Phytochemical Analysis最新文献

{"title":"Quality Analysis of Curcumae Radix From Different Sources Based on the Three-Dimensional Evaluation System of \"Morphology-Composition-Efficacy\".","authors":"Yv Wang, Zhewen Zhang, Liu Yang, Pei Ye, Yv Ye, Xiaoning Li, Weifeng Du","doi":"10.1002/pca.3541","DOIUrl":"https://doi.org/10.1002/pca.3541","url":null,"abstract":"<p><strong>Introduction: </strong>A major limitation in current Traditional Chinese Medicine (TCM) research lies in its overreliance on single-factor analyses of chemical composition or pharmacodynamics, or simplistic combined models. While these approaches partially elucidate TCM characteristics, they fail to comprehensively capture its holistic complexity and inherent quality.</p><p><strong>Objectives: </strong>The three-dimensional comprehensive evaluation method of \"morphology-composition-efficacy (MCE)\" was creatively introduced in this study, and the quality of four different varieties of Curcumae Radix was deeply and meticulously studied.</p><p><strong>Materials and methods: </strong>Curcumae Radix from different sources was collected, and its external morphology (volume and weight, color and smell), internal composition (extract, fingerprint and literature knowledge network diagram) and drug effect (efficacy of zebrafish on improving qi stagnation and blood stasis) were analyzed. The differences in composition and morphology of four kinds of Curcumae Radix were studied by HPLC fingerprint, chromaticity identification model, and BP-ANN classification model. Secondly, through the experiment of zebrafish \"qi stagnation and blood stasis\" model, the improvement effect of four kinds of turmeric on \"qi stagnation and blood stasis\" was explored. Finally, the relationship between the three dimensions of MCE of Curcumae Radix was discussed through Pearson analysis.</p><p><strong>Results: </strong>The pharmacodynamic experiment of zebrafish confirmed that all four kinds of Curcumae Radix showed the activity of treating \"qi stagnation and blood stasis\" and Curcuma phaeocaulis Val. (LSYJ) had the best effect comprehensively. And through Pearson analysis, it can be concluded that the smaller the external volume and weight of Curcumae Radix, the stronger the characteristic odor (W5S, W1W, and W2W), the closer the powder color is to red and yellow, the higher the internal extract (JCW) content of Curcumae Radix, the higher the contents of germacrone (JMT), furanodiene (FNEX), and β-elemene (β-LXX), and the better the efficacy of improving the disease of \"qi stagnation and blood stasis,\" among which JMT is the key drug component.</p><p><strong>Conclusion: </strong>The research demonstrates the value of a three-dimensional evaluation system for TCM quality assessment, providing insights into the relationship between morphology, composition, and efficacy. It offers a new perspective for quality control and evaluation of Curcumae Radix and provides a scientific basis for the clinical treatment of \"qi stagnation and blood stasis\" related diseases.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144192065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ATR-FTIR Coupled With Machine Learning Provides a Fast Method for Identifying and Distinguishing 55 Varieties of Fruit-Derived Medicinal Materials.","authors":"Wen-Jie Zhao, Ya-Ling An, Chun-Qian Song, Yu-Shi Huang, Li-Jie Zhang, Kang-Nan Liu, Zhen-Wei Li, Xiao-Kang Liu, Dai-di Zhang, De-An Guo","doi":"10.1002/pca.3545","DOIUrl":"https://doi.org/10.1002/pca.3545","url":null,"abstract":"<p><strong>Introduction: </strong>Fruit-derived medicinal materials (FDMM) are extensively utilized in daily life, yet the market is beset by substantial variety confusion, which undermines consumer rights and well-being. Consequently, accurate identification of these materials is essential for guaranteeing their quality, effectiveness, and safety.</p><p><strong>Objectives: </strong>This study aimed to combine attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and machine learning (ML) techniques to differentiate and identify 55 kinds of FDMM.</p><p><strong>Materials and methods: </strong>A total of 861 sample batches were collected, with 721 allocated for model establishment and 140 for independent validation. A PLS-DA model alongside nine machine learning algorithms-namely support vector machine (SVM), tree, K-nearest neighbor (KNN), discriminant, ensemble, support vector machine kernel (SVMK), logistic regression kernel (LRK), naive Bayes (NB), and neural network (NN)-were constructed. Considering both accuracy and computational efficiency, the optimal model was selected and evaluated in terms of its accuracy, precision, recall, and F1-score. The optimal model was further validated using 140 newly collected samples to ensure its long-term stability after several months.</p><p><strong>Results: </strong>Among the 10 classification models, the KNN model showed exceptional classification capability, with all evaluation metric exceeding 0.98. The KNN model was validated by the new 140 samples with a prediction accuracy of 85.7%, confirming its capability in distinguishing most FDMM.</p><p><strong>Conclusion: </strong>The application of ATR-FTIR technology combined with the robust classification capabilities of ML models enabled rapid and accurate differentiation and identification of 55 FDMM, which contributed to ensuring their quality.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144174526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted Neuroprotection in Sporadic Alzheimer's Disease: UPLC-ESI-MS/MS Profiling and Bilosome-Mediated Delivery of Crateva magna and Its Endophytic Fungal Extracts.","authors":"Aya N Talaat, Mohamed S Elnaggar, Nehal Ibrahim, Iriny M Ayoub, Haidy Abbas, Nesrine El Sayed, Rola M Labib, Abdel Nasser B Singab","doi":"10.1002/pca.3540","DOIUrl":"https://doi.org/10.1002/pca.3540","url":null,"abstract":"<p><strong>Introduction: </strong>Crateva magna (Cm) was utilized as a folkloric medicine against neurological disorders.</p><p><strong>Objectives: </strong>This study aimed to investigate the phytochemical profile of Cm leaf extract and its endophytic fungus, Nigrospora oryzae (No) extract. Additionally, the neuroprotective potential of their optimized bilosomes (BLs) will be assessed as an approach to Alzheimer's disease (AD) treatment.</p><p><strong>Materials and methods: </strong>UPLC-ESI-MS/MS chemical profiling was performed. In vitro anti-Alzheimer activity of Cm and No extracts was evaluated against AChE and BACE1 enzymes. Cm-BLs and No-BLs were prepared using the thin-film hydration technique. In vivo anti-Alzheimer potential was assessed in a streptozotocin (STZ)-induced sporadic AD mouse model. Behavioral assays, neurochemical assays, RT-PCR analysis, histopathological examination, and immunohistochemical analysis were performed.</p><p><strong>Results: </strong>Chemical profiling revealed diverse metabolites from various chemical classes. The major class identified in Cm extract was flavonoids, e.g., kaempferol-O-hexoside, whereas in No extract, it was alkaloids, e.g., phenazine carboxamide. The neuropathological markers (Aβ1-42, IL-6, and p-Tau protein) were reduced by ≈50% and 60% in mice receiving Cm-BLs and No-BLs, respectively, relative to the STZ group. Also, the BLs exhibited the greatest ability to downregulate the expression of p-JNK, p-P38, and p-ERK in the brain. Histopathological examination revealed that No-BLs showed the highest protection for the hippocampus and cerebral cortex regions. Also, it revealed a significantly decreased reaction for NFκB in cerebral cortex neurons.</p><p><strong>Conclusion: </strong>Cm-BLs and No-BLs exhibit considerable potential as novel adjuvant therapies for AD, utilizing natural bioactive compounds to improve the efficiency of targeted drug delivery and enhance therapeutic outcomes.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous Quantification of 22 Compounds in Talinum paniculatum Root and Proposal of Three Anti-Counterfeiting Quality Markers Using a Library-Aided UHPLC-Q-Orbitrap-MS/MS Method.","authors":"Jianwu Li, Hanxiao Chai, Xican Li, Shaoman Chen, Rongxin Cai, Lanying Wang, Xiaoqiong Xu, Zhenhui Chen","doi":"10.1002/pca.3543","DOIUrl":"https://doi.org/10.1002/pca.3543","url":null,"abstract":"<p><strong>Background: </strong>Talinum paniculatum root (pseudo ginseng in Chinese) is a widely distributed medicinal plant material in Asia, Africa, and America. However, its chemistry remains poorly understood nowadays.</p><p><strong>Methods: </strong>Fresh Talinum paniculatum root was treated for the preparation of a lyophilized aqueous extract. The extract thereafter was subjected to qualitative and quantitative analysis using the library-aided ultra-high-performance liquid chromatography-quadrupole-Exactive-Orbitrap-tandem mass spectrometry (UHPLC-Q-Exactive-Orbitrap-MS/MS) technology.</p><p><strong>Results: </strong>Based on MS/MS elucidation and comparison with the authentic standards in the library of UHPLC-Q-Exactive-Orbitrap-MS/MS apparatus, 22 compounds were accurately identified. Information retrieval suggested that 18 compounds belonged to \"unexcavated\" ones, which were found in the plant for the first time. Subsequently, all 22 compounds (including 18 \"unexcavated\" ones) were further quantified using the corresponding linear regression equations of authentic standards. All 22 compounds exhibited different contents from each other in the quantitative analysis. Particularly, synephrine had the highest content (8.159 ± 1.443 mg/g extract), and α-mangostin showed the lowest content (0.001 ± 0.000 mg/g extract), while chikusetsusaponin Iva displayed a moderate content (0.737 ± 0.274 mg/g extract).</p><p><strong>Conclusion: </strong>On the basis of these results and relevant principles, synephrine, N-trans-feruloyltyramine, and chikusetsusaponin Iva are recommended as the anti-counterfeiting Q-markers of T. paniculatum root. These findings will help to understand the substance basis of the traditional medicinal functions of T. paniculatum root and to find a suitable quality-control method.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Inflammatory Saponins From an Edible Aquatic Plant Nymphoides hydrophylla.","authors":"Ching-Yeu Chen, Tzu-Peng Cheng, Tsong-Long Hwang, Yong-Siang Hou, Limbadri Salendra, Tzi-Yi Ke, Yuan-Bin Cheng","doi":"10.1002/pca.3539","DOIUrl":"https://doi.org/10.1002/pca.3539","url":null,"abstract":"<p><strong>Introduction: </strong>The present investigation of Nymphoides hydrophylla is a Taiwan indigenous edible aquatic plant, belongs to the Menyanthaceae family, and has been used as a traditional food with potential medicinal values against fever, insect bites, ulcer, and skin diseases. Interestingly, we found that the plant extract showed a significant anti-inflammatory effect.</p><p><strong>Objectives: </strong>The aim is isolation, structure elucidation, and anti-inflammatory evaluation of secondary metabolites from N. hydrophylla.</p><p><strong>Material and methods: </strong>This study involves the bioactive isolation and structural studies of the pure compounds determined by NMR, UV, IR spectroscopies, and HRMS. The anti-inflammatory activity of the pure compounds was evaluated in vitro. In addition, isolates showed remarkable anti-inflammatory activities to regulate superoxide anion generation and elastase release in fMLF/CB-induced human neutrophils.</p><p><strong>Results: </strong>This study conducted 10 oleanane-type saponins with oligoglycoside linkage, including two new (1 and 2) identified from the butanol extract of N. hydrophylla. As a result, Compounds 1 and 2, representing new triterpene saponins, have not been previously reported. In vitro anti-inflammatory tests revealed that Compounds 1, 5-7, 9, and 10 showed significant anti-inflammatory effects by inhibiting superoxide anion generation with IC₅₀ values of 1.62, 0.92, 0.28, 2.69, 0.29, and 0.15 μM, respectively, whereas compounds 1, 5, 6, 9, and 10 showed potential inhibition on elastase release in human neutrophils, with IC₅₀ values of 0.19, 1.12, 0.70, 0.68, and 0.51 μM, respectively.</p><p><strong>Conclusion: </strong>Our findings suggested that the components of N. hydrophylla have the potential to develop anti-inflammatory agents.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhododendri Daurici Folium: An Overview of Studies on the Study of Herbal Medicine, Chemical Composition, Pharmacological Action, Clinical Application, and Quality Control.","authors":"Chenliang Li, Huixuan Tian, Wei Wu, Lingyang Kong, Jiao Xu, Lihong Zhang","doi":"10.1002/pca.3537","DOIUrl":"https://doi.org/10.1002/pca.3537","url":null,"abstract":"<p><p>The Rhododendri Daurici Folium (RDF) represents the desiccated foliage of Rhododendron dauricum L., belonging to the Rhododendron family. RDF abounds in diverse chemical elements, predominantly flavonoids, volatile oils, phenols, coumarin, and lignans. RDF functions as a cough and expectorant agent, primarily employed in the management of acute and chronic bronchitis and pharyngitis. RDF is also known for its pharmacological properties, including antibacterial, cardiotonic, antihypertensive, and anticancer properties. RDF is frequently utilized as a potent ingredient in Yingshanhong tablets, compound RDF syrup, XiaoKeChuan syrup, and various traditional Chinese medicinal concoctions, and it holds significant importance in clinical settings. This article's data were sourced from publications including PubMed, CNCK, SCI-HUB, Web of Science, Scopus, Chinese Pharmacopoeia, Chinese Dictionary of Traditional Chinese Medicine, Chinese Flora, and associated studies. The present document provides a comprehensive examination of the herbal properties, pharmacological effects, chemical makeup, clinical application, quality assurance, and safety assessments of RDF, serving as a guide for advancing and applying traditional Chinese medicine RDF.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Different Forms of Manganese Provide Distinctive Metabolomics Signatures and Bioactive Profiles in Artemisia annua.","authors":"Hajar Salehi, Abdolkarim Chehregani Rad, Luigi Lucini","doi":"10.1002/pca.3538","DOIUrl":"https://doi.org/10.1002/pca.3538","url":null,"abstract":"<p><strong>Introduction: </strong>Natural products are among the main ingredients of medicinal plants, and strategies that enhance their bioactive profile by elemental supplementation have emerged recently. Manganese is involved in various plant secondary metabolism pathways among different micronutrients.</p><p><strong>Objectives: </strong>This study investigated the effects of ionic (MnSO₄, MnCl₂), bulk (Mn₂O₃), and nano (Mn₂O₃-NP) manganese forms on Artemisia annua's secondary metabolism. It also studied the influence of the application method (seed priming vs. seed priming + foliar).</p><p><strong>Methods: </strong>For this purpose, untargeted UHPLC-QTOF-HRMS metabolomics was conducted.</p><p><strong>Results: </strong>The findings revealed that Mn form and application method significantly influenced the metabolomic profile and secondary metabolite composition of the leaves and inflorescences, regardless of tissue type. Metabolomic profiling using untargeted analysis and multivariate statistical tools (PCA, PLS-DA, and VIP scoring) showed significant variation in bioactive compound accumulation. Mn₂O₃ and MnCl₂ were most effective in enhancing nitrogen-containing compounds, phenylpropanoids, flavonoids, and terpenoids, possibly via ROS-mediated biosynthesis. Mn₂O₃ strongly increased lignans, while Mn₂O₃-NP showed the highest artemisinin accumulation (3.2-3.7 mg g^-1 FW) compared to MnCl₂ and Mn₂O₃ (0.2-1.7 mg g^-1 FW). Key pharmacological metabolites such as vincristine, Momilactone A, and terbinafine were identified by VIP2 analysis.</p><p><strong>Conclusion: </strong>Mn₂O₃-NP application through seed priming is a promising and cost-effective approach to modulate bioactive metabolite production in Artemisia annua.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive Apigenin-7-O-β-Glucoside and Rosmarinic Acid Molecules From Two Nepeta Species: Bioactivity-Guided Isolation, In Vitro Evaluations, Pharmacokinetic and In Silico Approaches as Metabolic Enzyme Inhibition Agents.","authors":"Mesut Gok, Yunus Basar, Semiha Yenigun, Yasar Ipek, Lutfi Behcet, Tevfik Ozen, Ibrahim Demirtas","doi":"10.1002/pca.3536","DOIUrl":"https://doi.org/10.1002/pca.3536","url":null,"abstract":"<p><strong>Introduction: </strong>In this study, apigenin-7-O-β-glucoside (AGL) was isolated from Nepeta nuda; also, rosmarinic acid (RA) was isolated from N. aristata and N. nuda.</p><p><strong>Objectives: </strong>The aim of this study is to investigate the enzyme inhibitory, DNA protective, and antibacterial effects of AGL and RA isolated from two Nepeta species.</p><p><strong>Material and methods: </strong>1D and 2D NMR spectra and an MS chromatogram were recorded to identify AGL and RA. The antibacterial and DNA protection activities, enzyme inhibition, and kinetics investigated of AGL and RA. Molecular interactions, molecular dynamics (MD) simulations, molecular mechanics Poisson-Boltzmann surface area (MM-PBSA) calculations, density functional theory (DFT), molecular electrostatic potential (MEP) analyses, and prediction of activity spectra for substances (PASS) predictions for RA and AGL were investigated for the first time to evaluate the activity results.</p><p><strong>Results: </strong>In this context, the inhibitory properties of AGL were higher in urease, α-amylase, and tyrosinase, whereas RA has a higher inhibitory activity on lipase, CA, and urease. In addition, AGL and RA showed effective antimicrobial activity against Staphylococcus aureus, while it also was effective DNA and deoxyribose protective activities. The stability of the complex formed by lipase, CA, and urease with RA and by tyrosinase and α-amylase with AGL was determined by MD simulations, and the energy results of RA were evaluated by MM-PBSA analysis. The DFT, MEP analysis, and PASS prediction showed that AGL and RA have a soft structure and can easily exchange electrons.</p><p><strong>Conclusion: </strong>According to the results obtained from the current study, AGL and RA were explored as a drug model.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144042679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Pectic Polysaccharide From Stems and Leaves of Panacis Quinquefolii Radix: Preparation, Structural Characteristic, and Inhibition of Cardiac Hypertrophy.","authors":"Minghui Zhang, Yu Xu, Lei Zhang, Peipei Wang","doi":"10.1002/pca.3535","DOIUrl":"https://doi.org/10.1002/pca.3535","url":null,"abstract":"<p><strong>Introduction: </strong>Panacis Quinquefolii Radix, a valued herb in traditional Chinese medicine, is frequently prescribed for its immunomodulatory effects and its potential to improve cardiovascular function. Recently, there is an increasing amount of research on ginseng polysaccharides, due to their variety of pharmacological activities, including anti-inflammatory, hepatoprotective, cardiovascular protective, and the improvement of intestinal function.</p><p><strong>Objective: </strong>This study investigates the efficacy of Panacis Quinquefolii Radix polysaccharides in alleviating cardiovascular diseases and to achieve the high-value utilization of ginseng by-products.</p><p><strong>Methodology: </strong>A pectic polysaccharide named XYSJY2-2 was extracted with hot water from stems and leaves of Panacis Quinquefolii Radix and further isolated by a DEAE Sepharose Fast Flow column and a Sephacry S-100 column. The structural characteristics of XYSJY2-2 was determined by monosaccharide composition, IR, NMR, and methylation analysis. The cardioprotective activity of this polysaccharide was evaluated in vitro and in vivo. With a relative molecular weight of 5.1 kDa, XYSJY2-2 is a type I rhamnogalacturonan featuring a core backbone made up of alternately linked rhamnose and galacturonic acid.</p><p><strong>Results: </strong>XYSJY2-2 effectively alleviated myocardial hypertrophy and remodeling induced by Ang II and TAC in vivo and in vitro, partly due to suppressing Nppa and Nppb expression.</p><p><strong>Conclusion: </strong>A pectic polysaccharide from stems and leaves of Panacis Quinquefolii Radix has the potential to alleviate cardiovascular disease.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual Assay Validation of Rosmarinus officinalis Extract as an Inhibitor of SARS-CoV-2 Spike Protein: Combining Pseudovirus Testing, Yeast Two-Hybrid, and UPLC-Q Exactive Orbitrap-MS Profiling.","authors":"Yujing Huang, Rufeng Luo, Chenjing Tian, Duntao Zu, Jianni Yang, Wenlin Chen, Dingqiang Huang, Siyan Duan, Shunxin Yan, Yujia Yuan, Shengrong Li, Haibo Zhou, Fulong Lin, Qinghui He, Junxia Zheng","doi":"10.1002/pca.3467","DOIUrl":"10.1002/pca.3467","url":null,"abstract":"<p><strong>Introduction: </strong>This study evaluates the effectiveness of Traditional Chinese Medicine (TCM) extracts in blocking the interaction between the SARS-CoV-2 Spike protein and human ACE2 receptor, utilizing a dual-method approach to explore the antiviral potential of natural compounds.</p><p><strong>Objectives: </strong>This work aims to evaluate the capability of TCM extracts in inhibiting the SARS-CoV-2 Spike protein and ACE2 receptor interaction using advanced biochemical assays.</p><p><strong>Methods: </strong>A dual-method screening approach was utilized, beginning with a pseudovirus assay to assess the inhibition capabilities of TCM extracts in vitro, followed by a split-ubiquitin yeast two-hybrid (Y2H) system to validate interactions in live cells. Active compounds were characterized and quantified using UPLC-Q-Exactive-Orbitrap-MS.</p><p><strong>Results: </strong>Among the 91 TCM extracts tested, Rosmarinus officinalis exhibited the most potent inhibition in both pseudovirus and Y2H assays, significantly reducing viral entry and disrupting the Spike-ACE2 interaction. Comprehensive chemical profiling via UPLC-Q-Exactive-Orbitrap-MS identified 132 compounds, including phenolics, flavonoids, and terpenoids.</p><p><strong>Conclusion: </strong>This research validates the use of TCM extracts in viral inhibition strategies, demonstrating the utility of integrating traditional remedies with modern scientific approaches to discover new therapeutic agents.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"776-792"},"PeriodicalIF":3.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}