Comprehensive Quality Evaluation and Botanical Differentiation of Typhae Pollen Using UHPLC-DAD/Q-TOF-MS and Multivariate Chemometric Analysis.

IF 3 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Phytochemical Analysis Pub Date : 2025-02-20 DOI:10.1002/pca.3519

Fang Tian, Ying Zhang, Lixia Chen, Jieyi Wu, Hui Cao, Menghua Wu, Zhiguo Ma

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引用次数: 0

Abstract

Introduction: In China, Typhae Pollen (TP) has been extensively utilized as a medicinal product, which is the dried pollen from the male inflorescences of Typha angustifolia L. (TA), Typha orientalis Presl (TO), or species within the same genus. However, existing methods for differentiating the botanical origin and controlling the quality of TP are confusing, unreliable, and unsystematic.

Objectives: This study aims to elucidate the microstructural similarities and differences between pollen grains of the two species and to develop a comprehensive method for the qualitative and quantitative analysis of multiple flavonoids and phenolic acids in TP.

Methodology: Scanning electron microscopy (SEM) was employed to examine the dried pollen grains. A comprehensive analytical approach was developed using ultra-high performance liquid chromatography with diode array detection and quadrupole-time-of-flight mass spectrometry to quantitatively and qualitatively analyze 17 flavonoids and phenolic acids. Discrepancies in constituents were further explored using partial least squares discrimination analysis and hierarchical clustering analysis.

Results: Microscopic examination revealed distinct differences between the pollen grains of the two species, which could be distinguished using SEM. The analytical methods established for these 17 constituents-comprising 13 flavonoids and 4 phenolic acids-proved reliable and accurate. The flavonoid glycosides in TP could be categorized into three distinct groups. In TA, the average contents of constituents 1, 2, 3, 4, 8, and 12 were higher than in TO, whereas the reverse was true for constituents 7, 14, 16, and 17. Constituents 9, 10, and 13 were unique to TO. And constituent 14 should be chosen as a more appropriate quality indicator for TO. Chemometric techniques effectively differentiated between TA and TO.

Conclusions: This study contributes to enhancing quality control and facilitating botanical origin identification of TP and provides an experimental foundation for improving its standards.

查看原文本刊更多论文

利用UHPLC-DAD/Q-TOF-MS和多元化学计量分析综合评价和鉴别黄斑病花粉。

在中国，Typhae Pollen （TP）作为一种医药产品被广泛利用，它是Typha angustifolia L. （TA）、Typha orientalis Presl （TO）或同一属物种雄花序的干燥花粉。然而，现有的鉴别植物来源和质量控制方法混乱、不可靠、不系统。目的：本研究旨在阐明两种植物花粉粒的微观结构异同，并建立一种综合定性和定量分析TP中多种黄酮类和酚酸的方法。方法：采用扫描电镜（SEM）对干燥的花粉粒进行观察。采用超高效液相色谱二极管阵列检测和四极杆飞行时间质谱联用技术对17种黄酮类和酚类酸进行了定量和定性分析。利用偏最小二乘判别分析和层次聚类分析进一步探讨成分差异。结果：两种植物的花粉粒在显微镜下有明显的差异，可以通过扫描电镜进行区分。所建立的分析方法可靠、准确，其中黄酮类化合物13种，酚酸类4种。TP中的黄酮类苷可分为三个不同的类群。在TA中，组分1、2、3、4、8和12的平均含量高于TO，而组分7、14、16和17的平均含量则相反。成分9、10和13是to独有的。成分14应该作为TO的一个更合适的质量指标。化学计量技术有效地区分了TA和TO。结论：本研究有助于加强TP的质量控制和植物来源鉴定，为提高TP的标准提供实验依据。

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来源期刊

Phytochemical Analysis 生物-分析化学

CiteScore

6.00

自引率

6.10%

发文量

审稿时长

1.7 months

期刊介绍： Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.