Shalene da Silva da Silva Santos, Angela Aparecida da da Silva, Julio Cesar Polonio, Andressa Domingos Polli, Ravely Casarotti Orlandelli, João Arthur Dos Santos Dos Santos Oliveira, José Usan Torres Brandão Filho, João Lúcio Azevedo, João Alencar Pamphile
{"title":"促进植物生长的内生菌炭疽菌(Colletotrichum siamense)和大孢子菌(Diaporthe masirevici)对番茄植株的影响","authors":"Shalene da Silva da Silva Santos, Angela Aparecida da da Silva, Julio Cesar Polonio, Andressa Domingos Polli, Ravely Casarotti Orlandelli, João Arthur Dos Santos Dos Santos Oliveira, José Usan Torres Brandão Filho, João Lúcio Azevedo, João Alencar Pamphile","doi":"10.1080/21501203.2022.2050825","DOIUrl":null,"url":null,"abstract":"<p><p>The protective and growth-promoting activities of <i>Colletrotrichum</i> and <i>Diaporthe</i> endophytes on tomato plants (<i>Lycopersicon esculentum</i> Mill.) are underexplored. We screened 40 endophytic fungi associated with Mexican shrimp plant (<i>Justicia brandegeana</i>) using an <i>in vitro</i> dual culture assay for <i>Fusarium oxysporum</i>, one of the most important phytopathogens of tomato plants. The three best antagonists, <i>Colletotrichum siamense</i> (JB224.g1), <i>C. siamense</i> (JB252.g1), and <i>Diaporthe masirevicii</i> (JB270), were identified based on multilocus sequence analysis. They were assessed <i>in vitro</i> for their inhibition of <i>F. oxysporum</i> and phosphate solubilisation capacity, and for the production of indole acetic acid. Greenhouse experiments verified the growth-promoting effects of these endophytes and the suppression of <i>F. oxysporum</i> symptoms in tomato plants. Under greenhouse conditions, the JB252.g1 and JB270 isolates showed positive results for seedling emergence speed. The radicular system depth of plants inoculated with JB270 was greater than that in uninoculated plants (27.21 vs 21.95 cm). The soil plant analysis development chlorophyll metre (SPAD) index showed statistically significant results, especially for the endophyte JB224.g1 (36.99) compared to the control plants (30.90) and plants infected solely with <i>F. oxysporum</i> (33.64).</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"13 4","pages":"257-270"},"PeriodicalIF":4.6000,"publicationDate":"2022-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673798/pdf/","citationCount":"10","resultStr":"{\"title\":\"Influence of plant growth-promoting endophytes <i>Colletotrichum siamense</i> and <i>Diaporthe masirevici</i> on tomato plants (<i>Lycopersicon esculentum</i> Mill.).\",\"authors\":\"Shalene da Silva da Silva Santos, Angela Aparecida da da Silva, Julio Cesar Polonio, Andressa Domingos Polli, Ravely Casarotti Orlandelli, João Arthur Dos Santos Dos Santos Oliveira, José Usan Torres Brandão Filho, João Lúcio Azevedo, João Alencar Pamphile\",\"doi\":\"10.1080/21501203.2022.2050825\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The protective and growth-promoting activities of <i>Colletrotrichum</i> and <i>Diaporthe</i> endophytes on tomato plants (<i>Lycopersicon esculentum</i> Mill.) are underexplored. We screened 40 endophytic fungi associated with Mexican shrimp plant (<i>Justicia brandegeana</i>) using an <i>in vitro</i> dual culture assay for <i>Fusarium oxysporum</i>, one of the most important phytopathogens of tomato plants. The three best antagonists, <i>Colletotrichum siamense</i> (JB224.g1), <i>C. siamense</i> (JB252.g1), and <i>Diaporthe masirevicii</i> (JB270), were identified based on multilocus sequence analysis. They were assessed <i>in vitro</i> for their inhibition of <i>F. oxysporum</i> and phosphate solubilisation capacity, and for the production of indole acetic acid. Greenhouse experiments verified the growth-promoting effects of these endophytes and the suppression of <i>F. oxysporum</i> symptoms in tomato plants. Under greenhouse conditions, the JB252.g1 and JB270 isolates showed positive results for seedling emergence speed. The radicular system depth of plants inoculated with JB270 was greater than that in uninoculated plants (27.21 vs 21.95 cm). The soil plant analysis development chlorophyll metre (SPAD) index showed statistically significant results, especially for the endophyte JB224.g1 (36.99) compared to the control plants (30.90) and plants infected solely with <i>F. oxysporum</i> (33.64).</p>\",\"PeriodicalId\":18833,\"journal\":{\"name\":\"Mycology\",\"volume\":\"13 4\",\"pages\":\"257-270\"},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2022-03-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673798/pdf/\",\"citationCount\":\"10\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mycology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/21501203.2022.2050825\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"MYCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/21501203.2022.2050825","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"MYCOLOGY","Score":null,"Total":0}
引用次数: 10
摘要
炭疽菌(Colletrotrichum)和Diaporthe内生菌对番茄(lycopersiicon esculentum Mill.)的保护和促生长作用尚未得到充分的研究。采用番茄植物最重要的病原菌之一尖孢镰刀菌(Fusarium oxysporum)的体外双培养试验，筛选了40种与墨西哥对虾(Justicia brandegeana)相关的内生真菌。通过多位点序列分析，确定了3种最佳拮抗剂，分别是炭疽杆菌(Colletotrichum siamense, JB224.g1)、C. siamense (JB252.g1)和麻氏Diaporthe masirevicii (JB270)。在体外评估了它们对尖孢菌的抑制作用和磷酸盐的增溶能力，以及吲哚乙酸的生产能力。温室试验证实了这些内生菌对番茄植株的促生长作用和对尖孢镰刀菌症状的抑制作用。在温室条件下，JB252。g1和JB270分离株的出苗速度呈阳性。JB270接种植株的根系深度大于未接种植株(27.21 cm vs 21.95 cm)。土壤植物分析发育叶绿素计(SPAD)指数具有显著的统计学意义，尤其是内生植物JB224。g1(36.99)与对照植株(30.90)和单纯感染尖孢镰刀菌植株(33.64)比较，差异有统计学意义。
Influence of plant growth-promoting endophytes Colletotrichum siamense and Diaporthe masirevici on tomato plants (Lycopersicon esculentum Mill.).
The protective and growth-promoting activities of Colletrotrichum and Diaporthe endophytes on tomato plants (Lycopersicon esculentum Mill.) are underexplored. We screened 40 endophytic fungi associated with Mexican shrimp plant (Justicia brandegeana) using an in vitro dual culture assay for Fusarium oxysporum, one of the most important phytopathogens of tomato plants. The three best antagonists, Colletotrichum siamense (JB224.g1), C. siamense (JB252.g1), and Diaporthe masirevicii (JB270), were identified based on multilocus sequence analysis. They were assessed in vitro for their inhibition of F. oxysporum and phosphate solubilisation capacity, and for the production of indole acetic acid. Greenhouse experiments verified the growth-promoting effects of these endophytes and the suppression of F. oxysporum symptoms in tomato plants. Under greenhouse conditions, the JB252.g1 and JB270 isolates showed positive results for seedling emergence speed. The radicular system depth of plants inoculated with JB270 was greater than that in uninoculated plants (27.21 vs 21.95 cm). The soil plant analysis development chlorophyll metre (SPAD) index showed statistically significant results, especially for the endophyte JB224.g1 (36.99) compared to the control plants (30.90) and plants infected solely with F. oxysporum (33.64).
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