Microorganisms最新文献

{"title":"In-Store Cosmetic Testers Harbour Resistant Bacteria: A Pilot Survey of Microbial Contamination and Antimicrobial Susceptibility in Portuguese Pharmacies.","authors":"Sónia Ribeiro, Jéssica Duarte Silva, Patrícia Pacheco, Célia Fortuna Rodrigues","doi":"10.3390/microorganisms14092120","DOIUrl":"10.3390/microorganisms14092120","url":null,"abstract":"<p><p>Cosmetic testers in community pharmacies may act as a source of microbial transmission, but their microbiological safety remains poorly characterised. This study assessed microbial contamination of 42 cosmetic testers (eight product categories) from community pharmacies in northern Portugal using culture-based methods, identification and Kirby-Bauer disk diffusion. We characterised the antimicrobial susceptibility of the recovered isolates. Microbial growth was detected in 25/42 testers (59.5%), with polymicrobial contamination in 16.7% of samples. Out of the 25 positive growth products, 33 characterised bacterial isolates were yielded. <i>Staphylococcus</i> sp. predominated (22/33; 66.7%), followed by <i>Pseudomonas</i> sp. (4/33; 12.1%), <i>Streptococcus</i> sp. (3/33; 9.1%), <i>Acinetobacter</i> sp. (2/33; 6.1%) and <i>Bacillus</i> sp. (2/33; 6.1%). Among categories represented by more than one tester, anti-ageing products showed the highest proportion of microbial growth (7/8; 87.5%), followed by sunscreens (10/16; 62.5%). Overall, 12/33 bacterial isolates (36.4%) met the study-defined operational multidrug-resistance criterion, all of which were <i>Staphylococcus</i> sp. (12/22; 54.5%). These findings demonstrate that viable bacteria exhibiting phenotypic antimicrobial resistance can be recovered from in-use cosmetic testers in community pharmacies and support further investigation of their microbiological and antimicrobial-resistance characteristics. This reinforces that pharmacy cosmetic testers may harbour a diverse, and often multidrug-resistant, microbial population of clinical relevance, supporting the need for strengthened tester hygiene and control practices.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"14 9","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13609243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149062748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Planococcus terreus</i> sp. nov. and <i>Planococcus flavus</i> sp. nov., Two New Species Isolated from Soil of the Napahai Plateau Wetland.","authors":"Weidong Lu, Xingfeng Qian, Zheng Zhang, Zhiying Li, Zefen Yu, Yunxia Tian, Li Li, Yongxia Wang","doi":"10.3390/microorganisms14092119","DOIUrl":"10.3390/microorganisms14092119","url":null,"abstract":"<p><p>Two novel bacterial strains, designated YIM B11945^T and YIM B11997^T, were isolated from soil samples collected in the Napahai Plateau wetland, Yunnan Province, China. The cells were aerobic, Gram-stain-positive, motile and non-spore-forming rods. The strains YIM B11945^T and YIM B11997^T showed the highest 16S rRNA sequence similarity with <i>Planococcus shixiaomingii</i> N022^T (97.5%) and <i>Planococcus koreensis</i> JG07^T (98.3%), respectively. The phylogenomic analyses based on the core genome sequences placed the two strains within the genus genus <i>Planococcus</i>. The average nucleotide identity and the digital DNA-DNA hybridization values between the two novel strains and their closest relatives were below the species delineation thresholds of 95% and 70%, respectively. The strains YIM B11945^T and YIM B11997^T possessed major fatty acids, which were anteiso-C_15:0 and C_16:1ω7c alcohol, and anteiso-C_15:0, iso-C_14:0, C_16:1ω7c alcohol and iso-C_16:0, respectively. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, and diphosphatidylglycerol, and the respiratory quinones detected were menaquinone-7 and menaquinone-8. The genomic DNA G+C contents of YIM B11945^T and YIM B11997^T were 45.0% and 49.2%, respectively. Based on the phenotypic, genotypic and phylogenetic analyses, the strains YIM B11945^T and YIM B11997^T are considered to represent two new species of the genus <i>Planococcus</i>, for which the names <i>Planococcus terreus</i> sp. nov. and <i>Planococcus flavus</i> sp. nov. are proposed, respectively. The type strains are YIM B11945^T (=CGMCC 1.64737^T=KCTC 43683^T) and YIM B11997^T (=KCTC 43680^T=NBRC 116630^T).</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"14 9","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13609399/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149062568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic Assembly and Multifunctional Roles of Plant Endophytic Microbiomes.","authors":"Jie Song, Wenhui Liu, Yan Sun, Jinfeng Shi, Meiqi Liu, Yuting Dai, Yuhu Zuo, Qin Yao","doi":"10.3390/microorganisms14092118","DOIUrl":"10.3390/microorganisms14092118","url":null,"abstract":"<p><p>Healthy host plants harbor taxonomically structured and diverse endophytic microbial communities that establish sophisticated symbiotic crosstalk with their hosts. These endophytic microbiomes confer multiple beneficial traits, including growth promotion, nutrient acquisition, and enhanced resistance to biotic and abiotic stresses, and are increasingly recognized as key modulators of plant fitness. The assembly of endophytic communities is not random but shaped by combined effects of environmental cues, host filtering, and microbial-microbial interactions, among which plant immunity constitutes an important host-selection dimension. Beneficial endophytes deploy diverse molecular tactics, such as masking microbe-associated molecular patterns (MAMPs) and secreting immune-suppressive compounds, to evade host PAMP-triggered immunity and effector-triggered immunity (PTI-ETI) surveillance for persistent internal colonization. In this review, based on the literature retrieved from Web of Science Core Collection and Scopus (2010-2026), we systematically summarize the colonization process, dynamic assembly rules, and driving factors of plant endophytic microbiomes. We further elaborate their multifaceted physiological functions in regulating plant growth, nutrient utilization, and stress adaptation. Deciphering such multilayered plant-endophyte interactions provides important insights for harnessing beneficial endophytes to advance sustainable agricultural development.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"14 9","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13609686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149062738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and GC-MS-Based Identification of Possible PVC Degradation Pathways of Bacteria for Sustainable Microplastic Bioremediation.","authors":"Nadia Hussain, Alishba Saif, Fatima Muccee, Muhammad Imran Afzal, Wardah Shahid, Amal H I Al Haddad","doi":"10.3390/microorganisms14092114","DOIUrl":"10.3390/microorganisms14092114","url":null,"abstract":"<p><p>Polyvinyl chloride (PVC), due to its persistent nature and the hazardous effects associated with chlorine content, poses global challenges like accelerated environmental pollution, public health crises and an increased economic burden. Its sustainable decontamination from the environment could be possible with the assistance of bacteria. The current study was initiated to achieve this sustainable goal. PVC-degrading bacteria were isolated via the serial dilution method using minimal salt medium (MSM) supplemented with PVC as a sole carbon source and characterized via 16S RNA gene sequencing analysis, growth curve and PVC strip weight loss assays, antibiotic sensitivity profiling, FTIR and GC-MS-based metabolic profiling. Five isolates, <i>Achromobacter xylosoxidans</i> SBBPVC1, <i>Bacillus licheniformis</i> SBBPVC2, <i>Pseudomonas citronellolis</i> SBBPVC3, <i>P. stutzeri</i> SBBPVC4 and <i>Bacillus flexus</i> SBBPVC5 were identified. All were fast-growing, with PVC degradation efficiency ranging between 20-50% per 30 days. The FTIR spectral comparison with the control clearly indicated peak variations in regions 1100-1250, 1370, 1460 and 1720-1740 cm^-1, corresponding to C-O and C-C, CH₃, CH₂ and C=O bond vibrations, validating PVC degradation in the presence of bacteria. GC-MS-based identification of four esters, i.e., 1-methyl heptyl, isooctyl, adipic acid, methyl octyl and octyl myristate esters, along with aliphatic alcohols and aldehydes, confirmed the degradation of PVC and its use as a carbon source by bacteria. Bacteria explored in current study could be used as bioremediating agents for PVC decontamination of the environment.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"14 9","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13609082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149062604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>M. tuberculosis</i> Beijing Genotype Clusters: Impact on Tuberculosis Drug Resistance in Kazakhstan.","authors":"Ainur Akhmetova, Zhannur Abilova, Dauren Yerezhepov, Lyailya Chingissova, Ainur Akilzhanova, Ulan Kozhamkulov","doi":"10.3390/microorganisms14092121","DOIUrl":"10.3390/microorganisms14092121","url":null,"abstract":"<p><p>The Beijing genotype is the main component of the <i>M. tuberculosis</i> population in Kazakhstan. In this study, we investigated the association of drug resistance (including MDR) and clinical forms of tuberculosis with the Beijing genotype and its dominant clusters, and compared obtained results among new, recurrent and all TB cases. In total, 759 <i>M. tuberculosis</i> isolates were collected from new (<i>n</i> = 540) and recurrent TB cases (<i>n</i> = 219). Drug susceptibility testing was performed on the BACTEC-MGIT 960 system; 24 MIRU-VNTR analysis was carried out to identify <i>M. tuberculosis</i> genotypes. The Beijing genotype was detected in 65.2% (495/759) of all TB cases and was associated with recurrent TB compared with new cases (78% vs. 60%, <i>p</i> < 0.0001). Among both new and recurrent TB cases, the Beijing genotype was associated with drug resistance (<i>p</i> < 0.0001 and <i>p</i> < 0.0001), including MDR (<i>p</i> < 0.0001 and <i>p</i> = 0.0118). Central Asian/Russian cluster 94-32 was the most widespread Beijing cluster in Kazakhstan (52.5%; 260/495). Association of cluster 94-32 with drug-resistant TB in general was confirmed across all cases (<i>p</i> = 0.010) and recurrent TB cases (<i>p</i> = 0.0191; OR = 4.8958; 95%CI: 1.2965-18.4883), while individuals with new TB cases were more likely to be diagnosed with MDR-TB specifically (<i>p</i> = 0.021). Among clinical forms of TB, a significant association was detected between fibrous-cavernous tuberculosis and drug resistance (<i>p</i> = 0.0019; OR = 3.3801; 95%CI: 1.5692-7.2807), including MDR (<i>p</i> = 0.0054; OR = 3.2453; 95%CI: 1.4167-7.4345), among all TB cases. TB patients with Beijing cluster 94-15 mostly had drug-susceptible TB among all TB cases and recurrent cases (<i>p</i> < 0.05). Drug-susceptible tuberculosis was observed among patients with focal TB among new cases (<i>p</i> = 0.0254; OR = 0.1723; 95%CI: 0.0369-0.8050). Tuberculous pleurisy was more commonly diagnosed in individuals with non-Beijing genotypes among all TB cases (<i>p</i> = 0.0430; OR = 0.3643; 95%CI: 0.1371-0.9686). The <i>M. tuberculosis</i> Beijing genotype, particularly the Central Asian cluster 94-32, plays a significant role in the distribution of drug-resistant TB among all TB cases and recurrent cases, including MDR-TB among new TB cases in Kazakhstan. On the contrary, the Beijing cluster 94-15 may contribute to the spread of drug-susceptible TB among all TB cases and recurrent TB cases.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"14 9","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13609652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149062658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Field-Scale Suppression of Cyanobacterial Blooms by Cationic Polymer-Modified Local Soil: Selective Inactivation Mechanism and Ecological Responses.","authors":"Ningyan Peng, Yuanyuan Fang, Ping Yang, Jin Liu, Yunlu Jia, Zhengwei Dai, Xiaojin Zhang, Renjie Zhao, Shouchun Li, Guofei Dai","doi":"10.3390/microorganisms14092111","DOIUrl":"10.3390/microorganisms14092111","url":null,"abstract":"<p><p>Harmful cyanobacterial blooms require control strategies that suppress bloom-forming taxa while limiting effects on non-target primary producers and toxin-related risks. We developed a cationic polyquaternium (P126)-engineered local red-soil composite and evaluated its electrokinetic properties, taxon-dependent photophysiological effects, activity against colonial <i>Microcystis</i>, robustness to water chemistry, short-term <i>Danio rerio</i> responses, and field-scale performance. P126 modification reversed soil surface charge to the range of +20 to +33 mV across a pH range of 6.0-10.0, generating a strong electrostatic contrast with negatively charged cyanobacteria. At 10 mg L^-1, the composite suppressed maximum photosystem II quantum yield (F_v/F_m) in <i>Microcystis aeruginosa</i> and <i>Dolichospermum</i> sp., with no statistically detectable effects on the four tested chlorophyte and diatom strains. For <i>Microcystis</i> colonies ≥ 500 μm, 20 mg L^-1 of composite achieved >95% MTT-based inhibition within 24 h, versus < 15% for CuSO₄. Suppression persisted across the tested pH and nutrient ranges but was attenuated by high humate concentrations. The maximum unfractionated-sample ELISA microcystin-equivalent signal was approximately 25% lower than with CuSO₄; however, dissolved and particulate fractions were not resolved. No statistically detectable treatment-related differences in zebrafish hatching or larval length were observed up to 10.5 mg L^-1. In a physically isolated sub-lake, composite application preceded a decline of more than three orders of magnitude in algal/cyanobacterial density and an increase in Secchi depth from approximately 0.25 to 1.2 m; <i>Vallisneria natans</i> was planted subsequently. These results provide laboratory evidence and field case-study observations consistent with electrostatically favored, selective bloom suppression, while residual-polymer fate, sediment effects, chronic toxicity, and multi-trophic responses require further evaluation.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"14 9","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13609796/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149062707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virotherapy for Spinal and Spinal Cord Tumors: Current Evidence and Future Perspectives.","authors":"Koji Uotani, Tomohiro Fujiwara, Ryo Takatori, Kazutaka Yamashita, Kenzaburo Matsumoto, Yoshiaki Oda, Kensuke Shinohara, Hiroshi Tazawa, Toshiyoshi Fujiwara, Toshifumi Ozaki","doi":"10.3390/microorganisms14092110","DOIUrl":"10.3390/microorganisms14092110","url":null,"abstract":"<p><p>Tumors of the spine and spinal cord, including primary and metastatic vertebral tumors, intramedullary gliomas, and refractory intradural lesions, are challenging to treat because curative local therapy is limited by the eloquent, nonregenerating neural tissue surrounding them. Oncolytic virotherapy may offer a rational alternative by combining tumor-selective oncolysis with the induction of systemic antitumor immunity, while sparing normal neural cells. This review summarizes the current field of oncolytic viruses, including adenovirus, herpes simplex virus, reovirus, and others, in the context of spinal and spinal cord tumors. Clinical data in the spine remain scarce; however, the rationale is based on histological evidence from sarcomas and other tumors, extensive glioma experience, including diffuse intrinsic pontine glioma, and preclinical activity in nerve sheath and meningioma models. We discuss the telomerase-specific oncolytic adenovirus OBP-301 and its derivatives, whose <i>hTERT</i>-driven replication provides histology-agnostic tumor selectivity, while sparing the telomerase-silent spinal cord. This telomerase dependence, however, limits activity against tumors that maintain telomeres through the alternative lengthening of telomeres (ALT) pathway, such as many osteosarcomas and some spinal cord astrocytomas. We also discuss delivery and safety within the confined spinal canal, combination strategies, and future directions, such as extracellular vesicle-mediated delivery and biomarker-guided patient selection. Although clinical translation to the spine will require dedicated preclinical and early-phase studies, virotherapy represents a promising, mechanistically grounded modality for these therapeutically challenging tumors.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"14 9","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13609713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149062805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution Characteristics of Antimicrobial-Resistance and Virulence-Associated Genes of <i>Bacillus cereus</i> in Animal-Origin Food in Guangzhou, China.","authors":"Weiqi Liu, Wanxin Lin, Zhengyuan He, Peng Wan, Juntao Li, Wenguang Xiong, Zhenling Zeng","doi":"10.3390/microorganisms14092116","DOIUrl":"10.3390/microorganisms14092116","url":null,"abstract":"<p><p>This study investigated the prevalence and genomic characteristics of Bacillus cereus group organisms in animal-origin foods sold in Guangzhou, China. A total of 505 retail samples, including 117 milk samples and 388 commercially available smoked sausage products, were collected from supermarkets and farmers' markets. The isolation rates of <i>B. cereus</i> group organisms were 8.5% in milk and 44.9% in smoked sausages. Whole-genome sequencing of 169 isolates showed that antimicrobial-resistance genes, including bacII, fosB and vanZF, and diarrheal virulence-associated genes, including nheA, nheB and nheC, were widely distributed among the isolates. Most representative isolates showed biofilm-forming ability, and three antimicrobial-resistant isolates carrying multiple virulence-associated genes showed pathogenic potential in mice under high-dose experimental administration. These findings indicate that retail smoked sausage products can serve as a reservoir of <i>B. cereus</i> group organisms with antimicrobial-resistance, virulence-associated and biofilm-forming traits. However, the detection of resistance- or virulence-associated genes does not necessarily prove gene expression, toxin production, phenotypic resistance or consumer exposure risk. This study did not directly evaluate growth of <i>B. cereus</i> in sausage matrices during storage. Therefore, the present results indicate a potential microbiological hazard rather than a quantified consumer risk. Product-specific challenge studies under defined storage conditions are required to determine whether <i>B. cereus</i> can proliferate in particular sausage products and reach hazardous levels at consumption.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"14 9","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13609341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149062690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging and Underdetected Colonization Factors in Enterotoxigenic <i>Escherichia coli</i> (ETEC).","authors":"Astrid von Mentzer","doi":"10.3390/microorganisms14092123","DOIUrl":"10.3390/microorganisms14092123","url":null,"abstract":"<p><p>Adhesionto the gut epithelium is the first step in ETEC infection and is mediated by colonization factors (CFs). CFs are also the main targets of the ETEC vaccines currently in development, which are based on a small set of CFs considered to be the most prevalent. However, a large proportion of clinical isolates are reported as CF-negative when screened with standard PCR panels. This is partly because ETEC adhesins are more diverse than previously appreciated, and partly because the methods used to detect them only screen for a small, historically defined set of CFs. These two explanations are difficult to separate, and both suggest that CF diversity, and with it the coverage of current CF-based vaccines, has likely been underestimated. This review examines emerging and underdetected CFs in human ETEC, focusing on κ- and γ-clade adhesins such as CS23 and their links to animal-associated fimbriae. It discusses the implications of this growing diversity for vaccine development, the need for continued surveillance as the CF landscape evolves, and approaches to achieving broader vaccine coverage across the expanding diversity of ETEC adhesins.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"14 9","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13609481/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149062628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil Microeukaryote Diversity in the Crop Fields of Panama.","authors":"Mariel Monrroy, Priscila Moraga-Suazo, Regis Le-Feuvre, José Renán García","doi":"10.3390/microorganisms14092124","DOIUrl":"10.3390/microorganisms14092124","url":null,"abstract":"<p><p>Soil microeukaryotic communities are essential for nutrient cycling and the maintenance of soil fertility, yet their diversity in tropical agricultural systems remains poorly characterized. In this study, high-throughput metabarcoding of the V4 region of the 18S rRNA gene was used to characterize soil microeukaryotic communities across three agricultural regions of Panama. A total of 7714 unique operational taxonomic units (OTUs) were detected in composite soil samples collected from 44 sampling sites. The high-altitude region showed significantly greater species richness and distinct community composition compared with the lowland regions. Principal coordinate analysis identified two major clusters separating the high-altitude and lowland regions. Correlation analyses revealed associations between eukaryotic diversity and soil properties, particularly phosphorus content, electrical conductivity, and terrain slope. Taxonomic assignments, particularly at the species level, should be interpreted cautiously because they are based on 18S rRNA gene metabarcoding and OTU-based classification. These findings contribute to the characterization of soil microeukaryotic diversity in tropical agricultural fields and may support the integration of biodiversity assessments into soil management.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"14 9","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13609621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149062692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}