利用非接触式抗体混合技术进行多重术中快速免疫组化,以区分肺癌的组织学表型。

IF 3.5 4区 医学 Q3 CELL BIOLOGY
Pathobiology Pub Date : 2024-06-24 DOI:10.1159/000539640
Shoji Kuriyama, Kazuhiro Imai, Hiroshi Nanjo, Yuki Wakamatsu, Shinogu Takashima, Tsubasa Matsuo, Hidenobu Iwai, Ryo Demura, Haruka Suzuki, Yuzu Harata, Sumire Shibano, Akiyuki Wakita, Yusuke Sato, Kyoko Nomura, Yoshihiro Minamiya
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引用次数: 0

摘要

简介:确定早期肺癌的手术策略需要准确的组织学诊断:确定早期肺癌的手术策略需要准确的组织学诊断。免疫组化(IHC)可对组织学类型进行可靠诊断,但与苏木精和伊红染色相比,需要更多时间和更多肿瘤组织切片。我们旨在评估利用交流电(AC)混合技术进行术中肺癌诊断的新型快速多重 IHC 技术的临床有效性:方法:43 名接受肺癌根治性切除术的患者参与了一项回顾性观察研究。我们制备了肺部肿瘤样本的冷冻切片,采用交流电混合法进行快速 IHC 检测,同时实施了针对甲状腺转录因子-1 + 细胞角蛋白 5、去甲斑蝥素 3 + Napsin A 和 p63 + 含三方基序 29 的多重 IHC 检测方案。然后,我们评估了从快速多重 IHC 中得出的术中诊断结果与最终病理结果之间的一致性:结果:使用快速多重 IHC 所做的病理诊断与最终病理结果的吻合率为 93.0%(Cohen's 𝜅 coefficient = 0.860,95% CI 0.727-0.993)。如果仅考虑腺癌和鳞癌,所有病例的诊断结果均一致:我们建议将快速多重 IHC 作为确定肺部肿瘤手术策略的有效工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Multiplex Intraoperative Rapid Immunohistochemistry with Noncontact Antibody Mixing for Distinguishing the Histologic Phenotype of Lung Cancer.

Introduction: Determining a surgical strategy for early-stage lung cancer requires an accurate histologic diagnosis. Immunohistochemistry (IHC) enables reliable diagnosis of histological types but requires more time and more tumor tissue slides than hematoxylin and eosin staining. We aimed to assess the clinical validity of a new rapid multiplex IHC technique utilizing alternating current (AC) mixing for intraoperative lung cancer diagnosis.

Methods: Forty-three patients who underwent radical resection of lung cancers were enrolled in a retrospective observational study. Frozen sections were prepared from lung tumor samples, and rapid IHC employing AC mixing was implemented alongside a multiplex IHC protocol targeting thyroid transcription factor-1 + cytokeratin 5, desmoglein 3 + Napsin A, and p63 + tripartite motif containing 29. We then evaluated the concordance between intraoperative diagnoses derived from rapid multiplex IHC and final pathology.

Results: The concordance rate between the pathological diagnosis made with added rapid multiplex IHC and the final pathology was 93.0% (Cohen's 𝜅 coefficient = 0.860 and 95% CI: 0.727-0.993). When considering only adenocarcinoma and squamous cell carcinoma, the diagnoses were in agreement for all cases.

Conclusions: We suggest rapid multiplex IHC as a promising tool for determining surgical strategies for lung tumors.

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来源期刊
Pathobiology
Pathobiology 医学-病理学
CiteScore
8.50
自引率
0.00%
发文量
47
审稿时长
>12 weeks
期刊介绍: ''Pathobiology'' offers a valuable platform for the publication of high-quality original research into the mechanisms underlying human disease. Aiming to serve as a bridge between basic biomedical research and clinical medicine, the journal welcomes articles from scientific areas such as pathology, oncology, anatomy, virology, internal medicine, surgery, cell and molecular biology, and immunology. Published bimonthly, ''Pathobiology'' features original research papers and reviews on translational research. The journal offers the possibility to publish proceedings of meetings dedicated to one particular topic.
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