Pathobiology最新文献_第5页

Cytological Features of the Emerging Entity Papillary Renal Neoplasm with Reverse Polarity: A Case Report Highlighting the Relevance of Fine-Needle Aspiration in Renal Masses. 反极性肾乳头状肿瘤的细胞学特征：一个强调细针穿刺与肾肿块相关性的病例报告。

IF 2 4区医学

Pathobiology Pub Date : 2025-01-01 Epub Date: 2025-04-15 DOI: 10.1159/000545894

João Lobo, João Castro, Júlia Azevedo, Cláudia Lobo, Ângelo Rodrigues, Isaac Braga, Rui Freitas, Rui Silva-Santos, Ana Peixoto, Rui Henrique, Luís Leça, João Lobo

{"title":"Cytological Features of the Emerging Entity Papillary Renal Neoplasm with Reverse Polarity: A Case Report Highlighting the Relevance of Fine-Needle Aspiration in Renal Masses.","authors":"João Lobo, João Castro, Júlia Azevedo, Cláudia Lobo, Ângelo Rodrigues, Isaac Braga, Rui Freitas, Rui Silva-Santos, Ana Peixoto, Rui Henrique, Luís Leça, João Lobo","doi":"10.1159/000545894","DOIUrl":"10.1159/000545894","url":null,"abstract":"Introduction: Papillary renal neoplasm with reverse polarity (PRNRP) is an emerging entity characterized by distinctive histomorphological and molecular features. Often identified incidentally in imaging studies, its accurate diagnosis is essential considering its favorable prognosis. However, its cytological features need further characterization, since only one single publication with 2 cases diagnosed in fine-needle aspiration biopsies have been reported to date.Case presentation: We report the case of a 70-year-old man recently diagnosed with primary lung adenocarcinoma. Fine-needle aspiration biopsy of a 15-mm nodule in the left kidney revealed cytological features consistent with a papillary neoplasm with eosinophilic features. Immunocytochemistry showed positivity for PAX-8, CK7, and GATA3 and negativity for TTF-1. Next-generation sequencing (NGS) identified a KRAS G12V mutation. Altogether, these findings enabled the diagnosis of PRNRP. The patient underwent conservative management of the renal tumor, which did not recur at 8 months since diagnosis.Conclusion: PRNRP is a recently described tumor which can be managed conservatively. We describe the third case of PRNRP diagnosed on fine-needle aspiration biopsy, which enabled several diagnostic studies, including immunocytochemistry and NGS confirmation. With this case, we highlight the increasing role of fine-needle aspiration biopsy in the clinical management of a subset of patients with small renal masses.","PeriodicalId":19805,"journal":{"name":"Pathobiology","volume":" ","pages":"288-293"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artificial Intelligence Recognition Model Using Liquid-Based Cytology Images to Discriminate Malignancy and Histological Types of Non-Small-Cell Lung Cancer. 利用液基细胞学图像区分非小细胞肺癌恶性程度和组织学类型的人工智能识别模型

IF 3.5 4区医学

Pathobiology Pub Date : 2025-01-01 Epub Date: 2024-08-28 DOI: 10.1159/000541148

Ryota Tanaka, Yukihiro Tsuboshita, Mitsuaki Okodo, Rei Settsu, Kohei Hashimoto, Keisei Tachibana, Kazumasa Tanabe, Koji Kishimoto, Masachika Fujiwara, Junji Shibahara

{"title":"Artificial Intelligence Recognition Model Using Liquid-Based Cytology Images to Discriminate Malignancy and Histological Types of Non-Small-Cell Lung Cancer.","authors":"Ryota Tanaka, Yukihiro Tsuboshita, Mitsuaki Okodo, Rei Settsu, Kohei Hashimoto, Keisei Tachibana, Kazumasa Tanabe, Koji Kishimoto, Masachika Fujiwara, Junji Shibahara","doi":"10.1159/000541148","DOIUrl":"10.1159/000541148","url":null,"abstract":"Introduction: Artificial intelligence image recognition has applications in clinical practice. The purpose of this study was to develop an automated image classification model for lung cancer cytology using a deep learning convolutional neural network (DCNN).Methods: Liquid-based cytology samples from 8 normal parenchymal (N), 22 adenocarcinoma (ADC), and 15 squamous cell carcinoma (SQCC) surgical specimens were prepared, and 45 Papanicolaou-stained slides were scanned using whole-slide imaging. The final dataset of 9,141 patches consisted of 2,737 N, 4,756 ADC, and 1,648 SQCC samples. Densenet-121 was used as the DCNN to classify N versus malignant (ADC+SQCC) and ADC versus SQCC images. AdamW optimizer and 5-fold cross-validation were used in the training.Results: For malignancy prediction, the sensitivity, specificity, and accuracy were 0.97, 0.85, and 0.94, respectively, in the patch-level classification, and 0.92, 0.88, and 0.91, respectively, in the case-level classification. For SQCC prediction, the sensitivity, specificity, and accuracy were 0.86, 0.91, and 0.90, respectively, in the patch-level classification and 0.73, 0.82, and 0.78, respectively, in the case-level classification.Conclusion: The DCNN model performed excellently in predicting malignancy and histological types of lung cancer. This model may be useful for predicting cytopathological diagnosis in clinical situations by reinforcing training.","PeriodicalId":19805,"journal":{"name":"Pathobiology","volume":" ","pages":"52-62"},"PeriodicalIF":3.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142093666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Role of Microtubule-Associated Serine/Threonine Kinase Like as a Good Prognostic Factor in Oral Squamous Cell Carcinoma and Evidences for a Link with Smad7. 微管相关丝氨酸/苏氨酸激酶样（MASTL）在口腔鳞状细胞癌中作为良好预后因素的新作用以及与Smad7相关的证据

IF 2 4区医学

Pathobiology Pub Date : 2025-01-01 Epub Date: 2025-02-26 DOI: 10.1159/000544183

Esperanza Pozo-Agundo, Miguel Álvarez-González, Paloma Lequerica-Fernández, Sergi Herrera I Nogués, Juan Pablo Rodrigo, Tania Rodríguez-Santamarta, Héctor E Torres-Rivas, Saúl Álvarez-Teijeiro, Juana María García-Pedrero, Mónica Álvarez-Fernández, Juan Carlos de Vicente

{"title":"Novel Role of Microtubule-Associated Serine/Threonine Kinase Like as a Good Prognostic Factor in Oral Squamous Cell Carcinoma and Evidences for a Link with Smad7.","authors":"Esperanza Pozo-Agundo, Miguel Álvarez-González, Paloma Lequerica-Fernández, Sergi Herrera I Nogués, Juan Pablo Rodrigo, Tania Rodríguez-Santamarta, Héctor E Torres-Rivas, Saúl Álvarez-Teijeiro, Juana María García-Pedrero, Mónica Álvarez-Fernández, Juan Carlos de Vicente","doi":"10.1159/000544183","DOIUrl":"10.1159/000544183","url":null,"abstract":"Introduction: Upregulated microtubule-associated serine/threonine kinase like (MASTL), a cell cycle kinase required for a progression through mitosis, expression has been associated to poor prognosis. This study aimed to investigate the clinical relevance of MASTL expression in oral squamous cell carcinoma (OSCC) and a possible mechanistic link with epithelial-mesenchymal transition (EMT).Methods: Immunohistochemical analysis of MASTL, E-cadherin, vimentin, and Smad7 was performed in paraffin-embedded tissue specimens from 148 OSCC patients.Results: Nuclear MASTL expression was detected in 115 (77.7%) OSCC specimens. High MASTL expression was significantly associated with the male gender, smoking habit, T stage, early clinical stage, and absence of vascular invasion. MASTL expression was inversely correlated with Smad7, whereas no association was observed with E-cadherin and vimentin. Patients harboring tumors with low MASTL expression exhibited a poorer overall survival. Smad7 expression was significantly related to reduced disease-specific and overall survival. High levels of MASTL expression were associated with better prognosis in T3 patients, N0 cases, and patients treated by surgery combined with radiotherapy and chemotherapy.Conclusion: The present study uncovers MASTL as a good prognostic factor in OSCC and a potential link with EMT via Smad7.","PeriodicalId":19805,"journal":{"name":"Pathobiology","volume":" ","pages":"189-201"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploration of the Tumour Biological Significance of PCLO in Gastric Cancer: Results from a Large Central European Cohort. 探讨PCLO在癌症中的肿瘤生物学意义-来自中欧大型队列的结果。

IF 5 4区医学

Pathobiology Pub Date : 2024-01-01 Epub Date: 2023-11-07 DOI: 10.1159/000534889

Maximilian Bernhardt, Hans-Michael Behrens, Sandra Krüger, Christoph Röcken

{"title":"Exploration of the Tumour Biological Significance of PCLO in Gastric Cancer: Results from a Large Central European Cohort.","authors":"Maximilian Bernhardt, Hans-Michael Behrens, Sandra Krüger, Christoph Röcken","doi":"10.1159/000534889","DOIUrl":"10.1159/000534889","url":null,"abstract":"Introduction: A recent multiregional whole-exome sequencing of 48 tumour samples from 9 gastric adenocarcinomas discovered PCLO mutations in 23 (47.9%) tumour samples. Based on that unexpected high prevalence of PCLO mutations, we hypothesized a tumour biological significance of PCLO in gastric cancer (GC).Methods: Tumour samples (whole tissue sections) obtained from 466 patients resected for therapy-naive GC were stained with an anti-PCLO antibody. The histoscore for tumour cells and the presence of immunostaining of stromal cells and tumour vessels was documented for each case. An algorithm for PCLO immunopositivity was formed and correlated with clinicopathological patient characteristics.Results: 175 GCs were classified as PCLO positive within tumour cells, and 291 as negative. Stromal cells were positive for PCLO in 106 cases and tumour vessels in 84. PCLO-positive GCs more often showed an intestinal phenotype, a lower T category and were more commonly associated with Helicobacter pylori infection. A separate analysis of PCLO expression in intestinal and diffuse type GCs, respectively, showed no significant correlations. Patients with PCLO negative/low tumour cells showed a shortened overall (14.0 ± 1.4 vs. 16.0 ± 1.8 months) and tumour-specific survival (15.0 ± 1.6 months vs. 17.9 ± 3.6). Comparison of PCLOs genotype with its phenotype in 48 tumour samples obtained from nine cases showed no direct correlations with missense mutations.Conclusion: Our data provide evidence that PCLO is differentially expressed in GC and might delay tumour progression.","PeriodicalId":19805,"journal":{"name":"Pathobiology","volume":" ","pages":"187-195"},"PeriodicalIF":5.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11126201/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71484696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expanding the Spectrum of Sarcoma with an Internal Tandem Duplication of BCOR: A Non-Pediatric Nasosinusal Case. 扩大 BCOR 内部串联重复肉瘤的范围：一例非儿童鼻咽癌病例。

IF 3.5 4区医学

Pathobiology Pub Date : 2024-01-01 Epub Date: 2024-05-08 DOI: 10.1159/000539239

Florian Piques, Martin Penicaud, Wassim Essamet, Simon Cabello-Aguilar, Aude Trinquet, Julie A Vendrell, Valérie Costes, Jérôme Solassol

{"title":"Expanding the Spectrum of Sarcoma with an Internal Tandem Duplication of BCOR: A Non-Pediatric Nasosinusal Case.","authors":"Florian Piques, Martin Penicaud, Wassim Essamet, Simon Cabello-Aguilar, Aude Trinquet, Julie A Vendrell, Valérie Costes, Jérôme Solassol","doi":"10.1159/000539239","DOIUrl":"10.1159/000539239","url":null,"abstract":"Introduction: Undifferentiated small round-cell sarcomas with BCL6 corepressor (BCOR) alterations, such as an internal tandem duplication (ITD) within exon 15, are typically described as a pediatric group of Ewing-like small round-cell sarcomas.Case presentation: In contrast to this notion, we report the case of a 71-year-old woman with a nasosinusal sarcoma featuring a BCOR ITD. To the best of our knowledge, this presence had not been previously documented in a sarcoma of the nasal and sinus cavities in an elderly patient. The identified duplication shares a similar minimal critical region as described in clear-cell sarcomas of the kidney in children. This alteration, located within the PCGF1 binding domain, is believed to disrupt the activity of PRC1.1.Conclusion: This case underscores the need for in-depth research into the molecular biology of these rare tumors and explores potential alternative treatment options. The patient achieved remission after two cycles of doxorubicin and cyclophosphamide chemotherapy, highlighting the promise of potential therapeutic options for BCOR ITD sarcomas.","PeriodicalId":19805,"journal":{"name":"Pathobiology","volume":" ","pages":"370-374"},"PeriodicalIF":3.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140892194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ATF4 as a Prognostic Marker and Modulator of Glutamine Metabolism in Oestrogen Receptor-Positive Breast Cancer. ATF4 作为雌激素受体阳性乳腺癌的预后标志和谷氨酰胺代谢调节剂

IF 3.5 4区医学

Pathobiology Pub Date : 2024-01-01 Epub Date: 2024-06-11 DOI: 10.1159/000539564

Roshni Patel, Lutfi H Alfarsi, Rokaya El-Ansari, Brendah K Masisi, Busra Erkan, Ali Fakroun, Ian O Ellis, Emad A Rakha, Andrew R Green

{"title":"ATF4 as a Prognostic Marker and Modulator of Glutamine Metabolism in Oestrogen Receptor-Positive Breast Cancer.","authors":"Roshni Patel, Lutfi H Alfarsi, Rokaya El-Ansari, Brendah K Masisi, Busra Erkan, Ali Fakroun, Ian O Ellis, Emad A Rakha, Andrew R Green","doi":"10.1159/000539564","DOIUrl":"10.1159/000539564","url":null,"abstract":"Introduction: ATF4, a stress-responsive transcription factor that upregulates adaptive genes, is a potential prognostic marker and modulator of glutamine metabolism in breast cancer. However, its exact role remains to be elucidated.Methods: ATF4 expression was evaluated at genomic and transcriptomic levels using METABRIC (n = 1,980), GeneMiner (n = 4,712), and KM-Plotter datasets. Proteomic expression was assessed via immunohistochemistry (n = 2,225) in the Nottingham Primary Breast Cancer Series. ATF4 genomic copy number (CN) variation and mRNA/protein in association with clinicopathological parameters, amino acid transporters (AATs), and patient outcome were investigated.Results: Genomic, transcriptomic, and proteomic overexpression of ATF4 was associated with more aggressive ER-negative tumours. ATF4 mRNA and protein expression were significantly associated with increased expression of glutamine related AATs including SLC1A5 (p < 0.01) and SLC7A11 (p < 0.02). High ATF4 and SLC1A5 protein expression was significantly associated with shorter breast cancer-specific survival (p < 0.01), especially in ER+ tumours (p < 0.01), while high ATF4 and SLC7A11 protein expression was associated with shorter survival (p < 0.01).Conclusion: These findings suggest a complex interplay between ATF4 and AATs in breast cancer biology and underscore the potential role for ATF4 as a prognostic marker in ER+ breast cancer, offering a unique opportunity for risk stratification and personalized treatment strategies.","PeriodicalId":19805,"journal":{"name":"Pathobiology","volume":" ","pages":"411-421"},"PeriodicalIF":3.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11614298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141306502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment and Characterization of Salivary Gland-Specific Injury in Transgenic Mice Model. 建立唾液腺特异性损伤转基因小鼠模型并确定其特征。

IF 3.5 4区医学

Pathobiology Pub Date : 2024-01-01 Epub Date: 2024-07-24 DOI: 10.1159/000539967

Daisuke Omagari, Ryoko Ushikoshi-Nakayama, Tomoe Yamazaki, Hiroko Inoue, Kana Bando, Naoyuki Matsumoto, Ichiro Saito

{"title":"Establishment and Characterization of Salivary Gland-Specific Injury in Transgenic Mice Model.","authors":"Daisuke Omagari, Ryoko Ushikoshi-Nakayama, Tomoe Yamazaki, Hiroko Inoue, Kana Bando, Naoyuki Matsumoto, Ichiro Saito","doi":"10.1159/000539967","DOIUrl":"10.1159/000539967","url":null,"abstract":"Introduction: Many mouse models for autoimmune diseases also have lesions in non-target organs, which may make it difficult to determine whether the target organ lesion is primary or secondary. Hyposalivation has conventionally been studied using genetically modified mouse models for Sjogren's syndrome as well as spontaneous autoimmune mice with systemic lesions, none of which has salivary gland-specific injury.Methods: In this study, we established a salivary gland-specific injury mouse model using the toxin receptor-mediated cell knockout (TRECK) system by gene modification with the transgene composed of the 5' untranslated region of human salivary mucin gene MUC7 (highly expressed specifically in human salivary gland) inserted at the upstream of hHB-EGF (diphtheria toxin receptor) in the TRECK vector.Results: In this transgenic mouse model, we confirmed salivary gland-specific expression of hHB-EGF gene, and hyposalivation after treatment with diphtheria toxin. Histological assessment of the salivary gland from these mice showed granular convoluted tubule epithelial cells destruction at the same position as a positivity in TUNEL assay.Conclusion: This transgenic mouse model may become a useful tool for elucidating the mechanisms involved in hyposalivation and for developing pharmaceuticals and tissue regenerative medical products for this condition.","PeriodicalId":19805,"journal":{"name":"Pathobiology","volume":" ","pages":"434-441"},"PeriodicalIF":3.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Early Events in Serrated Pathway Colorectal Tumorigenesis by Using Digital Spatial Profiling. 利用数字空间图谱鉴定锯齿状通路结直肠肿瘤发生的早期事件。

IF 3.5 4区医学

Pathobiology Pub Date : 2024-01-01 Epub Date: 2024-06-05 DOI: 10.1159/000539612

Min-Cheng Su, Ching-Hsiang Hsu, Ko-Chen Chen, Jun-Ru Lin, Huei-Ying Li, Yi-Ting Fang, Ruby Yun-Ju Huang, Yung-Ming Jeng

{"title":"Identification of Early Events in Serrated Pathway Colorectal Tumorigenesis by Using Digital Spatial Profiling.","authors":"Min-Cheng Su, Ching-Hsiang Hsu, Ko-Chen Chen, Jun-Ru Lin, Huei-Ying Li, Yi-Ting Fang, Ruby Yun-Ju Huang, Yung-Ming Jeng","doi":"10.1159/000539612","DOIUrl":"10.1159/000539612","url":null,"abstract":"Introduction: The colorectal serrated pathway involves precursor lesions known as sessile serrated lesions (SSL) and traditional serrated adenomas (TSA). Mutations in BRAF or KRAS are crucial early events in this pathway. Additional genetic and epigenetic changes contribute to the progression of these lesions into high-grade lesions and, eventually, invasive carcinoma.Methods: We employed digital spatial profiling to investigate the transcriptional changes associated with SSL and TSA. The genes identified are confirmed by immunohistochemical (IHC) staining. Colorectal cancer (CRC) cell lines with CEACAM6 overexpression and knockdown were established to study the roles of CEACAM6 on tumorigenesis of CRC.Results: Ten genes were upregulated in SSL and TSA, and seven were upregulated in both types of lesions. IHC staining confirmed overexpression of CEACAM6, LCN2, KRT19, and lysozyme in SSL and TSA. CEACAM6 expression is an early event in the serrated pathway but a late event in the conventional pathway. Using cell line models, we confirmed that CEACAM6 promotes CRC cells' proliferation, migration, and invasion abilities.Conclusion: These results highlight that the transcriptional changes in the early stages of tumorigenesis exhibit relative uniformity. Identifying these early events may hold significant promise in elucidating the mechanisms behind tumor initiation.","PeriodicalId":19805,"journal":{"name":"Pathobiology","volume":" ","pages":"393-410"},"PeriodicalIF":3.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11614314/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141237299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low-Grade Adenosquamous Carcinoma of the Breast Masquerading as a Fibroepithelial Lesion on Core Biopsy: A Challenging Case. 在核心活检中伪装成纤维上皮病变的低级别乳腺腺鳞癌：一个棘手的病例。

IF 3.5 4区医学

Pathobiology Pub Date : 2024-01-01 Epub Date: 2024-07-17 DOI: 10.1159/000540029

Natthawadee Laokulrath, Esther Chuwa, Mihir Gudi, Puay Hoon Tan

引用次数: 0

CD15 Is a Risk Predictor and a Novel Target in Clear Cell Renal Cell Carcinoma. CD15是透明细胞肾细胞癌的风险预测因子和新靶点。

IF 5 4区医学

Pathobiology Pub Date : 2024-01-01 Epub Date: 2023-11-14 DOI: 10.1159/000535201

Philipp Joachim Stenzel, Mario Schindeldecker, Larissa Seidmann, Esther Herpel, Markus Hohenfellner, Gencay Hatiboglu, Sebastian Foersch, Stefan Porubsky, Stephan Macher-Goeppinger, Wilfried Roth, Katrin Elisabeth Tagscherer

{"title":"CD15 Is a Risk Predictor and a Novel Target in Clear Cell Renal Cell Carcinoma.","authors":"Philipp Joachim Stenzel, Mario Schindeldecker, Larissa Seidmann, Esther Herpel, Markus Hohenfellner, Gencay Hatiboglu, Sebastian Foersch, Stefan Porubsky, Stephan Macher-Goeppinger, Wilfried Roth, Katrin Elisabeth Tagscherer","doi":"10.1159/000535201","DOIUrl":"10.1159/000535201","url":null,"abstract":"Introduction: Tumor cells use adhesion molecules like CD15 or sialylCD15 (sCD15) for metastatic spreading. We analyzed the expression of CD15 and sCD15 in clear cell renal cell carcinoma (ccRCC) regarding prognosis.Methods: A tissue microarray containing tissue specimens of 763 patients with ccRCC was immunohistochemically stained for CD15 and sCD15, their expression quantified using digital image analysis, and the impact on patients' survival analyzed. The cell lines 769p and 786o were stimulated with CD15 or control antibody in vitro and the effects on pathways activating AP-1 and tumor cell migration were examined.Results: ccRCC showed a broad range of CD15 and sCD15 expression. A high CD15 expression was significantly associated with favorable outcome (p < 0.01) and low-grade tumor differentiation (p < 0.001), whereas sCD15 had no significant prognostic value. Tumors with synchronous distant metastasis had a significantly lower CD15 expression compared to tumors without any (p < 0.001) or with metachronous metastasis (p < 0.01). Tumor cell migration was significantly reduced after CD15 stimulation in vitro, but there were no major effects on the activating pathways of AP-1.Conclusion: CD15, but not sCD15, qualifies as a biomarker for risk stratification and as an interesting novel target in ccRCC. Moreover, the data indicate a contribution of CD15 to metachronous metastasis. Further research is warranted to decipher the intracellular pathways of CD15 signaling in ccRCC in order to characterize the CD15 effects on ccRCC more precisely.","PeriodicalId":19805,"journal":{"name":"Pathobiology","volume":" ","pages":"219-229"},"PeriodicalIF":5.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11151972/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"107591957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0