MycologyPub Date : 2023-01-01DOI: 10.1080/21501203.2023.2191636
B Van der Merwe, P Herrmann, Karin Jacobs
{"title":"Hericium ophelieae sp. nov., a novel species of Hericium (Basidiomycota: Russulales, Hericiaceae) from the Southern Afrotemperate forests of South Africa.","authors":"B Van der Merwe, P Herrmann, Karin Jacobs","doi":"10.1080/21501203.2023.2191636","DOIUrl":"https://doi.org/10.1080/21501203.2023.2191636","url":null,"abstract":"<p><p>A novel species of <i>Hericium</i> was recently collected in the Afrotemperate forests (Knysna - Amatole region) of Southern Africa. The novel species shares many similar, dentate features common to other species in <i>Hericium</i>, and its basidiome first appears stark white and yellows with age. However, the substrate choice and gloeocystidia and basidiospore sizes of the specimens collected were distinct from other <i>Hericium</i> species. This was confirmed by sequencing the ITS and 28S genetic markers, respectively. The novel species is described as <i>Hericium ophelieae</i> sp. nov. and appears unique as it grows on hardwoods indigenous to Southern Africa. The species has larger basidiospores and wider gloeocystidia compared to its closest relative. <i>H. ophelieae</i> sp. nov. is the first endemic species of the medicinal mushroom genus <i>Hericium</i> to be described from Southern Africa, and the second to be described from Africa, after its closest relative, <i>H. bembedjaense</i>, which was isolated in Cameroon. Although this is the first <i>Hericium</i> to be described from the Southern African region, there are likely others to be discovered, and this study highlights the need for further research into the fungal diversity of Afrotemperate environments.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 2","pages":"133-141"},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10161958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9784065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Species diversity of pathogenic wood-rotting fungi (Agaricomycetes, Basidiomycota) in China.","authors":"Yuan Yuan, Lu-Sen Bian, Ying-Da Wu, Jia-Jia Chen, Fang Wu, Hong-Gao Liu, Guang-Yu Zeng, Yu-Cheng Dai","doi":"10.1080/21501203.2023.2238779","DOIUrl":"https://doi.org/10.1080/21501203.2023.2238779","url":null,"abstract":"<p><p>Wood-rotting basidiomycetes have been investigated in the Chinese forest ecosystem for the past 30 years. Two hundred and five pathogenic wood-decayers belonging to 9 orders, 30 families, and 74 genera have been found in Chinese native forests, plantations, and gardens. Seventy-two species (accounting for 35% of the total pathogenic species) are reported as pathogenic fungi in China for the first time. Among these pathogens, 184 species are polypores, nine are corticioid fungi, eight are agarics and five are hydnoid basidiomycetes. One hundred and seventy-seven species (accounting for 86%) cause white rot, while 28 species (accounting for 14%) result in brown rot; 157 species grow on angiosperm trees (accounting for 76.5%) and 44 species occur on gymnosperm trees (accounting for 21.5%), only four species inhabit both angiosperms and gymnosperms (accounting for 2%); 95 species are distributed in boreal to temperate forests and 110 in subtropical to tropical forests. In addition, 17 species, including <i>Fomitopsis pinicola</i>, <i>Heterobasidion parviporum</i>, and <i>Phellinidium weirii</i> etc. which were previously treated as pathogenic species in China, do not occur in China according to recent studies. In this paper, the host(s), type of forest, rot type, and distribution of each pathogenic species in China are given.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 3","pages":"204-226"},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10424591/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10250971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Four new species of <i>Phyllosticta</i> from China based on morphological and phylogenetic characterization.","authors":"Xiao-Nan Sui, Mei-Jun Guo, Hao Zhou, Cheng-Lin Hou","doi":"10.1080/21501203.2023.2225552","DOIUrl":"https://doi.org/10.1080/21501203.2023.2225552","url":null,"abstract":"<p><p><i>Phyllosticta</i> (<i>Phyllostictaceae, Botryosphaeriales</i>) species are widely distributed globally and constitute a diverse group of pathogenic and endophytic fungi associated with a broad range of plant hosts. In this study, four new species of <i>Phyllosticta</i>, i.e. <i>P. endophytica, P. jiangxiensis, P. machili</i>, and <i>P. xinyuensis</i>, were described using morphological characteristics and multi-locus phylogeny based on the internal transcribed spacer region (ITS) with intervening 5.8S rRNA gene, large subunit of rRNA gene (nrLSU), translation elongation factor 1-alpha gene (<i>tef1</i>), actin gene (<i>act</i>), and glyceraldehyde-3-phosphate dehydrogenase gene (<i>gapdh</i>). <i>Phyllosticta machili</i> is the first species of this genus reported to infect plants of the <i>Machilus</i> genus.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 3","pages":"190-203"},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10424619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10547145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Metabolomics analysis of mycelial exudates provides insights into fungal antagonists of <i>Armillaria</i>.","authors":"Jian Zhan, Jing Yuan, Jianwei Liu, Fengming Zhang, Fuqiang Yu, Yanliang Wang","doi":"10.1080/21501203.2023.2238753","DOIUrl":"https://doi.org/10.1080/21501203.2023.2238753","url":null,"abstract":"<p><p>The genus <i>Armillaria</i> has high edible and medical values, with zones of antagonism often occurring when different species are paired in culture on agar media, while the antagonism-induced metabolic alteration remains unclear. Here, the metabolome of mycelial exudates of two Chinese <i>Armillaria</i> biological species, C and G, co-cultured or cultured separately was analysed to discover the candidate biomarkers and the key metabolic pathways involved in <i>Armillaria</i> antagonists. A total of 2,377 metabolites were identified, mainly organic acids and derivatives, lipids and lipid-like molecules, and organoheterocyclic compounds. There were 248 and 142 differentially expressed metabolites between group C-G and C, C-G, and G, respectively, and fourteen common differentially expressed metabolites including malate, uracil, Leu-Gln-Arg, etc. Metabolic pathways like TCA cycle and pyrimidine metabolism were significantly affected by C-G co-culture. Additionally, 156 new metabolites (largely organic acids and derivatives) including 32 potential antifungal compounds, primarily enriched into biosynthesis of secondary metabolites pathways were identified in C-G co-culture mode. We concluded that malate and uracil could be used as the candidate biomarkers, and TCA cycle and pyrimidine metabolism were the key metabolic pathways involved in <i>Armillaria</i> antagonists. The metabolic changes revealed in this study provide insights into the mechanisms underlying fungal antagonists.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 3","pages":"264-274"},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/02/ff/TMYC_14_2238753.PMC10424624.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10547148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologyPub Date : 2023-01-01DOI: 10.1080/21501203.2022.2096144
Meng Pan, Lu Lin, Chengming Tian, Xinlei Fan
{"title":"Identification and pathogenicity of six fungal species causing canker and dieback disease on golden rain tree in Beijing, China.","authors":"Meng Pan, Lu Lin, Chengming Tian, Xinlei Fan","doi":"10.1080/21501203.2022.2096144","DOIUrl":"https://doi.org/10.1080/21501203.2022.2096144","url":null,"abstract":"<p><p>Golden rain trees (<i>Koelreuteria paniculata</i>) are largely cultivated because of their important ornamental, medicinal, and economic value. However, they are affected by canker and dieback disease to a large extent. To determine the fungi associated with canker and dieback disease of golden rain trees, isolations were obtained from diseased branches and twigs during 2019 and 2020 in greenbelts and nurseries in Beijing, China. Isolates were identified as six species (<i>Allocryptovalsa castaneicola, Botryosphaeria dothidea, Cytospora koelreutericola</i> sp. nov., <i>Dothiorella acericola, Eutypella citricola</i>, and <i>Peroneutypa scoparia</i>) based on morphological features and phylogenetic analyses of ITS, <i>act, rpb2, tef1-α</i>, and <i>tub2</i>. The results of pathogenicity tests indicated that all fungi produced discoloration and <i>Botryosphaeria dothidea</i> was highly aggressive to golden rain tree. In conclusion, this study explored the taxonomy, phylogeny, and pathogenicity of different fungal species associated with canker and dieback disease on golden rain tree and provided fundamental knowledge to improve disease management.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 1","pages":"37-51"},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9930857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9329374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologyPub Date : 2022-12-17eCollection Date: 2023-01-01DOI: 10.1080/21501203.2022.2155261
Nivisti Singh, Bruce Sithole, Roshini Govinden
{"title":"Screening for cellulases and preliminary optimisation of glucose tolerant β-glucosidase production and characterisation.","authors":"Nivisti Singh, Bruce Sithole, Roshini Govinden","doi":"10.1080/21501203.2022.2155261","DOIUrl":"10.1080/21501203.2022.2155261","url":null,"abstract":"<p><p>The search for a novel microbial producer of cellulases including a glucose tolerant β-glucosidase is a challenge as most are inhibited by their product glucose. This study aims to screen for cellulolytic fungi using qualitative and quantitative screening methods. Primary screening revealed 34 of 46 fungal isolates with β-glucosidase activity. Eleven and 13 of these also displayed endoglucanase and exoglucanase activities, respectively. During secondary screening, this number was reduced to 26 β-glucosidase producers with 13 also having endoglucanase and exoglucanase activities. Isolate C1 displayed enhanced production of β-glucosidases in the presence of 0.05 M glucose (69% higher activity). Optimisation of growth conditions for β-glucosidase production by one variable at a time experiments improved production for (isolates) PS1 (64%), MB5 (84%), and C2 (69%). Isolate PS1 identified as <i>Chaetomella</i> sp. BBA70074 displayed the highest tolerance to glucose, retaining 10% of β-glucosidase activity in the presence of 0.8 M glucose. Tolerance to glucose increased to 14% when produced under optimal conditions. β-Glucosidase had a molecular weight of 170 kDa with a pH and temperature optima of 6 and 70°C, respectively. Future studies will include optimisation of the production of the glucose tolerant enzyme by <i>Chaetomella</i> sp. BBA70074.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 2","pages":"91-107"},"PeriodicalIF":4.6,"publicationDate":"2022-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10161942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9485713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologyPub Date : 2022-10-28eCollection Date: 2022-01-01DOI: 10.1080/21501203.2022.2138599
Venkatramanan Mahendrarajan, Vinay Kumar Bari
{"title":"A critical role of farnesol in the modulation of Amphotericin B and Aureobasidin A antifungal drug susceptibility.","authors":"Venkatramanan Mahendrarajan, Vinay Kumar Bari","doi":"10.1080/21501203.2022.2138599","DOIUrl":"https://doi.org/10.1080/21501203.2022.2138599","url":null,"abstract":"<p><p><i>Candida albicans</i> and its related species can cause opportunistic infections such as \"candidiasis\" in immunocompromised individuals with a high morbidity and mortality rate. Several antifungal drugs available in the market are often used to treat infections caused by pathogenic fungi. However, in fungi, the development of resistance against these drugs quickly evolved. <i>Candida</i> is a dimorphic fungus that can switch between yeast to hyphae form, requires an active biosynthesis of membrane constituents. Sphingolipid and ergosterol molecules, are the major fungal plasma membrane components, and their interaction with the antifungal drug can modulate drug susceptibility. A lipophilic compound farnesol acts as a quorum-sensing molecule synthesised by the isoprenoid biosynthesis pathway in the fungal pathogen <i>Candida</i>. Farnesol is secreted in a cell density-dependent manner inhibits hyphae germination and biofilm formation. In this study, we have investigated whether the farnesol molecules affect the drug susceptibility of the antifungal drug Amphotericin B (AmB) which mainly binds with ergosterol, and Aureobasidin A (AbA), a complex sphingolipid biosynthesis inhibitor. Our studies revealed that a non-toxic and low concentration of farnesol can reduce the efficacy of AmB and AbA on yeast cells. This reduction is probably through the alteration in the complex sphingolipid biosynthesis and ATP-binding cassette (ABC) type membrane transport activity. These findings may shed light on a new direction to explore the role of lipid molecules in the antifungal drug resistance mechanisms in pathogenic yeast.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"13 4","pages":"305-317"},"PeriodicalIF":4.2,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40485814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologyPub Date : 2022-07-27eCollection Date: 2022-01-01DOI: 10.1080/21501203.2022.2104950
Paris Chakravarty
{"title":"Mycobiota and mycotoxin-producing fungi in southern California: their colonisation and <i>in vitro</i> interactions.","authors":"Paris Chakravarty","doi":"10.1080/21501203.2022.2104950","DOIUrl":"https://doi.org/10.1080/21501203.2022.2104950","url":null,"abstract":"<p><p>Fungal contamination in water-damaged houses has become a major concern because of their potential health effects. During our survey in 100 water-damaged houses in southern California, we have reported 42 outdoor and 14 indoor fungal species throughout the year. Six commonly occurring indoor fungal species are <i>Alternaria alternata, Aspergillus niger, Chaetomium globosum, Cladosporium herbarum, Penicillium chrysogenum</i> and <i>Stachybotrys chartarum</i>. In the damp building materials, <i>S. chartarum</i> was found to be associated with <i>A. niger, C. globosum</i> and <i>P. chrysogenum</i> but not with <i>A. alternata</i> and <i>C. herbarum. Stachybotrys chartarum</i> showed strong antagonistic effect against <i>A. alternata</i> and <i>C. herbarum</i> and significantly inhibited <i>in vitro</i> growth of <i>A. alternata</i> and <i>C. herbarum</i> but had no effect on <i>A. niger, C. globosum</i> and <i>P. chrysogenum</i>. Two trichothecenes, produced by <i>Stachybotrys</i> sp., trichodermin and trichodermol, significantly inhibited spore germination and <i>in vitro</i> growth of <i>A. alternata</i> and <i>C. herbarum</i> but had no effect on <i>A. niger, C. globosum, P. chrysogenum</i> and <i>S. chartarum</i>. In the damp building materials (drywall, ceiling tile, and oak wood), <i>S. chartarum</i> significantly inhibited the growth of <i>A. alternata</i> and <i>C. herbarum</i> and had no effect on <i>A. niger, C. globosum and P. chrysogenum</i> in these substrata.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"13 4","pages":"293-304"},"PeriodicalIF":4.2,"publicationDate":"2022-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40485810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologyPub Date : 2022-07-05eCollection Date: 2022-01-01DOI: 10.1080/21501203.2022.2094012
Ming Zhang, De-Chun Xie, Chao-Qun Wang, Wang Qiu Deng, Tai-Hui Li
{"title":"New insights into the genus <i>Gyroporus</i> (Gyroporaceae, Boletales), with establishment of four new sections and description of five new species from China.","authors":"Ming Zhang, De-Chun Xie, Chao-Qun Wang, Wang Qiu Deng, Tai-Hui Li","doi":"10.1080/21501203.2022.2094012","DOIUrl":"10.1080/21501203.2022.2094012","url":null,"abstract":"<p><p>Species of <i>Gyroporus</i> from southern China were studied in this study. Based on morphology and molecular phylogenetic analyses of DNA sequences from the nuclear ribosomal internal transcribed spacer (ITS), the nuclear ribosomal large subunit (nrLSU), and the mitochondrial adenosine triphosphate ATP synthase subunit 6 (<i>atp6), Gyroporus</i> was divided into four main branches in the phylogenetic tree, and four sections were firstly proposed i.e. <i>Gyroporus</i> sect. <i>Castaneus, G</i>. sect. <i>Cyanescens, G</i>. sect. <i>Longicystidiatus</i> and <i>G</i>. sect. <i>Pallidus</i>. Five new species, i.e. <i>G. alboluteus, G. atrocyanescens, G. pseudolongicystidiatus, G. pallidus</i> and <i>G. subcaerulescens</i>, were revealed from China, and their phylogenetic positions were also analysed. Among them, <i>G. alboluteus</i> and <i>G. pallidus</i> were nested into the sect. <i>Pallidus</i>, although morphologically similar to <i>G. castaneus; G. atrocyanescens</i> and <i>G. subcaerulescens</i>, with obvious cyanescent oxidation reactions, were nested into the sect. <i>Cyanescens</i>; and <i>G. pseudolongicystidiatus</i> characterised by its long cystidia and was nested into the sect. <i>Longicystidiatus</i>. The new species were formally described and illustrated in the present study, and a key to the sections and species of <i>Gyroporus</i> in China was provided.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"13 3","pages":"223-242"},"PeriodicalIF":4.6,"publicationDate":"2022-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40609163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}