Microorganisms最新文献

{"title":"<i>Salmonella</i> Infection Induces Orchitis and Disrupts the Blood-Testis Barrier, Leading to Spermatogenic Disorders in Mice.","authors":"Yingchao Li, Qian Ma, Chenyang Shi, Qirui Zang, Yaolong Song, Mingshuai Chen, Binhuan Ma, Panpan Tong, Zhanqiang Su, Yi Zhang, Shicheng Wan, Aili Aierken, Mengfei Zhang","doi":"10.3390/microorganisms14081862","DOIUrl":"10.3390/microorganisms14081862","url":null,"abstract":"<p><p>This study investigated the pathological processes by which two <i>Salmonella</i> strains induce orchitis and impair spermatogenesis in mice, with emphasis on inflammation and blood-testis barrier (BTB) integrity. Thirty male Kunming mice were randomly assigned to the human-derived <i>Salmonella enterica</i> serovar Enteritidis H71 group, the sheep-derived <i>Salmonella enterica</i> serovar Agona W42 group, or the phosphate-buffered saline control group (n = 10 per group). An acute orchitis model was established by intrascrotal injection. Histopathological examination revealed marked testicular and epididymal lesions, disruption of the spermatogenic epithelium, and reduced sperm abundance in infected mice. Transcriptomic analysis identified 4546 differentially expressed genes shared by the two infected groups and showed enrichment of the Toll-like receptor (TLR), nuclear factor kappa B (NF-κB), and mitogen-activated protein kinase (MAPK) signaling pathways. Real-time quantitative PCR further showed increased expression of interleukin 6 (<i>Il6</i>), interleukin 1 beta (<i>Il1b</i>), and tumor necrosis factor (<i>Tnf</i>), accompanied by reduced expression of tight junction protein 1 (<i>Tjp1</i>), occludin (<i>Ocln</i>), and synaptonemal complex protein 3 (<i>Sycp3</i>) in infected mice (<i>p</i> < 0.05), except for <i>Tjp1</i> in the W42 group. These findings indicate that <i>Salmonella</i>-induced inflammatory activation is associated with BTB disruption and impaired spermatogenesis, providing a basis for further investigation of bacterial orchitis and zoonotic reproductive risks.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"14 8","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13515053/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148830363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Detection of <i>Blastocystis</i> ST2 and ST4 and Exploratory 16S rRNA Gene Community Profiles in a 12-Week Dairy-Intervention Pilot Study.","authors":"Panayiota Tsokkou, Chad Schou, Catherine O'Dowd Phanis, Kyriacos A Hasapis, Stella A Nicolaou, Eleni P Andreou, Evelina Charidemou","doi":"10.3390/microorganisms14081860","DOIUrl":"10.3390/microorganisms14081860","url":null,"abstract":"<p><p><i>Blastocystis</i> is a common intestinal protist whose clinical and ecological significance remains uncertain. This secondary exploratory analysis examined <i>Blastocystis</i> PCR detection in paired baseline and Week 12 stool samples collected during a randomized pilot intervention comparing daily goat-milk and cow-milk consumption. Nineteen healthy Greek Cypriot adults provided 38 stool samples. Stool DNA was screened by PCR targeting a partial 18S rRNA gene fragment, and positive amplicons were Sanger sequenced for subtype assignment. <i>Blastocystis</i> was detected at both time points in 3 of 19 participants (15.8%); ST2 was identified in two participants and ST4 in one. No <i>Cryptosporidium</i> spp. or <i>Giardia duodenalis</i> PCR-positive samples were identified. In exploratory 16S rRNA gene analyses, median Shannon diversity index was 6.10 versus 4.85 at baseline (<i>p</i> = 0.171) and 5.67 versus 4.17 at Week 12 (<i>p</i> = 0.085) in repeat-detection and no-detection groups, respectively. Bray-Curtis PERMANOVA was non-significant at baseline (<i>R</i>² = 0.0946, <i>p</i> = 0.0815) and Week 12 (<i>R</i>² = 0.0597, <i>p</i> = 0.8576); Aitchison-distance sensitivity analyses were likewise non-significant (<i>p</i> = 0.0547 and <i>p</i> = 0.3232, respectively). No selected genus-level comparison met the false-discovery-rate-adjusted threshold. The three-participant repeat-detection subgroup precluded reliable analysis of dairy-arm effects, subtype-specific differences, cardiometabolic outcomes, or bacterial-community associations. In this small pilot cohort, ST2 and ST4 were identified in paired baseline and Week 12 stool samples from three healthy adults, providing molecular evidence of repeat detection over the sampled interval. Larger studies with more frequent sampling, quantitative assays, and adequately powered, prespecified analyses are needed to investigate the temporal dynamics and host-associated correlates of <i>Blastocystis</i> detection.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"14 8","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13515294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148830384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Collection Efficiency of a Wet-Type Electrostatic Precipitator for Aerosolized Influenza A Virus.","authors":"Kazuya Nakamura, Takeshi Nagai, Hitoshi Ishiguro, Keiichi Kobayashi, Kazuhisa Nakagawa, Masahiro Okanojo, Akira Nukazuka","doi":"10.3390/microorganisms14081863","DOIUrl":"10.3390/microorganisms14081863","url":null,"abstract":"<p><p>Airborne viruses are a key driver of infectious disease transmission, highlighting the importance of reliable detection in public health surveillance. As atmospheric viral concentrations are very low, a sampler with a high viral collection efficiency is essential. Although multiple approaches for evaluating collection efficiency have been applied using various samplers, no standardized sampler has yet been developed. We previously developed a wet-type electrostatic precipitator (WT-ESP) and successfully collected severe acute respiratory syndrome coronavirus 2 from the public environment. However, its efficiency for quantitative collection of airborne viruses remains unclear. This study aimed to clarify the collection efficiency of the WT-ESP. We evaluated collection efficiency via two different approaches: direct spray, where virus-containing aerosols were sprayed directly into the WT-ESP inlet, and indirect spray, where aerosols were dispersed into a closed space and then collected using the sampler. The direct spray tests achieved 20.1-50.2% collection efficiency, whereas the indirect spray test achieved an efficiency <12%. These findings highlight that electrostatic precipitation has an advantage of enhancing collection efficiency compared with values reported for impingers in previous studies and provide preliminary insights into the collection efficiencies under direct and indirect spray conditions, providing foundational data that bridge the gap between both methods.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"14 8","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13515792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148830301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatincolaceae (Alphaproteobacteria) Symbionts of Snapping Shrimp <i>Alpheus brevicristatus</i>: Genomic Capacity for Functions Beyond Nutrient Scavenging.","authors":"Fang-Chao Zhu, Yan-Bin Yang, Pei-Pei Liu, Xin Liu, Qun-Jian Yin, Xu-Yang Chen, Shuo Yu","doi":"10.3390/microorganisms14081864","DOIUrl":"10.3390/microorganisms14081864","url":null,"abstract":"<p><p><i>Candidatus</i> Hepatincolaceae is a poorly characterized family of obligate Alphaproteobacterial symbionts that are widely detected in ecdysozoans. They were previously assumed to play a nutrient-scavenging role in the gut lumen. In this study, two high-quality metagenome-assembled genomes (MAGs, 1.39 Mb and 1.48 Mb in size) were recovered from the gut of the snapping shrimp <i>Alpheus brevicristatus</i> via metagenomic sequencing. Phylogenetic and whole-genome similarity analyses confirm that these two MAGs represent two novel, undescribed genera within the family <i>Ca</i>. Hepatincolaceae. Metabolic reconstruction reveals that they not only retain the canonical nutrient-scavenging pathways conserved across all Hepatincolaceae members, but also encode previously undocumented functional modules for antioxidant defense, vitamin B₁ and B₂ biosynthesis, and short-chain fatty acid production. They maintain a high oxygen-affinity cytochrome bd terminal oxidase to thrive in the anoxic gut microenvironment. Consistent with their symbiotic lifestyle, their genomes exhibit typical signatures of reductive evolution, such as reduced genome size, low GC content, and gene loss in amino acid and nucleotide de novo biosynthesis pathways. This study presents the first reported high-quality genomes of marine <i>Ca</i>. Hepatincolaceae symbionts, which are predicted to possess multiple metabolic functions extending beyond nutritional mutualism.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"14 8","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13515547/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148830179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal Infections Associated with Sphingosine 1-Phosphate Receptor Modulators: Immunological Mechanisms, Clinical Patterns, and Management Considerations.","authors":"Kian Michael Yazdan, Sai Tapasvi Madam, Shirisha Pasula","doi":"10.3390/microorganisms14081861","DOIUrl":"10.3390/microorganisms14081861","url":null,"abstract":"<p><p>Fungal infections constitute a large portion of serious infections worldwide and are increasing, partially due to new immunomodulatory therapies, such as Sphingosine-1-Phosphate (S1P) receptor modulators, including fingolimod for the treatment of multiple sclerosis (MS). Fingolimod antagonizes multiple S1P receptors, including S1PR₁, S1PR₃, S1PR₄, and S1PR₅, resulting in immunological alterations that may increase susceptibility to invasive fungal infections (IFIs). Our PubMed-based literature search identified 43 published cases and a 60-patient case series describing invasive fungal infections in patients receiving S1P receptor modulators. Of these, cryptococcosis was the most frequently documented infection, followed by histoplasmosis and coccidioidomycosis. Most published cases involved patients on fingolimod, whereas evidence for the other medications in this class is extremely limited. Currently, there are no standardized fungal screening protocols. A baseline complete blood count with differentials should be obtained before the initiation of S1P receptor modulator therapy. Patients should also be monitored clinically for signs and symptoms of fungal infection. Primary antifungal prophylaxis and routine serum cryptococcal antigen testing are not currently recommended. Management of suspected IFIs should focus on diagnostic workup to identify the causative organism and extent of organ involvement to determine the appropriate antifungal therapy. Decisions regarding interruption or discontinuation of MS therapy should be individualized according to the clinical syndrome, infection severity, and risk of MS rebound. This narrative review synthesizes the available mechanistic and clinical evidence on invasive fungal infections in patients with multiple sclerosis receiving S1P receptor modulator therapy.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"14 8","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13515163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148830361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BTV Remodels the oISG15-Association Proteome.","authors":"Di Kang, Qinxue Gao, Mingxin Zhang, Rui Ha, Xinbing Hu, Meng Li, Zhongming Chen, Shijun Bao","doi":"10.3390/microorganisms14081855","DOIUrl":"10.3390/microorganisms14081855","url":null,"abstract":"<p><p>ISG15 is an interferon-induced ubiquitin-like protein that exerts diverse functions during viral infection. We previously reported that ovine ISG15 (oISG15) promoted bluetongue virus (BTV) replication by stabilizing viral NS1 and VP4 in an ISGylation-independent manner, although the mechanism remained unknown. Here, using the generated anti-oISG15 antibody, we performed immunoprecipitation (IP) coupled with label-free Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) to characterize the oISG15-associated proteome in BTV-infected and uninfected cells. The results showed that BTV infection markedly remodeled the oISG15 association landscape, with decreased enrichment of several autophagy- and trafficking-related proteins and increased enrichment of mitochondrial metabolic proteins in oISG15 immunoprecipitates. Functionally, oISG15 overexpression induced modest alterations in autophagy-related markers in uninfected cells yet attenuated these markers' abundance during BTV infection. Together, this study revealed BTV-induced changes in the oISG15-associated proteome that coincided with alterations in autophagy-related markers.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"14 8","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13515662/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148829731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, Biocontrol Efficacy and Genomic Analysis of <i>Trichoderma harzianum</i> Snef3255 Against Peanut Root-Knot Nematode Disease.","authors":"Xuesong Bai, Zhigang Li, Chenjun Duan, Di Zhao, Hai Dong, Xiaofeng Zhu, Haiyan Fan, Xiaoyu Liu, Yuxi Duan, Lijie Chen","doi":"10.3390/microorganisms14081851","DOIUrl":"10.3390/microorganisms14081851","url":null,"abstract":"<p><p>Peanut root-knot nematode (RKN) disease caused by <i>Meloidogyne hapla</i> is among the most destructive soil-borne diseases threatening peanut production worldwide. We isolated <i>Trichoderma harzianum</i> strain Snef3255 from peanut rhizosphere soil, which exhibited high virulence against second-stage juveniles, with a mortality rate of 86.08%. Field trials demonstrated that TC and TS provided control efficacies of 70.85% and 61.88%, respectively, while boosting peanut yields by 18.15% and 13.61%. We report a high-quality chromosome-level genome assembly of <i>T. harzianum</i> Snef3255 using Oxford Nanopore, Illumina and high-throughput chromatin conformation capture (Hi-C) sequencing data. This assembly comprised seven chromosomes (40,811,729 bp) with a BUSCO completeness of 99.31%. A total of 13,611 protein-coding genes were predicted. Systematic comparative genomic analysis of <i>T. harzianum</i> Snef3255 with other <i>Trichoderma</i> species was performed, and putative functional gene clusters were investigated. Furthermore, we identified two candidate secreted proteins, ThCP1 and ThLysM1, and screened their interacting proteins in peanut, respectively. This study demonstrated the potential of Snef3255 as a biocontrol agent for peanut RKN disease and provided a genomic basis for understanding <i>Trichoderma</i>-peanut interactions.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"14 8","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13515719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148830119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined Plasmid Redesign and Transfection Optimization Significantly Increases Upstream AAV Titers While Maintaining Vector Quality and In Vivo Potency.","authors":"Shiliang Hu, Yinxing Chen, Carmen Wu, Wilhad Hans Reuter, June Deng, Nannan Jia, Noel Walsh, Amy Bastille, Thomas M Edwards, Matthias Hebben, Nelson Chau, Jing Liao","doi":"10.3390/microorganisms14081857","DOIUrl":"10.3390/microorganisms14081857","url":null,"abstract":"<p><p>A high manufacturing cost of goods (CoG) remains a critical barrier to the broad clinical adoption of gene therapies and is driven in part by limited productivity in adeno-associated virus (AAV) manufacturing. Here, we report an optimized AAV production process developed to markedly increase upstream titers while preserving vector quality and potency. The process combines a redesigned plasmid system, an optimized plasmid ratio, and a novel synthetic transfection reagent and was benchmarked against a conventional triple-plasmid/PEI MAX workflow. Across multiple AAV capsids and independent production runs, the optimized process reproducibly increased crude harvest titers by approximately 10- to 33-fold relative to the standard process, while maintaining key vector quality attributes. Notably, within the detection limits of the assay, rcAAV was undetectable at 1 × 10¹⁰ vg input with the optimized process, whereas the conventional triple-plasmid (with native Rep-Cap sequence)/PEI MAX workflow remained rcAAV-positive under identical conditions. Importantly, the in vivo potency was comparable to that of vectors produced by the conventional process. These results position our optimized AAV production process as a promising strategy to materially reduce AAV manufacturing CoG per patient.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"14 8","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13515234/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148830245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Itaconic Acid Directly Binds to the Cysteine Residue of PurF: A Novel Mechanism for Inhibiting Purine Metabolism in Porcine Pathogenic <i>Escherichia coli</i>.","authors":"Haozhen Liu, Xin Li, Xinyu Zhang, Yao Ge, Yinfeng Chen, Xinjian Li, Zhenlong Wu","doi":"10.3390/microorganisms14081852","DOIUrl":"10.3390/microorganisms14081852","url":null,"abstract":"<p><p>Itaconic acid has been reported to possess anti-inflammatory and antibacterial properties. However, its specific mechanisms of action against pathogenic bacteria, especially in the context of purine metabolism, remain poorly understood. Here, we investigate the impact of itaconic acid on the purine metabolism of PCN033, a highly pathogenic porcine extraintestinal pathogenic <i>Escherichia coli</i> (ExPEC) strain. Our in vitro and in vivo experiments demonstrated that itaconic acid significantly inhibited the proliferation of PCN033. Multi-omics analyses, including transcriptome and metabolome sequencing, revealed that itaconic acid severely disrupted the purine metabolism pathway of PCN033. Further mechanistic studies identified PRPP amidotransferase (PurF), a key enzyme in de novo purine synthesis, as a direct target of itaconic acid. Molecular docking and click chemistry experiments provided compelling evidence that itaconic acid specifically binds to the second cysteine residue (2C) of PurF, leading to the inhibition of its enzymatic activity. These data reveal a novel and critical mechanism by which itaconic acid exerts its antibacterial effects in response to bacterial infection. Importantly, our in vivo data show that supplementation with itaconic acid alleviated weight loss, organ damage, and inflammatory responses induced by PCN033 infection in mice and nursery pigs. These novel findings enhance our understanding of the antibacterial mechanisms of itaconic acid. Supplementation with itaconic acid could serve as a therapeutic strategy for treating pathogenic bacterial infections in humans and other animals.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"14 8","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13515712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148830314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial Diversity and Hydrocarbon-Oxidizing Bacteria in Coastal Waters and Sands Contaminated by the Fuel Oil Spill in the Black Sea.","authors":"Ekaterina M Semenova, Alexey P Ershov, Tamara L Babich, Diyana S Sokolova, Nataliya G Loiko, Elena A Bakay, Ekaterina S Kazak, Tamara N Nazina","doi":"10.3390/microorganisms14081856","DOIUrl":"10.3390/microorganisms14081856","url":null,"abstract":"<p><p>In 2024, an accident involving two tankers in the Kerch Strait resulted in the release of approximately 2400 tons of fuel oil into the Black Sea, causing significant contamination of seawater and the coastal zone. This study presents the first microbiological and molecular-ecological assessment of prokaryotic community composition and hydrocarbon-oxidizing bacteria (HOB) in coastal seawater and sand near Anapa (Russian Federation) following the spill. The taxonomic composition of nine samples was analyzed using high-throughput sequencing of 16S rRNA genes (V3-V4 regions), identifying <i>Bacteria</i> as the dominant domain (85.3-99.8%). In seawater samples, bacteria of the phyla <i>Pseudomonadota</i>, <i>Cyanobacteriota</i>, and <i>Bacteroidota</i> and archaea of the phyla <i>Thermoplasmatota</i> and <i>Crenarchaeota</i> predominated. Eighteen aerobic bacterial strains, including members of the genera <i>Shewanella</i>, <i>Pseudoalteromonas</i>, <i>Halopseudomonas</i>, <i>Marinomonas</i>, <i>Pseudomonas</i>, <i>Vibrio</i>, <i>Alcanivorax</i>, <i>Ectopseudomonas</i>, <i>Nitratireductor</i>, and <i>Echinicola,</i> were isolated from the zone of fuel oil spill. Several isolates demonstrated heavy oil degradation and biosurfactant production. Screening of collection strains isolated from other habitats revealed that <i>Rhodococcus erythropolis</i> TG65 and <i>Marinobacter lutaoensis</i> Pd1 and Pd2 degraded 92-94% of fuel oil <i>n</i>-alkanes. Elevated dissolved iron concentrations in the seawater indicate the possibility of a metabolic coupling between hydrocarbon oxidation and microbial iron reduction, mediated by indigenous <i>Shewanella</i> and <i>Pseudomonas</i> species. These findings indicate that indigenous HOB may contribute to the natural attenuation of aliphatic hydrocarbons in fuel oil.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"14 8","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13515116/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148830381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}