Shalene da Silva da Silva Santos, Angela Aparecida da da Silva, Julio Cesar Polonio, Andressa Domingos Polli, Ravely Casarotti Orlandelli, João Arthur Dos Santos Dos Santos Oliveira, José Usan Torres Brandão Filho, João Lúcio Azevedo, João Alencar Pamphile
{"title":"Influence of plant growth-promoting endophytes <i>Colletotrichum siamense</i> and <i>Diaporthe masirevici</i> on tomato plants (<i>Lycopersicon esculentum</i> Mill.).","authors":"Shalene da Silva da Silva Santos, Angela Aparecida da da Silva, Julio Cesar Polonio, Andressa Domingos Polli, Ravely Casarotti Orlandelli, João Arthur Dos Santos Dos Santos Oliveira, José Usan Torres Brandão Filho, João Lúcio Azevedo, João Alencar Pamphile","doi":"10.1080/21501203.2022.2050825","DOIUrl":null,"url":null,"abstract":"<p><p>The protective and growth-promoting activities of <i>Colletrotrichum</i> and <i>Diaporthe</i> endophytes on tomato plants (<i>Lycopersicon esculentum</i> Mill.) are underexplored. We screened 40 endophytic fungi associated with Mexican shrimp plant (<i>Justicia brandegeana</i>) using an <i>in vitro</i> dual culture assay for <i>Fusarium oxysporum</i>, one of the most important phytopathogens of tomato plants. The three best antagonists, <i>Colletotrichum siamense</i> (JB224.g1), <i>C. siamense</i> (JB252.g1), and <i>Diaporthe masirevicii</i> (JB270), were identified based on multilocus sequence analysis. They were assessed <i>in vitro</i> for their inhibition of <i>F. oxysporum</i> and phosphate solubilisation capacity, and for the production of indole acetic acid. Greenhouse experiments verified the growth-promoting effects of these endophytes and the suppression of <i>F. oxysporum</i> symptoms in tomato plants. Under greenhouse conditions, the JB252.g1 and JB270 isolates showed positive results for seedling emergence speed. The radicular system depth of plants inoculated with JB270 was greater than that in uninoculated plants (27.21 vs 21.95 cm). The soil plant analysis development chlorophyll metre (SPAD) index showed statistically significant results, especially for the endophyte JB224.g1 (36.99) compared to the control plants (30.90) and plants infected solely with <i>F. oxysporum</i> (33.64).</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2022-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673798/pdf/","citationCount":"10","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/21501203.2022.2050825","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"MYCOLOGY","Score":null,"Total":0}
引用次数: 10
Abstract
The protective and growth-promoting activities of Colletrotrichum and Diaporthe endophytes on tomato plants (Lycopersicon esculentum Mill.) are underexplored. We screened 40 endophytic fungi associated with Mexican shrimp plant (Justicia brandegeana) using an in vitro dual culture assay for Fusarium oxysporum, one of the most important phytopathogens of tomato plants. The three best antagonists, Colletotrichum siamense (JB224.g1), C. siamense (JB252.g1), and Diaporthe masirevicii (JB270), were identified based on multilocus sequence analysis. They were assessed in vitro for their inhibition of F. oxysporum and phosphate solubilisation capacity, and for the production of indole acetic acid. Greenhouse experiments verified the growth-promoting effects of these endophytes and the suppression of F. oxysporum symptoms in tomato plants. Under greenhouse conditions, the JB252.g1 and JB270 isolates showed positive results for seedling emergence speed. The radicular system depth of plants inoculated with JB270 was greater than that in uninoculated plants (27.21 vs 21.95 cm). The soil plant analysis development chlorophyll metre (SPAD) index showed statistically significant results, especially for the endophyte JB224.g1 (36.99) compared to the control plants (30.90) and plants infected solely with F. oxysporum (33.64).
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