{"title":"琼脂菌深层发酵生产聚酮3S,4S-DMD的优化研究。","authors":"Si-Xian Wang, Ping Huang, Hongwei Liu, Yucheng Dai, Xiao-Ling Wang, Gao-Qiang Liu","doi":"10.1080/21501203.2022.2036842","DOIUrl":null,"url":null,"abstract":"<p><p>3,4-Dihydroxy-2,2-dimethyl-chroman derivatives have diverse physiological properties. A polyketone (3<i>S</i>,4<i>S</i>)-3,4-Dihydroxy-6-methoxy-2,2-dimethylchromom (<i>3S,4S-</i>DMD) with antibacterial activity was isolated from the solid culture of rare edible fungus <i>Panus lecomtei</i>. However, the yield of <i>3S,4S-</i>DMD in solid culture of <i>P. lecomtei</i> is very low and the production period are too long. In this work, efficient accumulation of 3<i>S</i>,4<i>S-</i>DMD in <i>P. lecomtei</i> by submerged fermentation is studied. The key fermentation factors of <i>P. lecomtei</i> for <i>3S,4S-</i>DMD production were optimised by single-factor experiment successively, and then a Box-Behnken design (BBD) experiment was carried out to further enhance <i>3S,4S-</i>DMD production. A maximum <i>3S,4S-</i>DMD yield of 196.3 mg/L was obtained at 25.78 g/L glucose, 1.67 g/L MgSO<sub>4</sub> · 7H<sub>2</sub>O, 40°C and 197 r/min, respectively, which increased by 1.3-fold in comparison with that in the non-optimised fermentation conditions. Furthermore, an enhanced yield of <i>3S,4S-</i>DMD (261.6 mg/L) was obtained in 5-L agitated fermenter. The <i>3S,4S</i>-DMD productivity in flask and fermenter reached to 7.26 and 8.07 mg/g per day, respectively, which considerably increased by over 121-fold in comparison with that in the solid fermentation (0.06 mg/g per day). This study presents a potential method for the production of <i>3S,4S</i>-DMD by submerged fermentation.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"13 3","pages":"212-222"},"PeriodicalIF":4.6000,"publicationDate":"2022-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354644/pdf/","citationCount":"1","resultStr":"{\"title\":\"Optimization for the production of a polyketone <i>3S,4S-</i>DMD from <i>Panus lecomtei</i> (Agaricomycetes) by submerged fermentation.\",\"authors\":\"Si-Xian Wang, Ping Huang, Hongwei Liu, Yucheng Dai, Xiao-Ling Wang, Gao-Qiang Liu\",\"doi\":\"10.1080/21501203.2022.2036842\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>3,4-Dihydroxy-2,2-dimethyl-chroman derivatives have diverse physiological properties. A polyketone (3<i>S</i>,4<i>S</i>)-3,4-Dihydroxy-6-methoxy-2,2-dimethylchromom (<i>3S,4S-</i>DMD) with antibacterial activity was isolated from the solid culture of rare edible fungus <i>Panus lecomtei</i>. However, the yield of <i>3S,4S-</i>DMD in solid culture of <i>P. lecomtei</i> is very low and the production period are too long. In this work, efficient accumulation of 3<i>S</i>,4<i>S-</i>DMD in <i>P. lecomtei</i> by submerged fermentation is studied. The key fermentation factors of <i>P. lecomtei</i> for <i>3S,4S-</i>DMD production were optimised by single-factor experiment successively, and then a Box-Behnken design (BBD) experiment was carried out to further enhance <i>3S,4S-</i>DMD production. A maximum <i>3S,4S-</i>DMD yield of 196.3 mg/L was obtained at 25.78 g/L glucose, 1.67 g/L MgSO<sub>4</sub> · 7H<sub>2</sub>O, 40°C and 197 r/min, respectively, which increased by 1.3-fold in comparison with that in the non-optimised fermentation conditions. Furthermore, an enhanced yield of <i>3S,4S-</i>DMD (261.6 mg/L) was obtained in 5-L agitated fermenter. The <i>3S,4S</i>-DMD productivity in flask and fermenter reached to 7.26 and 8.07 mg/g per day, respectively, which considerably increased by over 121-fold in comparison with that in the solid fermentation (0.06 mg/g per day). This study presents a potential method for the production of <i>3S,4S</i>-DMD by submerged fermentation.</p>\",\"PeriodicalId\":18833,\"journal\":{\"name\":\"Mycology\",\"volume\":\"13 3\",\"pages\":\"212-222\"},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2022-02-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354644/pdf/\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mycology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/21501203.2022.2036842\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"MYCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/21501203.2022.2036842","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"MYCOLOGY","Score":null,"Total":0}
Optimization for the production of a polyketone 3S,4S-DMD from Panus lecomtei (Agaricomycetes) by submerged fermentation.
3,4-Dihydroxy-2,2-dimethyl-chroman derivatives have diverse physiological properties. A polyketone (3S,4S)-3,4-Dihydroxy-6-methoxy-2,2-dimethylchromom (3S,4S-DMD) with antibacterial activity was isolated from the solid culture of rare edible fungus Panus lecomtei. However, the yield of 3S,4S-DMD in solid culture of P. lecomtei is very low and the production period are too long. In this work, efficient accumulation of 3S,4S-DMD in P. lecomtei by submerged fermentation is studied. The key fermentation factors of P. lecomtei for 3S,4S-DMD production were optimised by single-factor experiment successively, and then a Box-Behnken design (BBD) experiment was carried out to further enhance 3S,4S-DMD production. A maximum 3S,4S-DMD yield of 196.3 mg/L was obtained at 25.78 g/L glucose, 1.67 g/L MgSO4 · 7H2O, 40°C and 197 r/min, respectively, which increased by 1.3-fold in comparison with that in the non-optimised fermentation conditions. Furthermore, an enhanced yield of 3S,4S-DMD (261.6 mg/L) was obtained in 5-L agitated fermenter. The 3S,4S-DMD productivity in flask and fermenter reached to 7.26 and 8.07 mg/g per day, respectively, which considerably increased by over 121-fold in comparison with that in the solid fermentation (0.06 mg/g per day). This study presents a potential method for the production of 3S,4S-DMD by submerged fermentation.