Pathobiology最新文献_第6页

Artificial Intelligence in Bone Marrow Histological Diagnostics: Potential Applications and Challenges. 人工智能在骨髓组织学诊断中的应用：潜在应用与挑战。

IF 3.5 4区医学

Pathobiology Pub Date : 2024-01-01 Epub Date: 2023-02-15 DOI: 10.1159/000529701

Leander van Eekelen, Geert Litjens, Konnie M Hebeda

引用次数: 0

Myeloid Proliferations Associated with Down Syndrome: Clinicopathologic Characteristics of Forty Cases from Five Large Academic Institutions. 与唐氏综合征相关的骨髓增生：来自五所大型学术机构的四十例病例的临床病理特征。

IF 5 4区医学

Pathobiology Pub Date : 2024-01-01 Epub Date: 2023-03-30 DOI: 10.1159/000530431

Tayler A van den Akker, Yen-Chun Liu, Huifei Liu, Jennifer Chapman, Jennifer M Levine, Olga K Weinberg, Julia T Geyer

{"title":"Myeloid Proliferations Associated with Down Syndrome: Clinicopathologic Characteristics of Forty Cases from Five Large Academic Institutions.","authors":"Tayler A van den Akker, Yen-Chun Liu, Huifei Liu, Jennifer Chapman, Jennifer M Levine, Olga K Weinberg, Julia T Geyer","doi":"10.1159/000530431","DOIUrl":"10.1159/000530431","url":null,"abstract":"Introduction: The incidence of myelodysplastic syndrome and acute myeloid leukemia is significantly increased in children with Down syndrome (DS). Within the revised 2016 WHO edition, these entities are jointly classified as myeloid leukemia associated with DS (ML-DS). Additionally, infants with DS may develop transient abnormal myelopoiesis (TAM) which is histomorphologically similar to ML-DS. While TAM is self-limiting, it is associated with an increased risk of subsequently developing ML-DS. Differentiating TAM and ML-DS is challenging but clinically critical.Methods: We performed a retrospective review of ML-DS and TAM cases collected from five large academic institutions in the USA. We assessed clinical, pathological, immunophenotypical, and molecular features to identify differentiating criteria.Results: Forty cases were identified: 28 ML-DS and 12 TAM. Several features were diagnostically distinct, including younger age in TAM (p < 0.05), as well as presentation with clinically significant anemia and thrombocytopenia in ML-DS (p < 0.001). Dyserythropoiesis was unique to ML-DS, as well as structural cytogenetic abnormalities aside from the constitutional trisomy 21. Immunophenotypic characteristics of TAM and ML-DS were indistinguishable, including the aberrant expression of CD7 and CD56 by the myeloid blasts.Discussion: The findings of the study confirm marked biological similarities between TAM and ML-DS. At the same time, several significant clinical, morphological, and genetic differences were observed between TAM and ML-DS. The clinical approach and the differential diagnosis between these entities are discussed in detail.","PeriodicalId":19805,"journal":{"name":"Pathobiology","volume":" ","pages":"89-98"},"PeriodicalIF":5.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10857798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9227429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinically Applicable Pan-Origin Cancer Detection for Lymph Nodes via Artificial Intelligence-Based Pathology. 通过基于人工智能的病理学对淋巴结进行临床适用的泛源癌症检测。

IF 3.5 4区医学

Pathobiology Pub Date : 2024-01-01 Epub Date: 2024-05-08 DOI: 10.1159/000539010

Yi Pan, Hongtian Dai, Shuhao Wang, Lang Wang, Qiting Li, Wenmiao Wang, Jiangtao Li, Dan Qi, Zhaoyang Yang, Jia Jia, Yaxi Wang, Qing Fang, Lin Li, Weixun Zhou, Zhigang Song, Shuangmei Zou

{"title":"Clinically Applicable Pan-Origin Cancer Detection for Lymph Nodes via Artificial Intelligence-Based Pathology.","authors":"Yi Pan, Hongtian Dai, Shuhao Wang, Lang Wang, Qiting Li, Wenmiao Wang, Jiangtao Li, Dan Qi, Zhaoyang Yang, Jia Jia, Yaxi Wang, Qing Fang, Lin Li, Weixun Zhou, Zhigang Song, Shuangmei Zou","doi":"10.1159/000539010","DOIUrl":"10.1159/000539010","url":null,"abstract":"Introduction: Lymph node metastasis is one of the most common ways of tumour metastasis. The presence or absence of lymph node involvement influences the cancer's stage, therapy, and prognosis. The integration of artificial intelligence systems in the histopathological diagnosis of lymph nodes after surgery is urgent.Methods: Here, we propose a pan-origin lymph node cancer metastasis detection system. The system is trained by over 700 whole-slide images (WSIs) and is composed of two deep learning models to locate the lymph nodes and detect cancers.Results: It achieved an area under the receiver operating characteristic curve (AUC) of 0.958, with a 95.2% sensitivity and 72.2% specificity, on 1,402 WSIs from 49 organs at the National Cancer Center, China. Moreover, we demonstrated that the system could perform robustly with 1,051 WSIs from 52 organs from another medical centre, with an AUC of 0.925.Conclusion: Our research represents a step forward in a pan-origin lymph node metastasis detection system, providing accurate pathological guidance by reducing the probability of missed diagnosis in routine clinical practice.","PeriodicalId":19805,"journal":{"name":"Pathobiology","volume":" ","pages":"345-358"},"PeriodicalIF":3.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140892189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IQ Motif Containing GTPase-Activating Protein 3 Is Associated with Cancer Stemness and Survival in Pancreatic Ductal Adenocarcinoma. 含 IQ motif 的 GTPase 活化蛋白 3 与胰腺导管腺癌的癌症干性和存活率有关。

IF 3.5 4区医学

Pathobiology Pub Date : 2024-01-01 Epub Date: 2023-12-16 DOI: 10.1159/000535542

Aya Kido, Akira Ishikawa, Takafumi Fukui, Narutaka Katsuya, Kazuya Kuraoka, Kazuhiro Sentani, Sho Tazuma, Takeshi Sudo, Masahiro Serikawa, Shiro Oka, Naohide Oue, Wataru Yasui

{"title":"IQ Motif Containing GTPase-Activating Protein 3 Is Associated with Cancer Stemness and Survival in Pancreatic Ductal Adenocarcinoma.","authors":"Aya Kido, Akira Ishikawa, Takafumi Fukui, Narutaka Katsuya, Kazuya Kuraoka, Kazuhiro Sentani, Sho Tazuma, Takeshi Sudo, Masahiro Serikawa, Shiro Oka, Naohide Oue, Wataru Yasui","doi":"10.1159/000535542","DOIUrl":"10.1159/000535542","url":null,"abstract":"Introduction: Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal types of malignancy, with poor prognosis and rising incidence. IQ motif containing GTPase-activating protein 3 (IQGAP3) is a member of the IQGAPs family of scaffolding proteins that govern multiple cellular activities like cytoskeletal remodeling and cellular signal transduction. This study aimed to analyze the expression and biological function of IQGAP3 in PDAC.Methods: We analyzed IQGAP3 expression in 81 PDAC samples by immunohistochemistry. RNA interference was used to inhibit IQGAP3 expression in PDAC cell lines.Results: Immunohistochemical analysis of IQGAP3 showed that 54.3% of PDACs were positive for cytoplasmic expression of IQGAP3, with no expression found in non-neoplastic tissue. Furthermore, IQGAP3 expression was an independent poor prognostic factor in our immunostaining-based studies and analyses of public databases. Our cohort and the Cancer Genome Atlas database indicated that IQGAP3 is co-localized with kinesin family member C1 (KIFC1), which we previously reported as a cancer stem cell-associated protein. IQGAP3 small interfering RNA treatment decreased PDAC cell proliferation and spheroid colony formation via ERK and AKT pathways.Discussion/conclusion: These results suggest that IQGAP3, a transmembrane protein, is involved in survival and stemness and may be a promising new therapeutic target for PDAC.","PeriodicalId":19805,"journal":{"name":"Pathobiology","volume":" ","pages":"268-278"},"PeriodicalIF":3.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11309048/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138801012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reconceiving Perineural Invasion in Cutaneous Squamous Cell Carcinoma: From Biological to Histopathological Assessment. 重新认识皮肤鳞状细胞癌的神经周围侵犯：从生物学评估到组织病理学评估。

IF 3.5 4区医学

Pathobiology Pub Date : 2024-01-01 Epub Date: 2024-07-24 DOI: 10.1159/000539484

Filippo Nozzoli, Romina Nassini, Francesco De Logu, Martina Catalano, Giandomenico Roviello, Daniela Massi

{"title":"Reconceiving Perineural Invasion in Cutaneous Squamous Cell Carcinoma: From Biological to Histopathological Assessment.","authors":"Filippo Nozzoli, Romina Nassini, Francesco De Logu, Martina Catalano, Giandomenico Roviello, Daniela Massi","doi":"10.1159/000539484","DOIUrl":"10.1159/000539484","url":null,"abstract":"Background: Perineural invasion (PNI) is a complex molecular process histologically represented by the presence of tumor cells within the peripheral nerve sheath and defined when infiltration into the 3 nerve sheath layers can be clearly identified. Several molecular pathways have been implicated in cSCC. PNI is a well-recognized risk factor in cutaneous squamous cell carcinoma (cSCC) and its accurate assessment represents a challenging field in pathology daily practice.Summary: As a highly intricate and dynamic process, PNI involves a contingent on bidirectional signaling interactions between the tumor and various nerve components, such as Schwann cells and neurons. The current staging systems recommend the identification of PNI as a dichotomous variable (presence vs. absence) to identify a subgroup of high-risk patients. However, recent further insights revealed that the evaluation of morphological PNI-related features in cSCC may enhance the prognostic stratification of patients and may optimize the current staging guidelines for recurrence risk assessment and improvement of patient selection for postoperative adjuvant treatments. Furthermore, recent emerging biomarkers could redefine early PNI detection.Key messages: This review provides updated insights into cSCC with PNI, focusing on molecular and cellular pathogenic processes, and aims to increase knowledge on prognostic relevant PNI-related histological features.","PeriodicalId":19805,"journal":{"name":"Pathobiology","volume":" ","pages":"442-454"},"PeriodicalIF":3.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11614312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current challenges in the characterization of myeloid neoplasms 髓系肿瘤特征描述目前面临的挑战

IF 5 4区医学

Pathobiology Pub Date : 2023-12-22 DOI: 10.1159/000535852

Julia T. Geyer

引用次数: 0

Contents 2023 Vol. 90 目录 2023 卷 90

IF 5 4区医学

Pathobiology Pub Date : 2023-12-01 DOI: 10.1159/000535593

Ettenheim Stückle Druck, Fernando C. Schmitt, Pedro L. Fernández, Alexander Katalinic – Institut, Jose A. Lorente, Antonio Marra – Memorial, Stefano A. Pileri – European, Q. T. Pham, Hiroshima, Ho Chi, Minh, E. Dorado-Fernández, Madrid Aso-Escario, J. Aso-Vizán, A. Zaragoza, I. Ramírez-González, Guadalajara, Madrid, M. F. Carrillo-Rodríguez, D. Cáceres-Monllor, J. Murillo-González, M. J. Brito, Almada, Lisboa, E. M. Halushka, M.K.

引用次数: 0

Essential Roles of TDO2 in Gastric Cancer: TDO2 Is Associated with Cancer Progression, Patient Survival, PD-L1 Expression, and Cancer Stem Cells. TDO2在胃癌中的重要作用:TDO2与癌症进展、患者生存、PD-L1表达和癌症干细胞相关。

IF 5 4区医学

Pathobiology Pub Date : 2023-01-01 DOI: 10.1159/000523750

Quoc Thang Pham, Daiki Taniyama, Shintaro Akabane, Tsuyoshi Takashima, Ryota Maruyama, Yohei Sekino, Kazuhiro Sentani, Wataru Yasui, Naohide Oue

{"title":"Essential Roles of TDO2 in Gastric Cancer: TDO2 Is Associated with Cancer Progression, Patient Survival, PD-L1 Expression, and Cancer Stem Cells.","authors":"Quoc Thang Pham, Daiki Taniyama, Shintaro Akabane, Tsuyoshi Takashima, Ryota Maruyama, Yohei Sekino, Kazuhiro Sentani, Wataru Yasui, Naohide Oue","doi":"10.1159/000523750","DOIUrl":"https://doi.org/10.1159/000523750","url":null,"abstract":"Introduction: Tryptophan metabolism has been shown to be involved in tumor development. Two main tryptophan-degrading enzymes, tryptophan 2,3-dioxygenase (TDO2) and indoleamine 2,3-dioxygenase 1 (IDO1), may potently promote cancer cell survival and distant metastasis in diverse types of cancer, such as lung and breast cancer. IDO1 overexpression is an independent prognosticator in gastric cancer (GC). This work aimed to uncover the expression of TDO2 and its clinicopathologic significance in GC.Methods: TDO2 expression was evaluated in public data of The Cancer Genome Atlas cohort STAD and in two different GC cohorts. Correlation between TDO2 and immune cell infiltrates as well as PD-L1 tumor staining was investigated. The biofunction of TDO2 was examined with MTT, colony formation, and spheroid formation assays by RNA interference.Results: TDO2 expression was correlated with both progressive disease and clinical outcome, and its expression was an independent predictor of prognosis in GC. TDO2 expression was correlated with infiltration of immune cells and tumor expression of PD-L1. Inhibition of TDO2 expression suppressed cell proliferation, colony formation, and cell invasion of GC cells. Additionally, suppression of TDO2 expression inhibited spheroid body-formation and viability of GC organoids.Conclusion: Our data show that TDO2 might be a crucial marker for predicting prognosis and targeted therapy in GC.","PeriodicalId":19805,"journal":{"name":"Pathobiology","volume":"90 1","pages":"44-55"},"PeriodicalIF":5.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9180099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Disruptive and Truncating TP53 Mutations Are Associated with African-Ancestry and Worse Prognosis in Brazilian Patients with Lung Adenocarcinoma. 破坏性和截短性TP53突变与巴西肺腺癌患者的非洲祖先和更糟糕的预后有关。

IF 5 4区医学

Pathobiology Pub Date : 2023-01-01 Epub Date: 2023-04-08 DOI: 10.1159/000530587

Rodrigo de Oliveira Cavagna, Icaro Alves Pinto, Flávia Escremim de Paula, Gustavo Noriz Berardinelli, Débora Sant'Anna, Iara Santana, Vinicius Duval da Silva, Eduardo Caetano Albino Da Silva, José Elias Miziara, Josiane Mourão Dias, Augusto Antoniazzi, Alexandre Jacinto, Pedro De Marchi, Miguel Angel Molina-Vila, Leticia Ferro Leal, Rui Manuel Reis

{"title":"Disruptive and Truncating TP53 Mutations Are Associated with African-Ancestry and Worse Prognosis in Brazilian Patients with Lung Adenocarcinoma.","authors":"Rodrigo de Oliveira Cavagna, Icaro Alves Pinto, Flávia Escremim de Paula, Gustavo Noriz Berardinelli, Débora Sant'Anna, Iara Santana, Vinicius Duval da Silva, Eduardo Caetano Albino Da Silva, José Elias Miziara, Josiane Mourão Dias, Augusto Antoniazzi, Alexandre Jacinto, Pedro De Marchi, Miguel Angel Molina-Vila, Leticia Ferro Leal, Rui Manuel Reis","doi":"10.1159/000530587","DOIUrl":"10.1159/000530587","url":null,"abstract":"Introduction: TP53 is the most frequently mutated gene in lung tumors, but its prognostic role in admixed populations, such as Brazilians, remains unclear. In this study, we aimed to evaluate the frequency and clinicopathological impact of TP53 mutations in non-small cell lung cancer (NSCLC) patients in Brazil.Methods: We analyzed 446 NSCLC patients from Barretos Cancer Hospital. TP53 mutational status was evaluated through targeted next-generation sequencing (NGS) and the variants were biologically classified as disruptive/nondisruptive and as truncating/nontruncating. We also assessed genetic ancestry using 46 ancestry-informative markers. Analysis of lung adenocarcinomas from the cBioportal dataset was performed. We further examined associations of TP53 mutations with patients' clinicopathological features.Results: TP53 mutations were detected in 64.3% (n = 287/446) of NSCLC cases, with a prevalence of 60.4% (n = 221/366) in lung adenocarcinomas. TP53 mutations were associated with brain metastasis at diagnosis, tobacco consumption, and higher African ancestry. Disruptive and truncating mutations were associated with a younger age at diagnosis. Additionally, cBioportal dataset revealed that TP53 mutations were associated with younger age and Black skin color. Patients harboring disruptive/truncating TP53 mutations had worse overall survival than nondisruptive/nontruncating and wild-type patients.Conclusion: TP53 mutations are common in Brazilian lung adenocarcinomas, and their biological characterization as disruptive and truncating mutations is associated with African ancestry and shorter overall survival.","PeriodicalId":19805,"journal":{"name":"Pathobiology","volume":" ","pages":"344-355"},"PeriodicalIF":5.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9257625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Tissue Fixation with a Formic Acid-Deprived Formalin Better Preserves DNA Integrity over Time. 组织固定与甲酸剥夺福尔马林更好地保存DNA完整性随着时间的推移。

IF 5 4区医学

Pathobiology Pub Date : 2023-01-01 DOI: 10.1159/000525523

Enrico Berrino, Laura Annaratone, Paolo Detillo, Dora Grassini, Alberto Bragoni, Anna Sapino, Benedetta Bussolati, Giovanni Bussolati, Caterina Marchiò

{"title":"Tissue Fixation with a Formic Acid-Deprived Formalin Better Preserves DNA Integrity over Time.","authors":"Enrico Berrino, Laura Annaratone, Paolo Detillo, Dora Grassini, Alberto Bragoni, Anna Sapino, Benedetta Bussolati, Giovanni Bussolati, Caterina Marchiò","doi":"10.1159/000525523","DOIUrl":"https://doi.org/10.1159/000525523","url":null,"abstract":"Introduction: Optimization of pre-analytic procedures and tissue processing is a basic requirement for reliable and reproducible data to be obtained. Tissue fixation in formalin represents the extensively favored method for surgical tissue specimen processing in diagnostic pathology; however, formalin fixation exerts a blasting effect on DNA and RNA.Methods: A formic acid-deprived formaldehyde solution was prepared by removing acids with an ion-exchange basic resin and the concentrated, acid-deprived formaldehyde (ADF) solution was employed to prepare a 4% ADF solution in 0.1 M phosphate buffer, pH 7.2-7.4. Human (n = 27) and mouse (n = 20) tissues were fixed in parallel and similar conditions in either ADF or neutral buffered formalin (NBF). DNAs and RNAs were extracted, and fragmentation analyses were performed.Results: Besides no significant differences in terms of extraction yield and absorbance ratio, ADF fixation reduced DNA fragmentation, i.e., the largest fragments (>5,000 bp) were significantly more prevalent in the DNAs purified from ADF-fixed tissues (p < 0.001 in both cohorts). Moreover, we observed that DNA preservation is more stable in ADF-fixed tissue compared to NBF-fixed tissues.Conclusion: Although DNA fragmentation in FFPE tissues is a multifactor process, we showed that the removal of formic acid is responsible for a significant improvement in DNA preservation.","PeriodicalId":19805,"journal":{"name":"Pathobiology","volume":"90 3","pages":"155-165"},"PeriodicalIF":5.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9578500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3