cortex (WMOC) was studied as the blank sample.</p><p><strong>Results: </strong>There were 155 non-volatile and 72 volatile substances identified. Between RMOC and GMOC, 29 distinctive non-volatile and 34 distinctive volatile compounds were detected, among which 23 new compounds appeared and five compounds disappeared due to the addition of ginger juice during processing. The intensities of 12 common non-volatile compounds and the relative percentage contents of four common volatile compounds showed significant differences between RMOC and GMOC. In color measurement of RMOC, GMOC, and WMOC, 14 common compounds with significant differences were discovered related to their color values, and their mathematical prediction functions were built.</p><p><strong>Conclusion: </strong>There were significant differences between RMOC and GMOC; the processing mechanism of GMOC would be carried out based on the differential compounds in further investigation.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"2321-2335"},"PeriodicalIF":2.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}