MycologyPub Date : 2022-06-20eCollection Date: 2022-01-01DOI: 10.1080/21501203.2022.2079745
Priyashini Dhaver, Brett Pletschke, Bruce Sithole, Roshini Govinden
{"title":"Isolation, screening, preliminary optimisation and characterisation of thermostable xylanase production under submerged fermentation by fungi in Durban, South Africa.","authors":"Priyashini Dhaver, Brett Pletschke, Bruce Sithole, Roshini Govinden","doi":"10.1080/21501203.2022.2079745","DOIUrl":"https://doi.org/10.1080/21501203.2022.2079745","url":null,"abstract":"<p><p>Fungi are renowned for their ability to produce extracellular enzymes into their surrounding environment. Xylanases are hydrolytic enzymes capable of xylan degradation. The objectives of this study were to isolate, screen for potential xylanolytic fungi from soil and tree bark samples from three locations in South Africa and to determine their growth conditions for maximum xylanase production. Forty-six isolates were obtained based on clearing zone formation on xylan-enriched agar plates using Congo red indicator. Xylanase activity was quantified during submerged fermentation. Isolate MS5, identified as <i>Trichoderma harzianum</i> with the highest enzyme activity (38.17 U/ml) was selected for further studies based on thermophilic properties (70°C) and pH (5.0). The culture conditions; incubation period (5 days), agitation speed (160 rpm) wheat bran (1%) and ammonium sulphate (1.2%) were optimised further. Biochemical characterisation of the crude enzyme revealed two pH and temperature optima (pH 6.0 at 60°C and 70°C, pH 8.0 at 55°C and 75°C). The enzyme retained >70% activity after 4 h at pH 6.0 at 70°C. SDS-PAGE revealed multiple protein bands with a prominent band at 70 kDa. Substrate Native PAGE revealed multiple isoforms between 55 and 130 kDa. This enzyme will be beneficial for applications in the animal feed and biofuels industries.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"13 4","pages":"271-292"},"PeriodicalIF":4.2,"publicationDate":"2022-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673795/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40485811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologyPub Date : 2022-06-20eCollection Date: 2022-01-01DOI: 10.1080/21501203.2022.2089755
Viktor Papp, Yu-Cheng Dai
{"title":"What is the correct scientific name for \"Fuling\" medicinal mushroom?","authors":"Viktor Papp, Yu-Cheng Dai","doi":"10.1080/21501203.2022.2089755","DOIUrl":"https://doi.org/10.1080/21501203.2022.2089755","url":null,"abstract":"<p><p>In recent years, the scientific names of many cultivated and well-known medicinal fungal species have been changed. However, the results of taxonomic and nomenclature works on these economically important fungi are often overlooked or ignored in applied researches. The incorrect use of scientific names may cause uncertainty in research and in the global medicinal mushroom market. In this paper, we briefly review the current taxonomy and nomenclature of \"Fuling\" medicinal mushroom and make a proposal for biochemists, pharmacists and businessmen on the correct use of scientific names related to this species. Based on the recent taxonomic results and nomenclatural proposals, the use of the names <i>Wolfiporia extensa, W. cocos</i> and especially <i>Poria cocos</i> for the \"Fuling\" mushroom are incorrect and misleading; therefore, the acceptance of the names <i>Pachyma hoelen</i> or <i>Wolfiporia hoelen</i> is recommended.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"13 3","pages":"207-211"},"PeriodicalIF":4.2,"publicationDate":"2022-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354628/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40609164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Preparation, structural characterisation, and antioxidant activities of polysaccharides from eight boletes (Boletales) in tropical China.","authors":"Run Tian, Hui Chai, Jun-Qiang Qiu, Zhi-Qun Liang, Hui-Jing Xie, Yong Wang, Nian-Kai Zeng","doi":"10.1080/21501203.2022.2069172","DOIUrl":"10.1080/21501203.2022.2069172","url":null,"abstract":"<p><p>Polysaccharides in boletes (Boletales) are economically significant to both function food and medicinal industries. The polysaccharides were extracted from the fruit bodies of eight boletes, namely, <i>Aureoboletus longicollis, Butyriboletus hainanensis, Crocinoboletus rufoaureus, Hemioporus japonicus, Neoboletus infuscatus, Neoboletus obscureumbrinus, Tylopilus otsuensis, Xanthoconium fusciceps</i>, which were collected from tropical China; their physicochemical properties and antioxidant activities were characterised and evaluated, respectively. The results revealed that the polysaccharides among the eight boletes were mainly composed of glucose, mannose, and galactose, with a broad molecular weight range, and contained a pyranose ring revealed by FT-IR and NMR spectral analyses. Many factors such as different species of boletes, geographic conditions, molecular weight, configuration, and monosaccharide content may affect the antioxidant power of polysaccharides, simultaneously, instead of one single factor. The antioxidant activities of the polysaccharides were measured according to <i>in vitro</i> assays of DPPH scavenging, superoxide anion scavenging, and ferrous ion reducing tests. The polysaccharide of <i>C. rufoaureus</i> has greatly superior antioxidant activity and it could serve as potential functional food or medicine.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"13 3","pages":"195-206"},"PeriodicalIF":4.6,"publicationDate":"2022-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354634/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40609162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Novel indole diketopiperazine stereoisomers from a marine-derived fungus <i>Aspergillus</i> sp.","authors":"Xinyang Li, Jinzhong Xu, Pinmei Wang, Wanjing Ding","doi":"10.1080/21501203.2022.2069173","DOIUrl":"10.1080/21501203.2022.2069173","url":null,"abstract":"<p><p>Four dimeric diketopiperazine stereoisomers (<b>1-4</b>) including two new ones (<b>1-2</b>) had been isolated from the culture broth of one marine-derived fungus <i>Aspergillus</i> sp. Z3, which was found in the gut of a marine isopod <i>Ligia exotica</i>. The planner structures and absolute configurations of the new compounds were determined by combination of NMR, HRESIMS, electronic circular dichroism calculation, Marfey's method as well as single-crystal X-ray diffraction. Their cytotoxicity against the prostate cancer PC3 cell line was assayed by the MTT method.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 1","pages":"1-10"},"PeriodicalIF":4.6,"publicationDate":"2022-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9930829/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9314926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologyPub Date : 2022-04-03DOI: 10.1080/21501203.2022.2058638
Hongyi Shen, D. Bao, D. J. Bhat, H. Su, Zong-Long Luo
{"title":"Lignicolous freshwater fungi in Yunnan Province, China: an overview","authors":"Hongyi Shen, D. Bao, D. J. Bhat, H. Su, Zong-Long Luo","doi":"10.1080/21501203.2022.2058638","DOIUrl":"https://doi.org/10.1080/21501203.2022.2058638","url":null,"abstract":"ABSTRACT Yunnan Province is one of the rich biodiversity hotspots with abundant resources of lignicolous freshwater fungi. A total of 281 species of lignicolous freshwater fungi from 1986 to the present in Yunnan Province. They are mostly distributed in the classes Dothideomycetes and Sordariomycetes, a few species in the Eurotiomycetes and Leotiomycetes, and rarely reported in Orbiliomycetes and Pezizomycetes. Lignicolous freshwater fungi can decompose lignocellulose substrates and release energy and nutrients, and thus playing an important role in freshwater environment. This study briefly reviewed the biodiversity and taxonomic status of lignicolous freshwater fungi in Yunnan, the ecological functions of lignicolous freshwater fungi, factors affecting community distribution, application status, and research difficulties.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"13 1","pages":"119 - 132"},"PeriodicalIF":4.2,"publicationDate":"2022-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46879144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologyPub Date : 2022-03-17eCollection Date: 2022-01-01DOI: 10.1080/21501203.2022.2050825
Shalene da Silva da Silva Santos, Angela Aparecida da da Silva, Julio Cesar Polonio, Andressa Domingos Polli, Ravely Casarotti Orlandelli, João Arthur Dos Santos Dos Santos Oliveira, José Usan Torres Brandão Filho, João Lúcio Azevedo, João Alencar Pamphile
{"title":"Influence of plant growth-promoting endophytes <i>Colletotrichum siamense</i> and <i>Diaporthe masirevici</i> on tomato plants (<i>Lycopersicon esculentum</i> Mill.).","authors":"Shalene da Silva da Silva Santos, Angela Aparecida da da Silva, Julio Cesar Polonio, Andressa Domingos Polli, Ravely Casarotti Orlandelli, João Arthur Dos Santos Dos Santos Oliveira, José Usan Torres Brandão Filho, João Lúcio Azevedo, João Alencar Pamphile","doi":"10.1080/21501203.2022.2050825","DOIUrl":"https://doi.org/10.1080/21501203.2022.2050825","url":null,"abstract":"<p><p>The protective and growth-promoting activities of <i>Colletrotrichum</i> and <i>Diaporthe</i> endophytes on tomato plants (<i>Lycopersicon esculentum</i> Mill.) are underexplored. We screened 40 endophytic fungi associated with Mexican shrimp plant (<i>Justicia brandegeana</i>) using an <i>in vitro</i> dual culture assay for <i>Fusarium oxysporum</i>, one of the most important phytopathogens of tomato plants. The three best antagonists, <i>Colletotrichum siamense</i> (JB224.g1), <i>C. siamense</i> (JB252.g1), and <i>Diaporthe masirevicii</i> (JB270), were identified based on multilocus sequence analysis. They were assessed <i>in vitro</i> for their inhibition of <i>F. oxysporum</i> and phosphate solubilisation capacity, and for the production of indole acetic acid. Greenhouse experiments verified the growth-promoting effects of these endophytes and the suppression of <i>F. oxysporum</i> symptoms in tomato plants. Under greenhouse conditions, the JB252.g1 and JB270 isolates showed positive results for seedling emergence speed. The radicular system depth of plants inoculated with JB270 was greater than that in uninoculated plants (27.21 vs 21.95 cm). The soil plant analysis development chlorophyll metre (SPAD) index showed statistically significant results, especially for the endophyte JB224.g1 (36.99) compared to the control plants (30.90) and plants infected solely with <i>F. oxysporum</i> (33.64).</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"13 4","pages":"257-270"},"PeriodicalIF":4.2,"publicationDate":"2022-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40485812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologyPub Date : 2022-02-22eCollection Date: 2022-01-01DOI: 10.1080/21501203.2022.2036841
Baosong Chen, Jinghan Lin, Ao Xu, Dan Yu, Dorji Phurbu, Huanqin Dai, Yi Li, Hongwei Liu
{"title":"Glyceroglycolipids from the solid culture of <i>Ophiocordyceps sinensis</i> strain LY34 isolated from Tibet of China.","authors":"Baosong Chen, Jinghan Lin, Ao Xu, Dan Yu, Dorji Phurbu, Huanqin Dai, Yi Li, Hongwei Liu","doi":"10.1080/21501203.2022.2036841","DOIUrl":"https://doi.org/10.1080/21501203.2022.2036841","url":null,"abstract":"<p><p><i>Ophiocordyceps sinensis</i> is a well-known entomogenous fungus with its fruiting bodies or cultural mycelium as food and herbal medicines in Asia. While metabolites which could responsible for its potent pharmaceutical effects has long remained to be elucidated. In this work, chemical investigation on the solid culture of <i>O. sinensis</i> strain LY34 led to the discovery of six digalactosyldiacylglycerols (DGDGS, <b>1-6</b>) including one new. The structure of compound <b>1</b> was determined based on the comprehensive spectra analysis, including NMR, MS<sup>n</sup>, IR, and chemical derivatisation. Bioactivity studies showed a weak cytotoxicity of compounds <b>1-6</b> against 293 T cell and medium anti-inflammatory activity of compounds <b>1</b> and <b>2</b> on Raw 264.7 cell. The discovery of DGDGs in <i>O. sinensis</i> provides new insight into the pharmacologically active substances.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"13 3","pages":"185-194"},"PeriodicalIF":4.2,"publicationDate":"2022-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40689134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Optimization for the production of a polyketone <i>3S,4S-</i>DMD from <i>Panus lecomtei</i> (Agaricomycetes) by submerged fermentation.","authors":"Si-Xian Wang, Ping Huang, Hongwei Liu, Yucheng Dai, Xiao-Ling Wang, Gao-Qiang Liu","doi":"10.1080/21501203.2022.2036842","DOIUrl":"https://doi.org/10.1080/21501203.2022.2036842","url":null,"abstract":"<p><p>3,4-Dihydroxy-2,2-dimethyl-chroman derivatives have diverse physiological properties. A polyketone (3<i>S</i>,4<i>S</i>)-3,4-Dihydroxy-6-methoxy-2,2-dimethylchromom (<i>3S,4S-</i>DMD) with antibacterial activity was isolated from the solid culture of rare edible fungus <i>Panus lecomtei</i>. However, the yield of <i>3S,4S-</i>DMD in solid culture of <i>P. lecomtei</i> is very low and the production period are too long. In this work, efficient accumulation of 3<i>S</i>,4<i>S-</i>DMD in <i>P. lecomtei</i> by submerged fermentation is studied. The key fermentation factors of <i>P. lecomtei</i> for <i>3S,4S-</i>DMD production were optimised by single-factor experiment successively, and then a Box-Behnken design (BBD) experiment was carried out to further enhance <i>3S,4S-</i>DMD production. A maximum <i>3S,4S-</i>DMD yield of 196.3 mg/L was obtained at 25.78 g/L glucose, 1.67 g/L MgSO<sub>4</sub> · 7H<sub>2</sub>O, 40°C and 197 r/min, respectively, which increased by 1.3-fold in comparison with that in the non-optimised fermentation conditions. Furthermore, an enhanced yield of <i>3S,4S-</i>DMD (261.6 mg/L) was obtained in 5-L agitated fermenter. The <i>3S,4S</i>-DMD productivity in flask and fermenter reached to 7.26 and 8.07 mg/g per day, respectively, which considerably increased by over 121-fold in comparison with that in the solid fermentation (0.06 mg/g per day). This study presents a potential method for the production of <i>3S,4S</i>-DMD by submerged fermentation.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"13 3","pages":"212-222"},"PeriodicalIF":4.2,"publicationDate":"2022-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40689133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologyPub Date : 2022-02-14eCollection Date: 2022-01-01DOI: 10.1080/21501203.2022.2035005
Bin Lu, Feng-Ming Zhang, Fu-Qiang Yu, Andrea C Rinaldi
{"title":"Ethnobiological notes and volatile profiles of two rare Chinese desert truffles.","authors":"Bin Lu, Feng-Ming Zhang, Fu-Qiang Yu, Andrea C Rinaldi","doi":"10.1080/21501203.2022.2035005","DOIUrl":"https://doi.org/10.1080/21501203.2022.2035005","url":null,"abstract":"<p><p>The production of a distinct profile of volatile organic compounds plays a crucial role in the ecology of hypogeous Ascomycetes, and is also key to their gastronomic relevance. In this study, we explored the aroma components of two rarely investigated Chinese desert truffles, namely <i>Mattirolomyces terfezioides</i> and <i>Choiromyces cerebriformis</i>, using headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). Our investigation revealed the significant presence of sulphur-containing volatiles in the aroma of <i>M. terfezioides</i> but not in <i>C. cerebriformis</i>. We discussed available information on the distribution of these interesting truffles in China and their use as choice food by local people.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"13 3","pages":"177-184"},"PeriodicalIF":4.2,"publicationDate":"2022-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354632/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40609161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Plants and endophytes - a partnership for the coumarin production through the microbial systems.","authors":"Chandrashekar Srinivasa, Govindappa Mellappa, Shashank M Patil, Ramith Ramu, Bhargav Shreevatsa, Chandan Dharmashekar, Shiva Prasad Kollur, Asad Syed, Chandan Shivamallu","doi":"10.1080/21501203.2022.2027537","DOIUrl":"https://doi.org/10.1080/21501203.2022.2027537","url":null,"abstract":"<p><p>Plant-based secondary metabolite production system is well established. However, host-endophyte interaction in the production of secondary metabolite is a new less exploited area that is overcoming barriers and evolving as one of the prospective fields. Endophytes such as bacteria or fungi have the ability to produce some of the secondary metabolites that mimic the plant metabolites therefore escaping the host defence system. Coumarin is one such metabolite with immense biological functions. Most of the studies have demonstrated coumarin production from fungal endophytes but not bacterial endophytes. Herein, we present an overview of all the coumarin derivatives produced from endophytic sources and their biosynthetic pathways. Furthermore, the review also throws light on the isolation of these coumarins and different derivatives with respect to their biological activity. The biotransformation of coumarin derivatives by the action of endophytic fungi is also elaborated. The present review provides an insight on the challenges faced in the coumarin production through fungal endophytes.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"13 4","pages":"243-256"},"PeriodicalIF":4.2,"publicationDate":"2022-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40485815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}