{"title":"Fates of Diterpene Lactones in Storage Andrographis paniculata Extracts.","authors":"Thiwa Rattanaya, Chusanajit Chuangrattanawan, Krongkarn Chootip, Dumrongsak Pekthong, Anuchit Plubrukarn","doi":"10.1002/pca.3514","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Diterpene lactones in Andrographis paniculata are essential bioactive compounds that contribute to the herb's medicinal properties. The main diterpene lactones in A. paniculata are andrographolide and its derivatives. Spontaneous degradation of the diterpene leads to a short-term stability and casts a significant problem in storage and distribution of A. paniculata raw materials and products.</p><p><strong>Objective: </strong>To determine the stability of diterpene lactones in A. paniculata extracts and their transformation dynamics.</p><p><strong>Materials and methods: </strong>A <sup>1</sup>H NMR-based metabolomics workflow and HPLC-based quantitative determination were used to map the stability profiles in A. paniculata extract stored in the standard storage conditions over a 6-month period.</p><p><strong>Results: </strong>Qualitatively, NMR-based PCA detected both descending and ascending dynamics in transformation of diterpene lactones in A. paniculata extracts over a 6-month storage period. The contents of five lactone markers, compounds 1-5, in storage A. paniculata extracts were monitored to show that compounds 1 and 5 degraded with decelatory rates and presumably transformed via dehydration into compound 3, which otherwise increased also in a coherently decelatory manner. Lacking of the labile allylic hydroxyl group, compounds 2 and 4 were more stable and remained unchanged throughout the storage period.</p><p><strong>Conclusion: </strong>The short-term stability of A. paniculata extracts and related products was primarily attributed to the dehydration of 1 and it allylic hydroxy analogs, for example, 5, into a diene as in 3. Compound 3 is hence recommended as a plausible candidate as the stability and age marker of A. paniculata raw materials and products.</p>","PeriodicalId":20095,"journal":{"name":"Phytochemical Analysis","volume":" ","pages":"1367-1376"},"PeriodicalIF":3.0000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytochemical Analysis","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1002/pca.3514","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/2/11 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
Introduction: Diterpene lactones in Andrographis paniculata are essential bioactive compounds that contribute to the herb's medicinal properties. The main diterpene lactones in A. paniculata are andrographolide and its derivatives. Spontaneous degradation of the diterpene leads to a short-term stability and casts a significant problem in storage and distribution of A. paniculata raw materials and products.
Objective: To determine the stability of diterpene lactones in A. paniculata extracts and their transformation dynamics.
Materials and methods: A 1H NMR-based metabolomics workflow and HPLC-based quantitative determination were used to map the stability profiles in A. paniculata extract stored in the standard storage conditions over a 6-month period.
Results: Qualitatively, NMR-based PCA detected both descending and ascending dynamics in transformation of diterpene lactones in A. paniculata extracts over a 6-month storage period. The contents of five lactone markers, compounds 1-5, in storage A. paniculata extracts were monitored to show that compounds 1 and 5 degraded with decelatory rates and presumably transformed via dehydration into compound 3, which otherwise increased also in a coherently decelatory manner. Lacking of the labile allylic hydroxyl group, compounds 2 and 4 were more stable and remained unchanged throughout the storage period.
Conclusion: The short-term stability of A. paniculata extracts and related products was primarily attributed to the dehydration of 1 and it allylic hydroxy analogs, for example, 5, into a diene as in 3. Compound 3 is hence recommended as a plausible candidate as the stability and age marker of A. paniculata raw materials and products.
期刊介绍：
Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.
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