Metabolomics analysis of mycelial exudates provides insights into fungal antagonists of Armillaria.

IF 4.6 2区 生物学 Q1 MYCOLOGY
Jian Zhan, Jing Yuan, Jianwei Liu, Fengming Zhang, Fuqiang Yu, Yanliang Wang
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Abstract

The genus Armillaria has high edible and medical values, with zones of antagonism often occurring when different species are paired in culture on agar media, while the antagonism-induced metabolic alteration remains unclear. Here, the metabolome of mycelial exudates of two Chinese Armillaria biological species, C and G, co-cultured or cultured separately was analysed to discover the candidate biomarkers and the key metabolic pathways involved in Armillaria antagonists. A total of 2,377 metabolites were identified, mainly organic acids and derivatives, lipids and lipid-like molecules, and organoheterocyclic compounds. There were 248 and 142 differentially expressed metabolites between group C-G and C, C-G, and G, respectively, and fourteen common differentially expressed metabolites including malate, uracil, Leu-Gln-Arg, etc. Metabolic pathways like TCA cycle and pyrimidine metabolism were significantly affected by C-G co-culture. Additionally, 156 new metabolites (largely organic acids and derivatives) including 32 potential antifungal compounds, primarily enriched into biosynthesis of secondary metabolites pathways were identified in C-G co-culture mode. We concluded that malate and uracil could be used as the candidate biomarkers, and TCA cycle and pyrimidine metabolism were the key metabolic pathways involved in Armillaria antagonists. The metabolic changes revealed in this study provide insights into the mechanisms underlying fungal antagonists.

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菌丝渗出物的代谢组学分析提供了蜜环菌拮抗真菌的见解。
蜜环菌属具有很高的食用和药用价值,当不同物种在琼脂培养基上配对培养时,经常出现拮抗区,而拮抗诱导的代谢改变尚不清楚。本研究分析了中国两种蜜环菌种C和G共培养或单独培养的菌丝渗出物的代谢组学,以发现蜜环菌拮抗剂的候选生物标志物和关键代谢途径。共鉴定出2377种代谢物,主要是有机酸及其衍生物、脂质和类脂质分子以及有机杂环化合物。C-G组与C组、C-G组和G组差异表达代谢物分别为248种和142种,共有14种差异表达代谢物,包括苹果酸盐、尿嘧啶、Leu-Gln-Arg等。C-G共培养显著影响了TCA循环和嘧啶代谢等代谢途径。此外,在C-G共培养模式下,鉴定出156种新的代谢物(主要是有机酸及其衍生物),包括32种潜在的抗真菌化合物,主要富集到次生代谢物的生物合成途径中。我们认为苹果酸和尿嘧啶可以作为候选生物标志物,TCA循环和嘧啶代谢是蜜环菌拮抗剂的关键代谢途径。本研究揭示的代谢变化为真菌拮抗剂的机制提供了见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Mycology
Mycology Medicine-Infectious Diseases
CiteScore
9.10
自引率
0.00%
发文量
18
审稿时长
13 weeks
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