Quality Control of Shenqi Tongmai Oral Liquid Based on Quantitative Analysis of Multicomponents by Single Marker, Molecular Docking, and Multivariate Statistics.
Qian Li, Li Wang, Liuping Tan, Xiaojing Tao, Bei Zhang, Jingnan Pei, Qiuping Li
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引用次数: 0
Abstract
Introduction: Shenqi Tongmai oral liquid (SQTM) is famous for its remarkable effect in the treatment of cardiovascular diseases. However, the SQTM quality evaluation system has not been established.
Objective: The objective of this study is to establish a method for the determination of nine components of SQTM and to screen quality control indicators to comprehensively evaluate the quality of SQTM.
Methods: The fingerprints of SQTM were established, and the contents of nine components in 17 batches of SQTM were determined based on quantitative analysis of multicomponents by single marker (QAMS). The antioxidant activity of samples was determined by the DPPH method and hydroxyl method, and the correlation between the content of nine components and antioxidant activity was analyzed by gray relational analysis (GRA), bivariate correlation analysis (BCA), and partial least squares regression (PLSR). The antioxidant activity of the monomers was confirmed through molecular docking techniques and in vitro experiments.
Results: There was no significant difference in the content between the QAMS method and the external standard method (p > 0.05). The findings from multivariate statistics, molecular docking, and in vitro validation indicated that rosmarinic acid, luteolin, and protocatechualdehyde exhibited significant antioxidant activities, which were important pharmacodynamic components that exerted antioxidant effects and could serve as quality markers (Q-Markers).
Conclusion: The study elucidated the Q-Markers of SQTM and provided a relatively comprehensive approach for the assay of SQTM, which is a promising advance in the quality control of SQTM.
期刊介绍：
Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.
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