Ilias P Nikas, Sojung Lim, Seock-Ah Im, Kyung-Hun Lee, Dae-Won Lee, Hyebin Lee, Han Suk Ryu
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引用次数: 0
Abstract
Introduction: Immunohistochemistry (IHC) for the estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) biomarkers has prognostic and therapeutic value in breast cancer. This study aimed to compare the expression of ER, PR, and HER2 between paired malignant effusions and tissue samples of breast cancer.
Methods: Our electronic archive was searched for all effusions diagnosed as breast carcinomas within a pre-defined period (January 2018-October 2021). Next, their cell blocks (CBs) were subjected to ER, PR, HER2 IHC, or in situ hybridization, in addition to EGFR IHC. The expression of hormone receptors (HRs) and HER2 was subsequently compared between tissue and effusion cytology samples derived from the same patients.
Results: Only 2/76 (2.6%) of the breast cancer patients analyzed showed a malignant effusion at their initial presentation. ER, PR, and HER2 discordance rates between paired malignant effusions and tissue samples obtained at initial diagnosis were 24.3% (17/73), 40.8% (29/71), and 9.1% (6/66), respectively. The HR-/HER2- status was found more often at effusions compared to paired tissue biopsies obtained at initial diagnosis (30/70 vs. 17/70; p < 0.001). In addition, the HR-/HER2- status was significantly associated with an earlier development of a malignant effusion, when found at initial diagnosis (p < 0.001; log-rank test), first recurrence/metastasis (either solid or effusion) (p = 0.012; log-rank test), effusion samples (p = 0.007; log-rank test), and any tumor sample obtained (p = 0.009; log-rank test). Lastly, EGFR overexpression in the HR-/HER2- effusion samples was significantly associated with a shorter post-effusion survival (p = 0.019; log-rank test).
Conclusion: Serous effusion cytology provides high-quality material for ancillary techniques, especially when CBs are prepared, reflecting cancer heterogeneity.
目的：雌激素受体（ER）、孕酮受体（PR）和人表皮生长因子受体2（HER2）生物标志物的免疫组织化学（IHC）在癌症中具有预后和治疗价值，同时有助于分子分型。本研究旨在确定癌症演变不同阶段的亚型不一致及其临床意义，重点研究诊断为恶性肿瘤的渗出细胞学样本。方法：在我们的电子档案中搜索预定义时间内（2018年1月至2021年10月）诊断为乳腺癌的所有积液病例，并对其细胞块（CBs）进行ER、PR和HER2 IHC或原位杂交。此外，从这些患者先前获得的组织标本中提取了关于相同生物标志物的信息。结果：在分析的癌症患者中，只有2/76（2.6%）的患者在初次出现时出现恶性积液。与最初诊断期间接受的配对活检相比，癌症三阴性乳腺癌（TNBC）表型在渗出CBs中发现的频率明显更高（30/70 vs 16/70；P结论：渗出细胞学为辅助技术提供了高质量的材料，尤其是在制备CBs时，反映了癌症的异质性。
期刊介绍：
''Pathobiology'' offers a valuable platform for the publication of high-quality original research into the mechanisms underlying human disease. Aiming to serve as a bridge between basic biomedical research and clinical medicine, the journal welcomes articles from scientific areas such as pathology, oncology, anatomy, virology, internal medicine, surgery, cell and molecular biology, and immunology. Published bimonthly, ''Pathobiology'' features original research papers and reviews on translational research. The journal offers the possibility to publish proceedings of meetings dedicated to one particular topic.
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