Phytochemical Analysis最新文献

{"title":"Characterization of Quality Marker for Mume Fructus Pulp by Ultra-Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry-Based Untargeted Metabolomics Combined With Biological Activities.","authors":"Qiao Liu, Danni Chen, Huixin Liu, Miaomiao Wang, Yaqian Zhou, Hui Dai, Jian Liu, Wu Yin, Fangzhou Yin","doi":"10.1002/pca.3502","DOIUrl":"10.1002/pca.3502","url":null,"abstract":"<p><strong>Introduction: </strong>As a widely used Chinese herbal medicine, Mume Fructus pulp (MFP) has rich nutritional value and biological activity, but its quality control research is relatively scarce.</p><p><strong>Objectives: </strong>The objective of the study was to evaluate the quality difference between MFPs from different origins and its adulterant apricot pulp (APP), and to identify potential quality markers.</p><p><strong>Methods: </strong>The chemical compositions were identified by untargeted metabolomics analysis based on ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry combined with feature-based molecular networking. The effect of the test samples on the gene expression of the pro-inflammatory cytokines was investigated by RT-qPCR and ELISA analyses to determine their anti-inflammatory potential. Antioxidant activity was assessed by a free radical scavenging assay. Subsequently, the relationship between biological activity and chemical composition was analyzed by chemometrics, and the quality index of MFP was preliminarily determined. Based on network pharmacology and molecular docking, quality markers of MFP and their possible molecular mechanisms were further determined.</p><p><strong>Results: </strong>There were significant differences in the contents of flavonoids and organic acids, as well as the anti-inflammatory and antioxidant activities of MFPs from different habitats. Its adulterant APP was not only different from MFP in composition, but also had significantly weaker biological activity. Sixteen compounds were positively correlated with the antioxidant or anti-inflammatory effects of MFP. Combined with network pharmacology and molecular docking, citric acid, maleic acid, rutin, quercetin, isoquercetin, spiraeoside, and hesperidin were identified as potential quality markers of MFP.</p><p><strong>Conclusion: </strong>This study showed the potential of untargeted metabolomic analysis combined with bioactivity assays in the identification of herbal quality markers.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"1185-1201"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of a Unique Monoterpene Diperoxy Dimer From Ziziphora clinopodioides subsp. bungeana Together With Triterpenes With Antidiabetic Properties.","authors":"Milan Malaník, Jakub Treml, Renata Kubínová, Gabriela Vávrová, Michal Oravec, Jaromír Marek, Karlygash Zhaparkulova, Liliya Ibragimova, Tolkyn Bekezhanova, Aigerim Karaubayeva, Zuriyadda Sakipova, Karel Šmejkal","doi":"10.1002/pca.3505","DOIUrl":"10.1002/pca.3505","url":null,"abstract":"<p><strong>Introduction: </strong>Ziziphora clinopodioides subsp. bungeana (Juz.) Rech.f. is used in traditional medicine for various purposes. Previous phytochemical studies focused on phenolic compounds, but triterpenoids were almost overlooked.</p><p><strong>Objective: </strong>The study focused on the isolation of compounds with dual antidiabetic activity from the aerial parts of Z. clinopodioides subsp. bungeana.</p><p><strong>Materials and methods: </strong>Separation of CHCl₃-soluble fraction by silica gel column chromatography using different mobile phases and purification of compounds by semi-preparative HPLC or preparative TLC. The structures of pure compounds were elucidated by 1D and 2D NMR experiments along with HRMS. Compound 1 was additionally identified by the single crystal X-ray diffraction method. α-Glucosidase inhibitory assay and GLUT4 expression and translocation in C2C12 myotubes were conducted to evaluate antidiabetic potential of isolated compounds.</p><p><strong>Results: </strong>This phytochemical study led to the isolation of 20 compounds, including a unique monoterpene diperoxy dimer (1). Compounds 7 and 9-11 displayed more potent α-glucosidase inhibitory activity (IC₅₀ 45.3-135.3 μM) than acarbose used as a positive control (IC₅₀ 264.7 μM), while only pomolic acid (5) increased GLUT4 translocation in C2C12 myotubes in a significant manner.</p><p><strong>Conclusion: </strong>Extensive chromatographic separation led to the isolation and identification of a unique monoterpene diperoxy dimer (1) from aerial parts of Z. clinopodioides subsp. bungeana. Some triterpenes inhibited α-glucosidase, another increased GLUT4 translocation. Although none of the isolated compounds demonstrated dual antidiabetic activity, selected triterpenes proved to be potent antidiabetic agents in vitro.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"1223-1230"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12129716/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Advances on the Chemical Composition, Pharmacological Properties, and Product Development of Morus alba.","authors":"Zixuan Yan, Shamukaer Alimujiang, Yao Zhang, Jianqiang Zhao, Yunfei Hu, Wenlong Li","doi":"10.1002/pca.3544","DOIUrl":"https://doi.org/10.1002/pca.3544","url":null,"abstract":"<p><strong>Introduction: </strong>Morus alba, a member of the Moraceae family, has a long history of medicinal use in China. Its branches, fruits, leaves, and root bark are rich in various bioactive compounds, including flavonoids, alkaloids, and other phytochemicals. These plant parts exhibit a broad spectrum of pharmacological activities, such as antidiabetic, antitumor, antibacterial, and antioxidant effects. Owing to these properties, various parts of M. alba have been developed into functional foods and health products, attracting considerable public and scientific interest.</p><p><strong>Objectives: </strong>This review aims to summarize current knowledge on the phytochemistry, pharmacological activities, and product development related to different parts of M. alba, with particular emphasis on their respective roles in the development of functional products.</p><p><strong>Methods: </strong>Relevant literature was retrieved from major scientific databases, including PubMed, Web of Science, ScienceDirect, and Google Scholar. Information related to the phytochemistry, pharmacological effects, and product development of various parts of M. alba was systematically collected and analyzed.</p><p><strong>Results: </strong>This review summarizes the chemical composition of different parts of M. alba and their corresponding pharmacological properties. It also highlights recent advancements in the development of functional products derived from M. alba.</p><p><strong>Conclusions: </strong>M.orus alba contains a wide array of bioactive compounds with promising pharmacological potential. Its various parts have demonstrated potential in the prevention and management of chronic diseases and have been increasingly utilized in the development of functional health products. Further interdisciplinary studies are warranted to facilitate its translational application in modern healthcare.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144192066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolite Screening From Pinus pinea Needles Reveals (+)-Isocupressic Acid as a Key Phytotoxin for Weed Management.","authors":"Hajer Hlaili, Jesús G Zorrilla, Maria Michela Salvatore, Mejda Abassi, Maria Teresa Russo, Miriam I Martínez-González, Marina DellaGreca, Alessio Cimmino, Francisco A Macías, Anna Andolfi, Rosa M Varela, Marco Masi","doi":"10.1002/pca.3546","DOIUrl":"https://doi.org/10.1002/pca.3546","url":null,"abstract":"<p><strong>Introduction: </strong>Weeds are a major threat to crop productivity, competing for essential resources and often developing resistance to herbicides, which underscores the need for novel, sustainable control strategies. The valorization of agricultural and forestry underutilized byproducts, such as plant needles, presents a promising opportunity for developing eco-friendly bioherbicides based on allelopathy.</p><p><strong>Objectives: </strong>This study investigates the phytotoxicity of Pinus pinea needle extracts and metabolites to evaluate their potential for controlling dicotyledonous weeds.</p><p><strong>Material and methods: </strong>The chemical characterization of extracts and isolated compounds was performed via GC-MS, NMR, and optical methods while phytotoxicity bioassays were carried out using the herbicides Pacifica Plus (Bayer CropScience) and pendimethalin, the active ingredient in Stone Aqua (Tokyo Chemical Industry), as positive controls.</p><p><strong>Results: </strong>The dichloromethane extract exhibited the highest phytotoxicity, significantly inhibiting Portulaca oleracea and Plantago lanceolata weeds. GC-MS analysis revealed an array of aromatic compounds of interest for phytochemical research, and through bio-guided purification, five lignans and the diterpenic acid (+)-isocupressic acid were isolated. (+)-Isocupressic acid showed the strongest phytotoxicity on P. oleracea, particularly on root growth (-83% ± 4% at 1000 μM), which could be correlated with structural moieties in its structure (fused-ring scaffold with an exocyclic double bond, an exocyclic chain containing a double bond or hydroxyl group, and a carboxylic acid group), a number of H-bond donors ≤ 2, and higher lipophilicity (Clog p = 5.11). Some lignans displayed mild inhibitory or stimulatory effects on P. lanceolata.</p><p><strong>Conclusion: </strong>P. pinea needle extracts and metabolites have demonstrated potential as natural bioherbicides for weed management. Further research is prompted to explore large-scale applicability, environmental safety through ecotoxicological studies, and optimized formulations to enhance their practical use in sustainable agriculture.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144192153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality Analysis of Curcumae Radix From Different Sources Based on the Three-Dimensional Evaluation System of \"Morphology-Composition-Efficacy\".","authors":"Yv Wang, Zhewen Zhang, Liu Yang, Pei Ye, Yv Ye, Xiaoning Li, Weifeng Du","doi":"10.1002/pca.3541","DOIUrl":"https://doi.org/10.1002/pca.3541","url":null,"abstract":"<p><strong>Introduction: </strong>A major limitation in current Traditional Chinese Medicine (TCM) research lies in its overreliance on single-factor analyses of chemical composition or pharmacodynamics, or simplistic combined models. While these approaches partially elucidate TCM characteristics, they fail to comprehensively capture its holistic complexity and inherent quality.</p><p><strong>Objectives: </strong>The three-dimensional comprehensive evaluation method of \"morphology-composition-efficacy (MCE)\" was creatively introduced in this study, and the quality of four different varieties of Curcumae Radix was deeply and meticulously studied.</p><p><strong>Materials and methods: </strong>Curcumae Radix from different sources was collected, and its external morphology (volume and weight, color and smell), internal composition (extract, fingerprint and literature knowledge network diagram) and drug effect (efficacy of zebrafish on improving qi stagnation and blood stasis) were analyzed. The differences in composition and morphology of four kinds of Curcumae Radix were studied by HPLC fingerprint, chromaticity identification model, and BP-ANN classification model. Secondly, through the experiment of zebrafish \"qi stagnation and blood stasis\" model, the improvement effect of four kinds of turmeric on \"qi stagnation and blood stasis\" was explored. Finally, the relationship between the three dimensions of MCE of Curcumae Radix was discussed through Pearson analysis.</p><p><strong>Results: </strong>The pharmacodynamic experiment of zebrafish confirmed that all four kinds of Curcumae Radix showed the activity of treating \"qi stagnation and blood stasis\" and Curcuma phaeocaulis Val. (LSYJ) had the best effect comprehensively. And through Pearson analysis, it can be concluded that the smaller the external volume and weight of Curcumae Radix, the stronger the characteristic odor (W5S, W1W, and W2W), the closer the powder color is to red and yellow, the higher the internal extract (JCW) content of Curcumae Radix, the higher the contents of germacrone (JMT), furanodiene (FNEX), and β-elemene (β-LXX), and the better the efficacy of improving the disease of \"qi stagnation and blood stasis,\" among which JMT is the key drug component.</p><p><strong>Conclusion: </strong>The research demonstrates the value of a three-dimensional evaluation system for TCM quality assessment, providing insights into the relationship between morphology, composition, and efficacy. It offers a new perspective for quality control and evaluation of Curcumae Radix and provides a scientific basis for the clinical treatment of \"qi stagnation and blood stasis\" related diseases.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144192065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ATR-FTIR Coupled With Machine Learning Provides a Fast Method for Identifying and Distinguishing 55 Varieties of Fruit-Derived Medicinal Materials.","authors":"Wen-Jie Zhao, Ya-Ling An, Chun-Qian Song, Yu-Shi Huang, Li-Jie Zhang, Kang-Nan Liu, Zhen-Wei Li, Xiao-Kang Liu, Dai-di Zhang, De-An Guo","doi":"10.1002/pca.3545","DOIUrl":"https://doi.org/10.1002/pca.3545","url":null,"abstract":"<p><strong>Introduction: </strong>Fruit-derived medicinal materials (FDMM) are extensively utilized in daily life, yet the market is beset by substantial variety confusion, which undermines consumer rights and well-being. Consequently, accurate identification of these materials is essential for guaranteeing their quality, effectiveness, and safety.</p><p><strong>Objectives: </strong>This study aimed to combine attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and machine learning (ML) techniques to differentiate and identify 55 kinds of FDMM.</p><p><strong>Materials and methods: </strong>A total of 861 sample batches were collected, with 721 allocated for model establishment and 140 for independent validation. A PLS-DA model alongside nine machine learning algorithms-namely support vector machine (SVM), tree, K-nearest neighbor (KNN), discriminant, ensemble, support vector machine kernel (SVMK), logistic regression kernel (LRK), naive Bayes (NB), and neural network (NN)-were constructed. Considering both accuracy and computational efficiency, the optimal model was selected and evaluated in terms of its accuracy, precision, recall, and F1-score. The optimal model was further validated using 140 newly collected samples to ensure its long-term stability after several months.</p><p><strong>Results: </strong>Among the 10 classification models, the KNN model showed exceptional classification capability, with all evaluation metric exceeding 0.98. The KNN model was validated by the new 140 samples with a prediction accuracy of 85.7%, confirming its capability in distinguishing most FDMM.</p><p><strong>Conclusion: </strong>The application of ATR-FTIR technology combined with the robust classification capabilities of ML models enabled rapid and accurate differentiation and identification of 55 FDMM, which contributed to ensuring their quality.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144174526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted Neuroprotection in Sporadic Alzheimer's Disease: UPLC-ESI-MS/MS Profiling and Bilosome-Mediated Delivery of Crateva magna and Its Endophytic Fungal Extracts.","authors":"Aya N Talaat, Mohamed S Elnaggar, Nehal Ibrahim, Iriny M Ayoub, Haidy Abbas, Nesrine El Sayed, Rola M Labib, Abdel Nasser B Singab","doi":"10.1002/pca.3540","DOIUrl":"https://doi.org/10.1002/pca.3540","url":null,"abstract":"<p><strong>Introduction: </strong>Crateva magna (Cm) was utilized as a folkloric medicine against neurological disorders.</p><p><strong>Objectives: </strong>This study aimed to investigate the phytochemical profile of Cm leaf extract and its endophytic fungus, Nigrospora oryzae (No) extract. Additionally, the neuroprotective potential of their optimized bilosomes (BLs) will be assessed as an approach to Alzheimer's disease (AD) treatment.</p><p><strong>Materials and methods: </strong>UPLC-ESI-MS/MS chemical profiling was performed. In vitro anti-Alzheimer activity of Cm and No extracts was evaluated against AChE and BACE1 enzymes. Cm-BLs and No-BLs were prepared using the thin-film hydration technique. In vivo anti-Alzheimer potential was assessed in a streptozotocin (STZ)-induced sporadic AD mouse model. Behavioral assays, neurochemical assays, RT-PCR analysis, histopathological examination, and immunohistochemical analysis were performed.</p><p><strong>Results: </strong>Chemical profiling revealed diverse metabolites from various chemical classes. The major class identified in Cm extract was flavonoids, e.g., kaempferol-O-hexoside, whereas in No extract, it was alkaloids, e.g., phenazine carboxamide. The neuropathological markers (Aβ1-42, IL-6, and p-Tau protein) were reduced by ≈50% and 60% in mice receiving Cm-BLs and No-BLs, respectively, relative to the STZ group. Also, the BLs exhibited the greatest ability to downregulate the expression of p-JNK, p-P38, and p-ERK in the brain. Histopathological examination revealed that No-BLs showed the highest protection for the hippocampus and cerebral cortex regions. Also, it revealed a significantly decreased reaction for NFκB in cerebral cortex neurons.</p><p><strong>Conclusion: </strong>Cm-BLs and No-BLs exhibit considerable potential as novel adjuvant therapies for AD, utilizing natural bioactive compounds to improve the efficiency of targeted drug delivery and enhance therapeutic outcomes.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous Quantification of 22 Compounds in Talinum paniculatum Root and Proposal of Three Anti-Counterfeiting Quality Markers Using a Library-Aided UHPLC-Q-Orbitrap-MS/MS Method.","authors":"Jianwu Li, Hanxiao Chai, Xican Li, Shaoman Chen, Rongxin Cai, Lanying Wang, Xiaoqiong Xu, Zhenhui Chen","doi":"10.1002/pca.3543","DOIUrl":"https://doi.org/10.1002/pca.3543","url":null,"abstract":"<p><strong>Background: </strong>Talinum paniculatum root (pseudo ginseng in Chinese) is a widely distributed medicinal plant material in Asia, Africa, and America. However, its chemistry remains poorly understood nowadays.</p><p><strong>Methods: </strong>Fresh Talinum paniculatum root was treated for the preparation of a lyophilized aqueous extract. The extract thereafter was subjected to qualitative and quantitative analysis using the library-aided ultra-high-performance liquid chromatography-quadrupole-Exactive-Orbitrap-tandem mass spectrometry (UHPLC-Q-Exactive-Orbitrap-MS/MS) technology.</p><p><strong>Results: </strong>Based on MS/MS elucidation and comparison with the authentic standards in the library of UHPLC-Q-Exactive-Orbitrap-MS/MS apparatus, 22 compounds were accurately identified. Information retrieval suggested that 18 compounds belonged to \"unexcavated\" ones, which were found in the plant for the first time. Subsequently, all 22 compounds (including 18 \"unexcavated\" ones) were further quantified using the corresponding linear regression equations of authentic standards. All 22 compounds exhibited different contents from each other in the quantitative analysis. Particularly, synephrine had the highest content (8.159 ± 1.443 mg/g extract), and α-mangostin showed the lowest content (0.001 ± 0.000 mg/g extract), while chikusetsusaponin Iva displayed a moderate content (0.737 ± 0.274 mg/g extract).</p><p><strong>Conclusion: </strong>On the basis of these results and relevant principles, synephrine, N-trans-feruloyltyramine, and chikusetsusaponin Iva are recommended as the anti-counterfeiting Q-markers of T. paniculatum root. These findings will help to understand the substance basis of the traditional medicinal functions of T. paniculatum root and to find a suitable quality-control method.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Inflammatory Saponins From an Edible Aquatic Plant Nymphoides hydrophylla.","authors":"Ching-Yeu Chen, Tzu-Peng Cheng, Tsong-Long Hwang, Yong-Siang Hou, Limbadri Salendra, Tzi-Yi Ke, Yuan-Bin Cheng","doi":"10.1002/pca.3539","DOIUrl":"https://doi.org/10.1002/pca.3539","url":null,"abstract":"<p><strong>Introduction: </strong>The present investigation of Nymphoides hydrophylla is a Taiwan indigenous edible aquatic plant, belongs to the Menyanthaceae family, and has been used as a traditional food with potential medicinal values against fever, insect bites, ulcer, and skin diseases. Interestingly, we found that the plant extract showed a significant anti-inflammatory effect.</p><p><strong>Objectives: </strong>The aim is isolation, structure elucidation, and anti-inflammatory evaluation of secondary metabolites from N. hydrophylla.</p><p><strong>Material and methods: </strong>This study involves the bioactive isolation and structural studies of the pure compounds determined by NMR, UV, IR spectroscopies, and HRMS. The anti-inflammatory activity of the pure compounds was evaluated in vitro. In addition, isolates showed remarkable anti-inflammatory activities to regulate superoxide anion generation and elastase release in fMLF/CB-induced human neutrophils.</p><p><strong>Results: </strong>This study conducted 10 oleanane-type saponins with oligoglycoside linkage, including two new (1 and 2) identified from the butanol extract of N. hydrophylla. As a result, Compounds 1 and 2, representing new triterpene saponins, have not been previously reported. In vitro anti-inflammatory tests revealed that Compounds 1, 5-7, 9, and 10 showed significant anti-inflammatory effects by inhibiting superoxide anion generation with IC₅₀ values of 1.62, 0.92, 0.28, 2.69, 0.29, and 0.15 μM, respectively, whereas compounds 1, 5, 6, 9, and 10 showed potential inhibition on elastase release in human neutrophils, with IC₅₀ values of 0.19, 1.12, 0.70, 0.68, and 0.51 μM, respectively.</p><p><strong>Conclusion: </strong>Our findings suggested that the components of N. hydrophylla have the potential to develop anti-inflammatory agents.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhododendri Daurici Folium: An Overview of Studies on the Study of Herbal Medicine, Chemical Composition, Pharmacological Action, Clinical Application, and Quality Control.","authors":"Chenliang Li, Huixuan Tian, Wei Wu, Lingyang Kong, Jiao Xu, Lihong Zhang","doi":"10.1002/pca.3537","DOIUrl":"https://doi.org/10.1002/pca.3537","url":null,"abstract":"<p><p>The Rhododendri Daurici Folium (RDF) represents the desiccated foliage of Rhododendron dauricum L., belonging to the Rhododendron family. RDF abounds in diverse chemical elements, predominantly flavonoids, volatile oils, phenols, coumarin, and lignans. RDF functions as a cough and expectorant agent, primarily employed in the management of acute and chronic bronchitis and pharyngitis. RDF is also known for its pharmacological properties, including antibacterial, cardiotonic, antihypertensive, and anticancer properties. RDF is frequently utilized as a potent ingredient in Yingshanhong tablets, compound RDF syrup, XiaoKeChuan syrup, and various traditional Chinese medicinal concoctions, and it holds significant importance in clinical settings. This article's data were sourced from publications including PubMed, CNCK, SCI-HUB, Web of Science, Scopus, Chinese Pharmacopoeia, Chinese Dictionary of Traditional Chinese Medicine, Chinese Flora, and associated studies. The present document provides a comprehensive examination of the herbal properties, pharmacological effects, chemical makeup, clinical application, quality assurance, and safety assessments of RDF, serving as a guide for advancing and applying traditional Chinese medicine RDF.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}