琼脂菌深层发酵生产聚酮3S,4S-DMD的优化研究。

IF 4.6 2区 生物学 Q1 MYCOLOGY
Mycology Pub Date : 2022-02-17 eCollection Date: 2022-01-01 DOI:10.1080/21501203.2022.2036842
Si-Xian Wang, Ping Huang, Hongwei Liu, Yucheng Dai, Xiao-Ling Wang, Gao-Qiang Liu
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引用次数: 1

摘要

3,4-二羟基-2,2-二甲基-铬胺衍生物具有多种生理性质。从稀有食用菌松菇固体培养物中分离到一种具有抗菌活性的聚酮(3S,4S)-3,4-二羟基-6-甲氧基-2,2-二甲基铬(3S,4S- dmd)。然而,在固体培养中,毛草3S、4S-DMD产量很低,生产周期过长。本研究研究了利用深层发酵的方法,高效地积累3S、4S-DMD。通过单因素试验,依次优化了糙皮酵母生产3S、4S-DMD的关键发酵因子,并进行Box-Behnken设计(BBD)试验,进一步提高了3S、4S-DMD的产量。在葡萄糖25.78 g/L、MgSO4·7H2O 1.67 g/L、温度40℃、转速197 r/min条件下,3S、2s - dmd产量最高分别为196.3 mg/L,比未优化的发酵条件提高了1.3倍。此外,在5-L的搅拌发酵罐中,3S,4S-DMD的产量提高了261.6 mg/L。瓶内和发酵罐内3S、4S-DMD产量分别达到7.26和8.07 mg/g / d,较固体发酵(0.06 mg/g / d)显著提高了121倍以上。本研究提出了一种有潜力的深层发酵生产3S、4S-DMD的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Optimization for the production of a polyketone <i>3S,4S-</i>DMD from <i>Panus lecomtei</i> (Agaricomycetes) by submerged fermentation.

Optimization for the production of a polyketone <i>3S,4S-</i>DMD from <i>Panus lecomtei</i> (Agaricomycetes) by submerged fermentation.

Optimization for the production of a polyketone <i>3S,4S-</i>DMD from <i>Panus lecomtei</i> (Agaricomycetes) by submerged fermentation.

Optimization for the production of a polyketone 3S,4S-DMD from Panus lecomtei (Agaricomycetes) by submerged fermentation.

3,4-Dihydroxy-2,2-dimethyl-chroman derivatives have diverse physiological properties. A polyketone (3S,4S)-3,4-Dihydroxy-6-methoxy-2,2-dimethylchromom (3S,4S-DMD) with antibacterial activity was isolated from the solid culture of rare edible fungus Panus lecomtei. However, the yield of 3S,4S-DMD in solid culture of P. lecomtei is very low and the production period are too long. In this work, efficient accumulation of 3S,4S-DMD in P. lecomtei by submerged fermentation is studied. The key fermentation factors of P. lecomtei for 3S,4S-DMD production were optimised by single-factor experiment successively, and then a Box-Behnken design (BBD) experiment was carried out to further enhance 3S,4S-DMD production. A maximum 3S,4S-DMD yield of 196.3 mg/L was obtained at 25.78 g/L glucose, 1.67 g/L MgSO4 · 7H2O, 40°C and 197 r/min, respectively, which increased by 1.3-fold in comparison with that in the non-optimised fermentation conditions. Furthermore, an enhanced yield of 3S,4S-DMD (261.6 mg/L) was obtained in 5-L agitated fermenter. The 3S,4S-DMD productivity in flask and fermenter reached to 7.26 and 8.07 mg/g per day, respectively, which considerably increased by over 121-fold in comparison with that in the solid fermentation (0.06 mg/g per day). This study presents a potential method for the production of 3S,4S-DMD by submerged fermentation.

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来源期刊
Mycology
Mycology Medicine-Infectious Diseases
CiteScore
9.10
自引率
0.00%
发文量
18
审稿时长
13 weeks
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