Determination of the Major Bioactive Component of Silybum marianum in Nutricosmetics by a HPLC Method With Amperometric Detection and UAE Pretreatment.

IF 3 3区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS
Lucía Abad-Gil, M Jesús Gismera, M Teresa Sevilla, Jesús R Procopio
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引用次数: 0

Abstract

Introduction: Nutricosmetics derived from Silybum marianum, known for their anti-inflammatory and hepatoprotective properties, necessitate accurate quantification of silybin, a key bioactive component.

Objectives: This study aims to develop a novel high-performance liquid chromatography (HPLC) method with amperometric detection (HPLC-ECD) for the precise determination of silybin. An ultrasound-assisted extraction (UAE) procedure is also established for solid sample preparation prior to chromatographic analysis.

Materials and methods: Chromatographic separation of silybin was performed on a C18 column and using methanol-0.035 M potassium phosphate (pH 4.0) at 1.0 mL min-1 flow rate as mobile phase in gradient mode. The electrochemical detection (ECD) of silybin was carried out on a glassy carbon electrode (GCE) at +1.10 V versus Ag/AgCl. The UAE procedure for silybin extraction from solid samples was performed by 15 min sonication in an ultrasonic bath (80 kHz and 100% power) at room temperature.

Results: Under the optimal chromatographic conditions, silybin diastereoisomers (silybin A and silybin B) can be separated from other S. marianum flavonolignans in less than 20 min, with a detection limit (LOD) of 0.060 mg L-1 and a reproducibility (RSD) of 5%. This method was successfully applied to analyze silymarin-containing products with recoveries close to 100%.

Conclusions: This work presents the first HPLC method for silybin determination using an amperometric detector with a GCE. The LOD is competitive in comparison with previously published HPLC-DAD methods. This HPLC-ECD method allows silybin diastereoisomers identification without interferences of other flavonolignans present in silymarin extracts.

采用安培检测和 UAE 预处理的高效液相色谱法测定营养化妆品中的水飞蓟主要生物活性成分
简介:水飞蓟具有抗炎和保护肝脏的特性,从水飞蓟中提取的营养化妆品需要对其关键的生物活性成分水飞蓟宾进行精确定量:本研究旨在开发一种新型高效液相色谱-安培检测法(HPLC-ECD),用于水飞蓟宾的精确测定。同时还建立了超声辅助萃取(UAE)程序,用于色谱分析前的固体样品制备:水飞蓟宾的色谱分离采用 C18 色谱柱,流动相为甲醇-0.035 M 磷酸二氢钾(pH 4.0),流速为 1.0 mL min-1,梯度模式。水飞蓟宾的电化学检测(ECD)在玻璃碳电极(GCE)上进行,相对于 Ag/AgCl 的电压为 +1.10 V。从固体样品中提取水飞蓟宾的 UAE 程序是在室温下在超声波浴(80 kHz,100% 功率)中超声 15 分钟:在最佳色谱条件下,水飞蓟宾非对映异构体(水飞蓟宾 A 和水飞蓟宾 B)可在 20 分钟内从其他水飞蓟黄酮类化合物中分离出来,检出限(LOD)为 0.060 mg L-1,重现性(RSD)为 5%。该方法成功用于分析含水飞蓟素的产品,回收率接近100%:本研究首次采用高效液相色谱法测定水飞蓟宾。与之前公布的 HPLC-DAD 方法相比,该方法的 LOD 具有竞争力。该 HPLC-ECD 方法可鉴定水飞蓟宾非对映异构体,且不会受到水飞蓟素提取物中其他黄酮类木脂素的干扰。
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来源期刊
Phytochemical Analysis
Phytochemical Analysis 生物-分析化学
CiteScore
6.00
自引率
6.10%
发文量
88
审稿时长
1.7 months
期刊介绍: Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.
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