Rapid Evaluation of Qingpi Products Using E-Eye, Fast GC e-Nose, and FT-NIR.

IF 3 3区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS
Xiaoyu Fan, Jiayi Wang, Binghan Liu, Mingxuan Li, Peijun Sun, Jialei Wu, Shuai Zhang, Fangzhou Yin, Jining Liu, Tulin Lu, Lihong Chen
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Abstract

Introduction: Current standards for identifying the characteristics of Qingpi (QP) primarily depend on traditional empirical methods, which are both time-consuming and labor-intensive. Therefore, there is significant potential for developing a rapid and nondestructive identification method.

Objective: This work aims to realize fast and effective identification of QP and its processed products, to provide a basis for quality assurance and monitoring of QP and its processed products, and to provide reference for guiding the practical work of traditional Chinese medicine (TCM) identification.

Methods: In this study, we employed three advanced technologies-electronic eye (E-eye), fast gas chromatography electronic nose (Fast GC e-nose), and Fourier transform near-infrared (FT-NIR) spectroscopy-to analyze the color, odor, and absorbance of QP samples.

Results: The E-eye digitized the appearance color of various QP samples, and subsequent discriminant analysis, combined with statistical methods, confirmed the feasibility of the discriminant function obtained. Additionally, the Fast GC e-nose provided valuable odor information, identifying 18 distinct odor components. Based on variable importance in projection (VIP) analysis, four components were hypothesized as potential odor markers for distinguishing QP raw products (SQP) from vinegar processed products (CQP). According to the accuracy of the support vector machine (SVM) classification model, the NIR preprocessing method is screened. Alongside SVM, three classification models are chosen for simultaneous evaluation and verification. Notably, the test set recognition rate for all four classification models reached 100%.

Conclusion: E-eye, Fast GC e-nose, and NIR technology enable rapid, nondestructive identification and preliminary quality evaluation of QP products.

用电子眼、快速气相色谱电子鼻和傅里叶变换近红外光谱快速评价青皮产品。
导言:目前鉴别清皮特征的标准主要依靠传统的经验方法,既费时又费力。因此,开发一种快速、无损的鉴定方法具有很大的潜力。目的:实现QP及其制品的快速有效鉴定,为QP及其制品的质量保证和监控提供依据,为指导中药鉴定的实际工作提供参考。方法:采用电子眼(E-eye)、快速气相色谱电子鼻(fast GC e-nose)和傅里叶变换近红外(FT-NIR)光谱技术对QP样品的颜色、气味和吸光度进行分析。结果:E-eye对各种QP样品的外观颜色进行数字化处理,并结合统计学方法进行判别分析,证实了所得判别函数的可行性。此外,快速气相色谱电子鼻提供了有价值的气味信息,识别出18种不同的气味成分。基于投影变量重要度(VIP)分析,假设4种成分可作为区分食醋原料(SQP)和食醋加工产品(CQP)的潜在气味标记。根据支持向量机(SVM)分类模型的精度,筛选近红外预处理方法。在支持向量机的同时,选择了三种分类模型进行评价和验证。值得注意的是,四种分类模型的测试集识别率均达到100%。结论:E-eye技术、快速气相色谱电子鼻技术和近红外技术可实现QP产品的快速、无损鉴定和初步质量评价。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Phytochemical Analysis
Phytochemical Analysis 生物-分析化学
CiteScore
6.00
自引率
6.10%
发文量
88
审稿时长
1.7 months
期刊介绍: Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.
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