Microorganisms最新文献

{"title":"Reporting of Salmonellosis Markedly Declined in Finland During 28 Years of Surveillance, 1995-2022.","authors":"Kristiina Suominen, Jukka Ollgren, Elina Leinonen, Ruska Rimhanen-Finne","doi":"10.3390/microorganisms13030693","DOIUrl":"10.3390/microorganisms13030693","url":null,"abstract":"<p><p>Salmonellosis is the second most common foodborne bacterial gastroenteritis in humans in Finland and worldwide. In Finland, a national salmonella control program covering cattle, pigs, and poultry, as well as the meat and eggs obtained from them, has been in place since the country joined the EU in 1995. To identify trends in the incidence of salmonellosis and to reflect on their causes, we analyzed <i>Salmonella</i> case data from the Finnish Infectious Disease Register (FIDR) from 1995 to 2022 and outbreak data from the national food and waterborne outbreak register (the FWO register) in the period 2010-2022. From 1995-1999 to 2015-2019, the incidence of salmonellosis decreased by 66% and 63% for domestic and travel-related cases, respectively. Most salmonellosis cases (72%) were travel-related, and, of them, 27% were infected in Southeast Asia. The most common serovars were <i>S</i>. Typhimurium and <i>S</i>. Enteritidis (38% and 19% of domestic cases, and 7% and 39% of travel-related cases). During 2010-2022, <i>Salmonella</i> sp. was reported as a cause in 31 foodborne outbreaks. In 14 of them, the source was identified at foodstuff level, and 12 sources were of foreign origin. The results of this study indicate that the national salmonella control program may have prevented domestic human infections in Finland.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945640/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Genomic Characteristics of Potential Probiotics: Two <i>Streptococcus salivarius</i> Isolates from a Healthy Individual in China.","authors":"Mingyue Sun, Qian Li, Feiran Zhang, Ding Yao, Wenhua Huang, Qingyu Lv, Hua Jiang, Decong Kong, Yuhao Ren, Shaolong Chen, Yongqiang Jiang, Peng Liu","doi":"10.3390/microorganisms13030694","DOIUrl":"10.3390/microorganisms13030694","url":null,"abstract":"<p><p>The isolation and characterization of novel probiotics from dairy products, fermented foods, and the gut have gained significant attention. In particular, <i>Streptococcus salivarius</i> shows promise for use in oral probiotic preparations. In this study, we isolated two strains of <i>S. salivarius</i>-S.82.15 and S.82.20-from the oral cavity of a healthy individual. These strains exhibited distinct antimicrobial profiles. We thoroughly assessed the morphology and growth patterns of both strains and confirmed auto-aggregation and hemolytic activity. Through comprehensive genomic analysis, we found notable strain differences within the same bacterial species isolated from the same individual. Notably, the presence or absence of plasmids varied between the two strains. The genome of S.82.15 spans 2,175,688 bps and contains 1994 coding DNA sequences (CDSs), while S.82.20 has a genome size of 2,414,610 bps, a GC content of 40.62%, and 2276 annotated CDSs. Both strains demonstrated antibacterial activity against Group A <i>Streptococcus</i> (GAS), <i>Micrococcus. luteus</i>, and <i>Porphyromonas gingivalis</i>. To investigate the antibacterial properties further, we identified a gene cluster of salivaricin 9 on the plasmid of S.82.20 and a <i>blp</i> gene family on the chromosomes of both S.82.15 and S.82.20. Moreover, the gene expression of the <i>blp</i> family was upregulated when the isolated strains were co-cultured with GAS.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial Contamination on High-Touch Surfaces in Outpatient Clinics: Identification of Bacterial Strains from Areas of Patient and Medical Staff Occupancy.","authors":"Karolina Prasek, Iwona Kiersnowska, Jadwiga Wójkowska-Mach, Anna Różańska, Dorota Romaniszyn, Ewelina Foryciarz, Lucyna Barbara Kwiećkowska, Edyta Krzych-Fałta","doi":"10.3390/microorganisms13030698","DOIUrl":"10.3390/microorganisms13030698","url":null,"abstract":"<p><p>Clinics and hospitals inherently increase the risk of adverse events, including hospital-acquired infections (HAIs) transmitted between healthcare personnel and patients. This study aimed to identify bacterial strains present on frequently touched surfaces in outpatient clinics used by patients as well as medical and non-medical personnel. This study was conducted in four outpatient care centers located in two major cities in Poland. A total of 85 samples were collected from frequently touched surfaces, including 53 samples from areas accessed by patients and 32 samples from surfaces used by medical staff. A statistically significant increase in moderate-to-heavy growth was observed in samples containing microbiota compared to those containing other microorganisms (<i>p</i> = 0.003). Similarly, a higher prevalence of spore-forming bacteria was noted compared to non-spore-forming bacteria (<i>p</i> = 0.001). A significant difference was also observed between samples with no or scant growth versus those with moderate-to-heavy growth in both the microbiota and other microorganism groups (<i>p</i> = 0.003), as well as between the spore-forming and non-spore-forming groups (<i>p</i> = 0.001). The findings of this study prompted revisions in cleaning procedures. The frequency of training for medical staff was increased, and systematic quality control of the cleaning company's performance was implemented.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating Cover Crops and Manure to Boost Goji Berry Yield: Responses of Soil Physicochemical Properties and Microbial Communities.","authors":"Haonan Chen, Fang Wang, Yamiao Gao, Yaran Ma, Lizhen Zhu, Xiongxiong Nan","doi":"10.3390/microorganisms13030696","DOIUrl":"10.3390/microorganisms13030696","url":null,"abstract":"<p><p>A sustainable Goji berry (<i>Lycium barbarum</i> L.) planting system that integrates forage radish cover crops (<i>Raphanus sativus</i> L.) and animal manure has been established in northwestern China. This study investigated the effects of different cropping systems and manure application levels on soil physicochemical properties, microbial community structure, and <i>L. barbarum</i> yield under field conditions. A split-plot design was used, with the main-plot treatments consisting of two cropping systems and the sub-plot treatments involving three manure application levels. The results showed that compared to <i>L. barbarum</i> monocropping, cover cropping with <i>R. sativus</i> led to a decrease in soil bulk density (1.90%) and increase in soil electrical conductivity (11.5%), nutrient contents (total N and available N, P, and K: 30.3-138%), and microbial biomass (C: 79.0%; N: 184%). Cover cropping additionally enhanced the community diversity and richness of soil bacteria. Beta-diversity analysis revealed significant differences in bacterial rather than fungal community composition among various treatments. The bacterial network showed a lower ratio of positive to negative correlations and reduced complexity in response to cover cropping, which contrasted with fungal network patterns. Integration of cover cropping and medium manure application increased fruit yield by 8.71%. Cover crops and manure influenced soil microbial diversity mainly through their positive effects on soil total and available N contents.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944604/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Composition, Distribution and Mobility Potential of the Antibiotic Resistome in Sediments from the East China Sea Revealed by Metagenomic Analysis.","authors":"Xiaozhong Chen, Long Gao, Yanxue Kou, Xiaoxuan Wang, Xintong Li, Hui He, Min Wang","doi":"10.3390/microorganisms13030697","DOIUrl":"10.3390/microorganisms13030697","url":null,"abstract":"<p><p>Marine sediments are recognized as crucial reservoirs of antibiotic resistance genes (ARGs). However, the antibiotic resistome in sediments of the East China Sea, an area heavily impacted by human activities, has not been thoroughly studied. Here, we conducted a systematic investigation into the antibiotic resistome in these sediments using metagenomic analysis. Overall, we detected eighty ARG subtypes and nineteen ARG types. Beta-lactams were the dominant ARG type, and Gammaproteobacteria was the main ARG host in this study. Mobile genetic elements (MGEs) were not major drivers of ARG profiles. Although the ARG host communities significantly differed between the spring and autumn (<i>p</i> < 0.05), the antibiotic resistome remained stable across the two seasons. The assembly of ARGs and their hosts was governed by stochastic processes, and a high ratio of stochastic processes implied its crucial role in the assembly and stabilization of the antibiotic resistome. Co-occurrence network analysis revealed an important role of Deltaproteobacteria in the stabilization of ARG profiles across seasons. Environmental parameters (e.g., temperature and density) played certain roles in the stabilization of the antibiotic resistome between spring and autumn. Moreover, nine human pathogen bacteria (HPB) were detected in this study. We also found that the health risks caused by ARGs were relatively higher in the spring. Our results will provide a strong foundation for the development of targeted management strategies to mitigate the further dissemination and spread of ARGs in marine sediments.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Minute Virus of Canines (MVC) Activates the RhoA/ROCK1/MLC2 Signal Transduction Pathway Resulting in the Dissociation of Tight Junctions and Facilitating Occludin-Mediated Viral Infection.","authors":"Xiang Ren, Zhiping Hei, Kai Ji, Yan Yan, Chuchu Tian, Yin Wei, Yuning Sun","doi":"10.3390/microorganisms13030695","DOIUrl":"10.3390/microorganisms13030695","url":null,"abstract":"<p><p>The Minute Virus of Canines (MVC), belonging to the genus <i>Bocaparvovirus</i> within the family <i>Parvoviridae</i>, is associated with enteritis and embryonic infection in neonatal canines. Viral attachment to host cells is a critical step in infection, and viral protein 2 (VP2) as an important structural protein of MVC influences host selection and infection severity. Nevertheless, little is known about the interaction between VP2 protein and host cells. In this study, we identified that VP2 directly interacts with the kinase domain of RhoA-associated protein kinase 1 (ROCK1) by using mass spectrometry and immunoprecipitation approach and demonstrated that the RhoA/ROCK1/myosin light chain 2 (MLC2) signaling pathway was activated during the early stage of MVC infection in Walter Reed canine cell/3873D (WRD) cells. Further studies indicated that RhoA/ROCK1-mediated phosphorylation of MLC2 triggers the contraction of the actomyosin ring, disrupts tight junctions, and exposes the tight junction protein Occludin, which facilitates the interaction between VP2 and Occludin. Specific inhibitors of RhoA and ROCK1 restored the MVC-induced intracellular translocation of Occludin and the increase in cell membrane permeability. Moreover, the two inhibitors significantly reduced viral protein expression and genomic copy number. Collectively, our study provides the first evidence that there is a direct interaction between the structural protein VP2 of MVC and ROCK1, and that the tight junction protein Occludin can serve as a potential co-receptor for MVC infection, which may offer new targets for anti-MVC strategies.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944487/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case Report: Long-Term Follow-Up of Visceral Leishmaniasis and HIV Coinfected Patients Without Relapse: Lymphoproliferative Response After Stimulation with Soluble <i>Leishmania</i> Antigen.","authors":"Begoña Monge-Maillo, Daniel Roger-Zapata, Fernando Dronda, Eugenia Carrillo, Javier Moreno, María Dolores Corbacho-Loarte, Diego Gayoso Cantero, Oihane Martín, Sandra Chamorro-Tojeiro, Jose A Perez-Molina, Francesca Norman, Marta González-Sanz, Rogelio López-Vélez","doi":"10.3390/microorganisms13030686","DOIUrl":"10.3390/microorganisms13030686","url":null,"abstract":"<p><p>Highly active antiretroviral therapy (HAART) has reduced the incidence of VL/HIV dramatically. However, HAART only partially prevents relapses, with one-year relapse rates ranging from 30 to 60%. Consequently, secondary prophylaxis is recommended for patients with <200 CD4+ cells/μL. In clinical practice, characterizing cellular immune response could help estimate the risk of relapse in VL/HIV coinfected patients. In this study, the lymphoproliferative response after stimulation with soluble <i>Leishmania</i> antigen was assessed in 2022 and 2023 in three cases of VL/HIV coinfection with long-term follow-up (17, 8 and 19 years). PCR and rK-39 results for <i>Leishmania</i>, HIV viral load, CD4 cell count, proliferation index, IFN-γ, IL-2, IP-10, IL-10 and TNF-α were determined. Heterogeneous results were obtained, with only one patient having developed specific cellular immunity against <i>Leishmania</i>. No cases of relapse were observed. The heterogeneity of lymphoproliferative test results in the three cases described highlights the need to identify surrogate markers of cure to guide maintenance or withdrawal of prophylaxis.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944632/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Antimicrobial, Antioxidant and Extracellular Enzymatic Activities of Culturable Endophytic Fungi Isolated from the Leaves of <i>Kirkia acuminata</i> Oliv.","authors":"Sagwadi Kubayi, Raymond Tshepiso Makola, Khumiso Dithebe","doi":"10.3390/microorganisms13030692","DOIUrl":"10.3390/microorganisms13030692","url":null,"abstract":"<p><p>Fungal endophytes of medicinal plants produce diverse secondary metabolites and extracellular enzymes with therapeutic and biotechnological potential. However, the biological and biotechnological potential of fungal endophytes from South African medicinal plants remain relatively underexplored. In this study, the antimicrobial, antioxidant, anti-inflammatory and extracellular enzymatic capabilities of five fungal endophytes previously isolated from the leaves of <i>Kirkia acuminata</i> Oliv. were investigated. Sequencing of the internal transcribed spacer (ITS) regions revealed that the isolates belonged to the genera <i>Setosphaeria</i>, <i>Diaporthe</i> and <i>Corynespora</i>. The broth micro-dilution assay and the Folin-Ciocalteau reagent method were used to assess the antibacterial activity and the total phenolic content (TPC) of the fungal endophytes' ethyl acetate crude extracts (CEs), respectively. The antioxidant activity was assessed using the ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assays. The influence of the CE of the <i>Setosphaeria rostrata</i> KaL-4 on the viability and LPS-induced interleukin-6 (IL-6) production in Raw 264.7 macrophages was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and an ELISA, respectively. The ability of the isolates to produce extracellular proteases, laccases and peroxidases was also determined. The CEs displayed antimicrobial activity with MICs ranging from 0.63 to 1.25 mg/mL and reducing power and scavenging activity ranging from 40% to 18% and from 60% to 48%, respectively. The <i>S. rostrata</i> KaL-4 CE possessed the highest TPC and demonstrated dose-dependent cytotoxicity. The CE further demonstrated a significant reduction in IL-6 production at a concentration of 0.75 µg/mL. Only one isolate demonstrated the ability to produce proteases with an enzymatic index (EI) of 0.66, while laccases (EI range of 0.14 to 1.15) and peroxidases were produced by all of the isolates. These findings suggest that fungal endophytes from South African medicinal plants are promising sources of bioactive compounds and industry-significant extracellular enzymes.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Butyric Acid Supplementation on the Gut Microbiome and Growth Performance of Weanling Pigs Fed a Low-Crude Protein, Propionic Acid-Preserved Grain Diet.","authors":"Kathryn Ruth Connolly, Torres Sweeney, Marion T Ryan, Stafford Vigors, John V O'Doherty","doi":"10.3390/microorganisms13030689","DOIUrl":"10.3390/microorganisms13030689","url":null,"abstract":"<p><p>Reducing crude protein (CP) in weaner pig diets lowers post-weaning diarrhoea risk but may impair growth performance. This study aimed to identify the beneficial effects of organic acid (OA)-preserved grain and butyric acid supplementation on gut health and growth in low-CP diets. At harvest, grain was divided into two batches: one dried at 65 °C, the other treated with a propionic acid. Ninety-six piglets (28 days old) were assigned to four treatments: (1) dried grain, (2) OA-preserved grain, (3) dried grain + 3% butyric acid, and (4) OA-preserved grain + 3% butyric acid. On day 8, microbial composition, inflammatory markers, volatile fatty acids, and intestinal morphology were assessed. The OA-preserved grain improved feed conversion ratio (<i>p</i> < 0.05) increased beneficial gut bacteria (<i>p</i> < 0.01), elevated caecal butyrate (<i>p</i> < 0.05), reduced jejunal <i>CXCL8</i> expression (<i>p</i> < 0.05), and enhanced nutrient digestibility (<i>p</i> < 0.01). Butyric acid reduced feed intake (<i>p</i> < 0.05), improved nutrient digestibility (<i>p</i> < 0.01), decreased colonic <i>Proteobacteria</i> (<i>p</i> < 0.05), and increased colonic propionate and butyrate (<i>p</i> < 0.01). Combining OA-preserved grain with butyric acid elevated ileal <i>Proteobacteria</i> and <i>Pasteurellaceae</i> (<i>p</i> < 0.05). In conclusion, while OA-preserved grain improves feed efficiency, nutrient digestibility, and gut microbiota, supplementing butyric acid enhances nutrient digestibility but reduces feed intake, and their combination may disrupt the microbial balance.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11946337/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic Resistance and Virulence Determinants of <i>Pseudomonas aeruginosa</i> Isolates Cultured from Hydrocarbon-Contaminated Environmental Samples.","authors":"Chioma Lilian Ozoaduche, Balázs Libisch, Daniel Itoro, Iyore Blessing Idemudia, Katalin Posta, Ferenc Olasz","doi":"10.3390/microorganisms13030688","DOIUrl":"10.3390/microorganisms13030688","url":null,"abstract":"<p><p>Crude oil and its derivates are among the most important environmental pollutants, where <i>P. aeruginosa</i> strains producing AlkB1 and AlkB2 alkane hydroxylases are often involved in their biodegradation. The aim of this study was to analyze antibiotic resistance and virulence determinants of a <i>P. aeruginosa</i> isolate cultured from a hydrocarbon-contaminated soil sample from Ogoniland, Nigeria, and to compare its characteristics with <i>P. aeruginosa</i> isolates cultured worldwide from hydrocarbon-contaminated environments or from clinical samples. Using the ResFinder reference database, a <i>catB7</i> chloramphenicol acetyltransferase gene, an <i>ampC</i>-type PDC β-lactamase gene, and an OXA-50 type β-lactamase gene were identified in all <i>P. aeruginosa</i> strains analyzed in this study. In some of these <i>P. aeruginosa</i> strains, loss-of-function mutations were detected in the regulatory genes <i>mexR</i>, <i>nalC</i>, or <i>nalD</i>, predicting an efflux-mediated acquired antibiotic-resistance mechanism. Several <i>P. aeruginosa</i> sequence types that were associated with oil-contaminated environments have also been cultured from human clinical samples worldwide, including sequence types ST532, ST267, ST244, and ST1503. Our findings also indicate that environmental <i>P. aeruginosa</i> may serve as the source of human infections, warranting further studies from a One Health perspective about the application of <i>P. aeruginosa</i> for the in situ bioremediation of hydrocarbon-contaminated sites.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}