Pharmacy Education最新文献_第6页

Cost-effectiveness analysis of amlodipine and candesartan in the inpatient setting at Mataram University Hospital in Indonesia, 2021 2021 年印度尼西亚马打兰大学医院住院病人使用氨氯地平和坎地沙坦的成本效益分析

IF 0.5

Pharmacy Education Pub Date : 2024-05-01 DOI: 10.46542/pe.2024.243.228233

Nunung Uswatun Hasanah, Ni Made Amelia Ratnata Dewi, Yoga Dwi Saputra

{"title":"Cost-effectiveness analysis of amlodipine and candesartan in the inpatient setting at Mataram University Hospital in Indonesia, 2021","authors":"Nunung Uswatun Hasanah, Ni Made Amelia Ratnata Dewi, Yoga Dwi Saputra","doi":"10.46542/pe.2024.243.228233","DOIUrl":"https://doi.org/10.46542/pe.2024.243.228233","url":null,"abstract":"Background: Hypertension requires ongoing treatment, which could be costly. There are different single-drug therapy options available, such as amlodipine or candesartan, which have varying costs.\u0000Objective: This study aimed to analyse the cost-effectiveness of amlodipine and candesartan in hypertensive patients by determining the Average Cost Effectiveness Ratio (ACER) and Incremental Cost Effectiveness Ratio (ICER) ratios from a hospital perspective.\u0000Method: This study used probability sampling of retrospective data from 2021 for the analysis. All inpatients who were given single therapy with amlodipine of 10 mg or candesartan of 16 mg were included. Direct medical costs were collected, including medicine and room costs, doctor visits, medical procedures, labour and administration. The effectiveness of the therapy was measured by blood pressure reduction from each therapy. ACER and ICER analyses were conducted to determine the most cost-effective therapy.\u0000Result: A total of 18 samples met the inclusion criteria, 14 patients received amlodipine therapy, and four received candesartan therapy. The results showed that the cost-effectiveness of antihypertensive therapy with amlodipine and candesartan, as measured by ACER, was IDR 74,851.15 and IDR 87,809.25, respectively. The ICER value obtained was IDR 362,768.\u0000Conclusion: The results may suggest that amlodipine is more cost-effective than candesartan.","PeriodicalId":19944,"journal":{"name":"Pharmacy Education","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141046830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and physicochemical characterization of nanostructured lipid carriers for entrapment of vitamin D3 prepared at different lipid ratios 以不同脂质比例制备的用于包埋维生素 D3 的纳米结构脂质载体的开发和理化特性分析

IF 0.5

Pharmacy Education Pub Date : 2024-05-01 DOI: 10.46542/pe.2024.243.204210

Ida Kristianingsih, E. Hendradi, Siswandono Siswodihardjo, M. Yuwono

{"title":"Development and physicochemical characterization of nanostructured lipid carriers for entrapment of vitamin D3 prepared at different lipid ratios","authors":"Ida Kristianingsih, E. Hendradi, Siswandono Siswodihardjo, M. Yuwono","doi":"10.46542/pe.2024.243.204210","DOIUrl":"https://doi.org/10.46542/pe.2024.243.204210","url":null,"abstract":"Background: Vitamin D3 plays a role in immunity, especially in the body's regulation of the inflammatory response. The limitations of vitamin D3 are poor bioavailability and water-insoluble. Nanostructured Lipid Carriers (NLC) can increase the solubility of water-insoluble active ingredients.\u0000Objective: To develop NLC as a delivery agent of vitamin D3 for topical application.\u0000Method: NLC was created using a high-shear homogenisation method with various lipid ratios. NLC uses solid lipids (Monostearin) and liquid lipids (Miglyol 812) with variations in the ratios of F1 (7:3), F2 (8:2) and F3 (9:1). NLC is expected to be able to protect the active ingredients in the lipid matrix so that the stability of the active ingredients increases. Characterisation was performed in organoleptic, pH, particle size, viscosity, zeta potential, and entrapment efficiency.\u0000Result: Vitamin D3 NLC system had good characteristics: pH suitable for topical use, particle size < 600nm, PI < 0.5, Zeta potential -26.80 to -33.76. F1 resulted in the highest entrapment efficiency of 82.18%.\u0000Conclusion: Vitamin D3 NLC systems with different lipid concentration ratios affect the physical and chemical characteristics. Increasing the liquid lipid content in the Vit D3 NLC system can reduce particle size and viscosity and increase entrapment efficiency.","PeriodicalId":19944,"journal":{"name":"Pharmacy Education","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141052646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pinostrobin and its derivatives as novel anti-breast cancer agents against human oestrogen receptor alpha: In silico studies of ADMET, docking, and molecular dynamics Pinostrobin 及其衍生物作为抗人类雌激素受体 alpha 的新型抗乳腺癌药物：ADMET、对接和分子动力学的硅学研究

IF 0.5

Pharmacy Education Pub Date : 2024-05-01 DOI: 10.46542/pe.2024.243.5156

Delis Susilawati, T. Widiandani, Siswandono Siswodihardjo, Suzana Suzana, Bambang Tri Purwanto

{"title":"Pinostrobin and its derivatives as novel anti-breast cancer agents against human oestrogen receptor alpha: In silico studies of ADMET, docking, and molecular dynamics","authors":"Delis Susilawati, T. Widiandani, Siswandono Siswodihardjo, Suzana Suzana, Bambang Tri Purwanto","doi":"10.46542/pe.2024.243.5156","DOIUrl":"https://doi.org/10.46542/pe.2024.243.5156","url":null,"abstract":"Background: Breast cancer is the main cause of cancer death among women. The issue is more complex due to the side effects and resistance of the currently used breast cancer drugs. Pinostrobin, a compound found in Boesenbergia pandurata, has anticancer activity. Modifying the structure of pinostrobin can improve drug bioavailability, reduce toxicity, and work more selectively.\u0000Objective: This study aimed to predict the potentials of pinostrobin and its derivatives as anti-breast cancer agents against human oestrogen receptors by an in silico approach.\u0000Methods: The pkCSM was utilised to predict the ADMET characteristics. The interaction of ligands with the binding site of the human oestrogen receptor alpha with PDB ID 3ERT was used for molecular docking using MOE, and AMBER was then used for molecular dynamic simulation.\u0000Results: Pinostrobin pentanoate had good pharmacokinetic properties and was neither mutagenic nor hepatotoxic. Based on molecular docking, it was more potent compared to pinostrobin, with binding free energy values of -7.849 kcal/mol. Furthermore, in the interaction stability evaluation using 100 ns molecular dynamic simulation by the MM-GBSA calculation method, pinostrobin pentanoate had a stable interaction with a total bond energy value of -9.943 kcal/mol.\u0000Conclusion: Pinostrobin pentanoate has the potential as an anti-breast cancer alternative through the human oestrogen receptor alpha.","PeriodicalId":19944,"journal":{"name":"Pharmacy Education","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141039185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of phagocytic index and haematological parameters of Sida rhombifolia extracts in mice as immunomodulator 评价作为免疫调节剂的豨莶草提取物在小鼠体内的吞噬指数和血液学参数

IF 0.5

Pharmacy Education Pub Date : 2024-05-01 DOI: 10.46542/pe.2024.243.95100

T. Wahyuni, Widya Wasityastuti, D. Nugrahaningsih, Suratno Lulut Ratnoglik, Laura Yamani, Ahmad El-Shamy, A. Widyawaruyanti

{"title":"Evaluation of phagocytic index and haematological parameters of Sida rhombifolia extracts in mice as immunomodulator","authors":"T. Wahyuni, Widya Wasityastuti, D. Nugrahaningsih, Suratno Lulut Ratnoglik, Laura Yamani, Ahmad El-Shamy, A. Widyawaruyanti","doi":"10.46542/pe.2024.243.95100","DOIUrl":"https://doi.org/10.46542/pe.2024.243.95100","url":null,"abstract":"Background: Sida rhombifolia was reported to have potential anti-hepatitis C virus activity. Anti-viral activities of plants were also reported to modulate immunity.\u0000Objective: This research was conducted by testing the immunomodulating activities of Sida rhombifolia extract.\u0000Method: This study evaluated cell viability in HepG2 culture cells to determine the 50% of cell viability. The Immunomodulatory activity was evaluated by examining the phagocytic index value and haematology profile.\u0000Result: The results showed that the IC50 Sida rhombifolia was 32.5 μg/mL, while the IC50 of CCl4 as negative control was 16.68 μg/mL. Moreover, 100% of cell viability was observed at ½ IC50 and ¼ IC50 of Sida rhombifolia, indicating no toxic effect from the extract. Immunomodulatory assay found that Sida rhombifolia extract demonstrated an immunostimulant effect. The phagocytic index value of Sida rhombifolia was higher than that of the standard drug (a commercial immunostimulant product). Moreover, the haematology evaluation demonstrated no negative effect on haemoglobin, hematocrit and erythrocyte, which showed no difference in values compared to the normal control and the immunostimulant product.\u0000Conclusion: Sida rhombifolia plant extract has the potential as an immunomodulator candidate for treating hepatitis.","PeriodicalId":19944,"journal":{"name":"Pharmacy Education","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141048412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perception of online interprofessional education among pharmacy and medical students in tertiary university 高等院校药学和医学专业学生对在线跨专业教育的看法

IF 0.5

Pharmacy Education Pub Date : 2024-05-01 DOI: 10.46542/pe.2024.243.4550

Ganesh Sritheran Paneerselvam, Muhamad Junaid Farrukh, Long Chiau Ming, Andi Hermansyah

{"title":"Perception of online interprofessional education among pharmacy and medical students in tertiary university","authors":"Ganesh Sritheran Paneerselvam, Muhamad Junaid Farrukh, Long Chiau Ming, Andi Hermansyah","doi":"10.46542/pe.2024.243.4550","DOIUrl":"https://doi.org/10.46542/pe.2024.243.4550","url":null,"abstract":"Background: The COVID-19 crisis impacted the educational system worldwide, which shifted from face-to-face sessions to online learning modes. However, online delivery should not jeopardise the outcome of students learning from interprofessional education (IPE) and should integrate students from different professional backgrounds closer.\u0000Objective: To identify the perception of medical and pharmacy students towards online IPE activity at Taylor’s University, Malaysia.\u0000Method: An online cross-sectional questionnaire was distributed to second and fourth-year students from the medical and pharmacy programmes. Students were considered to have a positive perception if their total score was above the median score.\u0000Result: More than half of the students (53.6%) showed positive perceptions towards the online IPE programme. Medical students had the highest percentage of students (54.9%) showing greater positive perceptions compared to pharmacy students (52.7%). Both health sciences students possessed the highest score on all the SPICE instrument’s domains.\u0000Conclusion: This discovery offers valuable insights into the interprofessional competency of students engaged in online IPE activities at their current academic level. It underscores the imperative of integrating online IPE activities into the curriculum design to equip students with the competence necessary for effective collaboration within an interprofessional team. ","PeriodicalId":19944,"journal":{"name":"Pharmacy Education","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141044820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design of acyl salicylic acid derivates as COX-1 inhibitors using QSAR approach, molecular docking and QSPR analysis 利用 QSAR 方法、分子对接和 QSPR 分析设计作为 COX-1 抑制剂的酰基水杨酸衍生物

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Pharmacy Education Pub Date : 2024-05-01 DOI: 10.46542/pe.2024.243.8894

N. Diyah, Dhea Ananda Ainurrizma, Denayu Pebrianti

{"title":"Design of acyl salicylic acid derivates as COX-1 inhibitors using QSAR approach, molecular docking and QSPR analysis","authors":"N. Diyah, Dhea Ananda Ainurrizma, Denayu Pebrianti","doi":"10.46542/pe.2024.243.8894","DOIUrl":"https://doi.org/10.46542/pe.2024.243.8894","url":null,"abstract":"Background: Acetylsalicylic acid (aspirin), widely used as an antiplatelet agent, is more likely to inhibit COX-1. Along with discovering the cardioprotective role of COX-1 in controlling platelet aggregation, it is important to develop a selective COX-1 inhibitor.\u0000Objective: This study aims to design acyl salicylic acid derivatives intended as COX-1 inhibitors.\u0000Method: Fourteen derivatives (AcS1-14) were subjected to a quantitative structure-activity relationship (QSAR) study, and 31 QSAR models were obtained using multiple linear regression (MLR) analysis. Molecular docking was performed on COX-1 (PDB. 1PTH) using the Molecular Orbital Environment (MOE) program ver2022.02, and QSPR analysis was conducted to ascertain the contribution of physicochemical descriptors to the free energy score (S) of ligand-receptor complexes.\u0000Results: The QSAR-Hansch model predicted hydrophobicity (LogP) and molecular energy (Etotal) and contributed to pain inhibitory action. All derivatives displayed higher in silico affinity than aspirin (S= -4.33±0.00 kcal/mol), and compound AcS7 afforded the highest (S= -5.32 kcal/mol). In QSPR, Etotal also revealed a positive contribution to the affinity. AcS1, AcS2, AcS5, AcS7, and AcS8 expressed higher drug-like properties than aspirin.\u0000Conclusion: Derivatives with optimum hydrophobicity and high energy would generate potent COX-1 inhibition. The five selected compounds were recommended to be developed as drug candidates for COX-1 inhibitors.","PeriodicalId":19944,"journal":{"name":"Pharmacy Education","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141058557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Implementation of a pharmacovigilance system to detect adverse events and improve medication appropriateness in a hospital in Indonesia 印度尼西亚一家医院实施药物警戒系统以检测不良事件并提高用药适当性

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Pharmacy Education Pub Date : 2024-05-01 DOI: 10.46542/pe.2024.243.711

D. Atmaja, Yulistiani Yulistiani, S. Suharjono, E. Zairina

{"title":"Implementation of a pharmacovigilance system to detect adverse events and improve medication appropriateness in a hospital in Indonesia","authors":"D. Atmaja, Yulistiani Yulistiani, S. Suharjono, E. Zairina","doi":"10.46542/pe.2024.243.711","DOIUrl":"https://doi.org/10.46542/pe.2024.243.711","url":null,"abstract":"Background: The growing elderly population has led to an increased occurrence of adverse drug reactions (ADRs) due to the various drugs they are prescribed, posing the problem of potential drug interactions. The application of causality instruments combined with trigger tools and the competency of healthcare professionals has yielded better results in detecting ADRs.\u0000Objective: To evaluate the implementation of a pharmacovigilance system to detect ADRs, potentially inappropriate medications, and potential prescription omissions.\u0000Methods: An observational, descriptive, prospective study was conducted over six weeks at one of the largest hospitals in Indonesia. The pharmacist and the physicians collaborated in reviewing patient medication records using the pharmacovigilance system.\u0000Results: The study enrolled 144 patients. The 163 positive triggers identified helped detect 28 Adverse Drug Events (ADEs) caused by at least one high-alert medication. These ADEs occurred in 25 patients. Additionally, 62 potentially inappropriate medications and 41 potential prescription omissions were detected.\u0000Conclusion: The pharmacovigilance system could reduce the frequency of ADEs caused by medications used in elderly patients.","PeriodicalId":19944,"journal":{"name":"Pharmacy Education","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141026224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An in silico study of the effects of chemical compounds in Petiveria alliacea leaf extract on inflammatory mediators 关于小苍兰叶提取物中的化学物质对炎症介质影响的硅学研究

IF 0.5

Pharmacy Education Pub Date : 2024-05-01 DOI: 10.46542/pe.2024.243.153158

N. Fatimah, Arifa Mustika, S. Sudjarwo, Ahmad Cholifa Fahruddin, Lutfiah Anjarwati

{"title":"An in silico study of the effects of chemical compounds in Petiveria alliacea leaf extract on inflammatory mediators","authors":"N. Fatimah, Arifa Mustika, S. Sudjarwo, Ahmad Cholifa Fahruddin, Lutfiah Anjarwati","doi":"10.46542/pe.2024.243.153158","DOIUrl":"https://doi.org/10.46542/pe.2024.243.153158","url":null,"abstract":"Background: Petiveria alliacea (P. alliacea) is a botanical species renowned for its bioactive compounds and is utilised for medicinal purposes worldwide. In Southwestern Nigeria, P. alliacea finds common application in herbal medicine to address diverse ailments, including diabetes due to chronic inflammation.\u0000Objective: This study investigates the drug-like molecular properties of chemical compounds in P. alliacea, targeting the interleukin one receptor (IL1R) and Tumor Necrosis Factor-alpha receptor (TNFAR).\u0000Method: The target binding of the P. alliacea chemical compounds was evaluated through drug-likeness tests on the SCFBIO server. All compounds found in P. alliacea adhere to Lipinski’s Rule of Five, classifying them as drug-like molecules. Employing molecular docking simulations on PyRx v9.9.0, the interaction dynamics between P. alliacea ligands and IL1R and TNFAR were simulated.\u0000Result: Among the compounds found in P. alliacea, namely astilbin and isoarborinol, emerge as potential candidates for IL1R and TNFAR protein inhibitors due to their notably elevated negative binding affinity values and involve Van der Waals, hydrogen and alkyl bond interactions. Then, a response was elicited that was marked by diminished oxidative stress and anti-inflammatory activity.\u0000Conclusion: P. alliacea has the potential to inhibit proinflammatory proteins, such as IL1R and TNFAR, due to its content, namely astilbin and isoarborinol.","PeriodicalId":19944,"journal":{"name":"Pharmacy Education","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141135557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The pH-solubility profiles of levofloxacin hemihydrate and ciprofloxacin lactate 半水左氧氟沙星和乳酸环丙沙星的 pH 溶解度曲线

IF 0.5

Pharmacy Education Pub Date : 2024-05-01 DOI: 10.46542/pe.2024.243.105110

Dewi Isadiartuti, Retno Sari, Dini Retnowati, Risma Dama Yanti, Violyta Ade Gunawan, Oki Yudiswara

{"title":"The pH-solubility profiles of levofloxacin hemihydrate and ciprofloxacin lactate","authors":"Dewi Isadiartuti, Retno Sari, Dini Retnowati, Risma Dama Yanti, Violyta Ade Gunawan, Oki Yudiswara","doi":"10.46542/pe.2024.243.105110","DOIUrl":"https://doi.org/10.46542/pe.2024.243.105110","url":null,"abstract":"Background: Levofloxacin and ciprofloxacin are popular fluoroquinolone antibiotics that offer diverse therapeutic potentials due to their broad-spectrum activity. Levofloxacin and ciprofloxacin, commonly used as active pharmaceutical ingredients (API) in parenteral form, are amphoteric compounds with pH-sensitive solubility. This characteristic may affect the safety and efficacy of levofloxacin and ciprofloxacin, especially in the parenteral dosage forms.\u0000Objective: This study aims to assess the solubility of levofloxacin hemihydrate and ciprofloxacin lactate in buffer solutions with pH ranging from 3.0 to 8.0.\u0000Method: The solubility test was conducted in a 0.02 M buffer pH solution featuring an ionic strength (µ) of 0.2. After weighing several API samples, ultrasonication was carried out for 15 minutes. The solution was agitated at 150 rpm and 30 ± 0.5°C until it reached equilibrium (four hours). The API concentration was observed using a UV-Vis spectrophotometer.\u0000Result: The solubility ranges of levofloxacin hemihydrate and ciprofloxacin lactate were 44.39 to 70.66 mg/mL and 0.23 to 243.00 mg/mL, respectively, within the experimental pH range.\u0000Conclusion: This study concluded that levofloxacin hemihydrate and ciprofloxacin lactate solubility were increased due to decreasing pH medium. ","PeriodicalId":19944,"journal":{"name":"Pharmacy Education","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141022851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zebrafish as a model for the study of wound healing in hyperglycemia 将斑马鱼作为研究高血糖状态下伤口愈合的模型

IF 0.5

Pharmacy Education Pub Date : 2024-05-01 DOI: 10.46542/pe.2024.243.111115

Lia Nurkhasanah, Farida Hayati, R. Istikharah

{"title":"Zebrafish as a model for the study of wound healing in hyperglycemia","authors":"Lia Nurkhasanah, Farida Hayati, R. Istikharah","doi":"10.46542/pe.2024.243.111115","DOIUrl":"https://doi.org/10.46542/pe.2024.243.111115","url":null,"abstract":"Background: For preclinical studies of diabetic wound therapy, zebrafish have many orthologous signalling pathways that play essential roles in wound healing and regeneration.\u0000Objectives: This study compares the expression of four essential wound-healing genes and caudal fin regeneration in hyperglycemic zebrafish (Danio rerio) to normal zebrafish.\u0000Methods: Hyperglycemia was induced by using the intraperitoneal injection method with 350 mg/BW streptozotocin on days one, three, and five. The regeneration of the zebrafish's caudal fin was observed on day 5 after amputation using a stereomicroscope, followed by sampling of the blastema to analyse gene expression. Caudal fin regeneration was analysed using Zeiss Zen 3.3 blue documentation; blood glucose levels were measured using a glucometer; and relative gene expression analysis of sonic hedgehog (shh), insulin-like growth factor 2a (igf2a), bone morphogenetic protein 2b (bmp2b), collagen 1a2 (col1a2) was performed by qRT-PCR method.\u0000Results: The glucose level in the hyperglycemia group was 203.2 mg/dL, and that in the normal group was 59.5 mg/dL. The authors found that igf2a, shh, bmp2b, and col1a2 expression were all downregulated in the hyperglycemia group and decreased in caudal fin regeneration.\u0000Conclusion: This novel adult zebrafish model of hyperglycemia significantly impairs gene expression and caudal fin regeneration.","PeriodicalId":19944,"journal":{"name":"Pharmacy Education","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141042641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0