Microorganisms最新文献

{"title":"<i>Bacillus subtilis</i> B579 Controls Cucumber Fusarium Wilt by Improving Rhizosphere Microbial Community.","authors":"Zongqiang Fan, Jinghan Feng, Lixue Zheng, Yanru Chen, Minglei Wang, Xiangqian Peng, Shuo Wang, Fang Chen","doi":"10.3390/microorganisms13061382","DOIUrl":"10.3390/microorganisms13061382","url":null,"abstract":"<p><p>With continuous improvements in people's environmental awareness, biological control agents have garnered considerable attention owing to their advantageous impacts on improving soil fertility and alleviating plant diseases. <i>Bacillus subtilis</i> (<i>B. subtilis</i>) B579, isolated from the rhizosphere soil of cucumber, has effectively suppressed the growth of pathogenic <i>Fusarium oxysporum</i>. Our study investigates the effects of <i>B. subtilis</i> B579 on the properties of the rhizosphere soil (its physicochemical properties and enzymatic activities) and microbial community of cucumber under <i>Fusarium oxysporum</i> infection. An amplicon sequencing analysis of the microorganisms in the rhizosphere soil was conducted, and the soil's properties were measured. The findings demonstrated that <i>B. subtilis</i> B579 exhibited 73.68% efficacy in controlling cucumber Fusarium wilt disease. B579 pretreatment substantially increased the bacterial and fungi diversity and improved the soil's physicochemical properties (pH level and OC, TN, TP, AK, and AP contents) and enzyme activities, especially those of urease and alkaline phosphatase, which exhibited significant increases of 77.22% and 64.77%, respectively, in comparison to those under the pathogen treatment. Furthermore, the utilization of B579 reduced the abundance of <i>Fusarium</i> while simultaneously increasing the abundance of beneficial groups, including the <i>Bacillus</i>, <i>Paenibacillu</i>s, <i>Sphingomonas</i>, <i>Pseudomonas</i>, <i>Microbacterium</i>, <i>Mortierella</i>, and <i>Trichoderma</i> genera. The RDA showed that the abundance of <i>Bacillus</i>, <i>Paenibacillus</i>, <i>Sphingomonas</i>, and <i>Mortierella</i> in the rhizosphere showed positive correlations with most of the soil properties, whereas <i>Fusarium</i> abundance was negatively correlated with most of the soil's properties. This study provides novel insights into the disease suppression mechanisms of <i>Bacillus subtilis</i> B579, laying the theoretical foundation for its development as a biocontrol agent.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12196314/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fertilization Alters Indicator Species Serving as Bioindicators for Evaluating Agricultural Practices Related to Maize Grain Yield.","authors":"Guoqiang Li, Jiaqing Liu, Wenya Zhang, Jvshui Hu, Peng Shi, Gehong Wei","doi":"10.3390/microorganisms13061384","DOIUrl":"10.3390/microorganisms13061384","url":null,"abstract":"<p><p>Diversified agricultural practices reconfigure agroecosystem services by modifying fertilization, tillage intensities, and cropping patterns, altering soil properties and microbial assemblages. However, microbial communities, as critical bioindicators of soil health and productivity, respond to agricultural disturbances, and the effects of multiple practices on productivity-associated indicator species require further validation. Using 16S and ITS amplicon sequencing, this study employed a field experiment to investigate the effects of agricultural practices on soil properties, maize productivity, and microbial communities under two fertilization treatments. Within each treatment, we assessed correlations between indicator species associated with cropping-tillage practices and soil productivity. Results showed that fertilization significantly altered soil properties, increased maize grain yield by 23.9%, and reshaped bacterial and fungal community structures, increasing bacterial richness by 23% but reducing fungal richness and Shannon index by 15% and 20%, respectively. Furthermore, cropping-tillage practices significantly affected microbial communities and grain yields in both fertilized and unfertilized treatments despite a slight influence on soil properties. Distinct sets of bacterial and fungal indicator species were identified for each fertilization treatment: unfertilized soils harbored 21 dominant bacterial indicator species (e.g., <i>Bacillus</i>, <i>Rhizobium</i>, <i>Streptomyces</i>) and 8 fungal indicators (e.g., <i>Cryptococcus</i>, <i>Gibberella</i>, <i>Tetracladium</i>); fertilized soils contained 24 dominant bacterial indicators (e.g., <i>Fusobacterium</i>, <i>Clostridium</i>, <i>Lactobacillus</i>) and 6 fungal indicators (e.g., <i>Gibberella</i>, <i>Cladosporium</i>, <i>Mortierella</i>). Notably, abundances of specific indicator genera (e.g., bacteria: <i>Bacteroides</i>, <i>Gemmatirosa</i>, <i>Iamia</i>, <i>Lysobacter</i>, <i>Prevotella</i>, <i>Staphylococcus</i>, <i>Sutterella</i>; fungi: <i>Glomus</i>, <i>Fusicolla</i> in unfertilized soil; bacteria: <i>Dinghuibacter</i>, <i>Haliangium</i>, <i>Kribbella</i>, <i>Rhodomicrobium</i>, <i>Terrimonas</i>; fungi: <i>Pulvinula</i> in fertilized soil) correlated positively with grain yields. These findings demonstrate that fertilization reshapes the composition of microbial indicator species significantly associated with maize productivity. Tailored microbial indicator assemblages specific to distinct fertilization strategies are therefore essential for evaluating crop productivity and assessing agricultural practice impacts. Consequently, monitoring these indicator species enables rapid assessment of soil fertility changes, offering guidance for fertilization management.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195582/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Halotolerance of Phytoplankton and Invasion Success of Nostocalean Cyanobacteria Under Freshwater Salinization.","authors":"Izabelė Šuikaitė, Gabrielė Šiurkutė, Robert Ptacnik, Judita Koreivienė","doi":"10.3390/microorganisms13061378","DOIUrl":"10.3390/microorganisms13061378","url":null,"abstract":"<p><p>Disturbed ecosystems are particularly susceptible to biological invasions. Increasing freshwater salinization, caused by anthropogenic factors, can alter the phytoplankton community and favour newly arrived halotolerant species. This study investigates the halotolerance of four Nostocalean cyanobacterial species-the native to Europe, <i>Aphanizomenon gracile</i>, and alien <i>Chrysosporum bergii</i>, <i>Cuspidothrix issatschenkoi</i>, and <i>Sphaerospermopsis aphanizomenoides</i>-using monoculture experiments under varying NaCl concentrations. Additionally, we performed two microcosm experiments to explore shifts in biodiversity in freshwater phytoplankton communities sourced from artificial reservoirs and assess their susceptibility to cyanobacterial invasion under salinity stress. Results showed that all Nostocalean cyanobacteria were halotolerant under mild salinities (up to 1 g/L NaCl), with <i>Chrysosporum bergii</i> and <i>Sphaerospermopsis aphanizomenoides</i> demonstrating the most salt tolerance. In the microcosm experiment, changes in community composition were driven by the halotolerance of dominant groups. Water body 1, dominated by Bacillariophytina, reduced its biomass of phytoplankton at high salinity (5 g/L NaCl), while water body 2, dominated by Chlorophytina, remained stable regardless of disturbance. Both cyanobacteria successfully invaded both halotolerant and halosensitive communities, increasing their dominance as salinity rose. Our findings suggest that anthropogenic stressors such as freshwater salinization can alter the phytoplankton community and increase a competitive advantage to certain taxa, including widespread alien cyanobacteria, potentially promoting invasions and bloom formation.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195237/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Healthcare-Associated <i>Clostridioides difficile</i> Infection: A Hospital-Based Retrospective Study in North Eastern Romania.","authors":"Lidia Oana Stămăteanu, Ionela Larisa Miftode, Claudia Elena Pleşca, Mihnea Eudoxiu Hurmuzache, Doina Carmen Manciuc, Daniela Leca, Egidia Gabriela Miftode","doi":"10.3390/microorganisms13061377","DOIUrl":"10.3390/microorganisms13061377","url":null,"abstract":"<p><p><i>Clostridioides difficile</i> infection (CDI), the most common cause of nosocomial diarrhea, presents with a wide spectrum of clinical manifestations, ranging from mild diarrhea to severe, life-threatening conditions such as pseudomembranous colitis and toxic megacolon. In recent years, both the incidence and severity of CDI have increased, leading to a significant burden in terms of morbidity, mortality, and healthcare costs. We conducted a single-center, retrospective cohort study for 30 months at \"Sf. Parascheva\" Infectious Diseases Clinical Hospital Iași, in North Eastern Romania, aiming to assess the clinical and laboratory characteristics of CDI, as well as treatment approaches and their association with patient outcomes. A total of 534 patients were included during the study period, of whom 484 had favorable outcomes, while 50 have died of the disease. Fever (<i>p</i> = 0.007) and age over 65 (<i>p</i> = 0.001) were associated with prolonged hospitalization. Patients positive for both A and B toxins and GDH had the highest risk of recurrence (<i>p</i> = 0.020). Among comorbidities, obesity was the only condition significantly linked to recurrence (<i>p</i> = 0.001). In female patients over 65 years old, the probability of survival drops below 60% after 21 days of hospitalization, highlighting a critical risk factor in this population. These results underscore the importance of comprehensive risk assessment in CDI, particularly focusing on advanced age and comorbidities, to guide early therapeutic interventions, optimize patient management, and improve clinical outcomes among high-risk populations.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12196210/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterologous Expression and Antimicrobial Mechanism of a Cysteine-Rich Peptide from Barnacle <i>Pollicipes pollicipes</i>.","authors":"Zhicheng He, Zixun Fei, Huishao Shi, Meichuan Huang, Liumi Wei, Junjian Wang, Peng He, Wei Zhang","doi":"10.3390/microorganisms13061381","DOIUrl":"10.3390/microorganisms13061381","url":null,"abstract":"<p><p>The escalating crisis of antimicrobial resistance in aquaculture, driven by the indiscriminate use of antibiotics, underscores the urgent need to develop novel anti-infective agents. This study addresses this requirement by investigating cysteine-rich antimicrobial peptides (AMPs) in understudied crustacean species. A cysteine-rich AMP, designated <i>Pp</i>Rcys1, was identified and characterized from the genome of <i>Pollicipes pollicipes</i>. <i>Pp</i>Rcys1 comprises 104 amino acids, with 85 residues forming the mature peptide region, and exhibits random coils, a CSαβ-fold, and one β-sheet. Our findings demonstrated that recombinant <i>Pp</i>Rcys1 (r<i>Pp</i>Rcys1) possesses broad-spectrum antimicrobial activity against three Gram-positive bacteria (<i>Staphylococcus aureus</i>, <i>Bacillus</i> sp. T2, and <i>Streptococcus agalactiae</i>) and four Gram-negative bacteria (<i>Aeromonas hydrophila</i>, <i>Escherichia coli</i>, <i>Vibrio alginolyticus</i>, and <i>Acinetobacter</i> sp. L3), with minimum inhibitory concentrations ranging from 8 to 32 μM. It exerts antimicrobial effects by inducing membrane disruption without impacting bacterial protease activity, DNA migration, or respiratory chain reductase activity. Further investigation is warranted to determine whether it can target and interfere with intracellular bacterial processes. Our discovery and characterization of this novel AMP provide a promising foundation for its development as an alternative to antibiotics.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195476/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological, Clinical and Analytical Features in Lyme Borreliosis Patients Seropositive for <i>Babesia divergens/venatorum</i>.","authors":"María Folgueras, Luis Miguel González, Aitor Gil, Julio Collazos, Mercedes Rodríguez-Pérez, Laura Pérez-Is, Javier Díaz-Arias, María Meana, Belén Revuelta, Jeremy Gray, Estrella Montero, Víctor Asensi","doi":"10.3390/microorganisms13061383","DOIUrl":"10.3390/microorganisms13061383","url":null,"abstract":"<p><p>Lyme borreliosis (LB), caused by <i>Borrelia burgdorferi</i> sensu lato (s.l.) and babesiosis, caused by <i>Babesia divergens</i> and <i>Babesia venatorum</i>, are both transmitted by the ixodid tick <i>Ixodes ricinus</i>. Although these diseases coexist in Spain and other European regions, no studies have been carried out to assess the impact on the health of patients exposed to both causative pathogens. This retrospective study, conducted in Asturias in northwestern Spain between 2015 and 2017, analyzed the possible complications arising from exposure to these pathogens. To this end, the epidemiological, clinical and analytical features of two groups of a cohort of 120 LB patients exposed to one or two of the pathogens were compared. The cohort comprised 73 patients who had only been infected with <i>Bo. burgdorferi</i> s.l. (Bb group) and 47 patients who were seropositive for <i>Ba. divergens</i>/<i>venatorum</i> in addition to being infected with <i>Bo. burgdorferi</i> s.l. (BbBdv group). The results showed that LB patients who had also been exposed to infection with <i>Babesia</i> spp. (BbBdv group) had significantly more cardiorespiratory symptoms, in particular dyspnea and first- and second-degree atrioventricular (AV) blocks, compared to those who had been infected with <i>Bo. burgdorferi</i> s.l. alone (Bb group). No relevant differences in other symptoms, epidemiological factors or analytical tests were observed between the two groups.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exercise Remodels Akkermansia-Associated Eicosanoid Metabolism to Alleviate Intestinal Senescence: Multi-Omics Insights.","authors":"Chunxia Yu, Xuanyu Liu, Yitong Li, Silin Li, Yating Huang, Sujuan Liu, Heng Shao, Yanna Shen, Li Fu","doi":"10.3390/microorganisms13061379","DOIUrl":"10.3390/microorganisms13061379","url":null,"abstract":"<p><p>Aerobic exercise mitigates age-related intestinal senescence through gut microbiota modulation, but the underlying mechanism has remained unclear. In this study, we performed 16S rRNA sequencing of gut contents from young, old, and old exercise C57BL/6J mice to assess exercise-induced alterations in microbiota community structure. Differential taxa analyses were applied to reveal age-associated bacterial signatures, gut barrier integrity, and systemic inflammation. Additionally, untargeted metabolomic profiling was employed to characterize gut metabolic profiles and reveal the key pathways through differential metabolite enrichment analyses. Aging significantly exacerbated the senescence-associated secretory phenotypes and the overgrowth of pathogenic bacteria in mice. However, aerobic exercise ameliorated these age-related deteriorations, restored gut microbial homeostasis, and reduced intestinal permeability. Notably, exercise intervention led to a significant increase in Akkermansia abundance in feces, establishing this mucin-degrading bacterium as a prominent exercise-responsive microbe. Metabolomic profiling identified eicosanoid metabolism as the most significantly perturbed pathway, and chronic exercise was found to regulate 14,15-Dhet levels. Our multi-omics integration confirmed that exercise is a potent modulator of the gut-microbiota-metabolite axis during aging. Elucidating the \"Akkermansia-eicosanoid signaling\" axis provided mechanistic insights into how exercise promotes healthy aging, identifying novel targets for anti-aging strategies via microbiota.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195349/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional Characterization, Genome Assembly, and Annotation of <i>Geobacillus</i> sp. G4 Isolated from a Geothermal Field in Tacna, Peru.","authors":"Alonso R Poma Ticona, Karita C R Santos, Heber E Ramirez-Arua, Roberto Castellanos, Jéssica Pinheiro Silva, Pedro R Vieira Hamann, Eliane F Noronha, Fabyano A C Lopes","doi":"10.3390/microorganisms13061374","DOIUrl":"10.3390/microorganisms13061374","url":null,"abstract":"<p><p>The genome of <i>Geobacillus</i> sp. G4, a thermophilic bacterium isolated from a geothermal field in Peru, was sequenced and analyzed to evaluate its taxonomic and biotechnological potential. This strain exhibits optimal growth at temperatures between 50 and 70 °C and at a pH range of 6.0-7.5. Phenotypic assays demonstrated extracellular enzymatic activities, including amylases, cellulases, pectinases, and xylanases, highlighting its potential for efficient polysaccharide degradation. The assembled genome comprises approximately 3.4 Mb with a G+C content of 52.59%, containing 3,490 genes, including coding sequences, rRNAs, and tRNAs. Functional annotation revealed genes associated with key metabolic pathways such as glycogen and trehalose biosynthesis, indicating adaptation to carbohydrate-rich environments. Phylogenetic analyses based on ANI and dDDH values identified <i>Geobacillus thermoleovorans</i> KCTC3570 as its closest relative, suggesting a strong evolutionary relationship. Additionally, the genome harbors gene clusters for secondary metabolites such as betalactone and fengycin, suggesting potential industrial and pharmaceutical applications, including bioremediation. The identification of antibiotic resistance genes, specifically those conferring glycopeptide resistance, underscores their relevance for antimicrobial resistance studies. The presence of enzymes like amylases and pullulanase further emphasizes its biotechnological potential, particularly in starch hydrolysis and biofuel production. Overall, this research highlights the significant potential of <i>Geobacillus</i> species as valuable sources of thermostable enzymes and biosynthetic pathways for industrial applications.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12196064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial Metabolic Activity of High-Mountain Lakes in a Context of Increasing Regional Temperature.","authors":"Boyanka Angelova, Ivan Traykov, Silvena Boteva, Martin Tsvetkov, Anelia Kenarova","doi":"10.3390/microorganisms13061375","DOIUrl":"10.3390/microorganisms13061375","url":null,"abstract":"<p><p>Global warming poses a significant threat to lake ecosystems, with high-mountain lakes being among the earliest and most severely impacted. However, the processes affecting water ecology under climate change remain poorly understood. This study investigates, for the first time, the effects of regional warming on three high-mountain lakes, Sulzata, Okoto and Bubreka, located in the Rila Mountains, Bulgaria, by examining shifts in bacterial metabolic capacity in relation to the rate and range of utilizable carbon sources using the Biolog EcoPlate™ assay. Over the last decade, ice-free water temperatures in the lakes have risen by an average of 2.6 °C, leading to increased nutrient concentrations and enhanced primary productivity, particularly in the shallowest lake. Bacterial communities responded to these changes by increasing their metabolic rates and shifting substrate preferences from carbohydrates to carboxylic acids. While the utilization rates of some carbon sources remained stable, others showed significant changes-some increased (e.g., D-galactonic acid γ-lactone and itaconic acid), while others decreased (e.g., α-D-lactose and D-xylose). The most pronounced effects of warming were observed in June, coinciding with the onset of the growing season. These findings suggest that rising temperatures may substantially alter bacterial metabolic potential, contributing to a long-term positive feedback loop between lake nutrient cycling and climate change.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12196075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Emerging Bacterial Leaf Disease in Rice Caused by <i>Pantoea ananatis</i> and <i>Pantoea eucalypti</i> in Northeast China.","authors":"Guohua Duan, Xin Liu, Shaoqi Zhang, Mengzhu Chai, Zhao Peng, Zihan Lin, Dayong Li, Wenxian Sun","doi":"10.3390/microorganisms13061376","DOIUrl":"10.3390/microorganisms13061376","url":null,"abstract":"<p><p>Rice production faces new challenges from emerging diseases due to intensive cultivation practices and climate warming in China. A new rice leaf bacterial disease has recently occurred in Northeast China. The symptoms of the disease are similar to those of bacterial leaf blight. Disease lesions spread along leaf edges and are later dried up due to water loss. In this study, 17 bacterial isolates were identified as the causal agents of the new disease following Koch's postulates. These strains are categorized into two groups based on colony morphology and molecular characterization. Phylogenetic analysis using the five housekeeping genes <i>leuS</i>, <i>gyrB</i>, <i>fusA</i>, <i>pyrG</i>, and <i>rplB</i> reveals that the two groups of the isolates belong to <i>Pantoea ananatis</i> and <i>P. eucalypti</i>, respectively. The new rice disease is caused by <i>P. ananatis</i>, <i>P. eucalypti</i>, or a combination of both bacterial species. A complete genome map has also been assembled for <i>P. eucalypti</i>. Meanwhile, some important virulence factors have been predicted based on gene annotation and determination of extracellular enzymes. Collectively, this study represents the first report of a new rice leaf disease caused by <i>P. eucalypti</i> and the first high-quality genome assembly of <i>P. eucalypti</i> that infects rice leaves.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}