Microorganisms最新文献

{"title":"Genetic Diversity in Phytoplasmas from X-Disease Group Based in Analysis of <i>idpA</i> and <i>imp</i> Genes.","authors":"Florencia Ivette Alessio, Vanina Aylen Bongiorno, Carmine Marcone, Luis Rogelio Conci, Franco Daniel Fernandez","doi":"10.3390/microorganisms13051170","DOIUrl":"10.3390/microorganisms13051170","url":null,"abstract":"<p><p>Phytoplasmas of the X-disease group (16SrIII) are economically significant pathogens in South America, causing severe crop losses. Traditional classification based on the 16S rRNA gene has limitations in resolving closely related strains, prompting the exploration of alternative markers. This study focuses on the immunodominant membrane proteins imp and idpA, which exhibit high variability and play crucial roles in host-pathogen interactions. Through molecular characterization of <i>imp</i> and <i>idpA</i> genes in 16SrIII subgroups, we identified significant genetic diversity and distinct evolutionary pressures. The <i>imp</i> gene, under positive selection, showed high variability in its hydrophilic extracellular domain, suggesting adaptation to host immune responses. In contrast, <i>idpA</i> exhibited strong negative selection, indicating functional conservation. Phylogenetic analyses revealed that <i>imp</i> and <i>idpA</i> provide higher resolution than the 16S rRNA gene, enabling finer differentiation within subgroups. These findings highlight the potential of <i>imp</i> and <i>idpA</i> as complementary markers for phytoplasma classification and diagnostics.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114192/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144159892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Phosphogypsum on Viability of <i>Trichuris suis</i> Eggs in Anaerobic Digestion of Swine Manure.","authors":"Olexandra Boyko, Viktor Brygadyrenko, Yelizaveta Chernysh, Viktoriia Chubur, Hynek Roubík","doi":"10.3390/microorganisms13051165","DOIUrl":"10.3390/microorganisms13051165","url":null,"abstract":"<p><p>Waste from livestock farms contains various pathogens, including eggs and larvae of helminths-pathogens of parasitic diseases harmful to animals and humans. One of the methods for their effective processing to obtain biofertilizer and biofuel is anaerobic digestion, which requires further improvement to completely suppress the viability of pathogenic microorganisms in mesophilic conditions. To this end, the use of anaerobic digestion under sulfate reduction conditions to suppress pathogens using biogenic hydrogen sulfide is promising. Consequently, this study aims to study the effect of a sulfur-containing additive such as phosphogypsum on the disinfection of pig manure during anaerobic digestion. Egg mortality was already found to increase significantly compared to the control (80% and more), even at a minimum concentration of phosphogypsum (5%), on the fifth day of the experiment. At the same time, the maximum effect (100% mortality of <i>Trichuris suis</i> eggs) was recorded at a 10% concentration of phosphogypsum, starting from the 10th day of the study. Our experiment showed that changes in anaerobic digestion conditions using phosphogypsum could positively affect digestate disinfection. However, further research is needed to optimize the conditions of the process for an effective combination of disinfection with the production of environmentally safe organic fertilizers and high-quality biogas with a high level of methane.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of Phenanthrene by <i>Mycobacterium</i> sp. TJFP1: Genetic Basis and Environmental Validation.","authors":"Shuyun Li, Jiazhen Liu, Ping Fang","doi":"10.3390/microorganisms13051171","DOIUrl":"10.3390/microorganisms13051171","url":null,"abstract":"<p><p>The development of efficient bioremediation technologies for polycyclic aromatic hydrocarbons contamination is a hot research topic in the environmental field. In this study, we found that the <i>Mycobacterium</i> sp., TJFP1, has the function of degrading low molecular weight PAHs, and further investigated its degradation characteristics using the PAH model compound phenanthrene as a target pollutant. The optimal growth and degradation conditions were determined by single-factor experiments to be 37 °C, pH 9.0, and an initial concentration of 100 mg/L phenanthrene. Under this condition, the degradation efficiency of phenanthrene reached 100% after 106 h of incubation, and the average degradation rate could reach 24.48 mg/L/day. Combined with whole genome sequencing analysis, it was revealed that its genome carries a more complete phenanthrene degradation pathway, including functional gene clusters related to the metabolism of PAHs, such as phd and nid. Meanwhile, intermediates such as phthalic acid were detected; it was determined that TJFP1 metabolizes phenanthrene via the phthalic acid pathway. Simulated contaminated soil experiments were also conducted, and the results showed that the removal rate of phenanthrene from the soil after 20 days of inoculation with the bacterial strain was about 3.7 times higher than that of the control group (natural remediation). At the same time from the soil physical and chemical properties and soil microbial community structure of two levels to explore the changes in different means of remediation, indicating that it can be successfully colonized in the soil, and as a dominant group of bacteria to play the function of remediation, verifying the environmental remediation function of the strains, for the actual inter-soil remediation to provide theoretical evidence. This study provides efficient strain resources for the bioremediation of PAH contamination.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114333/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Wastewater-Based Epidemiology for Pandemic Surveillance: Methodological Frameworks and Future Perspectives.","authors":"Weihe Zhu, Daxi Wang, Pengsong Li, Haohao Deng, Ziqing Deng","doi":"10.3390/microorganisms13051169","DOIUrl":"10.3390/microorganisms13051169","url":null,"abstract":"<p><p>Wastewater-based epidemiology (WBE) has emerged as a transformative approach for community-level health monitoring, particularly during the COVID-19 pandemic. This review critically examines the methodological framework of WBE systems through the following three core components: (1) sampling strategies that address spatial-temporal variability in wastewater systems, (2) comparative performance of different platforms in pathogen detection, and (3) predictive modeling integrating machine learning approaches. We systematically analyze how these components collectively overcome the limitations of conventional surveillance methods through early outbreak detection, asymptomatic case identification, and population-level trend monitoring. While highlighting technical breakthroughs in viral concentration methods and variant tracking through sequencing, the review also identifies persistent challenges, including data standardization, cost-effectiveness concerns in resource-limited settings, and ethical considerations in public health surveillance. Drawing insights from global implementation cases, we propose recommendations for optimizing each operational phase and discuss emerging applications beyond pandemic response. This review highlights WBE as an indispensable tool for modern public health, whose methodological refinements and cross-disciplinary integration are critical for transforming pandemic surveillance from reactive containment to proactive population health management.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12113820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a Sensitive and Visual Detection Platform for Viable <i>Salmonella</i> in Wastewater That Combines Propidium Monoazide with Recombinase Polymerase Amplification-CRISPR/Cas12a System.","authors":"Jiayin Liang, Xintian Sui, Yan Xu, Xiangqun Zheng, Lu Tan","doi":"10.3390/microorganisms13051166","DOIUrl":"10.3390/microorganisms13051166","url":null,"abstract":"<p><p>Urban sewage, aquaculture wastewater, and medical wastewater are significant reservoirs and transmission sources of <i>Salmonella</i>. Rapid detection of <i>Salmonella</i> is crucial for effectively reducing the risk of disease transmission and safeguarding public health. Differentiating viable <i>Salmonella</i> from inactivated cells presents significant challenges, affecting the accurate assessment of pathogen risks. Moreover, current detection methods face several limitations, including lengthy detection periods, high costs, and limited applicability, underscoring the need for rapid, sensitive, and visual detection diagnostic approaches. In this study, we combined propidium monoazide (PMA) with recombinase polymerase amplification (RPA) and clustered regularly spaced short palindromic repeats (CRISPR)/Cas12a systems to develop a rapid detection system for viable <i>Salmonella</i> targeting the <i>fim</i>Y gene. DNA of viable <i>Salmonella</i> was amplified and visually detected within 60 min and dead cells were effectively excluded. We assessed the specificity and sensitivity of the PMA-RPA-CRISPR/Cas12a assay. The results showed that the assay had a high level of specificity, with no reactions observed with other pathogens. The application of PMA has no effect on the sensitivity of RPA-CRISPR/Cas12a technology and the visibility of the fluorescence reporting system. We successfully detected viable <i>Salmonella</i> in wastewater with a minimum detection limit of 10¹ CFU/mL. In summary, the PMA-RPA-CRISPR/Cas12a system developed in this study allows for the rapid and visual detection of viable <i>Salmonella</i> in wastewater at concentrations as low as 10¹ CFU/mL. By integrating PMA with the RPA-CRISPR/Cas12a technology, this system offers valuable technical support for the efficient, sensitive, and clear detection of viable <i>Salmonella</i> in wastewater.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114456/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiological Profiles, Antibiotic Susceptibility Patterns and the Role of Multidrug-Resistant Organisms in Patients Diagnosed with Periprosthetic Joint Infection over 8 Years: Results from a Single-Center Observational Cohort Study from Romania.","authors":"Serban Dragosloveanu, Rares-Mircea Birlutiu, Bogdan Neamtu, Victoria Birlutiu","doi":"10.3390/microorganisms13051168","DOIUrl":"10.3390/microorganisms13051168","url":null,"abstract":"<p><p>This study examines temporal patterns in pathogens isolated from prosthetic joint infection (PJI) cases and antimicrobial resistance patterns at a Romanian orthopedic center. We have conducted a retrospective cohort study that included 674 patients undergoing hip or knee replacement revision surgery between January 2016 and December 2023. From these, 102 confirmed PJI cases requiring surgical intervention were selected for analysis. We isolated 27 microorganisms from acute PJI cultures and 82 from chronic PJIs. <i>Staphylococcus epidermidis</i> (33 cases, 30.3%; 95% CI 22.0-40.3) was the predominant pathogen, with coagulase-negative <i>Staphylococci</i> (22 cases, 20.18%; 95% CI 0.9-41.3) and Enterobacteriaceae (13 cases, 11.9%; 95% CI 6.4-18.3) also prevalent. Methicillin resistance was identified in 43.6% of coagulase-negative staphylococci and 45.5% of <i>Staphylococcus aureus</i> isolates. All Gram-positive isolates remained susceptible to vancomycin, linezolid, and tigecycline. Among Gram-negative bacilli, <i>Klebsiella oxytoca</i> and <i>Proteus mirabilis</i> showed resistance to third-generation cephalosporins, with phenotypic profiles suggestive of extended-spectrum β-lactamase (ESBL) production. All <i>Escherichia coli</i>, <i>Enterobacter</i> spp., and <i>Citrobacter freundii</i> strains were fully susceptible to tested agents, while <i>Pseudomonas aeruginosa</i> exhibited reduced susceptibility to ciprofloxacin, aztreonam, and imipenem. Among the isolated strains, 47 were multidrug-resistant (MDR), with <i>Staphylococcus aureus</i> accounting for the highest MDR count, including methicillin resistance. The distribution of microorganism types and MDR strains remained consistent throughout the study period, with no significant association between infection type and MDR strain presence or between infection site and microorganism presence except for a strong association between MDR strains and the type of microorganism (<i>p</i> < 0.05). The microbial profile and resistance patterns in PJIs have remained stable over eight years. Our observations do not suggest that MDR PJIs are more commonly acute cases, contrary to what has been highlighted in previous reports. The ongoing prevalence of MDR strains underscores the importance of targeted antimicrobial treatments based on local susceptibility profiles.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114344/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of PRV-1 in Atlantic Salmon and PRV-3 in Coho Salmon: Host-Specific Immune Responses and Apoptosis in Red Blood Cells.","authors":"Laura V Solarte-Murillo, Sebastián Salgado, Tomás Gatica, Juan Guillermo Cárcamo, Thomais Tsoulia, Maria K Dahle, Carlos Loncoman","doi":"10.3390/microorganisms13051167","DOIUrl":"10.3390/microorganisms13051167","url":null,"abstract":"<p><p>Fish red blood cells (RBCs) are nucleated, transcriptionally active, and key players in both gas transport and immune responses. They are the primary targets of <i>Orthoreovirus piscis</i> (PRV), the etiological agent of heart and skeletal muscle inflammation (HSMI), which includes three genotypes (PRV-1, PRV-2, and PRV-3), linked to circulatory disorders in farmed salmon. In Chile, PRV-3 affects the coho salmon (<i>Oncorhynchus kisutch</i>), but host-pathogen interactions remain poorly characterized. This study compared the interactions of PRV-3 in coho salmon and PRV-1 in Atlantic salmon (<i>Salmo salar</i>) using RBC infection models. RBCs were isolated from healthy juvenile salmon (n = 3) inoculated with either PRV-1 (Ct = 18.87) or PRV-3 (Ct = 21.86). Poly I:C (50 µg/mL) was used as a positive control for the antiviral response. Cells were monitored for up to 14 days post-infection (dpi). PRV-3 infection in coho salmon RBCs caused significant metabolic disruption, apoptosis from 7 dpi, and correlated with increasing viral loads. In contrast, PRV-1 infection in Atlantic salmon RBCs showed limited apoptosis and maintained cell viability. Coho salmon RBCs upregulated <i>rig-i</i>, <i>mx</i>, and <i>pkr</i> transcripts, indicating activation of the type I interferon pathway, whereas Atlantic salmon RBCs exhibited a more attenuated response. PRV-3 induced notable morphological changes in coho salmon RBCs, although neither PRV-3 nor PRV-1 caused hemolysis. These findings highlight species-specific differences in RBC responses to PRV infection and provide new insights into the pathogenesis of PRV-3 and PRV-1.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12113769/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity of Antifungal Properties in Bacterial Isolates from Different Plant Species Growing Across Uzbekistan.","authors":"Mukhlisa K Shodmonova, Dono A Muhammadova, Ilkham S Aytenov, Marufbek Z Isokulov, Tohir A Bozorov, Daoyuan Zhang, Ozodbek S Abduraimov, Sojida M Murodova, Fazliddin A Melikuziev, Bekhruz O Ochilov, Sodir K Meliev","doi":"10.3390/microorganisms13051161","DOIUrl":"10.3390/microorganisms13051161","url":null,"abstract":"<p><p>Plant-associated bacteria play a crucial role in protecting plants from pathogens, yet the diversity and antagonistic potential of these bacteria across different plant species remain underexplored, especially in central Asia. To investigate the competitive dynamics between phytopathogenic fungi and plant-associated bacteria, we collected stem and root samples from 50 plant species across nine regions of Uzbekistan. A total of 3355 bacterial isolates were obtained (1896 from roots and 1459 from shoots) and screened for antifungal activity against six fungal pathogens, resulting in 432 antagonistic isolates. These were identified through 16S rDNA sequencing, revealing 65 bacterial species across three phyla: Firmicutes, Proteobacteria, and Actinobacteria, predominantly in the respective families <i>Bacillaceae</i>, <i>Pseudomonadaceae</i>, and <i>Caryophanaceae.</i> The plant <i>Salsola vvedenskii</i> hosted the highest diversity of antagonists (26 species), while other species harbored fewer. Plant species showed strong associations with specific bacterial communities, with 14 plant species each hosting unique antagonists. Enzymatic profiling revealed functional diversity, with <i>Bacillus</i> species producing protease, cellulase, and lipase activities, while <i>Pseudomonas</i> species excelled in xylanase, glucanase, and cellobiase production. <i>B. mojavensis</i> 9r-29 stood out by producing all six enzymes. These findings underscore the ecological diversity and biocontrol potential of plant-associated bacteria in natural ecosystems, offering promising candidates for sustainable plant protection strategies.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114544/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprotection with <i>Saccharomyces cerevisiae</i>: A Promising Strategy.","authors":"Fatima El Dana, Vanessa David, Raphaëlle Tourdot-Maréchal, Salem Hayar, Marie-Charlotte Colosio, Hervé Alexandre","doi":"10.3390/microorganisms13051163","DOIUrl":"10.3390/microorganisms13051163","url":null,"abstract":"<p><p>Bioprotection in winemaking refers to the use of naturally occurring microorganisms-mainly non-<i>Saccharomyces</i> yeasts-to inhibit the growth of spoilage microbes and reduce the need for chemical preservatives like sulfur dioxide (SO₂). Numerous studies have demonstrated the benefits of non-<i>Saccharomyces</i> as bioprotectants. However, the use of <i>Saccharomyces cerevisiae</i> as a bioprotectant has been studied very little. Furthermore, it can offer many advantages for the production of sulfite-free wines. To test if <i>S. cerevisiae</i> could be used in bioprotection, we compared the ability of different strains to inhibit the growth of <i>Brettanomyces bruxellensis</i> and <i>Hanseniaspora uvarum</i>. Among the strains tested, the <i>S. cerevisiae</i> Sc54 strain isolated from the vineyard of the Bekaa plain was selected. To investigate its mechanisms of action, we analyzed its metabolite production, including acetic acid and ethanol. Taking into account the low levels of these metabolites and the lack of similar inhibition patterns in media supplemented with acetic acid and ethanol, it appears that other factors contribute to its antagonistic properties. Nutrient competition was ruled out as a factor, as the growth inhibition of <i>B. bruxellensis</i> and <i>H. uvarum</i> occurred rapidly within the first 24 h of co-culture. In this study, we explored the role of the <i>S. cerevisiae</i> killer toxin (Sc54Kt) as a bioprotective agent against <i>H. uvarum</i> and <i>B. bruxellensis</i> spoilage yeasts. Purification procedures with ethanol allowed the extraction of Sc54Kt, yielding two concentrations (0.185 and 0.5 mg/mL). Remarkably, semi-purified Sc54Kt exhibited inhibitory effects at both concentrations under winemaking conditions, effectively controlling the growth and metabolic activity of the target spoilage yeasts. Overall, these findings demonstrate that <i>S. cerevisiae</i> Sc54 not only exerts a strong bioprotective effect but also contributes to improving the quality of wine. The results suggest that <i>S. cerevisiae</i> Sc54 is a promising bioprotective agent for mitigating spoilage yeasts in winemaking, offering a natural and effective alternative to conventional antimicrobial strategies.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114017/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial Heterogeneity in Soil Microbial Communities Impacts Their Suitability as Bioindicators for Evaluating Productivity in Agricultural Practices.","authors":"Guoqiang Li, Xuanjing Li, Ting Jin, Muyilan Jiang, Peng Shi, Gehong Wei","doi":"10.3390/microorganisms13051160","DOIUrl":"10.3390/microorganisms13051160","url":null,"abstract":"<p><p>Soil microorganisms are increasingly recognized as critical regulators of farmland soil fertility and crop productivity. However, the impacts of spatial heterogeneity in soil microbial communities on bioindicators for evaluating agricultural practices remain poorly understood and warrant further validation. Through field experiments, this study investigated the differential effects of agricultural practice treatments on soil properties and bacterial communities between two main farmland soil compartments: intra-row and inter-row. Additionally, we explored the potential correlations between key taxa and soil properties, as well as maize biomass. Results revealed marked disparities in soil properties, bacterial community compositions, and co-occurrence network patterns between intra-row and inter-row soils. Agricultural practice treatments exerted significant impacts on bacterial community structures and network topological features in both intra-row and inter-row soils. Subsequent correlation analysis demonstrated strong relationships between soil properties and most keystone species. In addition, 42 and 41 indicator species were identified in intra-row and inter-row soils, respectively, including shared genera such as <i>Solirubrobacter</i>, <i>Blastococcus</i>, <i>Iamia</i>, <i>Conexibacter</i>, and <i>Lysobacter</i>. Notably, 22 key indicator species in intra-row soils displayed significant positive/negative correlations with maize biomass, whereas only 4 key indicator species showed negative correlations in inter-row soils. These findings highlight differential responses of bacterial communities to agricultural practices in distinct soil compartments. The intra-row soils harbored more bacterial taxa significantly associated with maize biomass, while the inter-row soils better reflected the effects of agricultural interventions. This study confirms the spatial variability of microbial communities as effective bioindicators for evaluating agricultural practice strategies. Identification of compartment-specific indicators provides novel microbiological insights into supporting precision agriculture practices.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12113728/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}