南非德班地区真菌深层发酵产热稳定木聚糖酶的分离、筛选、初步优化和特性研究。

IF 4.6 2区 生物学 Q1 MYCOLOGY
Mycology Pub Date : 2022-06-20 eCollection Date: 2022-01-01 DOI:10.1080/21501203.2022.2079745
Priyashini Dhaver, Brett Pletschke, Bruce Sithole, Roshini Govinden
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引用次数: 9

摘要

真菌以其在周围环境中产生胞外酶的能力而闻名。木聚糖酶是能够降解木聚糖的水解酶。本研究的目的是从南非三个地点的土壤和树皮样品中分离、筛选潜在的木聚糖分解真菌,并确定其最大木聚糖酶产量的生长条件。利用刚果红指示剂对富木聚糖琼脂板上形成的清除带进行筛选,得到46株分离菌。在深层发酵过程中定量测定木聚糖酶活性。分离物MS5经鉴定为哈兹木霉,酶活性最高(38.17 U/ml),根据其嗜热特性(70°C)和pH(5.0)选择进一步研究。培养条件;进一步优化培养时间(5 d)、搅拌速度(160 rpm)、麦麸(1%)和硫酸铵(1.2%)。粗酶的生化特性显示两个pH和温度最优(60℃和70℃时pH 6.0, 55℃和75℃时pH 8.0)。在pH 6.0、70℃条件下,酶活性维持4 h,活性保持>70%。SDS-PAGE显示在70 kDa处有多条突出的蛋白带。底物Native PAGE在55 ~ 130 kDa范围内显示多个同工异构体。这种酶将有利于动物饲料和生物燃料工业的应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Isolation, screening, preliminary optimisation and characterisation of thermostable xylanase production under submerged fermentation by fungi in Durban, South Africa.

Isolation, screening, preliminary optimisation and characterisation of thermostable xylanase production under submerged fermentation by fungi in Durban, South Africa.

Isolation, screening, preliminary optimisation and characterisation of thermostable xylanase production under submerged fermentation by fungi in Durban, South Africa.

Isolation, screening, preliminary optimisation and characterisation of thermostable xylanase production under submerged fermentation by fungi in Durban, South Africa.

Fungi are renowned for their ability to produce extracellular enzymes into their surrounding environment. Xylanases are hydrolytic enzymes capable of xylan degradation. The objectives of this study were to isolate, screen for potential xylanolytic fungi from soil and tree bark samples from three locations in South Africa and to determine their growth conditions for maximum xylanase production. Forty-six isolates were obtained based on clearing zone formation on xylan-enriched agar plates using Congo red indicator. Xylanase activity was quantified during submerged fermentation. Isolate MS5, identified as Trichoderma harzianum with the highest enzyme activity (38.17 U/ml) was selected for further studies based on thermophilic properties (70°C) and pH (5.0). The culture conditions; incubation period (5 days), agitation speed (160 rpm) wheat bran (1%) and ammonium sulphate (1.2%) were optimised further. Biochemical characterisation of the crude enzyme revealed two pH and temperature optima (pH 6.0 at 60°C and 70°C, pH 8.0 at 55°C and 75°C). The enzyme retained >70% activity after 4 h at pH 6.0 at 70°C. SDS-PAGE revealed multiple protein bands with a prominent band at 70 kDa. Substrate Native PAGE revealed multiple isoforms between 55 and 130 kDa. This enzyme will be beneficial for applications in the animal feed and biofuels industries.

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来源期刊
Mycology
Mycology Medicine-Infectious Diseases
CiteScore
9.10
自引率
0.00%
发文量
18
审稿时长
13 weeks
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