Débora Coraça-Huber, Martina Humez, Klaus-Dieter Kühn
{"title":"Palacos和Simplex丙烯酸水泥中庆大霉素和妥布霉素缓释及抗菌效果的比较研究。","authors":"Débora Coraça-Huber, Martina Humez, Klaus-Dieter Kühn","doi":"10.3390/microorganisms13092174","DOIUrl":null,"url":null,"abstract":"<p><p>Antibiotic-loaded bone cements (ALBCs) are used to prevent and treat periprosthetic joint infections (PJI). This study compares the in vitro release and antibacterial effectiveness of gentamicin from Palacos<sup>®</sup> R+G and tobramycin from Simplex<sup>®</sup> T. Standardized cylindrical specimens of Palacos<sup>®</sup> R+G and Simplex<sup>®</sup> T were incubated in phosphate-buffered saline. Antibiotic release was quantified using high-performance liquid chromatography (HPLC) over 14 and 42 days. Antibacterial efficacy was assessed using inhibition zone tests (IZT) against <i>Staphylococcus aureus</i>, <i>Staphylococcus epidermidis</i>, and <i>Escherichia coli</i> over 42 days. Palacos<sup>®</sup> R+G exhibited a significantly higher and more sustained antibiotic release of gentamicin compared to tobramycin from Simplex<sup>®</sup> T. The cumulative release of gentamicin from Palacos<sup>®</sup> R+G was 1.148 µg/cm<sup>2</sup>, while Simplex<sup>®</sup> T released 198.87 µg/cm<sup>2</sup> tobramycin over 14 days. Inhibition zone tests showed that Palacos<sup>®</sup> R+G maintained antibacterial activity for 42 days, while Simplex<sup>®</sup> T's activity diminished after 14 days. Statistical analysis confirmed significant differences in antibacterial efficacy between the two cements. Palacos<sup>®</sup> R+G demonstrated superior gentamicin release and sustained antibacterial activity compared to tobramycin from Simplex<sup>®</sup> T. These findings suggest that Palacos<sup>®</sup> R+G may offer better clinical outcomes in preventing and treating PJIs.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2000,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12473054/pdf/","citationCount":"0","resultStr":"{\"title\":\"A Comparative Study of Extended Gentamicin and Tobramycin Release and Antibacterial Efficacy from Palacos and Simplex Acrylic Cements.\",\"authors\":\"Débora Coraça-Huber, Martina Humez, Klaus-Dieter Kühn\",\"doi\":\"10.3390/microorganisms13092174\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Antibiotic-loaded bone cements (ALBCs) are used to prevent and treat periprosthetic joint infections (PJI). This study compares the in vitro release and antibacterial effectiveness of gentamicin from Palacos<sup>®</sup> R+G and tobramycin from Simplex<sup>®</sup> T. Standardized cylindrical specimens of Palacos<sup>®</sup> R+G and Simplex<sup>®</sup> T were incubated in phosphate-buffered saline. Antibiotic release was quantified using high-performance liquid chromatography (HPLC) over 14 and 42 days. Antibacterial efficacy was assessed using inhibition zone tests (IZT) against <i>Staphylococcus aureus</i>, <i>Staphylococcus epidermidis</i>, and <i>Escherichia coli</i> over 42 days. Palacos<sup>®</sup> R+G exhibited a significantly higher and more sustained antibiotic release of gentamicin compared to tobramycin from Simplex<sup>®</sup> T. The cumulative release of gentamicin from Palacos<sup>®</sup> R+G was 1.148 µg/cm<sup>2</sup>, while Simplex<sup>®</sup> T released 198.87 µg/cm<sup>2</sup> tobramycin over 14 days. Inhibition zone tests showed that Palacos<sup>®</sup> R+G maintained antibacterial activity for 42 days, while Simplex<sup>®</sup> T's activity diminished after 14 days. Statistical analysis confirmed significant differences in antibacterial efficacy between the two cements. Palacos<sup>®</sup> R+G demonstrated superior gentamicin release and sustained antibacterial activity compared to tobramycin from Simplex<sup>®</sup> T. These findings suggest that Palacos<sup>®</sup> R+G may offer better clinical outcomes in preventing and treating PJIs.</p>\",\"PeriodicalId\":18667,\"journal\":{\"name\":\"Microorganisms\",\"volume\":\"13 9\",\"pages\":\"\"},\"PeriodicalIF\":4.2000,\"publicationDate\":\"2025-09-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12473054/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microorganisms\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.3390/microorganisms13092174\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microorganisms","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/microorganisms13092174","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
A Comparative Study of Extended Gentamicin and Tobramycin Release and Antibacterial Efficacy from Palacos and Simplex Acrylic Cements.
Antibiotic-loaded bone cements (ALBCs) are used to prevent and treat periprosthetic joint infections (PJI). This study compares the in vitro release and antibacterial effectiveness of gentamicin from Palacos® R+G and tobramycin from Simplex® T. Standardized cylindrical specimens of Palacos® R+G and Simplex® T were incubated in phosphate-buffered saline. Antibiotic release was quantified using high-performance liquid chromatography (HPLC) over 14 and 42 days. Antibacterial efficacy was assessed using inhibition zone tests (IZT) against Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli over 42 days. Palacos® R+G exhibited a significantly higher and more sustained antibiotic release of gentamicin compared to tobramycin from Simplex® T. The cumulative release of gentamicin from Palacos® R+G was 1.148 µg/cm2, while Simplex® T released 198.87 µg/cm2 tobramycin over 14 days. Inhibition zone tests showed that Palacos® R+G maintained antibacterial activity for 42 days, while Simplex® T's activity diminished after 14 days. Statistical analysis confirmed significant differences in antibacterial efficacy between the two cements. Palacos® R+G demonstrated superior gentamicin release and sustained antibacterial activity compared to tobramycin from Simplex® T. These findings suggest that Palacos® R+G may offer better clinical outcomes in preventing and treating PJIs.
期刊介绍：
Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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