{"title":"用当归桂枝煎剂和散剂干预血瘀证大鼠尿液的代谢组学分析和肠道微生物群的16S rRNA测序","authors":"Jiwei Yu, Qianyi Wang, Yunyuan Nong, Linlin Feng, Shulin Huang, Zhiheng Su","doi":"10.1002/pca.3518","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Blood stasis syndrome (BSS) is one of the common syndromes in traditional Chinese medicine. The Chinese medicine compound composed of \"Danggui-Guizhi (DG-GZ)\"as the core has the effect of reducing blood stasis and promoting blood circulation. However, the difference of efficacy of DG-GZ traditional decoction and dispensing granules in reducing blood stasis are still unclear.</p><p><strong>Objective: </strong>Investigation of the difference of decoction and dispensing granules of Danggui (DG) and Guizhi (GZ) in blood stasis syndrome (BSS) in an animal study (Six groups, n = 6).</p><p><strong>Methodology: </strong>All rats were randomly divided into six groups: control, model, Danggui-Guizhi dispensed granules ( DGK-GZK), Danggui-Guizhi decoction (DG-GZ), Danggui granules-Guizhi decoction (DGK-GZ), and Danggui decoction -Guizhi granules (DG-GZK) groups (n = 6). The blood stasis model was constructed by exposing rats to ice-cold water. Whole blood viscosity (WBV) was used to compare the effects of DG-GZ (4:3 w/w) decoction and dispensing granules in improving BSS. Metabolomics analysis of urine and 16S rRNA gene sequencing were used to investigate metabolic analysis and gut microbiota changes in rats with BSS.</p><p><strong>Results: </strong>The results of WBV showed that DG-GZ and its granules exhibited efficacy against blood stasis, and the effect of DGK-GZK in relieving blood stasis was greater than that of other administration groups. Urine metabolomics demonstrated that DGK-GZK could reverse the abnormal levels of butyrate, citrate, taurine, creatinine, glucose, and other metabolites in BSS rats. DGK-GZK could promote the enrichment of the g__Prevotellaceae_NK3B31_group, g__Ligilactobacillus, and g__Roseburia.</p><p><strong>Conclusions: </strong>The effect of DGK-GZK intervention in BSS was stronger than that of other compound groups. This study also provides basic experimental data for exploring the difference in efficacy between traditional decoction and dispensing granules.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":3.0000,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Metabolomics Analysis of Urine and 16S rRNA Sequencing of Gut Microbiota in Rats With Blood Stasis Syndrome After Intervention With Danggui-Guizhi Decoction and Dispensing Granules.\",\"authors\":\"Jiwei Yu, Qianyi Wang, Yunyuan Nong, Linlin Feng, Shulin Huang, Zhiheng Su\",\"doi\":\"10.1002/pca.3518\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Blood stasis syndrome (BSS) is one of the common syndromes in traditional Chinese medicine. The Chinese medicine compound composed of \\\"Danggui-Guizhi (DG-GZ)\\\"as the core has the effect of reducing blood stasis and promoting blood circulation. However, the difference of efficacy of DG-GZ traditional decoction and dispensing granules in reducing blood stasis are still unclear.</p><p><strong>Objective: </strong>Investigation of the difference of decoction and dispensing granules of Danggui (DG) and Guizhi (GZ) in blood stasis syndrome (BSS) in an animal study (Six groups, n = 6).</p><p><strong>Methodology: </strong>All rats were randomly divided into six groups: control, model, Danggui-Guizhi dispensed granules ( DGK-GZK), Danggui-Guizhi decoction (DG-GZ), Danggui granules-Guizhi decoction (DGK-GZ), and Danggui decoction -Guizhi granules (DG-GZK) groups (n = 6). The blood stasis model was constructed by exposing rats to ice-cold water. Whole blood viscosity (WBV) was used to compare the effects of DG-GZ (4:3 w/w) decoction and dispensing granules in improving BSS. Metabolomics analysis of urine and 16S rRNA gene sequencing were used to investigate metabolic analysis and gut microbiota changes in rats with BSS.</p><p><strong>Results: </strong>The results of WBV showed that DG-GZ and its granules exhibited efficacy against blood stasis, and the effect of DGK-GZK in relieving blood stasis was greater than that of other administration groups. Urine metabolomics demonstrated that DGK-GZK could reverse the abnormal levels of butyrate, citrate, taurine, creatinine, glucose, and other metabolites in BSS rats. DGK-GZK could promote the enrichment of the g__Prevotellaceae_NK3B31_group, g__Ligilactobacillus, and g__Roseburia.</p><p><strong>Conclusions: </strong>The effect of DGK-GZK intervention in BSS was stronger than that of other compound groups. This study also provides basic experimental data for exploring the difference in efficacy between traditional decoction and dispensing granules.</p>\",\"PeriodicalId\":20095,\"journal\":{\"name\":\"Phytochemical Analysis\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2025-02-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Phytochemical Analysis\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1002/pca.3518\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytochemical Analysis","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1002/pca.3518","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Metabolomics Analysis of Urine and 16S rRNA Sequencing of Gut Microbiota in Rats With Blood Stasis Syndrome After Intervention With Danggui-Guizhi Decoction and Dispensing Granules.
Introduction: Blood stasis syndrome (BSS) is one of the common syndromes in traditional Chinese medicine. The Chinese medicine compound composed of "Danggui-Guizhi (DG-GZ)"as the core has the effect of reducing blood stasis and promoting blood circulation. However, the difference of efficacy of DG-GZ traditional decoction and dispensing granules in reducing blood stasis are still unclear.
Objective: Investigation of the difference of decoction and dispensing granules of Danggui (DG) and Guizhi (GZ) in blood stasis syndrome (BSS) in an animal study (Six groups, n = 6).
Methodology: All rats were randomly divided into six groups: control, model, Danggui-Guizhi dispensed granules ( DGK-GZK), Danggui-Guizhi decoction (DG-GZ), Danggui granules-Guizhi decoction (DGK-GZ), and Danggui decoction -Guizhi granules (DG-GZK) groups (n = 6). The blood stasis model was constructed by exposing rats to ice-cold water. Whole blood viscosity (WBV) was used to compare the effects of DG-GZ (4:3 w/w) decoction and dispensing granules in improving BSS. Metabolomics analysis of urine and 16S rRNA gene sequencing were used to investigate metabolic analysis and gut microbiota changes in rats with BSS.
Results: The results of WBV showed that DG-GZ and its granules exhibited efficacy against blood stasis, and the effect of DGK-GZK in relieving blood stasis was greater than that of other administration groups. Urine metabolomics demonstrated that DGK-GZK could reverse the abnormal levels of butyrate, citrate, taurine, creatinine, glucose, and other metabolites in BSS rats. DGK-GZK could promote the enrichment of the g__Prevotellaceae_NK3B31_group, g__Ligilactobacillus, and g__Roseburia.
Conclusions: The effect of DGK-GZK intervention in BSS was stronger than that of other compound groups. This study also provides basic experimental data for exploring the difference in efficacy between traditional decoction and dispensing granules.
期刊介绍：
Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.
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